pycom13g23800

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000533
Physical Location & Seq
Reverse (-)
21183090 .. 21185176
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
ATGTATAACAAGTTCTTGGCTATAGAAAACAAAACGTCCAGTTCTAAGTGGAAGGAAAAAAAACGAATATCTGACTTCAAAAACATGAAGAAATCAACAATATATTCAATACTATCAGTGTTGCACATCTTTGCTCTTCATCTTCAATCAGAGGTTAACTCGTTCGCTACTACTCGTGATCATGATTTTCGCTACATGAGATTTGTGTATAACGCCAATGATCCACGGTTAGAAGCATCGTATGACTATATTGTCATTGGGGGAGGAACAGCAGGTTGCCCACTAGCAGCAACTTTATCAGCAAACTACTCAGTGCTCCTTCTAGAAAGGGGCAGTATTCCTACAGCATATCCGAGCGTCTTGCGTGACGATGGGTTTCTAACTACTCTAATGCGGGAAGATAATGGCGAGACACCAGCTCAAAAGTTCACATCAGAAGATGGTGTTCCTAATGTCAGAGGCAGGGTCTTAGGTGGGTCAAGCATGATTAATGCTGGCTTCTACACCAGAGCTGACAGGGATTTCTTTATGAAATCAGGGATTGAGTGGAACATGGATTCGGTTTATAAGGCATATCGGTGGGTTGAAGAAACTATTGTGTTCCGCCCAACTATGGATCCCTGGCAATCTGCTGTAGCAAGAGCATTGTTGGAGGCTGGTGTTGGTCCAGACAATGGACTTAATTTGGATCACATTAAAGGAACTAAAATTGGGGGTTCGACGTTTGACAACAATGGGAGAAGACATGGAGCTGTGGAGCTGCTTAATAAGGGAGATCCGAACAAATTGCGAGTTGCGGTTCATGCCACAGTAGAGAACGATCCTCTTCTCTTCCAAGGCATCAAGTTTGTCAGCTATTGGAATCATGTATAG

Protein Analysis

291

Amino Acids

32.57

Weight (kDa)

