Rorug05G0227600

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000577
Physical Location & Seq
Forward (+)
23629183 .. 23631722
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0227600.1

Sequence Viewer

Length: 2073 bp
ATGACACTAAAGTTCTCCCACAAGCAGCTTCAATGCTTCGTTTTTCTTCTCTTGATCTCCTCTGCAACTTCATTGACCTTTGATTTCCCTAGTTTCAGTAGCAATGATACCAACACAGTATTTCTAGAGGGAGACGCTTATATCCGCAACCAATCCATCAACCTCTGCATCATAAACACAACCGGTAGCAGCATTGGTCGAGCCAGTTACAGTGAACCCTTCCTCCTCCGAGAAAACAGCACCGGAAAACTGGCCGACTTCACCACAAACTTCACATTCAACATCGAACTGCCAAACAAAACAAGATATGCAGATGGGATGGTTTTCTTCCTCGCACCAAACGGATCCAAGCTCAACCGCAAACAAGGAGGTGGAGGCAGTCTCGGCCTCCCCTTCGACGACCCCATCACAGCAGCCTCAAAGAATGCGTTCGCCTTTGTCGCCGTGGAGTTCGATATCTATTACAATCCTGAGTATTCCATAGAGGATCCAGCCTACCCTCATCTGGGTATCGACGTCAATACTCTCAAGTCAAACGTGACAGCACCTTGGAATGGAAGTCTTCCGGATGGAAGAAACAATAGTGCTTGGATCAGTTATAGTTCTGGCACCAAAAATCTTAGTGTTTCCTTCACTAATTATGTGAATGGTACCCAAGAGATTAATTACCTCAGTTACACGGTTGATCTCAATCAATACTTACCTGATTCAGTCATTGTTGGGTTCTCCGCTGCCACTGGTGTTCGTATTGCTCTACCCAAGATACATTCATGGAGTTTTAGTTCTTCATTAGTCCTCCCACCGCCAAGAGTCGGTACTAATCCCAAGTCAGGAAAGAACAAAATAGGACTAGCGGTGGGGTTGAGTGTTGGTGGAGGTGCTATCTTGGTAAGCGGGTTAGGGTTGATTTGGTTGTTCATGGGCAAGAAGAGGGGAGGAGGAGAAGGAAGTGATGAGGATCTCGTACTTCGCCACTTCATTGATGAGGAGTTTGAAAGAGGAACAGGTCCACGGAAGTTTTCCTACAATGAGTTGGCCCAAGCGACCAATAATTTTGAGGAGGGAGGGAAGCTTGCAGAGGGAGGGTTTGGTGGTGTATACAGAGGCTTCATAAAAGACTTGAATTCCTATGTTGCTGTCAAGAAGATATCAAGTGGGTCTAAACAAGGACTGAAGGAGTATGCGGCAGAAGTAAAGAGCATAAGTCGACTTAGGCATCGGAATCTAGTGCAACTCATTGGTTGGTGCCACGAGAAGAAACTCCTACTCGTCTACGAACTTATGCCTAATGGTAGCTTAGATTCACATTTGTTCAAAGAAAAAAGCTTGTTACATTGGGATGTACGGTACAAAATTGCACAAGGCTTGGCCTCGGGGCTGCTCTATCTACATGATGAATGGGAACAATGTGTACTGCACAGGGATATTAAATCCAGCAATGTTATGTTGGATTCAAATTTCAAGGCGAAACTTGGGGATTTCGGACTAGCTCGACTTGTGGACCACGGGCAACAATCACAAACAACAATTGTAGCAGGAACCAGGGGATACATGGCTCTCGAATACGTTGTATCAGGAAAGGCGAGCAAGCAATCAGATGTGTACAGCTTCGGAATTGTTGCTTTAGAAATAGCATGCGGGAGAAAACCCATCGATCTAAGTTTGGAAAGTAGTCAAGTCGATATGGTGGAGTGGGTTTGGGAGCTTTATGGTGAAGCGAAAGTCATTGAAGCAGCCGATCCAAAATTGGGTGGAGATTTTGATGAGAAACAAATGGAGTGCTTAATGATTGTTGGATTGTGGTGTGCTCATCCGGATTATAATTTTAGACCTTCAATACAACAAGCGATACAAGTGCTTAACTTCGAAGTTCCACTGCCGAGTCTTCCATTAAATATGCCAGTGTCAACATTTCTCGCTCATCCAGTACCTCCAATGTTCTCCAGTGCTGATAATACATGTTCTGAAATGGGCCAGAATTATGAGTCTTCAGGCAATACTAATAGCATGAATTCCTCAATGGGCACAGTGTCTTCTACCTCAAATTCCCATACATCGGCATCGCTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

690

Amino Acids

75.56

Weight (kDa)

5.92

