Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 716 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 131, 1357 |
| AccII | CGCG | 2 cut(s) | 231, 1208 |
| AciI | CCGC | 6 cut(s) | 88, 394, 405, 926, 1105, 1492 |
| AclWI | GGATC | 5 cut(s) | 522, 832, 1220, 1238, 1384 |
| AcoI | YGGCCR | 1 cut(s) | 133 |
| AcsI | RAATTY | 1 cut(s) | 1025 |
| AcvI | CACGTG | 1 cut(s) | 500 |
| AcyI | GRCGYC | 1 cut(s) | 717 |
| AfaI | GTAC | 4 cut(s) | 832, 931, 1112, 1134 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 131, 988, 1339, 1340, 1357 |
| AflIII | ACRYGT | 2 cut(s) | 1206, 1354 |
| AgsI | TTSAA | 6 cut(s) | 61, 481, 553, 700, 1258, 1388 |
| AhlI | ACTAGT | 1 cut(s) | 1129 |
| AjiI | CACGTC | 2 cut(s) | 227, 1177 |
| AjnI | CCWGG | 1 cut(s) | 1333 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 100, 132 |
| AleI | CACNNNNGTG | 1 cut(s) | 353 |
| AluBI | AGCT | 6 cut(s) | 495, 796, 1010, 1254, 1300, 1463 |
| AluI | AGCT | 6 cut(s) | 495, 796, 1010, 1254, 1300, 1463 |
| Alw26I | GTCTC | 2 cut(s) | 366, 1091 |
| AlwI | GGATC | 5 cut(s) | 522, 832, 1220, 1238, 1384 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 796 |
| AoxI | GGCC | 5 cut(s) | 133, 196, 960, 1212, 1347 |
| ApeKI | GCWGC | 3 cut(s) | 492, 790, 793 |
| ApoI | RAATTY | 1 cut(s) | 1025 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 764 |
| AspA2I | CCTAGG | 1 cut(s) | 331 |
| AspLEI | GCGC | 3 cut(s) | 384, 719, 1123 |
| AspS9I | GGNCC | 4 cut(s) | 197, 960, 1212, 1348 |
| AsuC2I | CCSGG | 1 cut(s) | 721 |
| AsuHPI | GGTGA | 2 cut(s) | 265, 748 |
| AsuII | TTCGAA | 2 cut(s) | 73, 1065 |
| AvrII | CCTAGG | 1 cut(s) | 331 |
| AxyI | CCTNAGG | 1 cut(s) | 987 |
| BalI | TGGCCA | 1 cut(s) | 135 |
| BanI | GGYRCC | 1 cut(s) | 716 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 922, 954 |
| BbrPI | CACGTG | 1 cut(s) | 500 |
| BbsI | GAAGAC | 2 cut(s) | 237, 1054 |
| BbvI | GCAGC | 3 cut(s) | 504, 802, 805 |
| BccI | CCATC | 4 cut(s) | 144, 150, 733, 990 |
| BceAI | ACGGC | 1 cut(s) | 273 |
| BciT130I | CCWGG | 1 cut(s) | 1335 |
| BclI | TGATCA | 1 cut(s) | 1200 |
| BcnI | CCSGG | 1 cut(s) | 721 |
| BcoDI | GTCTC | 2 cut(s) | 366, 1091 |
| BcuI | ACTAGT | 1 cut(s) | 1129 |
| BfaI | CTAG | 3 cut(s) | 332, 1005, 1130 |
| BfmI | CTRYAG | 1 cut(s) | 1116 |
| BfoI | RGCGCY | 2 cut(s) | 720, 1124 |
| BglII | AGATCT | 1 cut(s) | 411 |
