RchiOBHm_Chr6g0290461

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001259
Physical Location & Seq
Reverse (-)
53466171 .. 53469396
3226 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26060

Sequence Viewer

Length: 2241 bp
ATGGTTTTCAGACCCGAGTGCAAACCAATTCAGACTCGGTTGCATTTTCCAATTTGCATGATGGATGCTGTAGTTCCACATCAAGTTGAGCATGACTTTTCAGATGTATTTGGTCCTGTACCAGTTCAGACCCTAGTGGATTCAGCTAATTCTGCCTCTCTCCAAGATAGTACGGATCCCGTCGTCATTCACAACCGGTCACACTCTTTGATTGGTCCTTCATTCTATGTAAGCGATTCACTGAACCTCAACAAACTGACTATTTATAACACAGAAGATTCAGTGGACCTAGCAGAGTACTGTCTCCATGAAGAAACCATCAAAGAATCTCAGGAACCTTTTGTTGGTGACCCTGTCATTGGGCATCCTCTAGAAAATAACTTTGTAGCAAACAAAGTTGTGGATTATTTTCAATTACCGACAGTGAAGCTGAGTGATCCATACTGGATTCCATTTGGAGAGGATGAATATGCACAAGTAACAAAGGTTTGTAAAGTTCCTGTCTCTATGGGAAAATTTTATAAAGAAGAGATTACTTGTCATGTGATCGACATGGACGACACTGATGTGCTTTTTGGAATACCATGGCATGAAAGCGTCAAAGGTGGTTTTTGCAAAGACAACACATATTTATTTCAGTGGGAGTCACACAAAATTAAAATCAAGCTTGAAAGGATGAACATCAAGAATGACCATCCTGAGGTGTGTGAAAAGGAACATGAAGAAGGCACTTTGAAGATTGAAGAGAAACTCATTAAGGACACGGAAGCTGATTCATTCATCACACCGATATCCATGTCATGCATGCCTAATTGTGTTCAAGATCAACCCAAGGAGAAGTCAATGCCCATTCCTTTTCAAGTGGAGGAGTTCAAATTTCAAGATGTTTATTCTAAACTTGTCTACGGTATTGGATTCATGGTGATACCTTTTAATTTCGAAAATATTGAAGTTGATGGCTTATCATGTTTTATTCCTACTAACGATCAAGCTGCATACAAGAGGAACTCGAGGTCGAGTTCTTTTCAGGTGGAGGAGACTGATGTAGGACGAGATTTTAGCCAACTTCAACAAAGAATCTCGAAAAGAAAGAAGCGTCTGCACATTAAGAAGAATGATTTGAATATTGAAAATTGGTATTCAAATAGGCTTCTTAATGATGGAATTAATCATAAATTACTCTTTTGGATATACCGGGATTCTCGAGCGAAATCTTGGTTGAGATTCCAAATATATGTTTGCGCACCTTGTGAGAGTGTAGGCATCGAAGATTTTGAGGTTTTGAAGATTGTTGGGCAGGGTGGATTTGCAAAAGTATATCAGGTGAGGAAGAAGGGAACCTCGGAAATATACGCGATGAAGGTAATGCGAAAGGACAAGATCATGAAGAATAATCTTGCTGAATACATGAAATTTGAGAGGGATATACTAACAAAAGTTGATCATCCATTCATTGTCAAGCTTAGATACTCATTCCAAACCAAATACAGACTATATCTTGTGCTGGATTTTGTCAACGGAGGTCAGCTTTTCTTTCAACTCTACCACCATGGCCTTTTTTTGGAGGAGCTGGCACGAATATATGCAGCCGAGATTGTTTCTGCGGTGTCTCACCTTCATTCAAATGGCATAATGCATAGAGATGTTAAGCCTGAAAATATCCTATTGGATGCAGATGGCCATGCCATTTTAACTGATTTTGGGCTTGCGAAGAGCTTTGACGAAAACACGAGGTCTAATTCTCTATGTGGAACTGTAGAATACATGGCACCGGAAATAGTTCAGGGAAATGGCCATGATAAGGCAGCAGATTGGTGGAGTGTGGGAATTGTATTGTTTCAGATGCTTACTGGCAAGCTTCCTTTTACTGGGAACCGTCAGAAATGTCAAGAGAAGATAGTAAAGGAGAAAATTAAGTTGCCAAGTTTTTTGTCAAGTGAAGCACATTCTCTGTTGAAAGGCTTGTTAGCGAAGGACACAAGCAAGCGCCTAGGTTGTGGACCTCTGGGAGGTGATGAAGTCAAGCAGCACAAATGGTTCAAGCAAATTAATTGGAAGAAATTGGATGCTCGTGAAATCAAACCGAGCTTCCGACCTAACGTTCCCGGGCAGCACTGCATTGCCAACATTGAGAATTACTGGACTGACATGCCACTTGTGGACTCCCCGGCTGCTAGTCCGAAGGCTTCGTCTGGAAATCCCTTCTCCGGCTTTAGTTATGTTAGGTCTGCAGCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008285 