MD15G1290300.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000218
Physical Location & Seq
Reverse (-)
26827374 .. 26831019
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1290300.v1.1.491

Sequence Viewer

Length: 1512 bp
ATGAAACAACAGATCTTGTTATACATATTCCTCTCCATTATCTCCATCCTCTTCACCTTCAACTTCCTGCTCCAAACAAAACGACGCCGTTACAAAAACCTCCCACCAAGTCCACTTTCTCTTCCACTAATCGGCCATCTCCATCTCCTAAAAAGCCCAATCCATCGAACCCTCCACCGCCTCTCACAAAAGCACGGCCCCATTTTCTCACTCTGGTTTGGCTCGCAGCGCGTGGTCATAGTCTCATCCTCGTCGGCCGCACAAGAATGTTTCACCACCAACGACATCGTTCTAGCAAACCGTCCTAGCTTTATCCTAGGCAAGCACTTAGCCTACAACAACACCACCATGGCAACGTCCCAATACGGCGATCACTGGCGCAACCTCCGCCGCATTGGCGCCATAGAGATATTCTCCACCGCTCGACTGAACACATTTTCGAACATCAGAAAGGACGAAATCAAACAGCTACTACTTAAACTTTCACAAAACGCAAGTGAAGATTTCGCAACGGTGCCGTTGAAGTCTATGTTCCATGAGTTGACCTTTAACACTATAATGAGGATGGTGGCGGGGAAGAGGTACTACGGCGAGGATGTGTCGGTGGAGAAAGAGCAGGCGAGGCAGTTCAGAGAGATTATGAAGGAGGCTTTTGCTCAAAGTGGAGCGGCCAACCCGGCAGATTTTTTGCCGATCTTGAATTGGGTTGGAAGCGACTCGTATGAGAGGAGGGCCATGAAGTTGGGCAAGAGGACCAATAAGTTTTTCCAAGGCCTCATCGATGAGCACAGGAGCAAGAAGGGTGGAACTACTAGTACTATGATTGACCATTTGCTTACTCTGCAGGGATCACAACCTGAGTATTACACTGACCAAATTATCAAGGGGATTATACAGGTCATAATAATGGCGGGTACTGATACATCAGCAGTGACACTAGAATGGGCCATGTCCAATCTCCTCAACCATCCCCTCGTGTTAAAGAAAGCTAGAGCTGAACTTGATGCTCAACTTGGAAATGAACGCTTGGTGGATGAACCGGATATCTCTAAACTACCCTATCTGCAAAGTATCATCTCCGAGACCCTCCGATTATACCCTGCAGCTCCATTGCTACTACCACATTTTTCATCAGATGACTGCATGATTGAGAGATTCAACGTGCCACGTGACACAATGGTATTGATCAATGCATGGGCCATCCATAGAGACCCAATGTTGTGGGATGATCCTGAAAGCTTCAAACCCGAGAGATTTGAGAATGGTGAGGACTCGTCACACAACCTGATGCCGTTCGGACTGGGAAGAAGGGCTTGCCCTGGGGAGGGCCTAGCGAAACGTATGGTGGGGTTGACTTTGGGGTCGTTGATCCAATGCTTTGAGTGGAAGAGAGTTAATGAAAAGGAGATTGATATGGCAGAAGGTAAGGGATTCACAATGCCTAAAGCCGTGCCATTGGAAGCTAAGTGCAAAGCACGCTCTGTTATTGTGAACGAGATTATGATGGCTTGA

Protein Analysis

504

Amino Acids

57.21

Weight (kDa)

9.22

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 482 2.9e-95 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66540 AT1G66540 AT4G37340 AT4G37360 AT4G37370 AT5G36220 AT5G36220
fragaria_vesca FvH4_1g16862 FvH4_1g16870 FvH4_1g16871 FvH4_1g16920 FvH4_1g16921 FvH4_1g16930 FvH4_1g23681 FvH4_1g23710
malus_domestica MD03G1281500.v1.1 MD08G1089700.v1.1 MD15G1074700.v1.1 MD15G1289800.v1.1 MD15G1290300.v1.1
prunus_persica Prupe.6G226600_v2.0.a1 Prupe.6G226800_v2.0.a1 Prupe.6G226900_v2.0.a1 Prupe.6G227000_v2.0.a1 Prupe.6G227100_v2.0.a1 Prupe.6G227300_v2.0.a1 Prupe.6G227400_v2.0.a1
