RchiOBHm_Chr4g0434691

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001259
Physical Location & Seq
Forward (+)
58400137 .. 58406046
5910 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40314

Sequence Viewer

Length: 1242 bp
ATGCTTGACTGTGGTCTACGAGAGAACTTTGTAGCAAACAAAGTTGTGGATTATTTTCAATTACCAACAGTGAAGCTGAGTGATCCATATTGGATTCCATTTGGAGAGGATGAATATGCACAAGTAACGGAGCTGAGTGATCCATACTGGATTCCATTTGGAGAGGATGAATATGCACAAGTAATAGAGGTTTGGAAAGTTCCTGTCTCTATGGGAAAATTTTATAAAGAAGAGATTACTTGTCATGTGATTGACATGGACGACACTAATGTGCTTTTTGGAAGACCATGGCATGAAAGCGTCAATGGTGGTTATTGCAAGGACAACACATATTTATTTAGGTGGTATGCGCACAAAATTAAAATCAAGCCTAGAAGAAAGAACATCAAGAATGACCATCTTGAGATGTGTGAAAAGAAACATGAAGAAGCCACTTTGAAAATTGAAGAGAAACTTATTAAAGACAAGGAAGATGATTCATTCATCACATCGATATCCATGTCATGCATGCTTAATAGTGTTCAAGACCAACCCAAAGAGAAGTCAATGACCATTCCTTTTCAAGTGGAGGAGCTTAAGTTTCAAGATGCTTATTCTAAACTTGTCTACGGTATTGGATTCATGGTGATACCTTTTAATTTCAAGAATATTGAAGTTGATGGCTTATCATGTTTTGTTCCTACTAATGATAGAGTTGCATTCAAGAGGAACTCGAGGTCGAGTTCTTTTCAAGTGGAGGAGACTGATGTAAGACGAGATTTCAGCCAATTTCAACAAAGAATCTCGAAAAGAAAGAAGCGTTTCTACATTAAGAAGAATGATTTGAATATTGGAAATTGGTATTCAAATAGGCTTCTTAATGATGAAATTAATCATAAATTACTCTTTTGGATATATCGGGATTTTCGAGCAAAATCTTGGTTGGGATTCCAAATACTTATTTGCTTAATATTCTTTATGGTGGTGGCTCCGGTTTTGGATTATGCCACTAACAATCGGTGGGGTAGTGAAGAGGTGTTAAGTAGAGGGTTTATGTTGAAATGGCTTGCTAGCAACTGCAGCCGGCGCGAGCTCAGCGGAGGGAAGTGTGGCTTCGATTTCAAGACGTCTCGTTTTAGCTGTTTGTGCCCAGATAGGCCTCATTCTGTCAGCTGTGAAGGTGGTCTTGTGATTCTAGTATTGGTTGTTGTTTGCTGCTTCAGGAGAAAGTTGTGTGATAAATCTACTACTTCTTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

413

Amino Acids

48.47

Weight (kDa)