GO:0009266 GO:0009408 GO:0009409 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0035556 GO:0036211 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045727 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04350 ko04371 ko04666 ko04714 ko04910 ko04931 ko05165 ko05200 ko05205 ko05210 ko05212 ko05221 ko05224 ko05225 ko05226 ko05231 map01521 map01522 map04012 map04066 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04350 map04371 map04666 map04714 map04910 map04931 map05165 map05200 map05205 map05210 map05212 map05221 map05224 map05225 map05226 map05231
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

746

Amino Acids

85.4

Weight (kDa)

6.29

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 425 - 680 7.5e-74 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 427 - 663 1.7e-32 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 499 - 580 7.4e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 521 - 663 3.6e-09 Kinase-like
Pkinase_fungal PF17667 532 - 612 3.1e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 267, 522
Acc16I TGCGCA 1 cut(s) 1243
AccB1I GGYRCC 1 cut(s) 1768
AccI GTMKAC 1 cut(s) 903
AccII CGCG 1 cut(s) 1355
AciI CCGC 1 cut(s) 1604
AclI AACGTT 1 cut(s) 2100
AclWI GGATC 3 cut(s) 170, 183, 431
AcoI YGGCCR 2 cut(s) 1678, 1792
AcsI RAATTY 3 cut(s) 515, 875, 1412
AdeI CACNNNGTG 1 cut(s) 1250
AfaI GTAC 3 cut(s) 120, 172, 299
AfiI CCNNNNNNNGG 8 cut(s) 344, 359, 700, 1561, 1801, 1868, 2009, 2207
AgeI ACCGGT 1 cut(s) 195
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AleI CACNNNNGTG 1 cut(s) 566
Alw26I GTCTC 4 cut(s) 308, 508, 1031, 1614
AlwI GGATC 3 cut(s) 170, 183, 431
Ama87I CYCGRG 4 cut(s) 14, 1009, 1203, 2105
AoxI GGCC 3 cut(s) 1552, 1678, 1792
ApeKI GCWGC 7 cut(s) 992, 1586, 1805, 2026, 2110, 2171, 2231
ApoI RAATTY 3 cut(s) 515, 875, 1412
ArsI GACNNNNNNTTYG 2 cut(s) 2174, 2206
AseI ATTAAT 2 cut(s) 1167, 2049
AsiGI ACCGGT 1 cut(s) 195
Asp700I GAANNNNTTC 1 cut(s) 1779