pyrus_communis pycom15g25350 pycom15g25360 pycom15g25370 pycom15g25400
rosa_chinensis RchiOBHm_Chr2g0106831 RchiOBHm_Chr2g0106851 RchiOBHm_Chr2g0106901 RchiOBHm_Chr2g0106911 RchiOBHm_Chr2g0106971 RchiOBHm_Chr2g0106991 RchiOBHm_Chr2g0119331 RchiOBHm_Chr2g0119351 RchiOBHm_Chr2g0119391 RchiOBHm_Chr2g0119411 RchiOBHm_Chr2g0119481 RchiOBHm_Chr2g0119541 RchiOBHm_Chr5g0076421
rosa_laevigata RLG00000012204 RLG00000017542 RLG00000017543 RLG00000017547 RLG00000017549 RLG00000018145 RLG00000018146 RLG00000018148 RLG00000018462 RLG00000018464 RLG00000018465 RLG00000018468 RLG00000018470 RLG00000018471 RLG00000018539 RLG00000018540 RLG00000018592
rosa_multiflora Rmu_co8030410.1_g000001 Rmu_co8333451.1_g000001 Rmu_co8389363.1_g000001 Rmu_sc0001801.1_g000006 Rmu_sc0001801.1_g000023 Rmu_sc0001801.1_g000036 Rmu_sc0002788.1_g000013 Rmu_sc0002868.1_g000014 Rmu_sc0004344.1_g000013 Rmu_sc0004344.1_g000021 Rmu_sc0004344.1_g000027 Rmu_sc0004656.1_g000001 Rmu_sc0006098.1_g000002 Rmu_sc0006098.1_g000007 Rmu_sc0007106.1_g000005 Rmu_sc0007106.1_g000009 Rmu_sc0010912.1_g000001 Rmu_sc0021576.1_g000001 Rmu_sc0031470.1_g000001
rosa_roxburghii Rroxscaffold_2G00122810 Rroxscaffold_2G00123600 Rroxscaffold_2G00124270 Rroxscaffold_2G00124320 Rroxscaffold_2G00124350 Rroxscaffold_2G00136690 Rroxscaffold_2G00136740 Rroxscaffold_2G00136750 Rroxscaffold_2G00136760
rosa_rugosa Rorug02G0139000 Rorug02G0139100 Rorug02G0139200 Rorug02G0139300 Rorug02G0139700 Rorug02G0139900 Rorug02G0221400 Rorug02G0221900 Rorug02G0222100 Rorug02G0231700 Rorug02G0231800 Rorug02G0238400 Rorug02G0238500 Rorug05G0413800 Rorug06G0369500 Rorug07G0161900 Rorug07G0309500.1
rosa_samantha Rh2AG190700 Rh2AG279600 Rh2BG201600 Rh2BG201900 Rh2BG202200 Rh2BG288600 Rh2BG290400 Rh2BG290600 Rh2BG291000 Rh2BG291100 Rh2BG291200 Rh2BG291300 Rh2CG195400 Rh2DG196900 Rh2DG197400 Rh2DG197700 Rh2DG283900 Rh2DG285600 Rh2DG285700 Rh2DG286000 Rh2DG286100 Rh2DG286200 Rh2DG286300 Rh2DG305000 Rh2DG305100 Rh2DG305200 Rh2DG305400 Rh5DG500100 Rh6AG481700 Rh6BG491200 Rh6DG482200
rosa_wichuraiana Rw2G014970 Rw2G015010 Rw2G015030 Rw2G022240 Rw2G022250 Rw2G022290 Rw2G022300 Rw2G022310 Rw2G022320 Rw5G034260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1360
AccB1I GGYRCC 2 cut(s) 398, 514
AccBSI CCGCTC 2 cut(s) 422, 668
AccII CGCG 1 cut(s) 231
AciI CCGC 8 cut(s) 178, 258, 388, 391, 420, 572, 668, 911
AclWI GGATC 3 cut(s) 856, 1223, 1363
AcoI YGGCCR 3 cut(s) 133, 255, 669
