Reverse transcriptase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 10 |
| AciI | CCGC | 2 cut(s) | 170, 239 |
| AclWI | GGATC | 2 cut(s) | 73, 197 |
| AcoI | YGGCCR | 1 cut(s) | 87 |
| AcsI | RAATTY | 1 cut(s) | 38 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 245, 280 |
| AgsI | TTSAA | 2 cut(s) | 130, 280 |
| AjnI | CCWGG | 1 cut(s) | 47 |
| AlwI | GGATC | 2 cut(s) | 73, 197 |
| Aor13HI | TCCGGA | 1 cut(s) | 10 |
| AoxI | GGCC | 4 cut(s) | 50, 87, 270, 282 |
| ApaI | GGGCCC | 1 cut(s) | 274 |
| ApeKI | GCWGC | 1 cut(s) | 261 |
| ApoI | RAATTY | 1 cut(s) | 38 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 284 |
| AspLEI | GCGC | 1 cut(s) | 196 |
| AspS9I | GGNCC | 3 cut(s) | 249, 270, 271 |
| AsuC2I | CCSGG | 1 cut(s) | 253 |
| AsuHPI | GGTGA | 1 cut(s) | 151 |
| AvaII | GGWCC | 1 cut(s) | 249 |
| BaeGI | GKGCMC | 1 cut(s) | 274 |
| BanII | GRGCYC | 1 cut(s) | 274 |
| BbvI | GCAGC | 1 cut(s) | 273 |
| BceAI | ACGGC | 1 cut(s) | 37 |
| BciT130I | CCWGG | 1 cut(s) | 49 |
| BcnI | CCSGG | 1 cut(s) | 253 |
| BfoI | RGCGCY | 1 cut(s) | 197 |
| BisI | GCNGC | 3 cut(s) | 171, 239, 262 |
| BlsI | GCNGC | 3 cut(s) | 172, 240, 263 |
| Bme1390I | CCNGG | 2 cut(s) | 49, 253 |
| Bme18I | GGWCC | 1 cut(s) | 249 |
| BmgT120I | GGNCC | 3 cut(s) | 249, 270, 271 |
| BmiI | GGNNCC | 3 cut(s) | 250, 271, 272 |
| BmrFI | CCNGG | 2 cut(s) | 49, 253 |
| BmrI | ACTGGG | 1 cut(s) | 268 |
| BmuI | ACTGGG | 1 cut(s) | 268 |
| BpmI | CTGGAG | 1 cut(s) | 31 |
| BpuMI | CCSGG | 1 cut(s) | 253 |
| Bsa29I | ATCGAT | 1 cut(s) | 295 |
| BsaJI | CCNNGG | 1 cut(s) | 206 |
| BsaWI | WCCGGW | 1 cut(s) | 10 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 245, 280 |
| Bse1I | ACTGG | 1 cut(s) | 274 |
| Bse3DI | GCAATG | 1 cut(s) | 180 |
| BseAI | TCCGGA | 1 cut(s) | 10 |
| BseBI | CCWGG | 1 cut(s) | 49 |
| BseCI | ATCGAT | 1 cut(s) | 295 |
| BseDI | CCNNGG | 1 cut(s) | 206 |
| BseGI | GGATG | 1 cut(s) | 68 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 245, 280 |
| BseMI | GCAATG | 1 cut(s) | 180 |
| BseNI | ACTGG | 1 cut(s) | 274 |
| BseRI | GAGGAG | 2 cut(s) | 32, 41 |
| BseSI | GKGCMC | 1 cut(s) | 274 |
| BseXI | GCAGC | 1 cut(s) | 273 |
| Bsh1285I | CGRYCG | 1 cut(s) | 299 |
| BshFI | GGCC | 4 cut(s) | 52, 89, 272, 284 |
| BshVI | ATCGAT | 1 cut(s) | 295 |
| BsiEI | CGRYCG | 1 cut(s) | 299 |
| BsiSI | CCGG | 3 cut(s) | 11, 33, 252 |
| BslFI | GGGAC | 3 cut(s) | 224, 262, 268 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 245, 280 |
| BsmFI | GGGAC | 3 cut(s) | 224, 262, 268 |
| BsnI | GGCC | 4 cut(s) | 52, 89, 272, 284 |
| Bsp120I | GGGCCC | 1 cut(s) | 270 |
| Bsp1286I | GDGCHC | 1 cut(s) | 274 |