8.78

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 81 - 220 1.6e-06 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000533)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08950 FvH4_6g09340
malus_domestica MD00G1012800.v1.1 MD00G1012900.v1.1 MD00G1013000.v1.1 MD00G1013300.v1.1 MD03G1090900.v1.1 MD03G1091100.v1.1 MD03G1091300.v1.1 MD03G1091400.v1.1 MD03G1091700.v1.1 MD03G1091900.v1.1 MD10G1279300.v1.1 MD13G1264000.v1.1 MD13G1264100.v1.1 MD13G1264200.v1.1 MD13G1264500.v1.1 MD16G1093000.v1.1 MD16G1093100.v1.1 MD16G1265300.v1.1 MD16G1265500.v1.1
prunus_persica Prupe.1G007400_v2.0.a1 Prupe.1G092200_v2.0.a1 Prupe.1G092300_v2.0.a1 Prupe.1G092400_v2.0.a1 Prupe.1G092500_v2.0.a1 Prupe.1G092700_v2.0.a1 Prupe.1G092900_v2.0.a1 Prupe.1G093000_v2.0.a1 Prupe.1G093200_v2.0.a1 Prupe.1G093300_v2.0.a1 Prupe.1G093500_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093600_v2.0.a1 Prupe.1G093700_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G093800_v2.0.a1 Prupe.1G096400_v2.0.a1 Prupe.1G096500_v2.0.a1 Prupe.1G096500_v2.0.a1
pyrus_communis pycom03g07290 pycom03g07320 pycom10g23300 pycom11g08780 pycom11g08790 pycom11g08810 pycom11g08820 pycom13g23800
rosa_chinensis RchiOBHm_Chr4g0403711 RchiOBHm_Chr4g0403741 RchiOBHm_Chr4g0403781
rosa_laevigata RLG00000008950 RLG00000008955 RLG00000008957 RLG00000008959
rosa_multiflora Rmu_co8470309.1_g000001 Rmu_sc0001496.1_g000015 Rmu_sc0004115.1_g000016 Rmu_sc0004388.1_g000007 Rmu_sc0007461.1_g000001 Rmu_sc0010412.1_g000002
rosa_roxburghii Rroxscaffold_5G00348330 Rroxscaffold_5G00348340 Rroxscaffold_5G00348350 Rroxscaffold_5G00348400
rosa_rugosa Rorug04G0045400 Rorug04G0045500 Rorug04G0045600
rosa_samantha Rh4AG120800 Rh4AG121000 Rh4BG113600 Rh4BG113900 Rh4BG114000 Rh4CG128300 Rh4CG129200 Rh4CG129300 Rh4CG129400 Rh4DG113700 Rh4DG113800 Rh4DG113900
rosa_wichuraiana Rw0G001200 Rw4G009830 Rw4G009850 Rw4G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 567
AasI GACNNNNNNGTC 1 cut(s) 251
Acc36I ACCTGC 1 cut(s) 263
AccB7I CCANNNNNTGG 1 cut(s) 674
AciI CCGC 3 cut(s) 394, 604, 797
AclWI GGATC 6 cut(s) 215, 611, 624, 696, 770, 815
AfiI CCNNNNNNNGG 2 cut(s) 613, 674
AgsI TTSAA 4 cut(s) 79, 108, 146, 587
AjnI CCWGG 1 cut(s) 620
AluBI AGCT 5 cut(s) 419, 512, 752, 760, 855
AluI AGCT 5 cut(s) 419, 512, 752, 760, 855
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 1 cut(s) 404
AlwI GGATC 6 cut(s) 215, 611, 624, 696, 770, 815
ApeKI GCWGC 2 cut(s) 287, 760
AseI ATTAAT 1 cut(s) 489
AspS9I GGNCC 1 cut(s) 665
AvaII GGWCC 1 cut(s) 665
BamHI GGATCC 1 cut(s) 616
BauI CACGAG 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 748
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 2 cut(s) 299, 747
BccI CCATC 2 cut(s) 365, 434
BcgI CGANNNNNNTGC 4 cut(s) 343, 377, 769, 803
BciT130I CCWGG 1 cut(s) 622
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 2 cut(s) 284, 323
BfmI CTRYAG 3 cut(s) 21, 342, 633
BfuAI ACCTGC 1 cut(s) 263
BisI GCNGC 2 cut(s) 288, 761
BlsI GCNGC 2 cut(s) 289, 762
Bme1390I CCNGG 1 cut(s) 622
