Rroxscaffold_2G00136750

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000218
Physical Location & Seq
Forward (+)
74436962 .. 74437603
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136750.1

Sequence Viewer

Length: 642 bp
ATGCTTGCAGGCAAACACATGGGCTACAACTACACCACTGTGGTGGAGTCCCCATATGGGGATCACTGGCGCAACCTACGCCGTATTGGCGCTACCGATATCTACTCTCCCACTAGGCTCAACATGTTCTCGAGCGTTAGGAAGGACGAAGTCAAGCAATTGCTGTTCAGACTCTCACACAACTCACGTGAAGATTTTGTAAAGGTGGACTTGAGATCCATGCTCTCTGAGCTGACATTTAACATCATAATGACAATGGTGGCAGGGAAGAGGTATTTTGGAGATGGCATTTCAGACAAAGAAGAGGCCAAACAGTTCAGAGATCTCATGAGCGAGGGTTTGTCGTATGGTGGCGCAACAAACCCCTCAGACTTTGTGCCAATATTGAAATGGTTTGGAGGTCGTGGGGTTGAGAAGAAGGTGATGGACTTGGGTAAGAGGATGGATGCATTCTTGCAAGGTTTGATCGATGAGCAGAATTTGAGTAAAACTACTTCAGATTGGAGTACAGCAAATACTTTGATCCACCATTTACTTTCTCTTCAAGAATCACAGCCTGAGTATTACACTGACCAAATTATCAAAGGGCTTATACTGGTTAGTACAATCATTCAAACTTTGCATCAAGCTACATGGATATAA

Protein Analysis

213

Amino Acids

24.36

Weight (kDa)