AcvI CACGTG 1 cut(s) 1169
AcyI GRCGYC 2 cut(s) 85, 399
AfaI GTAC 3 cut(s) 584, 817, 916
AfiI CCNNNNNNNGG 3 cut(s) 131, 1324, 1325
AgsI TTSAA 5 cut(s) 61, 523, 700, 1159, 1243
AhlI ACTAGT 1 cut(s) 812
AjnI CCWGG 1 cut(s) 1318
AjuI GAANNNNNNNTTGG 4 cut(s) 100, 132, 749, 781
AluBI AGCT 7 cut(s) 309, 469, 989, 995, 1106, 1239, 1463
AluI AGCT 7 cut(s) 309, 469, 989, 995, 1106, 1239, 1463
Alw21I GWGCWC 1 cut(s) 789
Alw26I GTCTC 3 cut(s) 247, 1076, 1203
AlwI GGATC 3 cut(s) 856, 1223, 1363
Ama87I CYCGRG 1 cut(s) 1247
AoxI GGCC 9 cut(s) 133, 196, 255, 669, 732, 772, 945, 1197, 1327
ApeKI GCWGC 2 cut(s) 226, 1103
AspA2I CCTAGG 1 cut(s) 316
AspLEI GCGC 3 cut(s) 231, 381, 401
AspS9I GGNCC 6 cut(s) 197, 732, 753, 945, 1197, 1327
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 3 cut(s) 46, 265, 1277
AsuII TTCGAA 1 cut(s) 440
AvaI CYCGRG 1 cut(s) 1247
AvaII GGWCC 1 cut(s) 753
AvrII CCTAGG 1 cut(s) 316
BanI GGYRCC 2 cut(s) 398, 514
BarI GAAGNNNNNNTAC 2 cut(s) 569, 601
BauI CACGAG 1 cut(s) 974
BbrPI CACGTG 1 cut(s) 1169
Bbv12I GWGCWC 1 cut(s) 789
BbvI GCAGC 2 cut(s) 238, 1115
BccI CCATC 8 cut(s) 53, 144, 150, 171, 559, 975, 1208, 1498
BceAI ACGGC 7 cut(s) 72, 211, 382, 502, 604, 1276, 1433
BciT130I CCWGG 1 cut(s) 1320
BclI TGATCA 1 cut(s) 1185
BcnI CCSGG 1 cut(s) 677
BcoDI GTCTC 3 cut(s) 247, 1076, 1203
BcuI ACTAGT 1 cut(s) 812
BfaI CTAG 7 cut(s) 293, 306, 317, 813, 938, 990, 1331
BfmI CTRYAG 2 cut(s) 842, 1101
BfoI RGCGCY 1 cut(s) 402
BglI GCCNNNNNGGC 2 cut(s) 396, 677
BglII AGATCT 1 cut(s) 12
BisI GCNGC 5 cut(s) 227, 258, 391, 669, 1104
BlnI CCTAGG 1 cut(s) 316
BlsI GCNGC 5 cut(s) 228, 259, 392, 670, 1105
BmcAI AGTACT 1 cut(s) 817
Bme1390I CCNGG 2 cut(s) 677, 1320
Bme18I GGWCC 1 cut(s) 753
BmeT110I CYCGRG 1 cut(s) 1247
BmgT120I GGNCC 6 cut(s) 197, 732, 753, 945, 1197, 1327
BmiI GGNNCC 3 cut(s) 199, 400, 516
BmrFI CCNGG 2 cut(s) 677, 1320
BmrI ACTGGG 1 cut(s) 1310
BmsI GCATC 2 cut(s) 994, 1278
BmuI ACTGGG 1 cut(s) 1310
BplI GAGNNNNNCTC 2 cut(s) 398, 430
Bpu14I TTCGAA 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 677
Bsa29I ATCGAT 1 cut(s) 780
BsaAI YACGTR 1 cut(s) 1169
BsaHI GRCGYC 2 cut(s) 85, 399
BsaI GGTCTC 2 cut(s) 1076, 1203
BsaJI CCNNGG 5 cut(s) 316, 348, 769, 1318, 1319
BsaWI WCCGGW 1 cut(s) 1039
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 1324, 1325
Bse1I ACTGG 2 cut(s) 380, 1305
Bse3DI GCAATG 1 cut(s) 1109
BseBI CCWGG 1 cut(s) 1320
