Rroxscaffold_2G00136740

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000218
Physical Location & Seq
Forward (+)
74416466 .. 74431822
15357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136740.1

Sequence Viewer

Length: 702 bp
ATGCTTTTGGCGGGTACTGATTCGTCATCCTTGACACTGGAATGGGCCATGTCCAATCTGCTCAACCATCCTGAGGTGCTAAAAAGGGCTAGAGCTGAACTGGATGCTAAATTTGGACAAGAACGCTTAATAGATGAACCAGATATCTTCGAATTACCTTACCTACAAGGTATCATCTCTGAGACTCTCCGCTTGTACCCTACAGCGCCACTACTAGTACCACATTATACATCTGATGACTGCACCATTGGAGGATTCGACGTGCCATGTGACACGATAGTAATGATCAACGCGTGGGCCATACACAGAGATCCAACATTGTGGGATGATCCTGAAAGCTTCAAGCCTGAGAGGTTTGCAAATGGTGGAGAAGAGCCATACAAGCTCATGCCATTTGGACAAGGAAGAAGAGCTTGCCCTGGTATGGGCTTGGCCCAACGTGTGGTTGGCTTAACTTTGGGATCATTGATACAATGTTTCGAGTGGGAAAAAGTCAGTGAGATGAAGGTTGATATGAGTGAAGGTAAAGGACTCACAATGCCTAAAGCTGTGCCACTGGAGGCTATGTGCAAAGCCCGCCCAATTGTGAACAAGGTGAGGAGCGAGCCGAGCTTTTCCATATTTTCGATGTGGGACGATATGCTTCGGTCCCCGGCCTGGAGCTTCGATACTTGTCTTGGCGCGGCGCGTGGCGGCGAGTAG

Protein Analysis

233

Amino Acids

25.96

Weight (kDa)

4.88

Isoelectric Point (pI)