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 25 - 263 5.7e-59 Legume lectin domain
Lectin_L-type_dom PF18483 42 - 252 5.5e-12 Legume lectin beta-barrel domain
Pkinase PF00069 352 - 618 2.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 356 - 553 9.9e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16960 AT3G16970 AT3G17080 AT4G16195 AT5G12060 AT5G12070
fragaria_vesca FvH4_2g16811 FvH4_4g02030 FvH4_4g02050 FvH4_4g02160 FvH4_4g05620
malus_domestica MD04G1130600.v1.1 MD07G1006200.v1.1 MD07G1270000.v1.1 MD09G1134100.v1.1 MD09G1134200.v1.1 MD10G1085700.v1.1 MD17G1052500.v1.1 MD17G1052600.v1.1 MD17G1052700.v1.1 MD17G1123000.v1.1
prunus_persica Prupe.1G026600_v2.0.a1 Prupe.1G049500_v2.0.a1 Prupe.1G055500_v2.0.a1 Prupe.1G055600_v2.0.a1 Prupe.1G057000_v2.0.a1 Prupe.1G057100_v2.0.a1 Prupe.1G057200_v2.0.a1 Prupe.1G057300_v2.0.a1 Prupe.1G058100_v2.0.a1 Prupe.8G012700_v2.0.a1
pyrus_communis pycom17g05060 pycom17g11360
rosa_chinensis RchiOBHm_Chr3g0496401 RchiOBHm_Chr4g0389011 RchiOBHm_Chr4g0389231 RchiOBHm_Chr4g0389421 RchiOBHm_Chr4g0396771 RchiOBHm_Chr4g0396781 RchiOBHm_Chr4g0396791 RchiOBHm_Chr4g0399631 RchiOBHm_Chr4g0399641 RchiOBHm_Chr5g0071851 RchiOBHm_Chr6g0268511
rosa_laevigata RLG00000009249 RLG00000009256 RLG00000009505 RLG00000009983 RLG00000009984 RLG00000013253
rosa_multiflora Rmu_sc0000487.1_g000011 Rmu_sc0002404.1_g000026
rosa_roxburghii Rroxscaffold_5G00335430 Rroxscaffold_5G00341610
rosa_rugosa Rorug03G0282200 Rorug03G0347600 Rorug04G0017800 Rorug04G0017800 Rorug05G0227600 Rorug06G0042400 Rorug06G0042500
rosa_samantha Rh3AG328900 Rh4AG027600 Rh4BG093100 Rh4CG029700 Rh4CG077600 Rh4CG077800 Rh4CG104100 Rh4CG104200 Rh4DG020800 Rh4DG021100 Rh4DG021200 Rh4DG067000 Rh4DG067100 Rh6AG162000 Rh6BG166300
rosa_wichuraiana Rw4G002000 Rw4G005780 Rw4G005790 Rw4G007880 Rw6G013990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1821
AatII GACGTC 1 cut(s) 519
Acc65I GGTACC 1 cut(s) 650
AccB1I GGYRCC 3 cut(s) 608, 650, 1245
AccI GTMKAC 3 cut(s) 1098, 1207, 1272
AccIII TCCGGA 2 cut(s) 565, 1813
AciI CCGC 8 cut(s) 145, 358, 729, 803, 854, 894, 1184, 1638
AclWI GGATC 7 cut(s) 339, 352, 482, 495, 599, 966, 1733
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 4 cut(s) 1123, 1456, 2011, 2044
AcuI CTGAAG 2 cut(s) 1193, 1974
AcyI GRCGYC 1 cut(s) 516
AfaI GTAC 8 cut(s) 652, 817, 966, 1344, 1349, 1413, 1604, 1929
AfiI CCNNNNNNNGG 7 cut(s) 505, 506, 554, 812, 830, 1748, 2056
AflIII ACRYGT 1 cut(s) 1958
