E3 ubiquitin-protein ligase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 309 |
| AccII | CGCG | 1 cut(s) | 474 |
| AciI | CCGC | 2 cut(s) | 252, 472 |
| AcsI | RAATTY | 2 cut(s) | 120, 152 |
| AcyI | GRCGYC | 1 cut(s) | 310 |
| AdeI | CACNNNGTG | 1 cut(s) | 201 |
| AfaI | GTAC | 1 cut(s) | 70 |
| AflIII | ACRYGT | 2 cut(s) | 431, 489 |
| AgsI | TTSAA | 4 cut(s) | 35, 63, 283, 341 |
| AluBI | AGCT | 2 cut(s) | 173, 407 |
| AluI | AGCT | 2 cut(s) | 173, 407 |
| AoxI | GGCC | 1 cut(s) | 469 |
| ApoI | RAATTY | 2 cut(s) | 120, 152 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 372, 404 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 58 |
| AspLEI | GCGC | 1 cut(s) | 312 |
| AsuHPI | GGTGA | 2 cut(s) | 59, 153 |
| AsuII | TTCGAA | 1 cut(s) | 320 |
| BanI | GGYRCC | 1 cut(s) | 309 |
| BbsI | GAAGAC | 1 cut(s) | 42 |
| BccI | CCATC | 1 cut(s) | 134 |
| BciVI | GTATCC | 1 cut(s) | 7 |
| BfaI | CTAG | 2 cut(s) | 170, 539 |
| BfoI | RGCGCY | 1 cut(s) | 313 |
| BfuI | GTATCC | 1 cut(s) | 7 |
| BisI | GCNGC | 1 cut(s) | 472 |
| BlsI | GCNGC | 1 cut(s) | 473 |
| BmiI | GGNNCC | 1 cut(s) | 311 |
| BpiI | GAAGAC | 1 cut(s) | 42 |
| Bpu14I | TTCGAA | 1 cut(s) | 320 |
| Bsa29I | ATCGAT | 1 cut(s) | 138 |
| BsaHI | GRCGYC | 1 cut(s) | 310 |
| BsaJI | CCNNGG | 1 cut(s) | 242 |
| Bse1I | ACTGG | 1 cut(s) | 313 |
| BseCI | ATCGAT | 1 cut(s) | 138 |
| BseDI | CCNNGG | 1 cut(s) | 242 |
| BseNI | ACTGG | 1 cut(s) | 313 |
| BsgI | GTGCAG | 1 cut(s) | 424 |
| Bsh1236I | CGCG | 1 cut(s) | 474 |
| BshFI | GGCC | 1 cut(s) | 471 |
| BshNI | GGYRCC | 1 cut(s) | 309 |
| BshVI | ATCGAT | 1 cut(s) | 138 |
| BslFI | GGGAC | 1 cut(s) | 464 |
| BsmFI | GGGAC | 1 cut(s) | 464 |
| BsnI | GGCC | 1 cut(s) | 471 |
| Bsp119I | TTCGAA | 1 cut(s) | 320 |
| Bsp143I | GATC | 3 cut(s) | 49, 183, 444 |
| BspACI | CCGC | 2 cut(s) | 252, 472 |
| BspANI | GGCC | 1 cut(s) | 471 |
| BspDI | ATCGAT | 1 cut(s) | 138 |
| BspFNI | CGCG | 1 cut(s) | 474 |
| BspLI | GGNNCC | 1 cut(s) | 311 |
| BspT104I | TTCGAA | 1 cut(s) | 320 |
| BspT107I | GGYRCC | 1 cut(s) | 309 |
| BsrI | ACTGG | 1 cut(s) | 313 |
| BssECI | CCNNGG | 1 cut(s) | 242 |
| BssMI | GATC | 3 cut(s) | 49, 183, 444 |
| BssNI | GRCGYC | 1 cut(s) | 310 |
| Bst4CI | ACNGT | 1 cut(s) | 199 |
| BstACI | GRCGYC | 1 cut(s) | 310 |
| BstBI | TTCGAA | 1 cut(s) | 320 |
| BstC8I | GCNNGC | 2 cut(s) | 92, 426 |
| BstFNI | CGCG | 1 cut(s) | 474 |
| BstH2I | RGCGCY | 1 cut(s) | 313 |
| BstHHI | GCGC | 1 cut(s) | 312 |