51.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 182 4e-62 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66540 AT1G66540 AT4G37340 AT4G37360 AT4G37370 AT5G36220 AT5G36220
fragaria_vesca FvH4_1g16862 FvH4_1g16870 FvH4_1g16871 FvH4_1g16920 FvH4_1g16921 FvH4_1g16930 FvH4_1g23681 FvH4_1g23710
malus_domestica MD03G1281500.v1.1 MD08G1089700.v1.1 MD15G1074700.v1.1 MD15G1289800.v1.1 MD15G1290300.v1.1
prunus_persica Prupe.6G226600_v2.0.a1 Prupe.6G226800_v2.0.a1 Prupe.6G226900_v2.0.a1 Prupe.6G227000_v2.0.a1 Prupe.6G227100_v2.0.a1 Prupe.6G227300_v2.0.a1 Prupe.6G227400_v2.0.a1
pyrus_communis pycom15g25350 pycom15g25360 pycom15g25370 pycom15g25400
rosa_chinensis RchiOBHm_Chr2g0106831 RchiOBHm_Chr2g0106851 RchiOBHm_Chr2g0106901 RchiOBHm_Chr2g0106911 RchiOBHm_Chr2g0106971 RchiOBHm_Chr2g0106991 RchiOBHm_Chr2g0119331 RchiOBHm_Chr2g0119351 RchiOBHm_Chr2g0119391 RchiOBHm_Chr2g0119411 RchiOBHm_Chr2g0119481 RchiOBHm_Chr2g0119541 RchiOBHm_Chr5g0076421
rosa_laevigata RLG00000012204 RLG00000017542 RLG00000017543 RLG00000017547 RLG00000017549 RLG00000018145 RLG00000018146 RLG00000018148 RLG00000018462 RLG00000018464 RLG00000018465 RLG00000018468 RLG00000018470 RLG00000018471 RLG00000018539 RLG00000018540 RLG00000018592
rosa_multiflora Rmu_co8030410.1_g000001 Rmu_co8333451.1_g000001 Rmu_co8389363.1_g000001 Rmu_sc0001801.1_g000006 Rmu_sc0001801.1_g000023 Rmu_sc0001801.1_g000036 Rmu_sc0002788.1_g000013 Rmu_sc0002868.1_g000014 Rmu_sc0004344.1_g000013 Rmu_sc0004344.1_g000021 Rmu_sc0004344.1_g000027 Rmu_sc0004656.1_g000001 Rmu_sc0006098.1_g000002 Rmu_sc0006098.1_g000007 Rmu_sc0007106.1_g000005 Rmu_sc0007106.1_g000009 Rmu_sc0010912.1_g000001 Rmu_sc0021576.1_g000001 Rmu_sc0031470.1_g000001
rosa_roxburghii Rroxscaffold_2G00122810 Rroxscaffold_2G00123600 Rroxscaffold_2G00124270 Rroxscaffold_2G00124320 Rroxscaffold_2G00124350 Rroxscaffold_2G00136690 Rroxscaffold_2G00136740 Rroxscaffold_2G00136750 Rroxscaffold_2G00136760
rosa_rugosa Rorug02G0139000 Rorug02G0139100 Rorug02G0139200 Rorug02G0139300 Rorug02G0139700 Rorug02G0139900 Rorug02G0221400 Rorug02G0221900 Rorug02G0222100 Rorug02G0231700 Rorug02G0231800 Rorug02G0238400 Rorug02G0238500 Rorug05G0413800 Rorug06G0369500 Rorug07G0161900 Rorug07G0309500.1
rosa_samantha Rh2AG190700 Rh2AG279600 Rh2BG201600 Rh2BG201900 Rh2BG202200 Rh2BG288600 Rh2BG290400 Rh2BG290600 Rh2BG291000 Rh2BG291100 Rh2BG291200 Rh2BG291300 Rh2CG195400 Rh2DG196900 Rh2DG197400 Rh2DG197700 Rh2DG283900 Rh2DG285600 Rh2DG285700 Rh2DG286000 Rh2DG286100 Rh2DG286200 Rh2DG286300 Rh2DG305000 Rh2DG305100 Rh2DG305200 Rh2DG305400 Rh5DG500100 Rh6AG481700 Rh6BG491200 Rh6DG482200