| Bsp13I | TCCGGA | 1 cut(s) | 10 |
| Bsp143I | GATC | 4 cut(s) | 65, 202, 292, 296 |
| BspACI | CCGC | 2 cut(s) | 170, 239 |
| BspANI | GGCC | 4 cut(s) | 52, 89, 272, 284 |
| BspDI | ATCGAT | 1 cut(s) | 295 |
| BspEI | TCCGGA | 1 cut(s) | 10 |
| BspLI | GGNNCC | 3 cut(s) | 250, 271, 272 |
| BspPI | GGATC | 2 cut(s) | 73, 197 |
| BsrDI | GCAATG | 1 cut(s) | 180 |
| BsrI | ACTGG | 1 cut(s) | 274 |
| BssECI | CCNNGG | 1 cut(s) | 206 |
| BssMI | GATC | 4 cut(s) | 65, 202, 292, 296 |
| Bst2UI | CCWGG | 1 cut(s) | 49 |
| Bst6I | CTCTTC | 1 cut(s) | 9 |
| BstF5I | GGATG | 1 cut(s) | 68 |
| BstH2I | RGCGCY | 1 cut(s) | 197 |
| BstHHI | GCGC | 1 cut(s) | 196 |
| BstKTI | GATC | 4 cut(s) | 68, 205, 295, 299 |
| BstMBI | GATC | 4 cut(s) | 65, 202, 292, 296 |
| BstMCI | CGRYCG | 1 cut(s) | 299 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 102, 176 |
| BstNI | CCWGG | 1 cut(s) | 49 |
| BstSCI | CCNGG | 2 cut(s) | 47, 251 |
| BstSLI | GKGCMC | 1 cut(s) | 274 |
| BstV1I | GCAGC | 1 cut(s) | 273 |
| Bsu15I | ATCGAT | 1 cut(s) | 295 |
| BsuRI | GGCC | 4 cut(s) | 52, 89, 272, 284 |
| BsuTUI | ATCGAT | 1 cut(s) | 295 |
| BtsCI | GGATG | 1 cut(s) | 68 |
| BtsI | GCAGTG | 1 cut(s) | 271 |
| BtsIMutI | CAGTG | 1 cut(s) | 271 |
| CfoI | GCGC | 1 cut(s) | 196 |
| Cfr13I | GGNCC | 3 cut(s) | 249, 270, 271 |
| ClaI | ATCGAT | 1 cut(s) | 295 |
| CseI | GACGC | 2 cut(s) | 72, 85 |
| CviJI | RGCY | 6 cut(s) | 52, 89, 121, 238, 272, 284 |
| CviKI_1 | RGCY | 6 cut(s) | 52, 89, 121, 238, 272, 284 |
| DpnI | GATC | 4 cut(s) | 67, 204, 294, 298 |
| DpnII | GATC | 4 cut(s) | 65, 202, 292, 296 |
| EaeI | YGGCCR | 1 cut(s) | 87 |
| Eam1104I | CTCTTC | 1 cut(s) | 9 |
| EarI | CTCTTC | 1 cut(s) | 9 |
| Eco147I | AGGCCT | 1 cut(s) | 284 |
| Eco24I | GRGCYC | 1 cut(s) | 274 |
| Eco47I | GGWCC | 1 cut(s) | 249 |
| EcoO109I | RGGNCCY | 1 cut(s) | 270 |
| EcoRI | GAATTC | 1 cut(s) | 38 |
| EcoRII | CCWGG | 1 cut(s) | 47 |
| EcoT38I | GRGCYC | 1 cut(s) | 274 |
| FaiI | YATR | 1 cut(s) | 124 |
| FaqI | GGGAC | 3 cut(s) | 224, 262, 268 |
| Fnu4HI | GCNGC | 3 cut(s) | 171, 239, 262 |
| FokI | GGATG | 1 cut(s) | 55 |
| FriOI | GRGCYC | 1 cut(s) | 274 |
| Fsp4HI | GCNGC | 3 cut(s) | 171, 239, 262 |
| GlaI | GCGC | 1 cut(s) | 195 |
| GluI | GCNGC | 3 cut(s) | 171, 239, 262 |
| GsuI | CTGGAG | 1 cut(s) | 31 |
| HaeII | RGCGCY | 1 cut(s) | 197 |
| HaeIII | GGCC | 4 cut(s) | 52, 89, 272, 284 |
| HapII | CCGG | 3 cut(s) | 11, 33, 252 |
| HgaI | GACGC | 2 cut(s) | 72, 85 |
| HhaI | GCGC | 1 cut(s) | 196 |
| Hin6I | GCGC | 1 cut(s) | 194 |
| HinP1I | GCGC | 1 cut(s) | 194 |
| HinfI | GANTC | 2 cut(s) | 7, 29 |
| HpaII | CCGG | 3 cut(s) | 11, 33, 252 |
| HphI | GGTGA | 1 cut(s) | 151 |