| BisI | GCNGC | 4 cut(s) | 394, 493, 791, 794 |
| BlnI | CCTAGG | 1 cut(s) | 331 |
| BlsI | GCNGC | 4 cut(s) | 395, 494, 792, 795 |
| Bme1390I | CCNGG | 2 cut(s) | 721, 1335 |
| BmgBI | CACGTC | 2 cut(s) | 227, 1177 |
| BmgT120I | GGNCC | 4 cut(s) | 197, 960, 1212, 1348 |
| BmiI | GGNNCC | 3 cut(s) | 199, 403, 718 |
| BmrFI | CCNGG | 2 cut(s) | 721, 1335 |
| BmsI | GCATC | 2 cut(s) | 456, 1009 |
| BpiI | GAAGAC | 2 cut(s) | 237, 1054 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 401, 433 |
| BpmI | CTGGAG | 1 cut(s) | 1493 |
| Bpu10I | CCTNAGC | 1 cut(s) | 149 |
| Bpu14I | TTCGAA | 2 cut(s) | 73, 1065 |
| BpuEI | CTTGAG | 3 cut(s) | 246, 544, 766 |
| BpuMI | CCSGG | 1 cut(s) | 721 |
| Bsa29I | ATCGAT | 1 cut(s) | 783 |
| BsaAI | YACGTR | 1 cut(s) | 500 |
| BsaHI | GRCGYC | 1 cut(s) | 717 |
| BsaJI | CCNNGG | 2 cut(s) | 331, 1333 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1466, 1496 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 131, 988, 1339, 1340, 1357 |
| Bse1I | ACTGG | 5 cut(s) | 125, 383, 423, 1020, 1476 |
| Bse21I | CCTNAGG | 1 cut(s) | 987 |
| BseBI | CCWGG | 1 cut(s) | 1335 |
| BseCI | ATCGAT | 1 cut(s) | 783 |
| BseDI | CCNNGG | 2 cut(s) | 331, 1333 |
| BseGI | GGATG | 9 cut(s) | 10, 42, 248, 463, 762, 941, 982, 1024, 1246 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 131, 988, 1339, 1340, 1357 |
| BseMII | CTCAG | 5 cut(s) | 531, 693, 788, 978, 1254 |
| BseNI | ACTGG | 5 cut(s) | 125, 383, 423, 1020, 1476 |
| BseXI | GCAGC | 3 cut(s) | 504, 802, 805 |
| Bsh1236I | CGCG | 2 cut(s) | 231, 1208 |
| BshFI | GGCC | 5 cut(s) | 135, 198, 962, 1214, 1349 |
| BshNI | GGYRCC | 1 cut(s) | 716 |
| BshVI | ATCGAT | 1 cut(s) | 783 |
| BsiSI | CCGG | 2 cut(s) | 720, 872 |
| BslFI | GGGAC | 2 cut(s) | 16, 608 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 131, 988, 1339, 1340, 1357 |
| BsmAI | GTCTC | 2 cut(s) | 366, 1091 |
| BsmFI | GGGAC | 2 cut(s) | 16, 608 |
| BsmI | GAATGC | 1 cut(s) | 766 |
| BsnI | GGCC | 5 cut(s) | 135, 198, 962, 1214, 1349 |
| Bsp119I | TTCGAA | 2 cut(s) | 73, 1065 |
| BspACI | CCGC | 6 cut(s) | 88, 394, 405, 926, 1105, 1492 |
| BspANI | GGCC | 5 cut(s) | 135, 198, 962, 1214, 1349 |
| BspCNI | CTCAG | 5 cut(s) | 532, 692, 789, 979, 1255 |
| BspDI | ATCGAT | 1 cut(s) | 783 |
| BspFNI | CGCG | 2 cut(s) | 231, 1208 |
| BspHI | TCATGA | 1 cut(s) | 639 |
| BspLI | GGNNCC | 3 cut(s) | 199, 403, 718 |