rosa_wichuraiana Rw2G014970 Rw2G015010 Rw2G015030 Rw2G022240 Rw2G022250 Rw2G022290 Rw2G022300 Rw2G022310 Rw2G022320 Rw5G034260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 442
AccII CGCG 3 cut(s) 293, 683, 688
AciI CCGC 5 cut(s) 11, 190, 577, 683, 693
AclWI GGATC 3 cut(s) 305, 323, 469
AcsI RAATTY 1 cut(s) 110
AfaI GTAC 3 cut(s) 16, 197, 219
AfiI CCNNNNNNNGG 5 cut(s) 73, 424, 425, 442, 657
AflIII ACRYGT 2 cut(s) 291, 439
AgsI TTSAA 1 cut(s) 343
AhlI ACTAGT 1 cut(s) 214
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 2 cut(s) 418, 656
AluBI AGCT 7 cut(s) 95, 339, 385, 413, 548, 612, 663
AluI AGCT 7 cut(s) 95, 339, 385, 413, 548, 612, 663
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 3 cut(s) 305, 323, 469
AoxI GGCC 4 cut(s) 45, 297, 432, 654
ApoI RAATTY 1 cut(s) 110
AspLEI GCGC 3 cut(s) 208, 683, 688
AspS9I GGNCC 4 cut(s) 45, 297, 433, 648
AsuC2I CCSGG 1 cut(s) 653
AsuHPI GGTGA 1 cut(s) 607
AsuII TTCGAA 1 cut(s) 150
AvaII GGWCC 1 cut(s) 648
AxyI CCTNAGG 1 cut(s) 72
BccI CCATC 1 cut(s) 75
BciT130I CCWGG 2 cut(s) 420, 658
BclI TGATCA 1 cut(s) 285
BcnI CCSGG 1 cut(s) 653
BcoDI GTCTC 1 cut(s) 176
BcuI ACTAGT 1 cut(s) 214
BfaI CTAG 2 cut(s) 90, 215
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 209
BisI GCNGC 2 cut(s) 684, 694
BlsI GCNGC 2 cut(s) 685, 695
Bme1390I CCNGG 3 cut(s) 420, 653, 658
Bme18I GGWCC 1 cut(s) 648
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 4 cut(s) 45, 297, 433, 648
BmiI GGNNCC 1 cut(s) 650
BmrFI CCNGG 3 cut(s) 420, 653, 658
BmsI GCATC 1 cut(s) 94
BpmI CTGGAG 2 cut(s) 578, 679
Bpu14I TTCGAA 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 653
BsaJI CCNNGG 2 cut(s) 418, 651
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 424, 425, 442, 657
Bse1I ACTGG 3 cut(s) 42, 105, 561
Bse21I CCTNAGG 1 cut(s) 72
BseBI CCWGG 2 cut(s) 420, 658
BseDI CCNNGG 2 cut(s) 418, 651
BseGI GGATG 4 cut(s) 26, 67, 109, 331
BseLI CCNNNNNNNGG 5 cut(s) 73, 424, 425, 442, 657
BseMII CTCAG 3 cut(s) 63, 171, 339
BseNI ACTGG 3 cut(s) 42, 105, 561
BseRI GAGGAG 1 cut(s) 613
BsgI GTGCAG 1 cut(s) 226
Bsh1236I CGCG 3 cut(s) 293, 683, 688
BshFI GGCC 4 cut(s) 47, 299, 434, 656
BsiSI CCGG 1 cut(s) 653
BslFI GGGAC 2 cut(s) 634, 647
BslI CCNNNNNNNGG 5 cut(s) 73, 424, 425, 442, 657
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 2 cut(s) 634, 647
BsnI GGCC 4 cut(s) 47, 299, 434, 656
Bsp119I TTCGAA 1 cut(s) 150
Bsp143I GATC 4 cut(s) 285, 310, 328, 461
BspACI CCGC 5 cut(s) 11, 190, 577, 683, 693
BspANI GGCC 4 cut(s) 47, 299, 434, 656
BspCNI CTCAG 3 cut(s) 64, 172, 340
BspFNI CGCG 3 cut(s) 293, 683, 688
BspLI GGNNCC 1 cut(s) 650
BspPI GGATC 3 cut(s) 305, 323, 469
BspQI GCTCTTC 2 cut(s) 366, 403
BspT104I TTCGAA 1 cut(s) 150