7.03

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 13 - 197 2.4e-09 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66540 AT1G66540 AT4G37340 AT4G37360 AT4G37370 AT5G36220 AT5G36220
fragaria_vesca FvH4_1g16862 FvH4_1g16870 FvH4_1g16871 FvH4_1g16920 FvH4_1g16921 FvH4_1g16930 FvH4_1g23681 FvH4_1g23710
malus_domestica MD03G1281500.v1.1 MD08G1089700.v1.1 MD15G1074700.v1.1 MD15G1289800.v1.1 MD15G1290300.v1.1
prunus_persica Prupe.6G226600_v2.0.a1 Prupe.6G226800_v2.0.a1 Prupe.6G226900_v2.0.a1 Prupe.6G227000_v2.0.a1 Prupe.6G227100_v2.0.a1 Prupe.6G227300_v2.0.a1 Prupe.6G227400_v2.0.a1
pyrus_communis pycom15g25350 pycom15g25360 pycom15g25370 pycom15g25400
rosa_chinensis RchiOBHm_Chr2g0106831 RchiOBHm_Chr2g0106851 RchiOBHm_Chr2g0106901 RchiOBHm_Chr2g0106911 RchiOBHm_Chr2g0106971 RchiOBHm_Chr2g0106991 RchiOBHm_Chr2g0119331 RchiOBHm_Chr2g0119351 RchiOBHm_Chr2g0119391 RchiOBHm_Chr2g0119411 RchiOBHm_Chr2g0119481 RchiOBHm_Chr2g0119541 RchiOBHm_Chr5g0076421
rosa_laevigata RLG00000012204 RLG00000017542 RLG00000017543 RLG00000017547 RLG00000017549 RLG00000018145 RLG00000018146 RLG00000018148 RLG00000018462 RLG00000018464 RLG00000018465 RLG00000018468 RLG00000018470 RLG00000018471 RLG00000018539 RLG00000018540 RLG00000018592
rosa_multiflora Rmu_co8030410.1_g000001 Rmu_co8333451.1_g000001 Rmu_co8389363.1_g000001 Rmu_sc0001801.1_g000006 Rmu_sc0001801.1_g000023 Rmu_sc0001801.1_g000036 Rmu_sc0002788.1_g000013 Rmu_sc0002868.1_g000014 Rmu_sc0004344.1_g000013 Rmu_sc0004344.1_g000021 Rmu_sc0004344.1_g000027 Rmu_sc0004656.1_g000001 Rmu_sc0006098.1_g000002 Rmu_sc0006098.1_g000007 Rmu_sc0007106.1_g000005 Rmu_sc0007106.1_g000009 Rmu_sc0010912.1_g000001 Rmu_sc0021576.1_g000001 Rmu_sc0031470.1_g000001
rosa_roxburghii Rroxscaffold_2G00122810 Rroxscaffold_2G00123600 Rroxscaffold_2G00124270 Rroxscaffold_2G00124320 Rroxscaffold_2G00124350 Rroxscaffold_2G00136690 Rroxscaffold_2G00136740 Rroxscaffold_2G00136750 Rroxscaffold_2G00136760
rosa_rugosa Rorug02G0139000 Rorug02G0139100 Rorug02G0139200 Rorug02G0139300 Rorug02G0139700 Rorug02G0139900 Rorug02G0221400 Rorug02G0221900 Rorug02G0222100 Rorug02G0231700 Rorug02G0231800 Rorug02G0238400 Rorug02G0238500 Rorug05G0413800 Rorug06G0369500 Rorug07G0161900 Rorug07G0309500.1
rosa_samantha Rh2AG190700 Rh2AG279600 Rh2BG201600 Rh2BG201900 Rh2BG202200 Rh2BG288600 Rh2BG290400 Rh2BG290600 Rh2BG291000 Rh2BG291100 Rh2BG291200 Rh2BG291300 Rh2CG195400 Rh2DG196900 Rh2DG197400 Rh2DG197700 Rh2DG283900 Rh2DG285600 Rh2DG285700 Rh2DG286000 Rh2DG286100 Rh2DG286200 Rh2DG286300 Rh2DG305000 Rh2DG305100 Rh2DG305200 Rh2DG305400 Rh5DG500100 Rh6AG481700 Rh6BG491200 Rh6DG482200
rosa_wichuraiana Rw2G014970 Rw2G015010 Rw2G015030 Rw2G022240 Rw2G022250 Rw2G022290 Rw2G022300 Rw2G022310 Rw2G022320 Rw5G034260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 69, 210, 517
AcsI RAATTY 1 cut(s) 478
AcuI CTGAAG 1 cut(s) 480
AcvI CACGTG 1 cut(s) 188
AfaI GTAC 2 cut(s) 508, 604
AfiI CCNNNNNNNGG 2 cut(s) 57, 58
AflIII ACRYGT 1 cut(s) 123
AgsI TTSAA 3 cut(s) 388, 545, 614
AleI CACNNNNGTG 2 cut(s) 38, 41