AspA2I CCTAGG 1 cut(s) 1990
AspLEI GCGC 2 cut(s) 1244, 1989
AspS9I GGNCC 4 cut(s) 113, 215, 286, 2000
AsuC2I CCSGG 4 cut(s) 1196, 2106, 2107, 2168
AsuHPI GGTGA 5 cut(s) 359, 934, 1336, 1604, 2024
AsuII TTCGAA 1 cut(s) 939
AvaI CYCGRG 4 cut(s) 14, 1009, 1203, 2105
AvaII GGWCC 4 cut(s) 113, 215, 286, 2000
AvrII CCTAGG 1 cut(s) 1990
AxyI CCTNAGG 1 cut(s) 699
BalI TGGCCA 2 cut(s) 1680, 1794
BamHI GGATCC 1 cut(s) 175
BanI GGYRCC 1 cut(s) 1768
BauI CACGAG 2 cut(s) 1729, 2070
BbvI GCAGC 6 cut(s) 979, 1598, 1817, 2038, 2122, 2158
BccI CCATC 6 cut(s) 55, 326, 702, 950, 1154, 1670
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BclI TGATCA 1 cut(s) 1441
BcnI CCSGG 4 cut(s) 1196, 2106, 2107, 2168
BcoDI GTCTC 4 cut(s) 308, 508, 1031, 1614
BfaI CTAG 5 cut(s) 134, 290, 371, 1991, 2175
BfmI CTRYAG 3 cut(s) 69, 1755, 2229
BfoI RGCGCY 1 cut(s) 1990
BisI GCNGC 7 cut(s) 993, 1587, 1806, 2027, 2111, 2172, 2232
BlnI CCTAGG 1 cut(s) 1990
BlsI GCNGC 7 cut(s) 994, 1588, 1807, 2028, 2112, 2173, 2233
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 4 cut(s) 1196, 2106, 2107, 2168
Bme18I GGWCC 4 cut(s) 113, 215, 286, 2000
BmeT110I CYCGRG 4 cut(s) 14, 1009, 1203, 2105
BmgT120I GGNCC 4 cut(s) 113, 215, 286, 2000
BmiI GGNNCC 5 cut(s) 177, 336, 1339, 1770, 1874
BmrFI CCNGG 4 cut(s) 1196, 2106, 2107, 2168
BmrI ACTGGG 1 cut(s) 1878
BmsI GCATC 6 cut(s) 55, 373, 1272, 1660, 1833, 2056
BmuI ACTGGG 1 cut(s) 1878
Bpu14I TTCGAA 1 cut(s) 939
BpuMI CCSGG 4 cut(s) 1196, 2106, 2107, 2168
BsaBI GATNNNNATC 1 cut(s) 680
BsaJI CCNNGG 7 cut(s) 584, 831, 1341, 1549, 1990, 2105, 2166
BsaWI WCCGGW 2 cut(s) 195, 1771
Bsc4I CCNNNNNNNGG 8 cut(s) 344, 359, 700, 1561, 1801, 1868, 2009, 2207
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 5 cut(s) 122, 449, 1855, 1873, 2144
Bse21I CCTNAGG 1 cut(s) 699
Bse3DI GCAATG 1 cut(s) 2118
Bse8I GATNNNNATC 1 cut(s) 680
BseDI CCNNGG 7 cut(s) 584, 831, 1341, 1549, 1990, 2105, 2166
BseGI GGATG 8 cut(s) 70, 364, 469, 681, 694, 1444, 1675, 2071
BseJI GATNNNNATC 1 cut(s) 680
BseLI CCNNNNNNNGG 8 cut(s) 344, 359, 700, 1561, 1801, 1868, 2009, 2207
BseMI GCAATG 1 cut(s) 2118
BseMII CTCAG 3 cut(s) 344, 422, 690
BseNI ACTGG 5 cut(s) 122, 449, 1855, 1873, 2144
BseRI GAGGAG 3 cut(s) 881, 1049, 1580