BsrI ACTGG 3 cut(s) 42, 105, 561
BssECI CCNNGG 2 cut(s) 418, 651
BssMI GATC 4 cut(s) 285, 310, 328, 461
Bst2UI CCWGG 2 cut(s) 420, 658
Bst6I CTCTTC 2 cut(s) 366, 403
BstBI TTCGAA 1 cut(s) 150
BstC8I GCNNGC 3 cut(s) 415, 577, 605
BstDEI CTNAG 3 cut(s) 72, 180, 348
BstF5I GGATG 4 cut(s) 26, 67, 109, 331
BstFNI CGCG 3 cut(s) 293, 683, 688
BstH2I RGCGCY 1 cut(s) 209
BstHHI GCGC 3 cut(s) 208, 683, 688
BstKTI GATC 4 cut(s) 288, 313, 331, 464
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 4 cut(s) 285, 310, 328, 461
BstMWI GCNNNNNNNGC 3 cut(s) 382, 576, 609
BstNI CCWGG 2 cut(s) 420, 658
BstSCI CCNGG 3 cut(s) 418, 651, 656
BstSFI CTRYAG 1 cut(s) 201
BstUI CGCG 3 cut(s) 293, 683, 688
BstX2I RGATCY 1 cut(s) 310
BstXI CCANNNNNNTGG 1 cut(s) 321
BstYI RGATCY 1 cut(s) 310
Bsu36I CCTNAGG 1 cut(s) 72
BsuRI GGCC 4 cut(s) 47, 299, 434, 656
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 4 cut(s) 26, 67, 109, 331
BtsIMutI CAGTG 3 cut(s) 35, 502, 554
Cac8I GCNNGC 3 cut(s) 415, 577, 605
CfoI GCGC 3 cut(s) 208, 683, 688
Cfr13I GGNCC 4 cut(s) 45, 297, 433, 648
Csp6I GTAC 3 cut(s) 15, 196, 218
CviAII CATG 3 cut(s) 49, 267, 388
CviQI GTAC 3 cut(s) 15, 196, 218
DdeI CTNAG 3 cut(s) 72, 180, 348
DpnI GATC 4 cut(s) 287, 312, 330, 463
DpnII GATC 4 cut(s) 285, 310, 328, 461
Eam1104I CTCTTC 2 cut(s) 366, 403
EarI CTCTTC 2 cut(s) 366, 403
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 648
Eco81I CCTNAGG 1 cut(s) 72
EcoRII CCWGG 2 cut(s) 418, 656
EcoRV GATATC 1 cut(s) 145
FaeI CATG 3 cut(s) 52, 270, 391
FalI AAGNNNNNCTT 2 cut(s) 397, 429
FaqI GGGAC 2 cut(s) 634, 647
FatI CATG 3 cut(s) 48, 266, 387
FauI CCCGC 2 cut(s) 4, 584
FbaI TGATCA 1 cut(s) 285
Fnu4HI GCNGC 2 cut(s) 684, 694
FokI GGATG 4 cut(s) 13, 54, 116, 338
Fsp4HI GCNGC 2 cut(s) 684, 694
FspBI CTAG 2 cut(s) 90, 215
GlaI GCGC 3 cut(s) 207, 682, 687
GluI GCNGC 2 cut(s) 684, 694
GsuI CTGGAG 2 cut(s) 578, 679
HaeII RGCGCY 1 cut(s) 209
HaeIII GGCC 4 cut(s) 47, 299, 434, 656
HapII CCGG 1 cut(s) 653
HhaI GCGC 3 cut(s) 208, 683, 688
Hin1II CATG 3 cut(s) 52, 270, 391
Hin6I GCGC 3 cut(s) 206, 681, 686
HinP1I GCGC 3 cut(s) 206, 681, 686
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 4 cut(s) 20, 184, 255, 531
HpaII CCGG 1 cut(s) 653
HphI GGTGA 1 cut(s) 607
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 2 cut(s) 181, 235
Hpy188III TCNNGA 2 cut(s) 71, 332
Hpy8I GTNNAC 1 cut(s) 589
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 499, 515
HpyCH4IV ACGT 2 cut(s) 261, 439
HpyCH4V TGCA 3 cut(s) 243, 359, 570
HpyF10VI GCNNNNNNNGC 3 cut(s) 382, 576, 609
HpyF3I CTNAG 3 cut(s) 72, 180, 348
HpySE526I ACGT 2 cut(s) 261, 439
Hsp92II CATG 3 cut(s) 52, 270, 391
HspAI GCGC 3 cut(s) 206, 681, 686