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 5 cut(s) 316, 348, 769, 1318, 1319
BseGI GGATG 8 cut(s) 45, 245, 570, 601, 967, 1039, 1200, 1231
BseLI CCNNNNNNNGG 3 cut(s) 131, 1324, 1325
BseMI GCAATG 1 cut(s) 1109
BseMII CTCAG 1 cut(s) 849
BseNI ACTGG 2 cut(s) 380, 1305
BseRI GAGGAG 2 cut(s) 742, 950
BseX3I CGGCCG 1 cut(s) 255
BseXI GCAGC 2 cut(s) 238, 1115
Bsh1236I CGCG 1 cut(s) 231
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 9 cut(s) 135, 198, 257, 671, 734, 774, 947, 1199, 1329
BshNI GGYRCC 2 cut(s) 398, 514
BshVI ATCGAT 1 cut(s) 780
BsiEI CGRYCG 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 789
BsiHKCI CYCGRG 1 cut(s) 1247
BsiSI CCGG 2 cut(s) 677, 1040
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 3 cut(s) 131, 1324, 1325
BsmAI GTCTC 3 cut(s) 247, 1076, 1203
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 9 cut(s) 135, 198, 257, 671, 734, 774, 947, 1199, 1329
Bso31I GGTCTC 2 cut(s) 1076, 1203
BsoBI CYCGRG 1 cut(s) 1247
Bsp119I TTCGAA 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 789
Bsp143I GATC 7 cut(s) 12, 370, 693, 848, 1185, 1228, 1368
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 8 cut(s) 178, 258, 388, 391, 420, 572, 668, 911
BspANI GGCC 9 cut(s) 135, 198, 257, 671, 734, 774, 947, 1199, 1329
BspCNI CTCAG 1 cut(s) 850
BspDI ATCGAT 1 cut(s) 780
BspFNI CGCG 1 cut(s) 231
BspLI GGNNCC 3 cut(s) 199, 400, 516
BspMAI CTGCAG 2 cut(s) 846, 1105
BspPI GGATC 3 cut(s) 856, 1223, 1363
BspT104I TTCGAA 1 cut(s) 440
BspT107I GGYRCC 2 cut(s) 398, 514
BspTNI GGTCTC 2 cut(s) 1076, 1203
BsrBI CCGCTC 2 cut(s) 422, 668
BsrDI GCAATG 1 cut(s) 1109
BsrI ACTGG 2 cut(s) 380, 1305
BssECI CCNNGG 5 cut(s) 316, 348, 769, 1318, 1319
BssMI GATC 7 cut(s) 12, 370, 693, 848, 1185, 1228, 1368
BssNI GRCGYC 2 cut(s) 85, 399
BssSI CACGAG 1 cut(s) 974
BssT1I CCWWGG 3 cut(s) 316, 348, 769
Bst2BI CACGAG 1 cut(s) 974
Bst2UI CCWGG 1 cut(s) 1320
Bst4CI ACNGT 2 cut(s) 302, 514
Bst6I CTCTTC 4 cut(s) 56, 126, 572, 1382
BstACI GRCGYC 2 cut(s) 85, 399
BstBAI YACGTR 1 cut(s) 1169
BstBI TTCGAA 1 cut(s) 440
BstC8I GCNNGC 5 cut(s) 224, 323, 618, 1315, 1477
BstDEI CTNAG 3 cut(s) 328, 858, 1464
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 8 cut(s) 45, 245, 570, 601, 967, 1039, 1200, 1231
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 3 cut(s) 231, 381, 401
BstKTI GATC 7 cut(s) 15, 373, 696, 851, 1188, 1231, 1371
BstMAI GTCTC 3 cut(s) 247, 1076, 1203