BseXI GCAGC 6 cut(s) 979, 1598, 1817, 2038, 2122, 2158
BsgI GTGCAG 1 cut(s) 1085
Bsh1236I CGCG 1 cut(s) 1355
BshFI GGCC 3 cut(s) 1554, 1680, 1794
BshNI GGYRCC 1 cut(s) 1768
BshTI ACCGGT 1 cut(s) 195
BsiHKCI CYCGRG 4 cut(s) 14, 1009, 1203, 2105
BsiSI CCGG 6 cut(s) 196, 1195, 1772, 2106, 2168, 2208
BslI CCNNNNNNNGG 8 cut(s) 344, 359, 700, 1561, 1801, 1868, 2009, 2207
BsmAI GTCTC 4 cut(s) 308, 508, 1031, 1614
BsnI GGCC 3 cut(s) 1554, 1680, 1794
BsoBI CYCGRG 4 cut(s) 14, 1009, 1203, 2105
Bsp119I TTCGAA 1 cut(s) 939
Bsp143I GATC 7 cut(s) 175, 436, 546, 823, 985, 1380, 1441
Bsp19I CCATGG 2 cut(s) 584, 1549
BspACI CCGC 1 cut(s) 1604
BspANI GGCC 3 cut(s) 1554, 1680, 1794
BspCNI CTCAG 3 cut(s) 343, 423, 691
BspFNI CGCG 1 cut(s) 1355
BspHI TCATGA 1 cut(s) 1383
BspLI GGNNCC 5 cut(s) 177, 336, 1339, 1770, 1874
BspMAI CTGCAG 1 cut(s) 2233
BspPI GGATC 3 cut(s) 170, 183, 431
BspQI GCTCTTC 1 cut(s) 1706
BspT104I TTCGAA 1 cut(s) 939
BspT107I GGYRCC 1 cut(s) 1768
BsrDI GCAATG 1 cut(s) 2118
BsrFI RCCGGY 1 cut(s) 195
BsrI ACTGG 5 cut(s) 122, 449, 1855, 1873, 2144
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 7 cut(s) 584, 831, 1341, 1549, 1990, 2105, 2166
BssMI GATC 7 cut(s) 175, 436, 546, 823, 985, 1380, 1441
BssSI CACGAG 2 cut(s) 1729, 2070
BssT1I CCWWGG 4 cut(s) 584, 831, 1549, 1990
Bst2BI CACGAG 2 cut(s) 1729, 2070
Bst4CI ACNGT 5 cut(s) 302, 424, 908, 1756, 1877
Bst6I CTCTTC 3 cut(s) 522, 738, 1706
BstBI TTCGAA 1 cut(s) 939
BstC8I GCNNGC 5 cut(s) 806, 1572, 1707, 1856, 1985
BstDEI CTNAG 4 cut(s) 330, 431, 699, 1463
BstDSI CCRYGG 2 cut(s) 584, 1549
BstEII GGTNACC 1 cut(s) 347
BstENI CCTNNNNNAGG 1 cut(s) 2007
BstF5I GGATG 8 cut(s) 70, 364, 469, 681, 694, 1444, 1675, 2071
BstFNI CGCG 1 cut(s) 1355
BstH2I RGCGCY 1 cut(s) 1990
BstHHI GCGC 2 cut(s) 1244, 1989
BstKTI GATC 7 cut(s) 178, 439, 549, 826, 988, 1383, 1444
BstMAI GTCTC 4 cut(s) 308, 508, 1031, 1614
BstMBI GATC 7 cut(s) 175, 436, 546, 823, 985, 1380, 1441
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNSI RCATGY 2 cut(s) 808, 2152
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 4 cut(s) 1194, 2104, 2105, 2166
BstSFI CTRYAG 3 cut(s) 69, 1755, 2229
BstUI CGCG 1 cut(s) 1355
BstV1I GCAGC 6 cut(s) 979, 1598, 1817, 2038, 2122, 2158