8.24

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAK_assoc PF14380 331 - 379 3.7e-10 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 225
AatII GACGTC 1 cut(s) 1109
Acc16I TGCGCA 1 cut(s) 351
AccI GTMKAC 2 cut(s) 16, 606
AccII CGCG 1 cut(s) 1068
AciI CCGC 1 cut(s) 1077
AclWI GGATC 2 cut(s) 77, 134
AcsI RAATTY 1 cut(s) 218
AcuI CTGAAG 1 cut(s) 1183
AcyI GRCGYC 1 cut(s) 1106
AflII CTTAAG 1 cut(s) 575
AleI CACNNNNGTG 1 cut(s) 269
AluBI AGCT 6 cut(s) 76, 133, 574, 1072, 1119, 1152
AluI AGCT 6 cut(s) 76, 133, 574, 1072, 1119, 1152
Alw21I GWGCWC 1 cut(s) 1074
Alw26I GTCTC 3 cut(s) 211, 734, 1113
AlwI GGATC 2 cut(s) 77, 134
Ama87I CYCGRG 1 cut(s) 712
AoxI GGCC 1 cut(s) 1136
ApeKI GCWGC 2 cut(s) 1059, 1194
ApoI RAATTY 1 cut(s) 218
AseI ATTAAT 1 cut(s) 870
Asp700I GAANNNNTTC 1 cut(s) 800
AspLEI GCGC 2 cut(s) 352, 1068
AsuHPI GGTGA 1 cut(s) 637
AsuNHI GCTAGC 1 cut(s) 1049
AvaI CYCGRG 1 cut(s) 712
BaeGI GKGCMC 1 cut(s) 1130
BanII GRGCYC 1 cut(s) 1074
BarI GAAGNNNNNNTAC 2 cut(s) 788, 820
BbsI GAAGAC 1 cut(s) 289
Bbv12I GWGCWC 1 cut(s) 1074
BbvI GCAGC 2 cut(s) 1071, 1181
BccI CCATC 2 cut(s) 405, 653
BcoDI GTCTC 3 cut(s) 211, 734, 1113
BfaI CTAG 3 cut(s) 372, 1050, 1175
BfmI CTRYAG 1 cut(s) 1057
BfrI CTTAAG 1 cut(s) 575
BisI GCNGC 2 cut(s) 1060, 1195
BlpI GCTNAGC 1 cut(s) 1073
BlsI GCNGC 2 cut(s) 1061, 1196
BmeT110I CYCGRG 1 cut(s) 712
BmiI GGNNCC 1 cut(s) 969
BmsI GCATC 1 cut(s) 577
BmtI GCTAGC 1 cut(s) 1053
BoxI GACNNNNGTC 1 cut(s) 12
BpiI GAAGAC 1 cut(s) 289
Bpu1102I GCTNAGC 1 cut(s) 1073
BpuEI CTTGAG 1 cut(s) 422
Bsa29I ATCGAT 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 1106
BsaJI CCNNGG 1 cut(s) 287
BsaWI WCCGGW 1 cut(s) 970
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bse118I RCCGGY 1 cut(s) 1062
Bse1I ACTGG 1 cut(s) 152
BseCI ATCGAT 1 cut(s) 491
BseDI CCNNGG 1 cut(s) 287
BseGI GGATG 2 cut(s) 115, 172
BseMII CTCAG 3 cut(s) 68, 125, 1087
BseNI ACTGG 1 cut(s) 152
BseRI GAGGAG 2 cut(s) 584, 752
BseSI GKGCMC 1 cut(s) 1130
BseXI GCAGC 2 cut(s) 1071, 1181
Bsh1236I CGCG 1 cut(s) 1068
BshFI GGCC 1 cut(s) 1138
BshVI ATCGAT 1 cut(s) 491
BsiHKAI GWGCWC 1 cut(s) 1074
BsiHKCI CYCGRG 1 cut(s) 712
BsiSI CCGG 2 cut(s) 971, 1063
BsmAI GTCTC 3 cut(s) 211, 734, 1113
BsmBI CGTCTC 1 cut(s) 1113
BsmI GAATGC 1 cut(s) 698
BsnI GGCC 1 cut(s) 1138
BsoBI CYCGRG 1 cut(s) 712
Bsp1286I GDGCHC 2 cut(s) 1074, 1130
Bsp143I GATC 2 cut(s) 82, 139
Bsp1720I GCTNAGC 1 cut(s) 1073
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 1 cut(s) 1077
BspANI GGCC 1 cut(s) 1138
BspCNI CTCAG 3 cut(s) 69, 126, 1086
BspDI ATCGAT 1 cut(s) 491
BspFNI CGCG 1 cut(s) 1068
BspLI GGNNCC 1 cut(s) 969
BspMAI CTGCAG 1 cut(s) 1061
BspOI GCTAGC 1 cut(s) 1053
BspPI GGATC 2 cut(s) 77, 134
BspTI CTTAAG 1 cut(s) 575
BsrFI RCCGGY 1 cut(s) 1062
BsrI ACTGG 1 cut(s) 152
BssAI RCCGGY 1 cut(s) 1062
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 2 cut(s) 82, 139
