MD09G1134100.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000577
Physical Location & Seq
Forward (+)
10317747 .. 10318256
510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1134100.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGTAGTGGTACCTCTAGCTAGCAACTATAAAAGCATAGTTCTTCTGGTGTTTTTTTTTCACAGAGAATTAAAATTTAATTTTCTTCAAATTAACGAGCAATATAGCATGGCTTGGTTCAATGGAAGTTCAGCACTGCTGCTAGTCACGCTGGTTTTGTTCACAGTCACCATTTGTGATGCATTCAAGACAAGATATGTTAGAGTCACAAATAATTTGGAGGGGGGCCAGACCCTCAGCCTTCACTGTAAATCCGAGGACGATGATCTTGGTCTCCAAACGCTCGCGACGAACGCCTCCTTTGAATTCCACTTCAAACCTAGCTTTATCACCATAACGAAGTTTGATTGCAGTTTTGAGTGGCCAGGTGCATCGCACTGGTTCGAAATATATAATCATGAGAGAGAGAAAGTATATTGTAGCAAGTGTTACTGGAGTGTTACCACAGCTGCTCCGTGCAGGTACGACTGGGACGAGCAAAAGTACAGCAAATGCTTTATGTGGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

170

Amino Acids

19.82

Weight (kDa)

6.19

Isoelectric Point (pI)