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 9 cut(s) 32, 280, 995, 1123, 1315, 1455, 1462, 1730, 1836
AjnI CCWGG 1 cut(s) 1541
AloI GAACNNNNNNTCC 4 cut(s) 207, 239, 766, 798
AluBI AGCT 8 cut(s) 28, 352, 1072, 1296, 1326, 1490, 1608, 1705
AluI AGCT 8 cut(s) 28, 352, 1072, 1296, 1326, 1490, 1608, 1705
Alw21I GWGCWC 1 cut(s) 1810
Alw26I GTCTC 2 cut(s) 126, 386
AlwI GGATC 7 cut(s) 339, 352, 482, 495, 599, 966, 1733
Ama87I CYCGRG 1 cut(s) 1372
Aor13HI TCCGGA 2 cut(s) 565, 1813
AoxI GGCC 5 cut(s) 252, 385, 1035, 1368, 1972
ApeKI GCWGC 6 cut(s) 25, 189, 413, 731, 1378, 1733
ApoI RAATTY 4 cut(s) 1123, 1456, 2011, 2044
AseI ATTAAT 1 cut(s) 663
AsiGI ACCGGT 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 428
Asp718I GGTACC 1 cut(s) 650
AspS9I GGNCC 4 cut(s) 1007, 1036, 1501, 1972
AsuHPI GGTGA 2 cut(s) 253, 1724
AsuII TTCGAA 1 cut(s) 1866
AvaI CYCGRG 1 cut(s) 1372
AvaII GGWCC 2 cut(s) 1007, 1501
BaeGI GKGCMC 1 cut(s) 2027
BamHI GGATCC 2 cut(s) 344, 487
BanI GGYRCC 3 cut(s) 608, 650, 1245
BarI GAAGNNNNNNTAC 2 cut(s) 1007, 1039
BauI CACGAG 1 cut(s) 1250
BbsI GAAGAC 4 cut(s) 554, 1877, 1980, 2025
Bbv12I GWGCWC 1 cut(s) 1810
BbvI GCAGC 6 cut(s) 37, 201, 425, 718, 1365, 1745
BccI CCATC 6 cut(s) 164, 308, 313, 413, 563, 1658
BceAI ACGGC 1 cut(s) 428
BcgI CGANNNNNNTGC 2 cut(s) 1837, 1871
BciT130I CCWGG 1 cut(s) 1543
BciVI GTATCC 1 cut(s) 1541
BcoDI GTCTC 2 cut(s) 126, 386
BfaI CTAG 5 cut(s) 90, 125, 851, 1226, 1487
BfuI GTATCC 1 cut(s) 1541
BisI GCNGC 7 cut(s) 26, 190, 414, 732, 1185, 1379, 1734
BlsI GCNGC 7 cut(s) 27, 191, 415, 733, 1186, 1380, 1735
Bme1390I CCNGG 1 cut(s) 1543
Bme18I GGWCC 2 cut(s) 1007, 1501
BmeT110I CYCGRG 1 cut(s) 1372
BmgT120I GGNCC 4 cut(s) 1007, 1036, 1501, 1972
BmiI GGNNCC 6 cut(s) 346, 489, 610, 652, 1247, 1540
BmrFI CCNGG 1 cut(s) 1543
BmsI GCATC 3 cut(s) 177, 1225, 2069
BpiI GAAGAC 4 cut(s) 554, 1877, 1980, 2025
BplI GAGNNNNNCTC 2 cut(s) 366, 398
BpmI CTGGAG 1 cut(s) 1927
Bpu14I TTCGAA 1 cut(s) 1866
BpuEI CTTGAG 1 cut(s) 512
Bsa29I ATCGAT 1 cut(s) 1653
BsaBI GATNNNNATC 2 cut(s) 690, 957
BsaHI GRCGYC 1 cut(s) 516
BsaJI CCNNGG 6 cut(s) 444, 548, 1010, 1371, 1504, 1542
BsaWI WCCGGW 4 cut(s) 182, 242, 565, 1813
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 766, 796
Bsc4I CCNNNNNNNGG 7 cut(s) 505, 506, 554, 812, 830, 1748, 2056
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 6 cut(s) 204, 255, 742, 1901, 1925, 1944
Bse3DI GCAATG 2 cut(s) 109, 1444
Bse8I GATNNNNATC 2 cut(s) 690, 957
