Rmu_ssc0000446.1_g000021

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000446.1
OG0001259
Physical Location & Seq
Reverse (-)
69089 .. 70713
1625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000446.1_g000021.1.cds

Sequence Viewer

Length: 861 bp
atgaaagcgtcaaaggtggttattgcaaagacaacacatttatctaggtgggaatcacacaaaattaatatcaagcctggaaggaagaacatcaagaatgaccatcctgatgtgtgtgaaaaggaacatgaagaagccactttgaagattgaagagaaactcattaaggataaggaagctgattcattcatcacatcaatatccatgtcatgcatgcctaattgtgccccagatcaacccaaggacaagtccatgcccattccttttcaagtggaggagttcaagtttcaagatgcttattctaaacttgtctatggtattgaattcatgggagtcccccactccccggctaagatagacctccgtttggtcaatatattgtttgttgaggggagctgcactgagcagagggtgttgggtagcgagcataatctttggtacccgagtgatgggggtcaacgtgccagatcagggccgttgttgatgtgtctccgtgtctcggagggacgtagcctgattgtaacgccgcgtggtggtcgtcgtcaggataaggtgttgcctcaggaataccctttggcggaagtccctccgctgataggaagcggcaagcagtgtagcggggacccacttggtagtaacccagtgagtggtgctgcactcagcggagactgtcgcgcttgcaagatgaatggagaagttccgctagaggagttgagcctagcggagacccgcttgcaagatgaaaagggaggtgtcccgctagcggagttatgcctagcggagactcgcttgcaagatgaaaagggaggcgtcccgctagcggagtttctcttagcgaagactacctcacttgcaagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

286

Amino Acids

31.45

Weight (kDa)

6.35

Isoelectric Point (pI)