| BspPI | GGATC | 5 cut(s) | 522, 832, 1220, 1238, 1384 |
| BspQI | GCTCTTC | 1 cut(s) | 1281 |
| BspT104I | TTCGAA | 2 cut(s) | 73, 1065 |
| BspT107I | GGYRCC | 1 cut(s) | 716 |
| BsrI | ACTGG | 5 cut(s) | 125, 383, 423, 1020, 1476 |
| BssECI | CCNNGG | 2 cut(s) | 331, 1333 |
| BssNI | GRCGYC | 1 cut(s) | 717 |
| BssT1I | CCWWGG | 1 cut(s) | 331 |
| Bst2UI | CCWGG | 1 cut(s) | 1335 |
| Bst4CI | ACNGT | 3 cut(s) | 302, 352, 627 |
| Bst6I | CTCTTC | 3 cut(s) | 126, 575, 1281 |
| BstACI | GRCGYC | 1 cut(s) | 717 |
| BstBAI | YACGTR | 1 cut(s) | 500 |
| BstBI | TTCGAA | 2 cut(s) | 73, 1065 |
| BstC8I | GCNNGC | 3 cut(s) | 326, 1330, 1492 |
| BstDEI | CTNAG | 7 cut(s) | 149, 540, 679, 797, 987, 1263, 1518 |
| BstF5I | GGATG | 9 cut(s) | 10, 42, 248, 463, 762, 941, 982, 1024, 1246 |
| BstFNI | CGCG | 2 cut(s) | 231, 1208 |
| BstH2I | RGCGCY | 2 cut(s) | 720, 1124 |
| BstHHI | GCGC | 3 cut(s) | 384, 719, 1123 |
| BstMAI | GTCTC | 2 cut(s) | 366, 1091 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 228, 390, 1297, 1491 |
| BstNI | CCWGG | 1 cut(s) | 1335 |
| BstSCI | CCNGG | 2 cut(s) | 719, 1333 |
| BstSFI | CTRYAG | 1 cut(s) | 1116 |
| BstUI | CGCG | 2 cut(s) | 231, 1208 |
| BstV1I | GCAGC | 3 cut(s) | 504, 802, 805 |
| BstV2I | GAAGAC | 2 cut(s) | 237, 1054 |
| BstX2I | RGATCY | 3 cut(s) | 411, 527, 1225 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 355, 1236 |
| BstYI | RGATCY | 3 cut(s) | 411, 527, 1225 |
| Bsu15I | ATCGAT | 1 cut(s) | 783 |
| Bsu36I | CCTNAGG | 1 cut(s) | 987 |
| BsuRI | GGCC | 5 cut(s) | 135, 198, 962, 1214, 1349 |
| BsuTUI | ATCGAT | 1 cut(s) | 783 |
| BtrI | CACGTC | 2 cut(s) | 227, 1177 |
| BtsCI | GGATG | 9 cut(s) | 10, 42, 248, 463, 762, 941, 982, 1024, 1246 |
| BtsIMutI | CAGTG | 3 cut(s) | 376, 1417, 1469 |
| Cac8I | GCNNGC | 3 cut(s) | 326, 1330, 1492 |
| CaiI | CAGNNNCTG | 1 cut(s) | 796 |
| CciI | TCATGA | 1 cut(s) | 639 |
| CfoI | GCGC | 3 cut(s) | 384, 719, 1123 |
| Cfr13I | GGNCC | 4 cut(s) | 197, 960, 1212, 1348 |
| ClaI | ATCGAT | 1 cut(s) | 783 |
| Csp6I | GTAC | 4 cut(s) | 831, 930, 1111, 1133 |
| CviAII | CATG | 7 cut(s) | 313, 532, 640, 916, 964, 1182, 1303 |
| CviQI | GTAC | 4 cut(s) | 831, 930, 1111, 1133 |
| DdeI | CTNAG | 7 cut(s) | 149, 540, 679, 797, 987, 1263, 1518 |
| DinI | GGCGCC | 1 cut(s) | 718 |
| EaeI | YGGCCR | 1 cut(s) | 133 |
| Eam1104I | CTCTTC | 3 cut(s) | 126, 575, 1281 |