BseAI TCCGGA 2 cut(s) 565, 1813
BseBI CCWGG 1 cut(s) 1543
BseCI ATCGAT 1 cut(s) 1653
BseDI CCNNGG 6 cut(s) 444, 548, 1010, 1371, 1504, 1542
BseGI GGATG 5 cut(s) 324, 574, 1345, 1810, 1921
BseJI GATNNNNATC 2 cut(s) 690, 957
BseLI CCNNNNNNNGG 7 cut(s) 505, 506, 554, 812, 830, 1748, 2056
BseMI GCAATG 2 cut(s) 109, 1444
BseMII CTCAG 2 cut(s) 462, 685
BseNI ACTGG 6 cut(s) 204, 255, 742, 1901, 1925, 1944
BseRI GAGGAG 6 cut(s) 49, 215, 951, 954, 1001, 1073
BseSI GKGCMC 1 cut(s) 2027
BseXI GCAGC 6 cut(s) 37, 201, 425, 718, 1365, 1745
BsgI GTGCAG 1 cut(s) 1400
BshFI GGCC 5 cut(s) 254, 387, 1037, 1370, 1974
BshNI GGYRCC 3 cut(s) 608, 650, 1245
BshTI ACCGGT 1 cut(s) 182
BshVI ATCGAT 1 cut(s) 1653
BsiHKAI GWGCWC 1 cut(s) 1810
BsiHKCI CYCGRG 1 cut(s) 1372
BsiSI CCGG 4 cut(s) 183, 243, 566, 1814
BslI CCNNNNNNNGG 7 cut(s) 505, 506, 554, 812, 830, 1748, 2056
BsmAI GTCTC 2 cut(s) 126, 386
BsmBI CGTCTC 1 cut(s) 126
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 5 cut(s) 254, 387, 1037, 1370, 1974
BsoBI CYCGRG 1 cut(s) 1372
Bsp119I TTCGAA 1 cut(s) 1866
Bsp1286I GDGCHC 2 cut(s) 1810, 2027
Bsp13I TCCGGA 2 cut(s) 565, 1813
Bsp1407I TGTACA 1 cut(s) 1602
Bsp143I GATC 8 cut(s) 54, 344, 487, 591, 685, 958, 1654, 1738
BspACI CCGC 8 cut(s) 145, 358, 729, 803, 854, 894, 1184, 1638
BspANI GGCC 5 cut(s) 254, 387, 1037, 1370, 1974
BspCNI CTCAG 2 cut(s) 463, 684
BspDI ATCGAT 1 cut(s) 1653
BspEI TCCGGA 2 cut(s) 565, 1813
BspLI GGNNCC 6 cut(s) 346, 489, 610, 652, 1247, 1540
BspPI GGATC 7 cut(s) 339, 352, 482, 495, 599, 966, 1733
BspT104I TTCGAA 1 cut(s) 1866
BspT107I GGYRCC 3 cut(s) 608, 650, 1245
BsrDI GCAATG 2 cut(s) 109, 1444
BsrFI RCCGGY 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 1602
BsrI ACTGG 6 cut(s) 204, 255, 742, 1901, 1925, 1944
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 6 cut(s) 444, 548, 1010, 1371, 1504, 1542
BssMI GATC 8 cut(s) 54, 344, 487, 591, 685, 958, 1654, 1738
BssNAI GTATAC 1 cut(s) 1099
BssNI GRCGYC 1 cut(s) 516
BssSI CACGAG 1 cut(s) 1250
BssT1I CCWWGG 1 cut(s) 548
Bst1107I GTATAC 1 cut(s) 1099
Bst2BI CACGAG 1 cut(s) 1250
Bst2UI CCWGG 1 cut(s) 1543
Bst4CI ACNGT 5 cut(s) 118, 212, 682, 1347, 2029
Bst6I CTCTTC 1 cut(s) 923
BstACI GRCGYC 1 cut(s) 516
BstAUI TGTACA 1 cut(s) 1602
BstBI TTCGAA 1 cut(s) 1866
BstC8I GCNNGC 4 cut(s) 1074, 1585, 1589, 1636
BstDEI CTNAG 6 cut(s) 471, 620, 671, 1211, 1297, 1658
BstDSI CCRYGG 3 cut(s) 444, 1010, 1504
BstF5I GGATG 5 cut(s) 324, 574, 1345, 1810, 1921