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
Bsu36I CCTNAGG 1 cut(s) 699
BsuRI GGCC 3 cut(s) 1554, 1680, 1794
BtgI CCRYGG 2 cut(s) 584, 1549
BtgZI GCGATG 1 cut(s) 1370
BtsCI GGATG 8 cut(s) 70, 364, 469, 681, 694, 1444, 1675, 2071
BtsI GCAGTG 1 cut(s) 2113
BtsIMutI CAGTG 6 cut(s) 239, 288, 429, 561, 644, 2113
Cac8I GCNNGC 5 cut(s) 806, 1572, 1707, 1856, 1985
CciI TCATGA 1 cut(s) 1383
CfoI GCGC 2 cut(s) 1244, 1989
Cfr10I RCCGGY 1 cut(s) 195
Cfr13I GGNCC 4 cut(s) 113, 215, 286, 2000
Cfr9I CCCGGG 1 cut(s) 2105
CseI GACGC 2 cut(s) 586, 1085
Csp6I GTAC 3 cut(s) 119, 171, 298
CspAI ACCGGT 1 cut(s) 195
CviQI GTAC 3 cut(s) 119, 171, 298
DdeI CTNAG 4 cut(s) 330, 431, 699, 1463
DpnI GATC 7 cut(s) 177, 438, 548, 825, 987, 1382, 1443
DpnII GATC 7 cut(s) 175, 436, 546, 823, 985, 1380, 1441
DraIII CACNNNGTG 1 cut(s) 1250
EaeI YGGCCR 2 cut(s) 1678, 1792
Eam1104I CTCTTC 3 cut(s) 522, 738, 1706
EarI CTCTTC 3 cut(s) 522, 738, 1706
Eco130I CCWWGG 4 cut(s) 584, 831, 1549, 1990
Eco32I GATATC 1 cut(s) 792
Eco47I GGWCC 4 cut(s) 113, 215, 286, 2000
Eco81I CCTNAGG 1 cut(s) 699
Eco88I CYCGRG 4 cut(s) 14, 1009, 1203, 2105
Eco91I GGTNACC 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 2007
EcoO65I GGTNACC 1 cut(s) 347
EcoRV GATATC 1 cut(s) 792
EcoT14I CCWWGG 4 cut(s) 584, 831, 1549, 1990
EcoT22I ATGCAT 2 cut(s) 806, 1638
ErhI CCWWGG 4 cut(s) 584, 831, 1549, 1990
FbaI TGATCA 1 cut(s) 1441
FblI GTMKAC 1 cut(s) 903
Fnu4HI GCNGC 7 cut(s) 993, 1587, 1806, 2027, 2111, 2172, 2232
FokI GGATG 8 cut(s) 77, 351, 476, 681, 688, 1431, 1682, 2078
Fsp4HI GCNGC 7 cut(s) 993, 1587, 1806, 2027, 2111, 2172, 2232
FspBI CTAG 5 cut(s) 134, 290, 371, 1991, 2175
FspI TGCGCA 1 cut(s) 1243
GlaI GCGC 2 cut(s) 1243, 1988
GluI GCNGC 7 cut(s) 993, 1587, 1806, 2027, 2111, 2172, 2232
HaeII RGCGCY 1 cut(s) 1990
HaeIII GGCC 3 cut(s) 1554, 1680, 1794
HapII CCGG 6 cut(s) 196, 1195, 1772, 2106, 2168, 2208
HgaI GACGC 2 cut(s) 586, 1085
HhaI GCGC 2 cut(s) 1244, 1989
Hin6I GCGC 2 cut(s) 1242, 1987
HinP1I GCGC 2 cut(s) 1242, 1987
HincII GTYRAC 1 cut(s) 1516
HindII GTYRAC 1 cut(s) 1516
HindIII AAGCTT 3 cut(s) 665, 1460, 1856
HpaII CCGG 6 cut(s) 196, 1195, 1772, 2106, 2168, 2208
HphI GGTGA 5 cut(s) 359, 934, 1336, 1604, 2024
Hpy166II GTNNAC 5 cut(s) 286, 904, 1516, 2000, 2161