BstMBI GATC 7 cut(s) 12, 370, 693, 848, 1185, 1228, 1368
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 7 cut(s) 228, 387, 396, 622, 677, 841, 1476
BstNI CCWGG 1 cut(s) 1320
BstSCI CCNGG 2 cut(s) 675, 1318
BstSFI CTRYAG 2 cut(s) 842, 1101
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 238, 1115
BstX2I RGATCY 1 cut(s) 12
BstXI CCANNNNNNTGG 2 cut(s) 742, 1221
BstYI RGATCY 1 cut(s) 12
BstZI CGGCCG 1 cut(s) 255
Bsu15I ATCGAT 1 cut(s) 780
BsuRI GGCC 9 cut(s) 135, 198, 257, 671, 734, 774, 947, 1199, 1329
BsuTUI ATCGAT 1 cut(s) 780
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 8 cut(s) 45, 245, 570, 601, 967, 1039, 1200, 1231
BtsI GCAGTG 1 cut(s) 936
BtsIMutI CAGTG 3 cut(s) 373, 867, 936
Cac8I GCNNGC 5 cut(s) 224, 323, 618, 1315, 1477
CfoI GCGC 3 cut(s) 231, 381, 401
Cfr13I GGNCC 6 cut(s) 197, 732, 753, 945, 1197, 1327
ClaI ATCGAT 1 cut(s) 780
CseI GACGC 1 cut(s) 93
Csp6I GTAC 3 cut(s) 583, 816, 915
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviAII CATG 6 cut(s) 349, 536, 736, 949, 1144, 1194
CviQI GTAC 3 cut(s) 583, 816, 915
DdeI CTNAG 3 cut(s) 328, 858, 1464
DinI GGCGCC 1 cut(s) 400
DpnI GATC 7 cut(s) 14, 372, 695, 850, 1187, 1230, 1370
DpnII GATC 7 cut(s) 12, 370, 693, 848, 1185, 1228, 1368
DrdI GACNNNNNNGTC 1 cut(s) 1360
DseDI GACNNNNNNGTC 1 cut(s) 1360
EaeI YGGCCR 3 cut(s) 133, 255, 669
EagI CGGCCG 1 cut(s) 255
Eam1104I CTCTTC 4 cut(s) 56, 126, 572, 1382
EarI CTCTTC 4 cut(s) 56, 126, 572, 1382
EciI GGCGGA 1 cut(s) 377
EclXI CGGCCG 1 cut(s) 255
Eco130I CCWWGG 3 cut(s) 316, 348, 769
Eco147I AGGCCT 1 cut(s) 774
Eco31I GGTCTC 2 cut(s) 1076, 1203
Eco32I GATATC 1 cut(s) 1045
Eco47I GGWCC 1 cut(s) 753
Eco52I CGGCCG 1 cut(s) 255
Eco72I CACGTG 1 cut(s) 1169
Eco88I CYCGRG 1 cut(s) 1247
EcoO109I RGGNCCY 1 cut(s) 1327
EcoRII CCWGG 1 cut(s) 1318
EcoRV GATATC 1 cut(s) 1045
EcoT14I CCWWGG 3 cut(s) 316, 348, 769
EcoT22I ATGCAT 1 cut(s) 1195
EgeI GGCGCC 1 cut(s) 400
EheI GGCGCC 1 cut(s) 400
ErhI CCWWGG 3 cut(s) 316, 348, 769
FaeI CATG 6 cut(s) 352, 539, 739, 952, 1147, 1197
FalI AAGNNNNNCTT 2 cut(s) 1297, 1329
FaqI GGGAC 1 cut(s) 343
FatI CATG 6 cut(s) 348, 535, 735, 948, 1143, 1193
FauI CCCGC 2 cut(s) 565, 904
FbaI TGATCA 1 cut(s) 1185
Fnu4HI GCNGC 5 cut(s) 227, 258, 391, 669, 1104
FokI GGATG 8 cut(s) 32, 232, 577, 608, 954, 1046, 1187, 1238
Fsp4HI GCNGC 5 cut(s) 227, 258, 391, 669, 1104
FspBI CTAG 7 cut(s) 293, 306, 317, 813, 938, 990, 1331