| EarI | CTCTTC | 3 cut(s) | 126, 575, 1281 |
| Eco130I | CCWWGG | 1 cut(s) | 331 |
| Eco32I | GATATC | 1 cut(s) | 637 |
| Eco72I | CACGTG | 1 cut(s) | 500 |
| Eco81I | CCTNAGG | 1 cut(s) | 987 |
| EcoRII | CCWGG | 1 cut(s) | 1333 |
| EcoRV | GATATC | 1 cut(s) | 637 |
| EcoT14I | CCWWGG | 1 cut(s) | 331 |
| EgeI | GGCGCC | 1 cut(s) | 718 |
| EheI | GGCGCC | 1 cut(s) | 718 |
| ErhI | CCWWGG | 1 cut(s) | 331 |
| FaeI | CATG | 7 cut(s) | 316, 535, 643, 919, 967, 1185, 1306 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 100, 132, 506, 538, 1312, 1344 |
| FaqI | GGGAC | 2 cut(s) | 16, 608 |
| FatI | CATG | 7 cut(s) | 312, 531, 639, 915, 963, 1181, 1302 |
| FauI | CCCGC | 2 cut(s) | 919, 1499 |
| FauNDI | CATATG | 1 cut(s) | 658 |
| FbaI | TGATCA | 1 cut(s) | 1200 |
| Fnu4HI | GCNGC | 4 cut(s) | 394, 493, 791, 794 |
| FokI | GGATG | 9 cut(s) | 17, 29, 235, 470, 769, 928, 969, 1031, 1253 |
| Fsp4HI | GCNGC | 4 cut(s) | 394, 493, 791, 794 |
| FspBI | CTAG | 3 cut(s) | 332, 1005, 1130 |
| GlaI | GCGC | 3 cut(s) | 383, 718, 1122 |
| GluI | GCNGC | 4 cut(s) | 394, 493, 791, 794 |
| GsuI | CTGGAG | 1 cut(s) | 1493 |
| HaeII | RGCGCY | 2 cut(s) | 720, 1124 |
| HaeIII | GGCC | 5 cut(s) | 135, 198, 962, 1214, 1349 |
| HapII | CCGG | 2 cut(s) | 720, 872 |
| HhaI | GCGC | 3 cut(s) | 384, 719, 1123 |
| Hin1I | GRCGYC | 1 cut(s) | 717 |
| Hin1II | CATG | 7 cut(s) | 316, 535, 643, 919, 967, 1185, 1306 |
| Hin6I | GCGC | 3 cut(s) | 382, 717, 1121 |
| HinP1I | GCGC | 3 cut(s) | 382, 717, 1121 |
| HindIII | AAGCTT | 1 cut(s) | 1252 |
| HinfI | GANTC | 8 cut(s) | 359, 617, 863, 935, 1099, 1170, 1384, 1446 |
| HpaII | CCGG | 2 cut(s) | 720, 872 |
| HphI | GGTGA | 2 cut(s) | 265, 748 |
| Hpy166II | GTNNAC | 2 cut(s) | 520, 1504 |
| Hpy188I | TCNGA | 9 cut(s) | 452, 541, 607, 632, 682, 819, 868, 1096, 1150 |
| Hpy188III | TCNNGA | 5 cut(s) | 263, 466, 640, 986, 1247 |
| Hpy8I | GTNNAC | 2 cut(s) | 520, 1504 |
| Hpy99I | CGWCG | 2 cut(s) | 231, 1178 |
| HpyAV | CCTTC | 7 cut(s) | 126, 181, 559, 727, 1317, 1414, 1430 |
| HpyCH4III | ACNGT | 3 cut(s) | 302, 352, 627 |
| HpyCH4IV | ACGT | 4 cut(s) | 226, 499, 1176, 1354 |
| HpyCH4V | TGCA | 6 cut(s) | 512, 772, 859, 1158, 1274, 1485 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 228, 390, 1297, 1491 |
| HpyF3I | CTNAG | 7 cut(s) | 149, 540, 679, 797, 987, 1263, 1518 |