61.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 438
AccB7I CCANNNNNTGG 1 cut(s) 647
AccII CGCG 2 cut(s) 529, 675
AcsI RAATTY 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 810
AfaI GTAC 1 cut(s) 440
AgsI TTSAA 6 cut(s) 145, 152, 269, 283, 290, 323
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 2 cut(s) 179, 396
AluI AGCT 2 cut(s) 179, 396
Alw26I GTCTC 5 cut(s) 494, 502, 660, 719, 776
Ama87I CYCGRG 1 cut(s) 442
AoxI GGCC 1 cut(s) 473
ApeKI GCWGC 2 cut(s) 396, 653
ApoI RAATTY 1 cut(s) 323
AseI ATTAAT 1 cut(s) 66
Asp718I GGTACC 1 cut(s) 438
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 2 cut(s) 473, 622
AsuC2I CCSGG 1 cut(s) 347
AsuNHI GCTAGC 2 cut(s) 760, 817
AvaI CYCGRG 1 cut(s) 442
AvaII GGWCC 1 cut(s) 622
AxyI CCTNAGG 1 cut(s) 561
BaeGI GKGCMC 1 cut(s) 229
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BanI GGYRCC 1 cut(s) 438
BbsI GAAGAC 1 cut(s) 845
BbvI GCAGC 2 cut(s) 383, 640
BccI CCATC 2 cut(s) 111, 443
BceAI ACGGC 1 cut(s) 460
BciT130I CCWGG 1 cut(s) 78
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 5 cut(s) 494, 502, 660, 719, 776
BfaI CTAG 6 cut(s) 45, 704, 719, 761, 776, 818
BisI GCNGC 4 cut(s) 397, 527, 604, 654
BlsI GCNGC 4 cut(s) 398, 528, 605, 655
Bme1390I CCNGG 2 cut(s) 78, 347
Bme18I GGWCC 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 442
BmgT120I GGNCC 2 cut(s) 473, 622
BmiI GGNNCC 3 cut(s) 440, 623, 624
BmrFI CCNGG 2 cut(s) 78, 347
BmrI ACTGGG 1 cut(s) 635
BmsI GCATC 1 cut(s) 283
BmtI GCTAGC 2 cut(s) 764, 821
BmuI ACTGGG 1 cut(s) 635
BpiI GAAGAC 1 cut(s) 845
BpuMI CCSGG 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 810
BsaI GGTCTC 1 cut(s) 719
BsaJI CCNNGG 2 cut(s) 240, 345
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bse1I ACTGG 1 cut(s) 641
Bse21I CCTNAGG 1 cut(s) 561
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 240, 345
BseGI GGATG 1 cut(s) 103
BseMII CTCAG 3 cut(s) 393, 575, 673
BseNI ACTGG 1 cut(s) 641
BseRI GAGGAG 2 cut(s) 290, 722
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 2 cut(s) 383, 640
BsgI GTGCAG 2 cut(s) 382, 639
Bsh1236I CGCG 2 cut(s) 529, 675
BshFI GGCC 1 cut(s) 475
BshNI GGYRCC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 442
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 6 cut(s) 320, 519, 569, 635, 740, 797
BsmAI GTCTC 5 cut(s) 494, 502, 660, 719, 776
BsmFI GGGAC 6 cut(s) 320, 519, 569, 635, 740, 797
BsnI GGCC 1 cut(s) 475
Bso31I GGTCTC 1 cut(s) 719
BsoBI CYCGRG 1 cut(s) 442
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 2 cut(s) 232, 467
BspANI GGCC 1 cut(s) 475
BspCNI CTCAG 3 cut(s) 394, 574, 672
BspFNI CGCG 2 cut(s) 529, 675
BspLI GGNNCC 3 cut(s) 440, 623, 624
BspOI GCTAGC 2 cut(s) 764, 821
BspT107I GGYRCC 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 719
BsrI ACTGG 1 cut(s) 641
BssECI CCNNGG 2 cut(s) 240, 345
BssMI GATC 2 cut(s) 232, 467
BssNI GRCGYC 1 cut(s) 810
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 671
Bst6I CTCTTC 1 cut(s) 147
BstACI GRCGYC 1 cut(s) 810
BstC8I GCNNGC 8 cut(s) 215, 425, 608, 679, 734, 762, 791, 819
BstDEI CTNAG 5 cut(s) 351, 402, 561, 659, 832
BstF5I GGATG 1 cut(s) 103
BstFNI CGCG 2 cut(s) 529, 675
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 2 cut(s) 235, 470
BstMAI GTCTC 5 cut(s) 494, 502, 660, 719, 776
BstMBI GATC 2 cut(s) 232, 467
BstNI CCWGG 1 cut(s) 78
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 76, 345
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 2 cut(s) 529, 675
BstV1I GCAGC 2 cut(s) 383, 640
BstV2I GAAGAC 1 cut(s) 845
Bsu36I CCTNAGG 1 cut(s) 561
BsuRI GGCC 1 cut(s) 475
BtsCI GGATG 1 cut(s) 103
BtsI GCAGTG 1 cut(s) 617
BtsIMutI CAGTG 3 cut(s) 399, 617, 648
Cac8I GCNNGC 8 cut(s) 215, 425, 608, 679, 734, 762, 791, 819
CfoI GCGC 1 cut(s) 677
Cfr13I GGNCC 2 cut(s) 473, 622
CseI GACGC 1 cut(s) 799
Csp6I GTAC 1 cut(s) 439
CviAII CATG 6 cut(s) 128, 205, 210, 214, 253, 328
CviJI RGCY 8 cut(s) 76, 137, 179, 350, 396, 475, 513, 717
CviKI_1 RGCY 8 cut(s) 76, 137, 179, 350, 396, 475, 513, 717
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 5 cut(s) 351, 402, 561, 659, 832
DpnI GATC 2 cut(s) 234, 469
DpnII GATC 2 cut(s) 232, 467