BstKTI GATC 8 cut(s) 57, 347, 490, 594, 688, 961, 1657, 1741
BstMAI GTCTC 2 cut(s) 126, 386
BstMBI GATC 8 cut(s) 54, 344, 487, 591, 685, 958, 1654, 1738
BstMWI GCNNNNNNNGC 2 cut(s) 384, 440
BstNI CCWGG 1 cut(s) 1543
BstNSI RCATGY 2 cut(s) 1638, 1962
BstSCI CCNGG 1 cut(s) 1541
BstSLI GKGCMC 1 cut(s) 2027
BstV1I GCAGC 6 cut(s) 37, 201, 425, 718, 1365, 1745
BstV2I GAAGAC 4 cut(s) 554, 1877, 1980, 2025
BstX2I RGATCY 3 cut(s) 344, 487, 958
BstYI RGATCY 3 cut(s) 344, 487, 958
BstZ17I GTATAC 1 cut(s) 1099
Bsu15I ATCGAT 1 cut(s) 1653
BsuI GTATCC 1 cut(s) 1541
BsuRI GGCC 5 cut(s) 254, 387, 1037, 1370, 1974
BsuTUI ATCGAT 1 cut(s) 1653
BtgI CCRYGG 3 cut(s) 444, 1010, 1504
BtgZI GCGATG 1 cut(s) 2046
BtsCI GGATG 5 cut(s) 324, 574, 1345, 1810, 1921
BtsI GCAGTG 1 cut(s) 1874
BtsIMutI CAGTG 6 cut(s) 217, 735, 1874, 1908, 1951, 2034
Cac8I GCNNGC 4 cut(s) 1074, 1585, 1589, 1636
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 4 cut(s) 1007, 1036, 1501, 1972
ClaI ATCGAT 1 cut(s) 1653
CseI GACGC 1 cut(s) 143
Csp6I GTAC 8 cut(s) 651, 816, 965, 1343, 1348, 1412, 1603, 1928
CspAI ACCGGT 1 cut(s) 182
CviAII CATG 7 cut(s) 771, 919, 1391, 1552, 1635, 1959, 2008
CviQI GTAC 8 cut(s) 651, 816, 965, 1343, 1348, 1412, 1603, 1928
DdeI CTNAG 6 cut(s) 471, 620, 671, 1211, 1297, 1658
DpnI GATC 8 cut(s) 56, 346, 489, 593, 687, 960, 1656, 1740
DpnII GATC 8 cut(s) 54, 344, 487, 591, 685, 958, 1654, 1738
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 923
EarI CTCTTC 1 cut(s) 923
Eco130I CCWWGG 1 cut(s) 548
Eco32I GATATC 2 cut(s) 457, 1149
Eco47I GGWCC 2 cut(s) 1007, 1501
Eco57I CTGAAG 2 cut(s) 1193, 1974
Eco88I CYCGRG 1 cut(s) 1372
EcoRI GAATTC 2 cut(s) 1123, 2011
EcoRII CCWGG 1 cut(s) 1541
EcoRV GATATC 2 cut(s) 457, 1149
EcoT14I CCWWGG 1 cut(s) 548
ErhI CCWWGG 1 cut(s) 548
Esp3I CGTCTC 1 cut(s) 126
FaeI CATG 7 cut(s) 774, 922, 1394, 1555, 1638, 1962, 2011
FatI CATG 7 cut(s) 770, 918, 1390, 1551, 1634, 1958, 2007
FauI CCCGC 2 cut(s) 887, 1631
FblI GTMKAC 3 cut(s) 1098, 1207, 1272
Fnu4HI GCNGC 7 cut(s) 26, 190, 414, 732, 1185, 1379, 1734
FokI GGATG 5 cut(s) 331, 581, 1352, 1797, 1908
Fsp4HI GCNGC 7 cut(s) 26, 190, 414, 732, 1185, 1379, 1734
FspBI CTAG 5 cut(s) 90, 125, 851, 1226, 1487
GluI GCNGC 7 cut(s) 26, 190, 414, 732, 1185, 1379, 1734
GsuI CTGGAG 1 cut(s) 1927
HaeIII GGCC 5 cut(s) 254, 387, 1037, 1370, 1974
HapII CCGG 4 cut(s) 183, 243, 566, 1814
HgaI GACGC 1 cut(s) 143