14.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 67 - 168 1.1e-25 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16960 AT3G16970 AT3G17080 AT4G16195 AT5G12060 AT5G12070
fragaria_vesca FvH4_2g16811 FvH4_4g02030 FvH4_4g02050 FvH4_4g02160 FvH4_4g05620
malus_domestica MD04G1130600.v1.1 MD07G1006200.v1.1 MD07G1270000.v1.1 MD09G1134100.v1.1 MD09G1134200.v1.1 MD10G1085700.v1.1 MD17G1052500.v1.1 MD17G1052600.v1.1 MD17G1052700.v1.1 MD17G1123000.v1.1
prunus_persica Prupe.1G026600_v2.0.a1 Prupe.1G049500_v2.0.a1 Prupe.1G055500_v2.0.a1 Prupe.1G055600_v2.0.a1 Prupe.1G057000_v2.0.a1 Prupe.1G057100_v2.0.a1 Prupe.1G057200_v2.0.a1 Prupe.1G057300_v2.0.a1 Prupe.1G058100_v2.0.a1 Prupe.8G012700_v2.0.a1
pyrus_communis pycom17g05060 pycom17g11360
rosa_chinensis RchiOBHm_Chr3g0496401 RchiOBHm_Chr4g0389011 RchiOBHm_Chr4g0389231 RchiOBHm_Chr4g0389421 RchiOBHm_Chr4g0396771 RchiOBHm_Chr4g0396781 RchiOBHm_Chr4g0396791 RchiOBHm_Chr4g0399631 RchiOBHm_Chr4g0399641 RchiOBHm_Chr5g0071851 RchiOBHm_Chr6g0268511
rosa_laevigata RLG00000009249 RLG00000009256 RLG00000009505 RLG00000009983 RLG00000009984 RLG00000013253
rosa_multiflora Rmu_sc0000487.1_g000011 Rmu_sc0002404.1_g000026
rosa_roxburghii Rroxscaffold_5G00335430 Rroxscaffold_5G00341610
rosa_rugosa Rorug03G0282200 Rorug03G0347600 Rorug04G0017800 Rorug04G0017800 Rorug05G0227600 Rorug06G0042400 Rorug06G0042500
rosa_samantha Rh3AG328900 Rh4AG027600 Rh4BG093100 Rh4CG029700 Rh4CG077600 Rh4CG077800 Rh4CG104100 Rh4CG104200 Rh4DG020800 Rh4DG021100 Rh4DG021200 Rh4DG067000 Rh4DG067100 Rh6AG162000 Rh6BG166300
rosa_wichuraiana Rw4G002000 Rw4G005780 Rw4G005790 Rw4G007880 Rw6G013990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 450
Acc65I GGTACC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 10
AccII CGCG 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 362
AcsI RAATTY 2 cut(s) 74, 305
AfaI GTAC 3 cut(s) 12, 464, 485
AgsI TTSAA 5 cut(s) 89, 121, 187, 305, 316
AjnI CCWGG 1 cut(s) 364
AluBI AGCT 3 cut(s) 20, 324, 449
AluI AGCT 3 cut(s) 20, 324, 449
Alw26I GTCTC 1 cut(s) 278
AoxI GGCC 2 cut(s) 226, 362
ApeKI GCWGC 2 cut(s) 139, 449
ApoI RAATTY 2 cut(s) 74, 305
Asp718I GGTACC 1 cut(s) 10
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 2 cut(s) 160, 322
AsuII TTCGAA 1 cut(s) 384
AsuNHI GCTAGC 1 cut(s) 20
BalI TGGCCA 1 cut(s) 364
BanI GGYRCC 1 cut(s) 10
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 2 cut(s) 126, 436
BciT130I CCWGG 1 cut(s) 366
BcoDI GTCTC 1 cut(s) 278
BfaI CTAG 4 cut(s) 17, 21, 143, 321
BfuAI ACCTGC 1 cut(s) 450
BisI GCNGC 2 cut(s) 140, 450
BlsI GCNGC 2 cut(s) 141, 451
Bme1390I CCNGG 1 cut(s) 366
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 2 cut(s) 12, 227
BmrFI CCNGG 1 cut(s) 366
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 2 cut(s) 169, 380
BmtI GCTAGC 1 cut(s) 24
BmuI ACTGGG 1 cut(s) 478
BpmI CTGGAG 1 cut(s) 454
Bpu10I CCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 255
BsaXI ACNNNNNCTCC 2 cut(s) 436, 466
Bse1I ACTGG 3 cut(s) 383, 437, 473
BseBI CCWGG 1 cut(s) 366
BseDI CCNNGG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 250
BseNI ACTGG 3 cut(s) 383, 437, 473
BseXI GCAGC 2 cut(s) 126, 436
BsgI GTGCAG 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 287
BshFI GGCC 2 cut(s) 228, 364
BshNI GGYRCC 1 cut(s) 10
BslFI GGGAC 1 cut(s) 485
BsmAI GTCTC 1 cut(s) 278
BsmFI GGGAC 1 cut(s) 485
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 2 cut(s) 228, 364
Bso31I GGTCTC 1 cut(s) 278
Bsp119I TTCGAA 1 cut(s) 384
Bsp143I GATC 1 cut(s) 265
Bsp68I TCGCGA 1 cut(s) 287
BspANI GGCC 2 cut(s) 228, 364
BspCNI CTCAG 1 cut(s) 249
BspFNI CGCG 1 cut(s) 287
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 2 cut(s) 12, 227
BspMI ACCTGC 1 cut(s) 450
BspOI GCTAGC 1 cut(s) 24
BspT104I TTCGAA 1 cut(s) 384
BspT107I GGYRCC 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 278
BsrI ACTGG 3 cut(s) 383, 437, 473
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 2 cut(s) 166, 248
BstBI TTCGAA 1 cut(s) 384