GlaI GCGC 3 cut(s) 230, 380, 400
GluI GCNGC 5 cut(s) 227, 258, 391, 669, 1104
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 9 cut(s) 135, 198, 257, 671, 734, 774, 947, 1199, 1329
HapII CCGG 2 cut(s) 677, 1040
HgaI GACGC 1 cut(s) 93
HhaI GCGC 3 cut(s) 231, 381, 401
Hin1I GRCGYC 2 cut(s) 85, 399
Hin1II CATG 6 cut(s) 352, 539, 739, 952, 1147, 1197
Hin6I GCGC 3 cut(s) 229, 379, 399
HinP1I GCGC 3 cut(s) 229, 379, 399
HincII GTYRAC 2 cut(s) 543, 1353
HindII GTYRAC 2 cut(s) 543, 1353
HindIII AAGCTT 1 cut(s) 1237
HinfI GANTC 4 cut(s) 716, 1155, 1271, 1431
HpaII CCGG 2 cut(s) 677, 1040
HphI GGTGA 3 cut(s) 46, 265, 1277
Hpy166II GTNNAC 4 cut(s) 113, 543, 1353, 1492
Hpy188I TCNGA 6 cut(s) 449, 632, 1081, 1091, 1135, 1298
Hpy188III TCNNGA 2 cut(s) 697, 1232
Hpy8I GTNNAC 4 cut(s) 113, 543, 1353, 1492
Hpy99I CGWCG 2 cut(s) 87, 256
HpyAV CCTTC 5 cut(s) 67, 637, 793, 1302, 1415
HpyCH4III ACNGT 2 cut(s) 302, 514
HpyCH4IV ACGT 4 cut(s) 356, 1161, 1168, 1339
HpyCH4V TGCA 6 cut(s) 844, 1066, 1103, 1143, 1193, 1470
HpyF10VI GCNNNNNNNGC 7 cut(s) 228, 387, 396, 622, 677, 841, 1476
HpyF3I CTNAG 3 cut(s) 328, 858, 1464
HpySE526I ACGT 4 cut(s) 356, 1161, 1168, 1339
Hsp92I GRCGYC 2 cut(s) 85, 399
Hsp92II CATG 6 cut(s) 352, 539, 739, 952, 1147, 1197
HspAI GCGC 3 cut(s) 229, 379, 399
KasI GGCGCC 1 cut(s) 398
Ksp22I TGATCA 1 cut(s) 1185
Kzo9I GATC 7 cut(s) 12, 370, 693, 848, 1185, 1228, 1368
LmnI GCTCC 4 cut(s) 75, 665, 792, 1111
Lsp1109I GCAGC 2 cut(s) 238, 1115
LweI GCATC 2 cut(s) 994, 1278
MaeI CTAG 7 cut(s) 293, 306, 317, 813, 938, 990, 1331
MaeII ACGT 4 cut(s) 356, 1161, 1168, 1339
MaeIII GTNAC 4 cut(s) 89, 931, 1169, 1275
MalI GATC 7 cut(s) 14, 372, 695, 850, 1187, 1230, 1370
MbiI CCGCTC 2 cut(s) 422, 668
MboI GATC 7 cut(s) 12, 370, 693, 848, 1185, 1228, 1368
MboII GAAGA 6 cut(s) 43, 113, 512, 589, 1317, 1399
MflI RGATCY 1 cut(s) 12
MhlI GDGCHC 1 cut(s) 789
MluCI AATT 2 cut(s) 700, 876
Mly113I GGCGCC 1 cut(s) 399
MlyI GAGTC 2 cut(s) 710, 1265
MmeI TCCRAC 1 cut(s) 688
Mph1103I ATGCAT 1 cut(s) 1195
MseI TTAA 4 cut(s) 477, 549, 980, 1395
MslI CAYNNNNRTG 5 cut(s) 265, 347, 557, 905, 940
MspI CCGG 2 cut(s) 677, 1040
MspR9I CCNGG 2 cut(s) 677, 1320
MvaI CCWGG 1 cut(s) 1320
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 7 cut(s) 228, 387, 396, 622, 677, 841, 1476
NarI GGCGCC 1 cut(s) 399
NciI CCSGG 1 cut(s) 677
NcoI CCATGG 1 cut(s) 348
NdeII GATC 7 cut(s) 12, 370, 693, 848, 1185, 1228, 1368