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 7 cut(s) 774, 922, 1394, 1555, 1638, 1962, 2011
HincII GTYRAC 2 cut(s) 1208, 1908
HindII GTYRAC 2 cut(s) 1208, 1908
HindIII AAGCTT 2 cut(s) 1070, 1324
HinfI GANTC 7 cut(s) 707, 810, 1222, 1301, 1451, 1882, 1985
HpaII CCGG 4 cut(s) 183, 243, 566, 1814
HphI GGTGA 2 cut(s) 253, 1724
Hpy166II GTNNAC 9 cut(s) 215, 1010, 1099, 1208, 1273, 1412, 1501, 1603, 1908
Hpy188I TCNGA 6 cut(s) 230, 1221, 1484, 1597, 1613, 1966
Hpy188III TCNNGA 9 cut(s) 52, 125, 470, 566, 831, 1141, 1559, 1575, 1814
Hpy8I GTNNAC 9 cut(s) 215, 1010, 1099, 1208, 1273, 1412, 1501, 1603, 1908
Hpy99I CGWCG 2 cut(s) 401, 518
HpyAV CCTTC 6 cut(s) 229, 403, 640, 938, 1168, 1842
HpyCH4III ACNGT 5 cut(s) 118, 212, 682, 1347, 2029
HpyCH4IV ACGT 3 cut(s) 516, 537, 1566
HpyCH4V TGCA 7 cut(s) 65, 168, 311, 1076, 1231, 1358, 1417
HpyF10VI GCNNNNNNNGC 2 cut(s) 384, 440
HpyF3I CTNAG 6 cut(s) 471, 620, 671, 1211, 1297, 1658
HpySE526I ACGT 3 cut(s) 516, 537, 1566
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 7 cut(s) 774, 922, 1394, 1555, 1638, 1962, 2011
Kpn2I TCCGGA 2 cut(s) 565, 1813
KpnI GGTACC 1 cut(s) 654
Kzo9I GATC 8 cut(s) 54, 344, 487, 591, 685, 958, 1654, 1738
LmnI GCTCC 1 cut(s) 1702
Lsp1109I GCAGC 6 cut(s) 37, 201, 425, 718, 1365, 1745
LweI GCATC 3 cut(s) 177, 1225, 2069
MaeI CTAG 5 cut(s) 90, 125, 851, 1226, 1487
MaeII ACGT 3 cut(s) 516, 537, 1566
MaeIII GTNAC 4 cut(s) 206, 538, 674, 1329
MalI GATC 8 cut(s) 56, 346, 489, 593, 687, 960, 1656, 1740
MboI GATC 8 cut(s) 54, 344, 487, 591, 685, 958, 1654, 1738
MfeI CAATTG 1 cut(s) 1527
MflI RGATCY 3 cut(s) 344, 487, 958
MhlI GDGCHC 2 cut(s) 1810, 2027
MlyI GAGTC 3 cut(s) 819, 1891, 1994
MmeI TCCRAC 2 cut(s) 1428, 1774
MroI TCCGGA 2 cut(s) 565, 1813
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 6 cut(s) 663, 1428, 1784, 1860, 1892, 2071
MslI CAYNNNNRTG 1 cut(s) 1338
MspA1I CMGCKG 1 cut(s) 731
MspI CCGG 4 cut(s) 183, 243, 566, 1814
MspR9I CCNGG 1 cut(s) 1543
MunI CAATTG 1 cut(s) 1527
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 1 cut(s) 1543
MwoI GCNNNNNNNGC 2 cut(s) 384, 440
NdeII GATC 8 cut(s) 54, 344, 487, 591, 685, 958, 1654, 1738
NlaIII CATG 7 cut(s) 774, 922, 1394, 1555, 1638, 1962, 2011
NlaIV GGNNCC 6 cut(s) 346, 489, 610, 652, 1247, 1540
NmeAIII GCCGAG 2 cut(s) 363, 1905
NmuCI GTSAC 1 cut(s) 538
NspI RCATGY 2 cut(s) 1638, 1962
NspV TTCGAA 1 cut(s) 1866
PaeI GCATGC 1 cut(s) 1638
PciI ACATGT 1 cut(s) 1958