Hpy188I TCNGA 9 cut(s) 11, 33, 103, 129, 1345, 1842, 1881, 2093, 2181
Hpy8I GTNNAC 5 cut(s) 286, 904, 1516, 2000, 2161
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 8 cut(s) 228, 719, 1327, 1354, 1625, 1966, 2176, 2212
HpyCH4III ACNGT 5 cut(s) 302, 424, 908, 1756, 1877
HpyCH4IV ACGT 1 cut(s) 2100
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 4 cut(s) 330, 431, 699, 1463
HpySE526I ACGT 1 cut(s) 2100
HspAI GCGC 2 cut(s) 1242, 1987
Ksp22I TGATCA 1 cut(s) 1441
Kzo9I GATC 7 cut(s) 175, 436, 546, 823, 985, 1380, 1441
LguI GCTCTTC 1 cut(s) 1706
LmnI GCTCC 1 cut(s) 1567
Lsp1109I GCAGC 6 cut(s) 979, 1598, 1817, 2038, 2122, 2158
LweI GCATC 6 cut(s) 55, 373, 1272, 1660, 1833, 2056
MaeI CTAG 5 cut(s) 134, 290, 371, 1991, 2175
MaeII ACGT 1 cut(s) 2100
MaeIII GTNAC 4 cut(s) 198, 347, 478, 645
MalI GATC 7 cut(s) 177, 438, 548, 825, 987, 1382, 1443
MboI GATC 7 cut(s) 175, 436, 546, 823, 985, 1380, 1441
MflI RGATCY 1 cut(s) 175
MlsI TGGCCA 2 cut(s) 1680, 1794
MluNI TGGCCA 2 cut(s) 1680, 1794
MlyI GAGTC 3 cut(s) 28, 653, 2156
MmeI TCCRAC 1 cut(s) 2116
Mox20I TGGCCA 2 cut(s) 1680, 1794
Mph1103I ATGCAT 2 cut(s) 806, 1638
MroXI GAANNNNTTC 1 cut(s) 1779
MscI TGGCCA 2 cut(s) 1680, 1794
MslI CAYNNNNRTG 3 cut(s) 566, 1623, 1641
Msp20I TGGCCA 2 cut(s) 1680, 1794
MspI CCGG 6 cut(s) 196, 1195, 1772, 2106, 2168, 2208
MspR9I CCNGG 4 cut(s) 1196, 2106, 2107, 2168
MvnI CGCG 1 cut(s) 1355
MwoI GCNNNNNNNGC 1 cut(s) 152
NciI CCSGG 4 cut(s) 1196, 2106, 2107, 2168
NcoI CCATGG 2 cut(s) 584, 1549
NdeII GATC 7 cut(s) 175, 436, 546, 823, 985, 1380, 1441
NlaIV GGNNCC 5 cut(s) 177, 336, 1339, 1770, 1874
NmeAIII GCCGAG 1 cut(s) 1615
NmuCI GTSAC 3 cut(s) 198, 347, 645
NsbI TGCGCA 1 cut(s) 1243
NsiI ATGCAT 2 cut(s) 806, 1638
NspI RCATGY 2 cut(s) 808, 2152
NspV TTCGAA 1 cut(s) 939
OliI CACNNNNGTG 1 cut(s) 566
PaeI GCATGC 1 cut(s) 808
PaeR7I CTCGAG 2 cut(s) 1009, 1203
PagI TCATGA 1 cut(s) 1383
PciSI GCTCTTC 1 cut(s) 1706
PcsI WCGNNNNNNNCGW 1 cut(s) 555
PdmI GAANNNNTTC 1 cut(s) 1779
PflFI GACNNNGTC 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 195
PkrI GCNGC 7 cut(s) 994, 1588, 1807, 2028, 2112, 2173, 2233
PleI GAGTC 3 cut(s) 28, 652, 2156
PpsI GAGTC 3 cut(s) 28, 652, 2156
PshBI ATTAAT 2 cut(s) 1167, 2049
PsiI TTATAA 2 cut(s) 267, 522