| Hpy188I | TCNGA | 1 cut(s) | 207 |
| Hpy188III | TCNNGA | 2 cut(s) | 11, 232 |
| Hpy99I | CGWCG | 3 cut(s) | 79, 88, 101 |
| HpyAV | CCTTC | 5 cut(s) | 56, 154, 274, 295, 295 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 102, 176 |
| HspAI | GCGC | 1 cut(s) | 194 |
| Kpn2I | TCCGGA | 1 cut(s) | 10 |
| Kzo9I | GATC | 4 cut(s) | 65, 202, 292, 296 |
| LpnPI | CCDG | 9 cut(s) | 24, 34, 46, 61, 103, 165, 217, 265, 287 |
| Lsp1109I | GCAGC | 1 cut(s) | 273 |
| MalI | GATC | 4 cut(s) | 67, 204, 294, 298 |
| MboI | GATC | 4 cut(s) | 65, 202, 292, 296 |
| MboII | GAAGA | 2 cut(s) | 26, 34 |
| MhlI | GDGCHC | 1 cut(s) | 274 |
| MluCI | AATT | 3 cut(s) | 38, 183, 214 |
| MlyI | GAGTC | 1 cut(s) | 38 |
| MnlI | CCTC | 8 cut(s) | 10, 19, 82, 94, 139, 159, 201, 220 |
| MroI | TCCGGA | 1 cut(s) | 10 |
| MroXI | GAANNNNTTC | 1 cut(s) | 284 |
| MspA1I | CMGCKG | 1 cut(s) | 241 |
| MspI | CCGG | 3 cut(s) | 11, 33, 252 |
| MspR9I | CCNGG | 2 cut(s) | 49, 253 |
| MvaI | CCWGG | 1 cut(s) | 49 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 102, 176 |
| NciI | CCSGG | 1 cut(s) | 253 |
| NdeII | GATC | 4 cut(s) | 65, 202, 292, 296 |
| NlaIV | GGNNCC | 3 cut(s) | 250, 271, 272 |
| PceI | AGGCCT | 1 cut(s) | 284 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 80 |
| PdmI | GAANNNNTTC | 1 cut(s) | 284 |
| PfeI | GAWTC | 1 cut(s) | 7 |
| PkrI | GCNGC | 3 cut(s) | 172, 240, 263 |
| Ple19I | CGATCG | 1 cut(s) | 299 |
| PleI | GAGTC | 1 cut(s) | 37 |
| PpsI | GAGTC | 1 cut(s) | 37 |
| Psp6I | CCWGG | 1 cut(s) | 47 |
| PspGI | CCWGG | 1 cut(s) | 47 |
| PspN4I | GGNNCC | 3 cut(s) | 250, 271, 272 |
| PspOMI | GGGCCC | 1 cut(s) | 270 |
| PspPI | GGNCC | 3 cut(s) | 249, 270, 271 |
| PvuI | CGATCG | 1 cut(s) | 299 |
| SatI | GCNGC | 3 cut(s) | 171, 239, 262 |
| Sau3AI | GATC | 4 cut(s) | 65, 202, 292, 296 |
| Sau96I | GGNCC | 3 cut(s) | 249, 270, 271 |
| SchI | GAGTC | 1 cut(s) | 38 |
| ScrFI | CCNGG | 2 cut(s) | 49, 253 |
| SduI | GDGCHC | 1 cut(s) | 274 |
| SetI | ASST | 1 cut(s) | 231 |
| SinI | GGWCC | 1 cut(s) | 249 |
| Sse9I | AATT | 3 cut(s) | 38, 183, 214 |
| SseBI | AGGCCT | 1 cut(s) | 284 |
| SsiI | CCGC | 2 cut(s) | 170, 239 |
| StuI | AGGCCT | 1 cut(s) | 284 |
| StyD4I | CCNGG | 2 cut(s) | 47, 251 |
| TaqI | TCGA | 4 cut(s) | 99, 225, 295, 299 |
| TasI | AATT | 3 cut(s) | 38, 183, 214 |
| TauI | GCSGC | 2 cut(s) | 173, 241 |
| TfiI | GAWTC | 1 cut(s) | 7 |
| TscAI | CASTG | 1 cut(s) | 271 |
| TseI | GCWGC | 1 cut(s) | 261 |
| TspDTI | ATGAA | 1 cut(s) | 277 |
| TspRI | CASTG | 1 cut(s) | 271 |
| VpaK11BI | GGWCC | 1 cut(s) | 249 |
| XapI | RAATTY | 1 cut(s) | 38 |
| XmnI | GAANNNNTTC | 1 cut(s) | 284 |