| BstKTI | GATC | 3 cut(s) | 52, 186, 447 |
| BstMBI | GATC | 3 cut(s) | 49, 183, 444 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 96 |
| BstNSI | RCATGY | 3 cut(s) | 428, 435, 493 |
| BstUI | CGCG | 1 cut(s) | 474 |
| BstV2I | GAAGAC | 1 cut(s) | 42 |
| Bsu15I | ATCGAT | 1 cut(s) | 138 |
| BsuI | GTATCC | 1 cut(s) | 7 |
| BsuRI | GGCC | 1 cut(s) | 471 |
| BsuTUI | ATCGAT | 1 cut(s) | 138 |
| BtsIMutI | CAGTG | 1 cut(s) | 320 |
| Cac8I | GCNNGC | 2 cut(s) | 92, 426 |
| CfoI | GCGC | 1 cut(s) | 312 |
| ClaI | ATCGAT | 1 cut(s) | 138 |
| Csp6I | GTAC | 1 cut(s) | 69 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 350, 385 |
| CviAII | CATG | 5 cut(s) | 100, 259, 425, 432, 490 |
| CviJI | RGCY | 3 cut(s) | 173, 407, 471 |
| CviKI_1 | RGCY | 3 cut(s) | 173, 407, 471 |
| CviQI | GTAC | 1 cut(s) | 69 |
| DinI | GGCGCC | 1 cut(s) | 311 |
| DpnI | GATC | 3 cut(s) | 51, 185, 446 |
| DpnII | GATC | 3 cut(s) | 49, 183, 444 |
| DraIII | CACNNNGTG | 1 cut(s) | 201 |
| EgeI | GGCGCC | 1 cut(s) | 311 |
| EheI | GGCGCC | 1 cut(s) | 311 |
| FaeI | CATG | 5 cut(s) | 103, 262, 428, 435, 493 |
| FaqI | GGGAC | 1 cut(s) | 464 |
| FatI | CATG | 5 cut(s) | 99, 258, 424, 431, 489 |
| FauNDI | CATATG | 1 cut(s) | 333 |
| Fnu4HI | GCNGC | 1 cut(s) | 472 |
| Fsp4HI | GCNGC | 1 cut(s) | 472 |
| FspBI | CTAG | 2 cut(s) | 170, 539 |
| GlaI | GCGC | 1 cut(s) | 311 |
| GluI | GCNGC | 1 cut(s) | 472 |
| HaeII | RGCGCY | 1 cut(s) | 313 |
| HaeIII | GGCC | 1 cut(s) | 471 |
| HhaI | GCGC | 1 cut(s) | 312 |
| Hin1I | GRCGYC | 1 cut(s) | 310 |
| Hin1II | CATG | 5 cut(s) | 103, 262, 428, 435, 493 |
| Hin6I | GCGC | 1 cut(s) | 310 |
| HinP1I | GCGC | 1 cut(s) | 310 |
| HincII | GTYRAC | 1 cut(s) | 77 |
| HindII | GTYRAC | 1 cut(s) | 77 |
| HinfI | GANTC | 1 cut(s) | 192 |
| HphI | GGTGA | 2 cut(s) | 59, 153 |
| Hpy166II | GTNNAC | 3 cut(s) | 77, 229, 505 |
| Hpy188I | TCNGA | 1 cut(s) | 243 |
| Hpy188III | TCNNGA | 1 cut(s) | 539 |
| Hpy8I | GTNNAC | 3 cut(s) | 77, 229, 505 |
| Hpy99I | CGWCG | 2 cut(s) | 479, 482 |
| HpyAV | CCTTC | 1 cut(s) | 4 |
| HpyCH4III | ACNGT | 1 cut(s) | 199 |
| HpyCH4V | TGCA | 6 cut(s) | 99, 293, 327, 372, 441, 533 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 96 |
| Hsp92I | GRCGYC | 1 cut(s) | 310 |
| Hsp92II | CATG | 5 cut(s) | 103, 262, 428, 435, 493 |
| HspAI | GCGC | 1 cut(s) | 310 |
| KasI | GGCGCC | 1 cut(s) | 309 |
| Kzo9I | GATC | 3 cut(s) | 49, 183, 444 |
| LmnI | GCTCC | 1 cut(s) | 404 |