AluBI AGCT 2 cut(s) 232, 629
AluI AGCT 2 cut(s) 232, 629
AlwI GGATC 3 cut(s) 69, 210, 517
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 1 cut(s) 306
ApoI RAATTY 1 cut(s) 478
AspLEI GCGC 3 cut(s) 72, 92, 356
AsuHPI GGTGA 1 cut(s) 433
AvaI CYCGRG 1 cut(s) 130
BarI GAAGNNNNNNTAC 2 cut(s) 478, 510
BbrPI CACGTG 1 cut(s) 188
BccI CCATC 3 cut(s) 278, 418, 436
BceAI ACGGC 1 cut(s) 66
BfaI CTAG 1 cut(s) 114
BfoI RGCGCY 1 cut(s) 93
BglI GCCNNNNNGGC 1 cut(s) 87
BglII AGATCT 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 130
BmsI GCATC 2 cut(s) 436, 631
BpuEI CTTGAG 1 cut(s) 232
Bsa29I ATCGAT 1 cut(s) 468
BsaAI YACGTR 1 cut(s) 188
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 58
Bse1I ACTGG 2 cut(s) 71, 600
BseCI ATCGAT 1 cut(s) 468
BseGI GGATG 2 cut(s) 447, 451
BseLI CCNNNNNNNGG 2 cut(s) 57, 58
BseMII CTCAG 3 cut(s) 219, 381, 549
BseNI ACTGG 2 cut(s) 71, 600
BshFI GGCC 1 cut(s) 308
BshVI ATCGAT 1 cut(s) 468
BsiHKCI CYCGRG 1 cut(s) 130
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 2 cut(s) 57, 58
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 449
BsnI GGCC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 130
Bsp143I GATC 5 cut(s) 61, 215, 322, 465, 522
BspANI GGCC 1 cut(s) 308
BspCNI CTCAG 3 cut(s) 220, 380, 550
BspDI ATCGAT 1 cut(s) 468
BspHI TCATGA 1 cut(s) 327
BspPI GGATC 3 cut(s) 69, 210, 517
BsrI ACTGG 2 cut(s) 71, 600
BssMI GATC 5 cut(s) 61, 215, 322, 465, 522
Bst4CI ACNGT 2 cut(s) 40, 315
Bst6I CTCTTC 3 cut(s) 263, 297, 546
BstBAI YACGTR 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 6, 10
BstDEI CTNAG 3 cut(s) 228, 367, 558
BstF5I GGATG 2 cut(s) 447, 451
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 3 cut(s) 72, 92, 356
BstKTI GATC 5 cut(s) 64, 218, 325, 468, 525
BstMBI GATC 5 cut(s) 61, 215, 322, 465, 522
BstMWI GCNNNNNNNGC 3 cut(s) 78, 87, 229
BstNSI RCATGY 1 cut(s) 127
BstX2I RGATCY 2 cut(s) 215, 322
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 2 cut(s) 215, 322
Bsu15I ATCGAT 1 cut(s) 468
BsuRI GGCC 1 cut(s) 308
BsuTUI ATCGAT 1 cut(s) 468
BtsCI GGATG 2 cut(s) 447, 451
BtsIMutI CAGTG 3 cut(s) 36, 64, 567
Cac8I GCNNGC 2 cut(s) 6, 10
CciI TCATGA 1 cut(s) 327
CfoI GCGC 3 cut(s) 72, 92, 356
ClaI ATCGAT 1 cut(s) 468
Csp6I GTAC 2 cut(s) 507, 603
CviAII CATG 5 cut(s) 19, 124, 220, 328, 633
CviJI RGCY 7 cut(s) 24, 118, 232, 308, 556, 589, 629
CviKI_1 RGCY 7 cut(s) 24, 118, 232, 308, 556, 589, 629
CviQI GTAC 2 cut(s) 507, 603
DdeI CTNAG 3 cut(s) 228, 367, 558
DpnI GATC 5 cut(s) 63, 217, 324, 467, 524
DpnII GATC 5 cut(s) 61, 215, 322, 465, 522
Eam1104I CTCTTC 3 cut(s) 263, 297, 546
EarI CTCTTC 3 cut(s) 263, 297, 546
Eco32I GATATC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 480
Eco72I CACGTG 1 cut(s) 188
Eco88I CYCGRG 1 cut(s) 130
EcoRV GATATC 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 451
FaeI CATG 5 cut(s) 22, 127, 223, 331, 636
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 1 cut(s) 34
FatI CATG 5 cut(s) 18, 123, 219, 327, 632
FauNDI CATATG 1 cut(s) 55
FokI GGATG 2 cut(s) 454, 458
FspBI CTAG 1 cut(s) 114