Psp1406I AACGTT 1 cut(s) 2100
PspEI GGTNACC 1 cut(s) 347
PspN4I GGNNCC 5 cut(s) 177, 336, 1339, 1770, 1874
PspPI GGNCC 4 cut(s) 113, 215, 286, 2000
PspXI VCTCGAGB 1 cut(s) 1009
PstI CTGCAG 1 cut(s) 2233
PsuI RGATCY 1 cut(s) 175
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 3 cut(s) 120, 172, 299
RsaNI GTAC 3 cut(s) 119, 171, 298
RseI CAYNNNNRTG 3 cut(s) 566, 1623, 1641
SapI GCTCTTC 1 cut(s) 1706
SatI GCNGC 7 cut(s) 993, 1587, 1806, 2027, 2111, 2172, 2232
Sau3AI GATC 7 cut(s) 175, 436, 546, 823, 985, 1380, 1441
Sau96I GGNCC 4 cut(s) 113, 215, 286, 2000
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 3 cut(s) 28, 653, 2156
ScrFI CCNGG 4 cut(s) 1196, 2106, 2107, 2168
SfaNI GCATC 6 cut(s) 55, 373, 1272, 1660, 1833, 2056
SfcI CTRYAG 3 cut(s) 69, 1755, 2229
Sfr274I CTCGAG 2 cut(s) 1009, 1203
SfuI TTCGAA 1 cut(s) 939
SinI GGWCC 4 cut(s) 113, 215, 286, 2000
SlaI CTCGAG 2 cut(s) 1009, 1203
SmaI CCCGGG 1 cut(s) 2107
SmiMI CAYNNNNRTG 3 cut(s) 566, 1623, 1641
SmlI CTYRAG 2 cut(s) 1009, 1203
SmoI CTYRAG 2 cut(s) 1009, 1203
SphI GCATGC 1 cut(s) 808
SsiI CCGC 1 cut(s) 1604
SspI AATATT 2 cut(s) 946, 1126
SspMI CTAG 5 cut(s) 134, 290, 371, 1991, 2175
StyD4I CCNGG 4 cut(s) 1194, 2104, 2105, 2166
StyI CCWWGG 4 cut(s) 584, 831, 1549, 1990
TaaI ACNGT 5 cut(s) 302, 424, 908, 1756, 1877
TaiI ACGT 1 cut(s) 2103
TaqI TCGA 7 cut(s) 549, 939, 1010, 1016, 1082, 1204, 1266
TatI WGTACW 1 cut(s) 297
TscAI CASTG 6 cut(s) 246, 288, 429, 568, 644, 2120
TseFI GTSAC 3 cut(s) 198, 347, 645
TseI GCWGC 7 cut(s) 992, 1586, 1805, 2026, 2110, 2171, 2231
Tsp45I GTSAC 3 cut(s) 198, 347, 645
TspGWI ACGGA 3 cut(s) 188, 779, 1533
TspMI CCCGGG 1 cut(s) 2105
TspRI CASTG 6 cut(s) 246, 288, 429, 568, 644, 2120
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 4 cut(s) 113, 215, 286, 2000
VspI ATTAAT 2 cut(s) 1167, 2049
XagI CCTNNNNNAGG 1 cut(s) 2007
XapI RAATTY 3 cut(s) 515, 875, 1412
XbaI TCTAGA 1 cut(s) 370
XceI RCATGY 2 cut(s) 808, 2152
XhoI CTCGAG 2 cut(s) 1009, 1203
XmaI CCCGGG 1 cut(s) 2105
XmaJI CCTAGG 1 cut(s) 1990
XmiI GTMKAC 1 cut(s) 903
XmnI GAANNNNTTC 1 cut(s) 1779
XspI CTAG 5 cut(s) 134, 290, 371, 1991, 2175
ZrmI AGTACT 1 cut(s) 299
Zsp2I ATGCAT 2 cut(s) 806, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.