| LpnPI | CCDG | 6 cut(s) | 30, 177, 225, 326, 433, 451 |
| MaeI | CTAG | 2 cut(s) | 170, 539 |
| MaeIII | GTNAC | 3 cut(s) | 159, 214, 485 |
| MalI | GATC | 3 cut(s) | 51, 185, 446 |
| MboI | GATC | 3 cut(s) | 49, 183, 444 |
| MboII | GAAGA | 3 cut(s) | 47, 310, 427 |
| MfeI | CAATTG | 2 cut(s) | 63, 275 |
| MluCI | AATT | 6 cut(s) | 24, 63, 120, 152, 275, 520 |
| Mly113I | GGCGCC | 1 cut(s) | 310 |
| MnlI | CCTC | 1 cut(s) | 237 |
| MroXI | GAANNNNTTC | 1 cut(s) | 58 |
| MseI | TTAA | 1 cut(s) | 23 |
| MunI | CAATTG | 2 cut(s) | 63, 275 |
| MvnI | CGCG | 1 cut(s) | 474 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 96 |
| NarI | GGCGCC | 1 cut(s) | 310 |
| NdeI | CATATG | 1 cut(s) | 333 |
| NdeII | GATC | 3 cut(s) | 49, 183, 444 |
| NlaIII | CATG | 5 cut(s) | 103, 262, 428, 435, 493 |
| NlaIV | GGNNCC | 1 cut(s) | 311 |
| NmuCI | GTSAC | 2 cut(s) | 159, 485 |
| NspI | RCATGY | 3 cut(s) | 428, 435, 493 |
| NspV | TTCGAA | 1 cut(s) | 320 |
| PaeI | GCATGC | 1 cut(s) | 428 |
| PciI | ACATGT | 2 cut(s) | 431, 489 |
| PdmI | GAANNNNTTC | 1 cut(s) | 58 |
| PfeI | GAWTC | 1 cut(s) | 192 |
| PkrI | GCNGC | 1 cut(s) | 473 |
| PluTI | GGCGCC | 1 cut(s) | 313 |
| PscI | ACATGT | 2 cut(s) | 431, 489 |
| PspN4I | GGNNCC | 1 cut(s) | 311 |
| RsaI | GTAC | 1 cut(s) | 70 |
| RsaNI | GTAC | 1 cut(s) | 69 |
| SaqAI | TTAA | 1 cut(s) | 23 |
| SatI | GCNGC | 1 cut(s) | 472 |
| Sau3AI | GATC | 3 cut(s) | 49, 183, 444 |
| SetI | ASST | 4 cut(s) | 166, 175, 248, 409 |
| SfoI | GGCGCC | 1 cut(s) | 311 |
| SfuI | TTCGAA | 1 cut(s) | 320 |
| SphI | GCATGC | 1 cut(s) | 428 |
| Sse9I | AATT | 6 cut(s) | 24, 63, 120, 152, 275, 520 |
| SsiI | CCGC | 2 cut(s) | 252, 472 |
| SspDI | GGCGCC | 1 cut(s) | 309 |
| SspI | AATATT | 1 cut(s) | 86 |
| SspMI | CTAG | 2 cut(s) | 170, 539 |
| TaaI | ACNGT | 1 cut(s) | 199 |
| TaqI | TCGA | 5 cut(s) | 52, 138, 186, 320, 518 |
| TasI | AATT | 6 cut(s) | 24, 63, 120, 152, 275, 520 |
| TauI | GCSGC | 1 cut(s) | 474 |
| TfiI | GAWTC | 1 cut(s) | 192 |
| Tru1I | TTAA | 1 cut(s) | 23 |
| Tru9I | TTAA | 1 cut(s) | 23 |
| TscAI | CASTG | 1 cut(s) | 320 |
| TseFI | GTSAC | 2 cut(s) | 159, 485 |
| Tsp45I | GTSAC | 2 cut(s) | 159, 485 |
| TspDTI | ATGAA | 1 cut(s) | 116 |
| TspRI | CASTG | 1 cut(s) | 320 |
| XapI | RAATTY | 2 cut(s) | 120, 152 |
| XbaI | TCTAGA | 1 cut(s) | 538 |
| XceI | RCATGY | 3 cut(s) | 428, 435, 493 |
| XmnI | GAANNNNTTC | 1 cut(s) | 58 |
| XspI | CTAG | 2 cut(s) | 170, 539 |