GlaI GCGC 3 cut(s) 71, 91, 355
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 1 cut(s) 308
HhaI GCGC 3 cut(s) 72, 92, 356
Hin1II CATG 5 cut(s) 22, 127, 223, 331, 636
Hin6I GCGC 3 cut(s) 70, 90, 354
HinP1I GCGC 3 cut(s) 70, 90, 354
HinfI GANTC 3 cut(s) 47, 171, 548
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 6 cut(s) 170, 229, 295, 320, 370, 499
Hpy188III TCNNGA 3 cut(s) 130, 328, 545
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 2 cut(s) 136, 412
HpyCH4III ACNGT 2 cut(s) 40, 315
HpyCH4IV ACGT 1 cut(s) 187
HpyCH4V TGCA 4 cut(s) 8, 449, 457, 622
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 87, 229
HpyF3I CTNAG 3 cut(s) 228, 367, 558
HpySE526I ACGT 1 cut(s) 187
Hsp92II CATG 5 cut(s) 22, 127, 223, 331, 636
HspAI GCGC 3 cut(s) 70, 90, 354
Kzo9I GATC 5 cut(s) 61, 215, 322, 465, 522
LpnPI CCDG 4 cut(s) 52, 249, 570, 581
LweI GCATC 2 cut(s) 436, 631
MaeI CTAG 1 cut(s) 114
MaeII ACGT 1 cut(s) 187
MalI GATC 5 cut(s) 63, 217, 324, 467, 524
MboI GATC 5 cut(s) 61, 215, 322, 465, 522
MboII GAAGA 5 cut(s) 203, 280, 314, 427, 533
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 2 cut(s) 215, 322
MluCI AATT 3 cut(s) 158, 478, 576
MlyI GAGTC 2 cut(s) 56, 165
MnlI CCTC 6 cut(s) 264, 298, 328, 376, 392, 432
Mph1103I ATGCAT 1 cut(s) 451
MseI TTAA 1 cut(s) 240
MslI CAYNNNNRTG 3 cut(s) 38, 41, 248
MunI CAATTG 1 cut(s) 158
Mva1269I GAATGC 1 cut(s) 449
MwoI GCNNNNNNNGC 3 cut(s) 78, 87, 229
NdeI CATATG 1 cut(s) 55
NdeII GATC 5 cut(s) 61, 215, 322, 465, 522
NlaIII CATG 5 cut(s) 22, 127, 223, 331, 636
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 1 cut(s) 127
OliI CACNNNNGTG 2 cut(s) 38, 41
PaeR7I CTCGAG 1 cut(s) 130
PagI TCATGA 1 cut(s) 327
PciI ACATGT 1 cut(s) 123
PctI GAATGC 1 cut(s) 449
PfeI GAWTC 1 cut(s) 548
PflFI GACNNNGTC 1 cut(s) 149
PleI GAGTC 2 cut(s) 55, 165
PmaCI CACGTG 1 cut(s) 188
PmlI CACGTG 1 cut(s) 188
PpsI GAGTC 2 cut(s) 55, 165
Ppu21I YACGTR 1 cut(s) 188
PscI ACATGT 1 cut(s) 123
PspCI CACGTG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 215, 322
PsyI GACNNNGTC 1 cut(s) 149
RsaI GTAC 2 cut(s) 508, 604
RsaNI GTAC 2 cut(s) 507, 603
RseI CAYNNNNRTG 3 cut(s) 38, 41, 248
SaqAI TTAA 1 cut(s) 240
Sau3AI GATC 5 cut(s) 61, 215, 322, 465, 522
SchI GAGTC 2 cut(s) 56, 165
SetI ASST 9 cut(s) 78, 190, 207, 234, 275, 403, 423, 463, 631
SfaNI GCATC 2 cut(s) 436, 631
Sfr274I CTCGAG 1 cut(s) 130
SlaI CTCGAG 1 cut(s) 130
SmiMI CAYNNNNRTG 3 cut(s) 38, 41, 248
SmlI CTYRAG 2 cut(s) 130, 211
SmoI CTYRAG 2 cut(s) 130, 211
Sse9I AATT 3 cut(s) 158, 478, 576
SspI AATATT 1 cut(s) 384
SspMI CTAG 1 cut(s) 114
TaaI ACNGT 2 cut(s) 40, 315
TaiI ACGT 1 cut(s) 190
TaqI TCGA 2 cut(s) 131, 468
TasI AATT 3 cut(s) 158, 478, 576
TatI WGTACW 2 cut(s) 506, 602
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 3 cut(s) 43, 71, 574
TspRI CASTG 3 cut(s) 43, 71, 574
Tth111I GACNNNGTC 1 cut(s) 149
XapI RAATTY 1 cut(s) 478
XceI RCATGY 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 387
XhoI CTCGAG 1 cut(s) 130
XspI CTAG 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.