BssNI GRCGYC 1 cut(s) 1106
BssT1I CCWWGG 1 cut(s) 287
Bst4CI ACNGT 3 cut(s) 11, 70, 611
Bst6I CTCTTC 3 cut(s) 225, 441, 1005
BstACI GRCGYC 1 cut(s) 1106
BstAFI CTTAAG 1 cut(s) 575
BstC8I GCNNGC 5 cut(s) 509, 1047, 1051, 1064, 1070
BstDEI CTNAG 3 cut(s) 77, 134, 1073
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 2 cut(s) 115, 172
BstFNI CGCG 1 cut(s) 1068
BstHHI GCGC 2 cut(s) 352, 1068
BstKTI GATC 2 cut(s) 85, 142
BstMAI GTCTC 3 cut(s) 211, 734, 1113
BstMBI GATC 2 cut(s) 82, 139
BstMWI GCNNNNNNNGC 4 cut(s) 1059, 1065, 1074, 1125
BstNSI RCATGY 1 cut(s) 511
BstPAI GACNNNNGTC 1 cut(s) 12
BstSFI CTRYAG 1 cut(s) 1057
BstSLI GKGCMC 1 cut(s) 1130
BstUI CGCG 1 cut(s) 1068
BstV1I GCAGC 2 cut(s) 1071, 1181
BstV2I GAAGAC 1 cut(s) 289
Bsu15I ATCGAT 1 cut(s) 491
BsuRI GGCC 1 cut(s) 1138
BsuTUI ATCGAT 1 cut(s) 491
BtgI CCRYGG 1 cut(s) 287
BtsCI GGATG 2 cut(s) 115, 172
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 5 cut(s) 509, 1047, 1051, 1064, 1070
CfoI GCGC 2 cut(s) 352, 1068
Cfr10I RCCGGY 1 cut(s) 1062
ClaI ATCGAT 1 cut(s) 491
CseI GACGC 1 cut(s) 289
DdeI CTNAG 3 cut(s) 77, 134, 1073
DpnI GATC 2 cut(s) 84, 141
DpnII GATC 2 cut(s) 82, 139
Eam1104I CTCTTC 3 cut(s) 225, 441, 1005
EarI CTCTTC 3 cut(s) 225, 441, 1005
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 1 cut(s) 287
Eco147I AGGCCT 1 cut(s) 1138
Eco24I GRGCYC 1 cut(s) 1074
Eco32I GATATC 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 1 cut(s) 1183
Eco88I CYCGRG 1 cut(s) 712
EcoICRI GAGCTC 1 cut(s) 1072
EcoRV GATATC 1 cut(s) 495
EcoT14I CCWWGG 1 cut(s) 287
EcoT22I ATGCAT 1 cut(s) 509
EcoT38I GRGCYC 1 cut(s) 1074
ErhI CCWWGG 1 cut(s) 287
Esp3I CGTCTC 1 cut(s) 1113
FalI AAGNNNNNCTT 6 cut(s) 438, 470, 1076, 1108, 1149, 1181
FblI GTMKAC 2 cut(s) 16, 606
Fnu4HI GCNGC 2 cut(s) 1060, 1195
FokI GGATG 2 cut(s) 122, 179
FriOI GRGCYC 1 cut(s) 1074
Fsp4HI GCNGC 2 cut(s) 1060, 1195
FspAI RTGCGCAY 1 cut(s) 351
FspBI CTAG 3 cut(s) 372, 1050, 1175
FspI TGCGCA 1 cut(s) 351
GlaI GCGC 2 cut(s) 351, 1067
GluI GCNGC 2 cut(s) 1060, 1195
HaeIII GGCC 1 cut(s) 1138
HapII CCGG 2 cut(s) 971, 1063
HgaI GACGC 1 cut(s) 289
HhaI GCGC 2 cut(s) 352, 1068
Hin1I GRCGYC 1 cut(s) 1106
Hin6I GCGC 2 cut(s) 350, 1066
HinP1I GCGC 2 cut(s) 350, 1066
HinfI GANTC 7 cut(s) 94, 151, 476, 618, 780, 927, 1171
HpaII CCGG 2 cut(s) 971, 1063
HphI GGTGA 1 cut(s) 637
Hpy166II GTNNAC 2 cut(s) 17, 607
Hpy8I GTNNAC 2 cut(s) 17, 607
HpyAV CCTTC 1 cut(s) 1151
HpyCH4III ACNGT 3 cut(s) 11, 70, 611
HpyCH4IV ACGT 1 cut(s) 1106
HpyCH4V TGCA 6 cut(s) 119, 176, 318, 507, 698, 1059
HpyF10VI GCNNNNNNNGC 4 cut(s) 1059, 1065, 1074, 1125
HpyF3I CTNAG 3 cut(s) 77, 134, 1073
HpySE526I ACGT 1 cut(s) 1106
Hsp92I GRCGYC 1 cut(s) 1106
HspAI GCGC 2 cut(s) 350, 1066
KroI GCCGGC 1 cut(s) 1062
KroNI GCCGGC 1 cut(s) 1064
Kzo9I GATC 2 cut(s) 82, 139
LmnI GCTCC 3 cut(s) 130, 571, 973