PctI GAATGC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 4 cut(s) 707, 1222, 1301, 1451
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 7 cut(s) 27, 191, 415, 733, 1186, 1380, 1735
PleI GAGTC 3 cut(s) 818, 1890, 1993
PpsI GAGTC 3 cut(s) 818, 1890, 1993
PscI ACATGT 1 cut(s) 1958
PshBI ATTAAT 1 cut(s) 663
PsiI TTATAA 1 cut(s) 1821
Psp6I CCWGG 1 cut(s) 1541
PspGI CCWGG 1 cut(s) 1541
PspN4I GGNNCC 6 cut(s) 346, 489, 610, 652, 1247, 1540
PspPI GGNCC 4 cut(s) 1007, 1036, 1501, 1972
PsrI GAACNNNNNNTAC 2 cut(s) 1395, 1427
PsuI RGATCY 3 cut(s) 344, 487, 958
RsaI GTAC 8 cut(s) 652, 817, 966, 1344, 1349, 1413, 1604, 1929
RsaNI GTAC 8 cut(s) 651, 816, 965, 1343, 1348, 1412, 1603, 1928
RseI CAYNNNNRTG 1 cut(s) 1338
SalI GTCGAC 1 cut(s) 1206
SaqAI TTAA 6 cut(s) 663, 1428, 1784, 1860, 1892, 2071
SatI GCNGC 7 cut(s) 26, 190, 414, 732, 1185, 1379, 1734
Sau3AI GATC 8 cut(s) 54, 344, 487, 591, 685, 958, 1654, 1738
Sau96I GGNCC 4 cut(s) 1007, 1036, 1501, 1972
SchI GAGTC 3 cut(s) 819, 1891, 1994
ScrFI CCNGG 1 cut(s) 1543
SduI GDGCHC 2 cut(s) 1810, 2027
SfaNI GCATC 3 cut(s) 177, 1225, 2069
SfuI TTCGAA 1 cut(s) 1866
SinI GGWCC 2 cut(s) 1007, 1501
SmiMI CAYNNNNRTG 1 cut(s) 1338
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
SphI GCATGC 1 cut(s) 1638
SsiI CCGC 8 cut(s) 145, 358, 729, 803, 854, 894, 1184, 1638
SspMI CTAG 5 cut(s) 90, 125, 851, 1226, 1487
StyD4I CCNGG 1 cut(s) 1541
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 5 cut(s) 118, 212, 682, 1347, 2029
TaiI ACGT 3 cut(s) 519, 540, 1569
TatI WGTACW 2 cut(s) 1411, 1602
TauI GCSGC 1 cut(s) 1187
TfiI GAWTC 4 cut(s) 707, 1222, 1301, 1451
Tru1I TTAA 6 cut(s) 663, 1428, 1784, 1860, 1892, 2071
Tru9I TTAA 6 cut(s) 663, 1428, 1784, 1860, 1892, 2071
TscAI CASTG 6 cut(s) 217, 742, 1881, 1908, 1951, 2034
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 6 cut(s) 25, 189, 413, 731, 1378, 1733
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 8 cut(s) 60, 759, 777, 907, 967, 1099, 1410, 2024
TspGWI ACGGA 2 cut(s) 357, 1027
TspRI CASTG 6 cut(s) 217, 742, 1881, 1908, 1951, 2034
VpaK11BI GGWCC 2 cut(s) 1007, 1501
VspI ATTAAT 1 cut(s) 663
XapI RAATTY 4 cut(s) 1123, 1456, 2011, 2044
XbaI TCTAGA 1 cut(s) 124
XceI RCATGY 2 cut(s) 1638, 1962
XcmI CCANNNNNNNNNTGG 1 cut(s) 1549
XmiI GTMKAC 3 cut(s) 1098, 1207, 1272
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 5 cut(s) 90, 125, 851, 1226, 1487
ZraI GACGTC 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.