BstC8I GCNNGC 2 cut(s) 22, 285
BstDEI CTNAG 1 cut(s) 236
BstFNI CGCG 1 cut(s) 287
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 1 cut(s) 265
BstMWI GCNNNNNNNGC 2 cut(s) 148, 293
BstNI CCWGG 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 364
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 2 cut(s) 126, 436
BsuRI GGCC 2 cut(s) 228, 364
BtgZI GCGATG 1 cut(s) 357
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 3 cut(s) 134, 244, 376
BtuMI TCGCGA 1 cut(s) 287
BveI ACCTGC 1 cut(s) 450
Cac8I GCNNGC 2 cut(s) 22, 285
CciI TCATGA 1 cut(s) 397
Cfr13I GGNCC 1 cut(s) 226
Csp6I GTAC 3 cut(s) 11, 463, 484
CviAII CATG 2 cut(s) 109, 398
CviJI RGCY 7 cut(s) 20, 113, 228, 240, 324, 364, 449
CviKI_1 RGCY 7 cut(s) 20, 113, 228, 240, 324, 364, 449
CviQI GTAC 3 cut(s) 11, 463, 484
DdeI CTNAG 1 cut(s) 236
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 278
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 364
EcoT22I ATGCAT 1 cut(s) 184
FaeI CATG 2 cut(s) 112, 401
FaqI GGGAC 1 cut(s) 485
FatI CATG 2 cut(s) 108, 397
Fnu4HI GCNGC 2 cut(s) 140, 450
Fsp4HI GCNGC 2 cut(s) 140, 450
FspBI CTAG 4 cut(s) 17, 21, 143, 321
GluI GCNGC 2 cut(s) 140, 450
GsuI CTGGAG 1 cut(s) 454
HaeIII GGCC 2 cut(s) 228, 364
Hin1II CATG 2 cut(s) 112, 401
HinfI GANTC 1 cut(s) 204
HphI GGTGA 2 cut(s) 160, 322
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 1 cut(s) 256
Hpy188III TCNNGA 3 cut(s) 187, 286, 398
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 2 cut(s) 166, 248
HpyCH4V TGCA 4 cut(s) 182, 351, 371, 459
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 293
HpyF3I CTNAG 1 cut(s) 236
Hsp92II CATG 2 cut(s) 112, 401
KpnI GGTACC 1 cut(s) 14
Kzo9I GATC 1 cut(s) 265
LmnI GCTCC 1 cut(s) 457
LpnPI CCDG 9 cut(s) 32, 137, 242, 351, 364, 378, 418, 445, 454
Lsp1109I GCAGC 2 cut(s) 126, 436
LweI GCATC 2 cut(s) 169, 380
MaeI CTAG 4 cut(s) 17, 21, 143, 321
MaeIII GTNAC 5 cut(s) 145, 166, 205, 428, 439
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 2 cut(s) 35, 77
MlsI TGGCCA 1 cut(s) 364
MluCI AATT 6 cut(s) 68, 74, 79, 90, 214, 305
MluNI TGGCCA 1 cut(s) 364
MlyI GAGTC 1 cut(s) 213
MnlI CCTC 5 cut(s) 24, 214, 245, 250, 307
Mox20I TGGCCA 1 cut(s) 364
Mph1103I ATGCAT 1 cut(s) 184
MscI TGGCCA 1 cut(s) 364
MseI TTAA 4 cut(s) 71, 78, 93, 508
Msp20I TGGCCA 1 cut(s) 364
MspA1I CMGCKG 1 cut(s) 449
MspR9I CCNGG 1 cut(s) 366
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 1 cut(s) 366
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 2 cut(s) 148, 293
NdeII GATC 1 cut(s) 265
NheI GCTAGC 1 cut(s) 20
NlaIII CATG 2 cut(s) 112, 401
NlaIV GGNNCC 2 cut(s) 12, 227
NmuCI GTSAC 3 cut(s) 145, 166, 205
NruI TCGCGA 1 cut(s) 287
NsiI ATGCAT 1 cut(s) 184
NspV TTCGAA 1 cut(s) 384
PagI TCATGA 1 cut(s) 397
PcsI WCGNNNNNNNCGW 2 cut(s) 287, 471
PctI GAATGC 1 cut(s) 182
PkrI GCNGC 2 cut(s) 141, 451
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 364
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 2 cut(s) 12, 227
PspPI GGNCC 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 449
RruI TCGCGA 1 cut(s) 287
RsaI GTAC 3 cut(s) 12, 464, 485
RsaNI GTAC 3 cut(s) 11, 463, 484
SaqAI TTAA 4 cut(s) 71, 78, 93, 508
SatI GCNGC 2 cut(s) 140, 450
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 1 cut(s) 366
SetI ASST 7 cut(s) 16, 22, 322, 326, 370, 451, 464
SfaNI GCATC 2 cut(s) 169, 380
SfuI TTCGAA 1 cut(s) 384
Sse9I AATT 6 cut(s) 68, 74, 79, 90, 214, 305
SspMI CTAG 4 cut(s) 17, 21, 143, 321
StyD4I CCNGG 1 cut(s) 364
TaaI ACNGT 2 cut(s) 166, 248
TaqI TCGA 1 cut(s) 384
TasI AATT 6 cut(s) 68, 74, 79, 90, 214, 305
TatI WGTACW 1 cut(s) 483
Tru1I TTAA 4 cut(s) 71, 78, 93, 508
Tru9I TTAA 4 cut(s) 71, 78, 93, 508
TscAI CASTG 3 cut(s) 141, 251, 383
TseFI GTSAC 3 cut(s) 145, 166, 205
TseI GCWGC 2 cut(s) 139, 449
Tsp45I GTSAC 3 cut(s) 145, 166, 205
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 3 cut(s) 141, 251, 383
XapI RAATTY 2 cut(s) 74, 305
XspI CTAG 4 cut(s) 17, 21, 143, 321
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.