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
EciI GGCGGA 1 cut(s) 593
Eco130I CCWWGG 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 719
Eco47I GGWCC 1 cut(s) 622
Eco81I CCTNAGG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 442
EcoO109I RGGNCCY 1 cut(s) 622
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 240
EcoT22I ATGCAT 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 6 cut(s) 131, 208, 213, 217, 256, 331
FaqI GGGAC 6 cut(s) 320, 519, 569, 635, 740, 797
FatI CATG 6 cut(s) 127, 204, 209, 213, 252, 327
FauI CCCGC 4 cut(s) 611, 737, 765, 822
Fnu4HI GCNGC 4 cut(s) 397, 527, 604, 654
FokI GGATG 1 cut(s) 90
Fsp4HI GCNGC 4 cut(s) 397, 527, 604, 654
FspBI CTAG 6 cut(s) 45, 704, 719, 761, 776, 818
GlaI GCGC 1 cut(s) 676
GluI GCNGC 4 cut(s) 397, 527, 604, 654
HaeIII GGCC 1 cut(s) 475
HapII CCGG 1 cut(s) 347
HgaI GACGC 1 cut(s) 799
HhaI GCGC 1 cut(s) 677
Hin1I GRCGYC 1 cut(s) 810
Hin1II CATG 6 cut(s) 131, 208, 213, 217, 256, 331
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HincII GTYRAC 1 cut(s) 458
HindII GTYRAC 1 cut(s) 458
HinfI GANTC 4 cut(s) 53, 182, 333, 784
HpaII CCGG 1 cut(s) 347
Hpy166II GTNNAC 1 cut(s) 458
Hpy188I TCNGA 1 cut(s) 502
Hpy188III TCNNGA 5 cut(s) 94, 107, 290, 545, 563
Hpy8I GTNNAC 1 cut(s) 458
Hpy99I CGWCG 1 cut(s) 543
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 1 cut(s) 671
HpyCH4IV ACGT 2 cut(s) 460, 508
HpyCH4V TGCA 8 cut(s) 26, 213, 399, 656, 681, 736, 793, 854
HpyF3I CTNAG 5 cut(s) 351, 402, 561, 659, 832
HpySE526I ACGT 2 cut(s) 460, 508
Hsp92I GRCGYC 1 cut(s) 810
Hsp92II CATG 6 cut(s) 131, 208, 213, 217, 256, 331
HspAI GCGC 1 cut(s) 675
KflI GGGWCCC 1 cut(s) 622
KpnI GGTACC 1 cut(s) 442
Kzo9I GATC 2 cut(s) 232, 467
LmnI GCTCC 1 cut(s) 393
Lsp1109I GCAGC 2 cut(s) 383, 640
LweI GCATC 1 cut(s) 283
MaeI CTAG 6 cut(s) 45, 704, 719, 761, 776, 818
MaeII ACGT 2 cut(s) 460, 508
MaeIII GTNAC 2 cut(s) 520, 635
MalI GATC 2 cut(s) 234, 469
MboI GATC 2 cut(s) 232, 467
MboII GAAGA 5 cut(s) 97, 143, 157, 164, 850
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 3 cut(s) 63, 220, 323
MlyI GAGTC 2 cut(s) 342, 778
Mph1103I ATGCAT 1 cut(s) 215
MseI TTAA 2 cut(s) 66, 165
MslI CAYNNNNRTG 1 cut(s) 108
MspA1I CMGCKG 2 cut(s) 592, 663
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 2 cut(s) 78, 347
MvaI CCWGG 1 cut(s) 78
MvnI CGCG 2 cut(s) 529, 675
NciI CCSGG 1 cut(s) 347
NdeII GATC 2 cut(s) 232, 467
NheI GCTAGC 2 cut(s) 760, 817
NlaIII CATG 6 cut(s) 131, 208, 213, 217, 256, 331
NlaIV GGNNCC 3 cut(s) 440, 623, 624
NsiI ATGCAT 1 cut(s) 215
NspI RCATGY 1 cut(s) 217
PaeI GCATGC 1 cut(s) 217
PfeI GAWTC 2 cut(s) 53, 182
PflMI CCANNNNNTGG 1 cut(s) 647
PkrI GCNGC 4 cut(s) 398, 528, 605, 655
PleI GAGTC 2 cut(s) 341, 778
PpsI GAGTC 2 cut(s) 341, 778
PpuMI RGGWCCY 1 cut(s) 622
PshBI ATTAAT 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 3 cut(s) 440, 623, 624
PspPI GGNCC 2 cut(s) 473, 622
PspPPI RGGWCCY 1 cut(s) 622
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
RseI CAYNNNNRTG 1 cut(s) 108
SaqAI TTAA 2 cut(s) 66, 165
SatI GCNGC 4 cut(s) 397, 527, 604, 654
Sau3AI GATC 2 cut(s) 232, 467
Sau96I GGNCC 2 cut(s) 473, 622
SchI GAGTC 2 cut(s) 342, 778
ScrFI CCNGG 2 cut(s) 78, 347
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 1 cut(s) 283
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 108
SphI GCATGC 1 cut(s) 217
Sse9I AATT 3 cut(s) 63, 220, 323
SspMI CTAG 6 cut(s) 45, 704, 719, 761, 776, 818
StyD4I CCNGG 2 cut(s) 76, 345
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 671
TaiI ACGT 2 cut(s) 463, 511
TasI AATT 3 cut(s) 63, 220, 323
TauI GCSGC 2 cut(s) 529, 606
TfiI GAWTC 2 cut(s) 53, 182
Tru1I TTAA 2 cut(s) 66, 165
Tru9I TTAA 2 cut(s) 66, 165
TscAI CASTG 3 cut(s) 406, 617, 648
TseI GCWGC 2 cut(s) 396, 653
TspDTI ATGAA 8 cut(s) 17, 144, 174, 178, 316, 701, 756, 813
TspGWI ACGGA 2 cut(s) 353, 482
TspRI CASTG 3 cut(s) 406, 617, 648
Van91I CCANNNNNTGG 1 cut(s) 647
VpaK11BI GGWCC 1 cut(s) 622
VspI ATTAAT 1 cut(s) 66
XapI RAATTY 1 cut(s) 323
XceI RCATGY 1 cut(s) 217
XspI CTAG 6 cut(s) 45, 704, 719, 761, 776, 818
Zsp2I ATGCAT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.