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 4 cut(s) 285, 310, 328, 461
LguI GCTCTTC 2 cut(s) 366, 403
LmnI GCTCC 2 cut(s) 600, 660
LweI GCATC 1 cut(s) 94
MaeI CTAG 2 cut(s) 90, 215
MaeII ACGT 2 cut(s) 261, 439
MaeIII GTNAC 1 cut(s) 269
MalI GATC 4 cut(s) 287, 312, 330, 463
MboI GATC 4 cut(s) 285, 310, 328, 461
MboII GAAGA 4 cut(s) 139, 383, 417, 420
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 1 cut(s) 310
MluCI AATT 3 cut(s) 110, 152, 582
MluI ACGCGT 1 cut(s) 291
MlyI GAGTC 2 cut(s) 178, 525
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 5 cut(s) 67, 245, 345, 553, 591
MseI TTAA 2 cut(s) 128, 452
MslI CAYNNNNRTG 1 cut(s) 40
MspI CCGG 1 cut(s) 653
MspR9I CCNGG 3 cut(s) 420, 653, 658
MteI GCGCNGCGC 1 cut(s) 684
MunI CAATTG 1 cut(s) 582
MvaI CCWGG 2 cut(s) 420, 658
MvnI CGCG 3 cut(s) 293, 683, 688
MwoI GCNNNNNNNGC 3 cut(s) 382, 576, 609
NciI CCSGG 1 cut(s) 653
NdeII GATC 4 cut(s) 285, 310, 328, 461
NlaIII CATG 3 cut(s) 52, 270, 391
NlaIV GGNNCC 1 cut(s) 650
NmeAIII GCCGAG 1 cut(s) 633
NmuCI GTSAC 1 cut(s) 269
NspV TTCGAA 1 cut(s) 150
PciSI GCTCTTC 2 cut(s) 366, 403
PfeI GAWTC 2 cut(s) 20, 255
PflMI CCANNNNNTGG 1 cut(s) 442
PkrI GCNGC 2 cut(s) 685, 695
PleI GAGTC 2 cut(s) 178, 525
PpsI GAGTC 2 cut(s) 178, 525
Psp6I CCWGG 2 cut(s) 418, 656
PspGI CCWGG 2 cut(s) 418, 656
PspN4I GGNNCC 1 cut(s) 650
PspPI GGNCC 4 cut(s) 45, 297, 433, 648
PsuI RGATCY 1 cut(s) 310
RsaI GTAC 3 cut(s) 16, 197, 219
RsaNI GTAC 3 cut(s) 15, 196, 218
RseI CAYNNNNRTG 1 cut(s) 40
SapI GCTCTTC 2 cut(s) 366, 403
SaqAI TTAA 2 cut(s) 128, 452
SatI GCNGC 2 cut(s) 684, 694
Sau3AI GATC 4 cut(s) 285, 310, 328, 461
Sau96I GGNCC 4 cut(s) 45, 297, 433, 648
SchI GAGTC 2 cut(s) 178, 525
ScrFI CCNGG 3 cut(s) 420, 653, 658
SfaNI GCATC 1 cut(s) 94
SfcI CTRYAG 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 150
SinI GGWCC 1 cut(s) 648
SmiMI CAYNNNNRTG 1 cut(s) 40
SpeI ACTAGT 1 cut(s) 214
Sse9I AATT 3 cut(s) 110, 152, 582
SsiI CCGC 5 cut(s) 11, 190, 577, 683, 693
SspMI CTAG 2 cut(s) 90, 215
StyD4I CCNGG 3 cut(s) 418, 651, 656
TaiI ACGT 2 cut(s) 264, 442
TaqI TCGA 5 cut(s) 150, 258, 480, 626, 666
TaqII GACCGA 1 cut(s) 636
TasI AATT 3 cut(s) 110, 152, 582
TauI GCSGC 2 cut(s) 686, 696
TfiI GAWTC 2 cut(s) 20, 255
Tru1I TTAA 2 cut(s) 128, 452
Tru9I TTAA 2 cut(s) 128, 452
TscAI CASTG 3 cut(s) 42, 502, 561
TseFI GTSAC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 2 cut(s) 150, 518
TspRI CASTG 3 cut(s) 42, 502, 561
Van91I CCANNNNNTGG 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 648
XapI RAATTY 1 cut(s) 110
XcmI CCANNNNNNNNNTGG 1 cut(s) 443
XspI CTAG 2 cut(s) 90, 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.