Bme18I GGWCC 1 cut(s) 665
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 1 cut(s) 618
BmrFI CCNGG 1 cut(s) 622
BmsI GCATC 2 cut(s) 245, 849
BpiI GAAGAC 1 cut(s) 748
BplI GAGNNNNNCTC 2 cut(s) 143, 175
BsaJI CCNNGG 3 cut(s) 224, 620, 835
Bsc4I CCNNNNNNNGG 2 cut(s) 613, 674
Bse1I ACTGG 1 cut(s) 39
BseBI CCWGG 1 cut(s) 622
BseDI CCNNGG 3 cut(s) 224, 620, 835
BseLI CCNNNNNNNGG 2 cut(s) 613, 674
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 39
BseXI GCAGC 2 cut(s) 299, 747
BsiHKAI GWGCWC 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 613, 674
BsmAI GTCTC 1 cut(s) 404
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 6 cut(s) 178, 220, 616, 688, 775, 820
BspACI CCGC 3 cut(s) 394, 604, 797
BspCNI CTCAG 1 cut(s) 323
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 618
BspMI ACCTGC 1 cut(s) 263
BspPI GGATC 6 cut(s) 215, 611, 624, 696, 770, 815
BspQI GCTCTTC 1 cut(s) 141
BsrI ACTGG 1 cut(s) 39
BssECI CCNNGG 3 cut(s) 224, 620, 835
BssMI GATC 6 cut(s) 178, 220, 616, 688, 775, 820
BssSI CACGAG 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 835
Bst2BI CACGAG 1 cut(s) 174
Bst2UI CCWGG 1 cut(s) 622
Bst4CI ACNGT 2 cut(s) 228, 811
Bst6I CTCTTC 3 cut(s) 141, 831, 836
BstC8I GCNNGC 1 cut(s) 496
BstDEI CTNAG 3 cut(s) 45, 310, 469
BstDSI CCRYGG 1 cut(s) 224
BstKTI GATC 6 cut(s) 181, 223, 619, 691, 778, 823
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 6 cut(s) 178, 220, 616, 688, 775, 820
BstMWI GCNNNNNNNGC 1 cut(s) 803
BstNI CCWGG 1 cut(s) 622
BstSCI CCNGG 1 cut(s) 620
BstSFI CTRYAG 3 cut(s) 21, 342, 633
BstV1I GCAGC 2 cut(s) 299, 747
BstV2I GAAGAC 1 cut(s) 748
BstX2I RGATCY 2 cut(s) 616, 775
BstYI RGATCY 2 cut(s) 616, 775
BtgI CCRYGG 1 cut(s) 224
BtsIMutI CAGTG 2 cut(s) 123, 318
BveI ACCTGC 1 cut(s) 263
Cac8I GCNNGC 1 cut(s) 496
CciI TCATGA 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 665
CseI GACGC 1 cut(s) 346
CviAII CATG 8 cut(s) 85, 182, 196, 484, 553, 746, 803, 866
CviJI RGCY 8 cut(s) 20, 419, 498, 512, 656, 752, 760, 855
CviKI_1 RGCY 8 cut(s) 20, 419, 498, 512, 656, 752, 760, 855
DdeI CTNAG 3 cut(s) 45, 310, 469
DpnI GATC 6 cut(s) 180, 222, 618, 690, 777, 822
DpnII GATC 6 cut(s) 178, 220, 616, 688, 775, 820
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 3 cut(s) 141, 831, 836
EarI CTCTTC 3 cut(s) 141, 831, 836
EciI GGCGGA 1 cut(s) 593
Eco130I CCWWGG 1 cut(s) 835
Eco47I GGWCC 1 cut(s) 665
EcoRII CCWGG 1 cut(s) 620
EcoT14I CCWWGG 1 cut(s) 835
ErhI CCWWGG 1 cut(s) 835
FaeI CATG 8 cut(s) 88, 185, 199, 487, 556, 749, 806, 869
FatI CATG 8 cut(s) 84, 181, 195, 483, 552, 745, 802, 865
FauI CCCGC 1 cut(s) 387
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 2 cut(s) 288, 761
Fsp4HI GCNGC 2 cut(s) 288, 761
FspBI CTAG 2 cut(s) 284, 323
GluI GCNGC 2 cut(s) 288, 761
HgaI GACGC 1 cut(s) 346
Hin1II CATG 8 cut(s) 88, 185, 199, 487, 556, 749, 806, 869
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HinfI GANTC 2 cut(s) 557, 862
HpaI GTTAAC 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 157, 429
