Rmu_sc0008421.1_g000002

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008421.1
OG0001259
Physical Location & Seq
Reverse (-)
16840 .. 17223
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008421.1_g000002.1.cds

Sequence Viewer

Length: 384 bp
atggacgacactaatgtgctttttggaagatcatggcatgaaagcgtcaaaggtggttattgcaaagacaacacatatttactaaggtgggagtcgcacaaaattaaaatcaagcttagaaggaagaacatcaaaaatgaccatcctgaggtgtgtgacaaggaacatgaagaagcaactttgaagattgaagagaaactcattaaggataaggaagctgattcattcatcacatcgatatccatgtcatgcatgcctaattgtgttcaagatcaacccaaggagaagtcattgcccgttccttttcaagtggaggagttcaagtttcaagatacttattccaaactttgtctacggtattggattcatggtgataccttttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

15.09

Weight (kDa)

6.51

Isoelectric Point (pI)

57.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 352
AfiI CCNNNNNNNGG 1 cut(s) 148
AgsI TTSAA 6 cut(s) 184, 191, 269, 308, 322, 329
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 2 cut(s) 115, 218
AluI AGCT 2 cut(s) 115, 218
AsuHPI GGTGA 1 cut(s) 383
AxyI CCTNAGG 1 cut(s) 147
BccI CCATC 1 cut(s) 150
Bsa29I ATCGAT 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 279
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse21I CCTNAGG 1 cut(s) 147
Bse3DI GCAATG 1 cut(s) 290
BseCI ATCGAT 1 cut(s) 236
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 1 cut(s) 138
BseRI GAGGAG 1 cut(s) 329
BshVI ATCGAT 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 148
Bsp143I GATC 2 cut(s) 29, 271
BspCNI CTCAG 1 cut(s) 139
BspDI ATCGAT 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 2 cut(s) 29, 271
BssT1I CCWWGG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 357
Bst6I CTCTTC 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 3 cut(s) 83, 116, 147
BstF5I GGATG 1 cut(s) 142
BstKTI GATC 2 cut(s) 32, 274
BstMBI GATC 2 cut(s) 29, 271
BstNSI RCATGY 1 cut(s) 256
Bsu15I ATCGAT 1 cut(s) 236
Bsu36I CCTNAGG 1 cut(s) 147
BsuTUI ATCGAT 1 cut(s) 236
BtsCI GGATG 1 cut(s) 142
Cac8I GCNNGC 1 cut(s) 254
ClaI ATCGAT 1 cut(s) 236
CseI GACGC 1 cut(s) 34
CviAII CATG 7 cut(s) 33, 38, 167, 244, 249, 253, 368
CviJI RGCY 2 cut(s) 115, 218
CviKI_1 RGCY 2 cut(s) 115, 218
DdeI CTNAG 3 cut(s) 83, 116, 147
DpnI GATC 2 cut(s) 31, 273
DpnII GATC 2 cut(s) 29, 271
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 279
Eco32I GATATC 1 cut(s) 240
Eco81I CCTNAGG 1 cut(s) 147
EcoRV GATATC 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 279
EcoT22I ATGCAT 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 7 cut(s) 36, 41, 170, 247, 252, 256, 371
FaiI YATR 8 cut(s) 34, 39, 76, 168, 245, 250, 254, 369
FatI CATG 7 cut(s) 32, 37, 166, 243, 248, 252, 367
FblI GTMKAC 1 cut(s) 352
FokI GGATG 1 cut(s) 129
HgaI GACGC 1 cut(s) 34
Hin1II CATG 7 cut(s) 36, 41, 170, 247, 252, 256, 371
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 3 cut(s) 92, 221, 364
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 353
Hpy188III TCNNGA 3 cut(s) 146, 269, 329
Hpy8I GTNNAC 1 cut(s) 353
HpyAV CCTTC 1 cut(s) 114
HpyCH4III ACNGT 1 cut(s) 357
HpyCH4V TGCA 2 cut(s) 63, 252
HpyF3I CTNAG 3 cut(s) 83, 116, 147
Hsp92II CATG 7 cut(s) 36, 41, 170, 247, 252, 256, 371
Kzo9I GATC 2 cut(s) 29, 271
LpnPI CCDG 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 155
MalI GATC 2 cut(s) 31, 273
MboI GATC 2 cut(s) 29, 271
MboII GAAGA 5 cut(s) 39, 136, 182, 196, 203
MluCI AATT 2 cut(s) 102, 259
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 2 cut(s) 142, 307
Mph1103I ATGCAT 1 cut(s) 254
MseI TTAA 3 cut(s) 105, 204, 382
MslI CAYNNNNRTG 1 cut(s) 14
NdeII GATC 2 cut(s) 29, 271
NlaIII CATG 7 cut(s) 36, 41, 170, 247, 252, 256, 371
NmuCI GTSAC 1 cut(s) 155
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 1 cut(s) 256
OliI CACNNNNGTG 1 cut(s) 14
PaeI GCATGC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 221, 364
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 14
SaqAI TTAA 3 cut(s) 105, 204, 382
Sau3AI GATC 2 cut(s) 29, 271
SchI GAGTC 1 cut(s) 101
SetI ASST 6 cut(s) 55, 89, 117, 153, 220, 380
SmiMI CAYNNNNRTG 1 cut(s) 14
SphI GCATGC 1 cut(s) 256
Sse9I AATT 2 cut(s) 102, 259
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 1 cut(s) 357
TaqI TCGA 1 cut(s) 236
TasI AATT 2 cut(s) 102, 259
TfiI GAWTC 2 cut(s) 221, 364
Tru1I TTAA 3 cut(s) 105, 204, 382
Tru9I TTAA 3 cut(s) 105, 204, 382
TseFI GTSAC 1 cut(s) 155
Tsp45I GTSAC 1 cut(s) 155
TspDTI ATGAA 5 cut(s) 54, 183, 213, 217, 356
XceI RCATGY 1 cut(s) 256
XmiI GTMKAC 1 cut(s) 352
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.