NlaIII CATG 6 cut(s) 352, 539, 739, 952, 1147, 1197
NlaIV GGNNCC 3 cut(s) 199, 400, 516
NmuCI GTSAC 3 cut(s) 931, 1169, 1275
NsiI ATGCAT 1 cut(s) 1195
NspV TTCGAA 1 cut(s) 440
PasI CCCWGGG 1 cut(s) 1319
PceI AGGCCT 1 cut(s) 774
PfeI GAWTC 2 cut(s) 1155, 1431
PkrI GCNGC 5 cut(s) 228, 259, 392, 670, 1105
PleI GAGTC 2 cut(s) 710, 1265
PluTI GGCGCC 1 cut(s) 402
PmaCI CACGTG 1 cut(s) 1169
PmlI CACGTG 1 cut(s) 1169
PpsI GAGTC 2 cut(s) 710, 1265
Ppu21I YACGTR 1 cut(s) 1169
Psp6I CCWGG 1 cut(s) 1318
PspCI CACGTG 1 cut(s) 1169
PspGI CCWGG 1 cut(s) 1318
PspN4I GGNNCC 3 cut(s) 199, 400, 516
PspPI GGNCC 6 cut(s) 197, 732, 753, 945, 1197, 1327
PsrI GAACNNNNNNTAC 2 cut(s) 799, 831
PstI CTGCAG 2 cut(s) 846, 1105
PsuI RGATCY 1 cut(s) 12
RsaI GTAC 3 cut(s) 584, 817, 916
RsaNI GTAC 3 cut(s) 583, 816, 915
RseI CAYNNNNRTG 5 cut(s) 265, 347, 557, 905, 940
SaqAI TTAA 4 cut(s) 477, 549, 980, 1395
SatI GCNGC 5 cut(s) 227, 258, 391, 669, 1104
Sau3AI GATC 7 cut(s) 12, 370, 693, 848, 1185, 1228, 1368
Sau96I GGNCC 6 cut(s) 197, 732, 753, 945, 1197, 1327
ScaI AGTACT 1 cut(s) 817
SchI GAGTC 2 cut(s) 710, 1265
ScrFI CCNGG 2 cut(s) 677, 1320
SduI GDGCHC 1 cut(s) 789
SfaNI GCATC 2 cut(s) 994, 1278
SfcI CTRYAG 2 cut(s) 842, 1101
SfoI GGCGCC 1 cut(s) 400
SfuI TTCGAA 1 cut(s) 440
SinI GGWCC 1 cut(s) 753
SmiMI CAYNNNNRTG 5 cut(s) 265, 347, 557, 905, 940
SpeI ACTAGT 1 cut(s) 812
Sse9I AATT 2 cut(s) 700, 876
SseBI AGGCCT 1 cut(s) 774
SsiI CCGC 8 cut(s) 178, 258, 388, 391, 420, 572, 668, 911
SspDI GGCGCC 1 cut(s) 398
SspMI CTAG 7 cut(s) 293, 306, 317, 813, 938, 990, 1331
StuI AGGCCT 1 cut(s) 774
StyD4I CCNGG 2 cut(s) 675, 1318
StyI CCWWGG 3 cut(s) 316, 348, 769
TaaI ACNGT 2 cut(s) 302, 514
TaiI ACGT 4 cut(s) 359, 1164, 1171, 1342
TaqI TCGA 4 cut(s) 166, 424, 440, 780
TasI AATT 2 cut(s) 700, 876
TatI WGTACW 1 cut(s) 815
TauI GCSGC 3 cut(s) 260, 393, 671
TfiI GAWTC 2 cut(s) 1155, 1431
Tru1I TTAA 4 cut(s) 477, 549, 980, 1395
Tru9I TTAA 4 cut(s) 477, 549, 980, 1395
TscAI CASTG 3 cut(s) 380, 874, 936
TseFI GTSAC 3 cut(s) 931, 1169, 1275
TseI GCWGC 2 cut(s) 226, 1103
Tsp45I GTSAC 3 cut(s) 931, 1169, 1275
TspDTI ATGAA 7 cut(s) 17, 656, 752, 1035, 1050, 1119, 1413
TspRI CASTG 3 cut(s) 380, 874, 936
VpaK11BI GGWCC 1 cut(s) 753
XmaJI CCTAGG 1 cut(s) 316
XspI CTAG 7 cut(s) 293, 306, 317, 813, 938, 990, 1331
ZrmI AGTACT 1 cut(s) 817
Zsp2I ATGCAT 1 cut(s) 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.