LpnPI CCDG 6 cut(s) 133, 216, 984, 1076, 1143, 1186
Lsp1109I GCAGC 2 cut(s) 1071, 1181
LweI GCATC 1 cut(s) 577
MaeI CTAG 3 cut(s) 372, 1050, 1175
MaeII ACGT 1 cut(s) 1106
MaeIII GTNAC 1 cut(s) 124
MalI GATC 2 cut(s) 84, 141
MboI GATC 2 cut(s) 82, 139
MboII GAAGA 8 cut(s) 242, 294, 387, 437, 458, 482, 826, 1022
MhlI GDGCHC 2 cut(s) 1074, 1130
MluCI AATT 9 cut(s) 59, 218, 357, 441, 637, 767, 835, 867, 878
Mph1103I ATGCAT 1 cut(s) 509
MroNI GCCGGC 1 cut(s) 1062
MroXI GAANNNNTTC 1 cut(s) 800
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 1077, 1152
MspCI CTTAAG 1 cut(s) 575
MspI CCGG 2 cut(s) 971, 1063
Mva1269I GAATGC 1 cut(s) 698
MvnI CGCG 1 cut(s) 1068
MwoI GCNNNNNNNGC 4 cut(s) 1059, 1065, 1074, 1125
NaeI GCCGGC 1 cut(s) 1064
NcoI CCATGG 1 cut(s) 287
NdeII GATC 2 cut(s) 82, 139
NgoMIV GCCGGC 1 cut(s) 1062
NheI GCTAGC 1 cut(s) 1049
NlaIV GGNNCC 1 cut(s) 969
NsbI TGCGCA 1 cut(s) 351
NsiI ATGCAT 1 cut(s) 509
NspI RCATGY 1 cut(s) 511
OliI CACNNNNGTG 1 cut(s) 269
PaeI GCATGC 1 cut(s) 511
PaeR7I CTCGAG 1 cut(s) 712
PceI AGGCCT 1 cut(s) 1138
PcsI WCGNNNNNNNCGW 1 cut(s) 904
PctI GAATGC 1 cut(s) 698
PdiI GCCGGC 1 cut(s) 1064
PdmI GAANNNNTTC 1 cut(s) 800
PfeI GAWTC 7 cut(s) 94, 151, 476, 618, 780, 927, 1171
PkrI GCNGC 2 cut(s) 1061, 1196
PshAI GACNNNNGTC 1 cut(s) 12
PshBI ATTAAT 1 cut(s) 870
PsiI TTATAA 1 cut(s) 225
Psp124BI GAGCTC 1 cut(s) 1074
PspN4I GGNNCC 1 cut(s) 969
PspXI VCTCGAGB 1 cut(s) 712
PstI CTGCAG 1 cut(s) 1061
PvuII CAGCTG 1 cut(s) 1152
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 1 cut(s) 1074
SatI GCNGC 2 cut(s) 1060, 1195
Sau3AI GATC 2 cut(s) 82, 139
SduI GDGCHC 2 cut(s) 1074, 1130
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 1 cut(s) 1057
Sfr274I CTCGAG 1 cut(s) 712
SlaI CTCGAG 1 cut(s) 712
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 3 cut(s) 401, 575, 712
SmoI CTYRAG 3 cut(s) 401, 575, 712
SphI GCATGC 1 cut(s) 511
Sse9I AATT 9 cut(s) 59, 218, 357, 441, 637, 767, 835, 867, 878
SseBI AGGCCT 1 cut(s) 1138
SsiI CCGC 1 cut(s) 1077
SspI AATATT 3 cut(s) 649, 829, 951
SspMI CTAG 3 cut(s) 372, 1050, 1175
SstI GAGCTC 1 cut(s) 1074
StuI AGGCCT 1 cut(s) 1138
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 3 cut(s) 11, 70, 611
TaiI ACGT 1 cut(s) 1109
TaqI TCGA 6 cut(s) 491, 713, 719, 785, 907, 1095
TasI AATT 9 cut(s) 59, 218, 357, 441, 637, 767, 835, 867, 878
TfiI GAWTC 7 cut(s) 94, 151, 476, 618, 780, 927, 1171
TscAI CASTG 1 cut(s) 75
TseI GCWGC 2 cut(s) 1059, 1194
TspDTI ATGAA 8 cut(s) 126, 183, 309, 438, 468, 472, 610, 879
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 75
Vha464I CTTAAG 1 cut(s) 575
VspI ATTAAT 1 cut(s) 870
XapI RAATTY 1 cut(s) 218
XceI RCATGY 1 cut(s) 511
XhoI CTCGAG 1 cut(s) 712
XmiI GTMKAC 2 cut(s) 16, 606
XmnI GAANNNNTTC 1 cut(s) 800
XspI CTAG 3 cut(s) 372, 1050, 1175
ZraI GACGTC 1 cut(s) 1107
Zsp2I ATGCAT 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.