Hpy188I TCNGA 6 cut(s) 73, 151, 354, 436, 458, 780
Hpy188III TCNNGA 4 cut(s) 176, 182, 323, 668
Hpy8I GTNNAC 2 cut(s) 157, 429
Hpy99I CGWCG 1 cut(s) 724
HpyAV CCTTC 2 cut(s) 46, 329
HpyCH4III ACNGT 2 cut(s) 228, 811
HpyCH4IV ACGT 2 cut(s) 35, 722
HpyCH4V TGCA 1 cut(s) 124
HpyF10VI GCNNNNNNNGC 1 cut(s) 803
HpyF3I CTNAG 3 cut(s) 45, 310, 469
HpySE526I ACGT 2 cut(s) 35, 722
Hsp92II CATG 8 cut(s) 88, 185, 199, 487, 556, 749, 806, 869
Ksp22I TGATCA 1 cut(s) 178
KspAI GTTAAC 1 cut(s) 157
Kzo9I GATC 6 cut(s) 178, 220, 616, 688, 775, 820
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 3 cut(s) 321, 749, 757
Lsp1109I GCAGC 2 cut(s) 299, 747
LweI GCATC 2 cut(s) 245, 849
MaeI CTAG 2 cut(s) 284, 323
MaeII ACGT 2 cut(s) 35, 722
MaeIII GTNAC 1 cut(s) 365
MalI GATC 6 cut(s) 180, 222, 618, 690, 777, 822
MboI GATC 6 cut(s) 178, 220, 616, 688, 775, 820
MboII GAAGA 9 cut(s) 100, 128, 134, 410, 449, 599, 753, 818, 823
MflI RGATCY 2 cut(s) 616, 775
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 3 cut(s) 682, 708, 785
MmeI TCCRAC 1 cut(s) 630
MnlI CCTC 5 cut(s) 145, 257, 452, 646, 834
MseI TTAA 5 cut(s) 156, 489, 681, 696, 765
MslI CAYNNNNRTG 1 cut(s) 577
MspR9I CCNGG 1 cut(s) 622
MvaI CCWGG 1 cut(s) 622
MwoI GCNNNNNNNGC 1 cut(s) 803
NdeII GATC 6 cut(s) 178, 220, 616, 688, 775, 820
NlaIII CATG 8 cut(s) 88, 185, 199, 487, 556, 749, 806, 869
NlaIV GGNNCC 1 cut(s) 618
NmuCI GTSAC 1 cut(s) 365
PagI TCATGA 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 141
PfeI GAWTC 2 cut(s) 557, 862
PflMI CCANNNNNTGG 1 cut(s) 674
PkrI GCNGC 2 cut(s) 289, 762
PshBI ATTAAT 1 cut(s) 489
PsiI TTATAA 1 cut(s) 567
Psp6I CCWGG 1 cut(s) 620
PspGI CCWGG 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 618
PspPI GGNCC 1 cut(s) 665
PsrI GAACNNNNNNTAC 1 cut(s) 28
PsuI RGATCY 2 cut(s) 616, 775
RseI CAYNNNNRTG 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 5 cut(s) 156, 489, 681, 696, 765
SatI GCNGC 2 cut(s) 288, 761
Sau3AI GATC 6 cut(s) 178, 220, 616, 688, 775, 820
Sau96I GGNCC 1 cut(s) 665
ScrFI CCNGG 1 cut(s) 622
SduI GDGCHC 1 cut(s) 318
SfaNI GCATC 2 cut(s) 245, 849
SfcI CTRYAG 3 cut(s) 21, 342, 633
SinI GGWCC 1 cut(s) 665
SmiMI CAYNNNNRTG 1 cut(s) 577
Sse9I AATT 3 cut(s) 682, 708, 785
SsiI CCGC 3 cut(s) 394, 604, 797
SspMI CTAG 2 cut(s) 284, 323
StyD4I CCNGG 1 cut(s) 620
StyI CCWWGG 1 cut(s) 835
TaaI ACNGT 2 cut(s) 228, 811
TaiI ACGT 2 cut(s) 38, 725
TaqI TCGA 1 cut(s) 719
TasI AATT 3 cut(s) 682, 708, 785
TfiI GAWTC 2 cut(s) 557, 862
Tru1I TTAA 5 cut(s) 156, 489, 681, 696, 765
Tru9I TTAA 5 cut(s) 156, 489, 681, 696, 765
TscAI CASTG 2 cut(s) 123, 318
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 2 cut(s) 287, 760
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 4 cut(s) 101, 128, 545, 791
TspRI CASTG 2 cut(s) 123, 318
Van91I CCANNNNNTGG 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 665
VspI ATTAAT 1 cut(s) 489
XbaI TCTAGA 1 cut(s) 322
XspI CTAG 2 cut(s) 284, 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.