| HpySE526I | ACGT | 4 cut(s) | 226, 499, 1176, 1354 |
| Hsp92I | GRCGYC | 1 cut(s) | 717 |
| Hsp92II | CATG | 7 cut(s) | 316, 535, 643, 919, 967, 1185, 1306 |
| HspAI | GCGC | 3 cut(s) | 382, 717, 1121 |
| KasI | GGCGCC | 1 cut(s) | 716 |
| Ksp22I | TGATCA | 1 cut(s) | 1200 |
| LguI | GCTCTTC | 1 cut(s) | 1281 |
| Lsp1109I | GCAGC | 3 cut(s) | 504, 802, 805 |
| LweI | GCATC | 2 cut(s) | 456, 1009 |
| MaeI | CTAG | 3 cut(s) | 332, 1005, 1130 |
| MaeII | ACGT | 4 cut(s) | 226, 499, 1176, 1354 |
| MaeIII | GTNAC | 2 cut(s) | 667, 1184 |
| MfeI | CAATTG | 1 cut(s) | 1497 |
| MflI | RGATCY | 3 cut(s) | 411, 527, 1225 |
| MlsI | TGGCCA | 1 cut(s) | 135 |
| MluCI | AATT | 5 cut(s) | 470, 891, 1025, 1067, 1497 |
| MluI | ACGCGT | 1 cut(s) | 1206 |
| MluNI | TGGCCA | 1 cut(s) | 135 |
| Mly113I | GGCGCC | 1 cut(s) | 717 |
| MlyI | GAGTC | 4 cut(s) | 368, 626, 1093, 1440 |
| MmeI | TCCRAC | 1 cut(s) | 1253 |
| Mox20I | TGGCCA | 1 cut(s) | 135 |
| MroXI | GAANNNNTTC | 1 cut(s) | 764 |
| MscI | TGGCCA | 1 cut(s) | 135 |
| MseI | TTAA | 2 cut(s) | 1043, 1367 |
| MslI | CAYNNNNRTG | 3 cut(s) | 353, 560, 955 |
| Msp20I | TGGCCA | 1 cut(s) | 135 |
| MspA1I | CMGCKG | 1 cut(s) | 796 |
| MspI | CCGG | 2 cut(s) | 720, 872 |
| MspR9I | CCNGG | 2 cut(s) | 721, 1335 |
| MunI | CAATTG | 1 cut(s) | 1497 |
| Mva1269I | GAATGC | 1 cut(s) | 766 |
| MvaI | CCWGG | 1 cut(s) | 1335 |
| MvnI | CGCG | 2 cut(s) | 231, 1208 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 228, 390, 1297, 1491 |
| NarI | GGCGCC | 1 cut(s) | 717 |
| NciI | CCSGG | 1 cut(s) | 721 |
| NdeI | CATATG | 1 cut(s) | 658 |
| NlaIII | CATG | 7 cut(s) | 316, 535, 643, 919, 967, 1185, 1306 |
| NlaIV | GGNNCC | 3 cut(s) | 199, 403, 718 |
| NmuCI | GTSAC | 1 cut(s) | 1184 |
| NspV | TTCGAA | 2 cut(s) | 73, 1065 |
| OliI | CACNNNNGTG | 1 cut(s) | 353 |
| PagI | TCATGA | 1 cut(s) | 639 |
| PciSI | GCTCTTC | 1 cut(s) | 1281 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 496 |
| PctI | GAATGC | 1 cut(s) | 766 |
| PdmI | GAANNNNTTC | 1 cut(s) | 764 |
| PfeI | GAWTC | 4 cut(s) | 863, 935, 1170, 1384 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 131, 1357 |
| PkrI | GCNGC | 4 cut(s) | 395, 494, 792, 795 |
| PleI | GAGTC | 4 cut(s) | 367, 625, 1093, 1440 |
| PluTI | GGCGCC | 1 cut(s) | 720 |
| PmaCI | CACGTG | 1 cut(s) | 500 |
| PmlI | CACGTG | 1 cut(s) | 500 |
| PpsI | GAGTC | 4 cut(s) | 367, 625, 1093, 1440 |
| Ppu21I | YACGTR | 1 cut(s) | 500 |
| Psp6I | CCWGG | 1 cut(s) | 1333 |
| PspCI | CACGTG | 1 cut(s) | 500 |
| PspGI | CCWGG | 1 cut(s) | 1333 |
| PspN4I | GGNNCC | 3 cut(s) | 199, 403, 718 |
| PspPI | GGNCC | 4 cut(s) | 197, 960, 1212, 1348 |
| PstNI | CAGNNNCTG | 1 cut(s) | 796 |
| PsuI | RGATCY | 3 cut(s) | 411, 527, 1225 |
| PvuII | CAGCTG | 1 cut(s) | 796 |
| RsaI | GTAC | 4 cut(s) | 832, 931, 1112, 1134 |
| RsaNI | GTAC | 4 cut(s) | 831, 930, 1111, 1133 |
| RseI | CAYNNNNRTG | 3 cut(s) | 353, 560, 955 |
| SapI | GCTCTTC | 1 cut(s) | 1281 |
| SaqAI | TTAA | 2 cut(s) | 1043, 1367 |
| SatI | GCNGC | 4 cut(s) | 394, 493, 791, 794 |
| Sau96I | GGNCC | 4 cut(s) | 197, 960, 1212, 1348 |
| SchI | GAGTC | 4 cut(s) | 368, 626, 1093, 1440 |
| ScrFI | CCNGG | 2 cut(s) | 721, 1335 |
| SfaNI | GCATC | 2 cut(s) | 456, 1009 |
| SfcI | CTRYAG | 1 cut(s) | 1116 |
| SfoI | GGCGCC | 1 cut(s) | 718 |
| SfuI | TTCGAA | 2 cut(s) | 73, 1065 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 353, 560, 955 |
| SmlI | CTYRAG | 3 cut(s) | 261, 523, 745 |
| SmoI | CTYRAG | 3 cut(s) | 261, 523, 745 |
| SpeI | ACTAGT | 1 cut(s) | 1129 |
| Sse9I | AATT | 5 cut(s) | 470, 891, 1025, 1067, 1497 |
| SsiI | CCGC | 6 cut(s) | 88, 394, 405, 926, 1105, 1492 |
| SspDI | GGCGCC | 1 cut(s) | 716 |
| SspMI | CTAG | 3 cut(s) | 332, 1005, 1130 |
| StyD4I | CCNGG | 2 cut(s) | 719, 1333 |
| StyI | CCWWGG | 1 cut(s) | 331 |
| TaaI | ACNGT | 3 cut(s) | 302, 352, 627 |
| TaiI | ACGT | 4 cut(s) | 229, 502, 1179, 1357 |
| TaqI | TCGA | 6 cut(s) | 73, 166, 783, 1065, 1173, 1395 |
| TasI | AATT | 5 cut(s) | 470, 891, 1025, 1067, 1497 |
| TauI | GCSGC | 1 cut(s) | 396 |
| TfiI | GAWTC | 4 cut(s) | 863, 935, 1170, 1384 |
| Tru1I | TTAA | 2 cut(s) | 1043, 1367 |
| Tru9I | TTAA | 2 cut(s) | 1043, 1367 |
| TscAI | CASTG | 3 cut(s) | 383, 1417, 1476 |
| TseFI | GTSAC | 1 cut(s) | 1184 |
| TseI | GCWGC | 3 cut(s) | 492, 790, 793 |
| Tsp45I | GTSAC | 1 cut(s) | 1184 |
| TspDTI | ATGAA | 8 cut(s) | 31, 152, 237, 301, 773, 930, 1065, 1433 |
| TspRI | CASTG | 3 cut(s) | 383, 1417, 1476 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 131, 1357 |
| XapI | RAATTY | 1 cut(s) | 1025 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1358 |
| XmaJI | CCTAGG | 1 cut(s) | 331 |
| XmnI | GAANNNNTTC | 1 cut(s) | 764 |
| XspI | CTAG | 3 cut(s) | 332, 1005, 1130 |