Rmu_sc0000423.1_g000017

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000423.1
OG0001259
Physical Location & Seq
Reverse (-)
56503 .. 58373
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000423.1_g000017.1.cds

Sequence Viewer

Length: 1164 bp
atggatgacactaatgtgctttttggaagaccatggaatgaaagcgtcaaaggtggttattgcaaagacaacacatatttatttaggtgggagtcgcacaaaattaaaatcaagcctggaaggaagaacatcaagaatgatcatcctgaggtgtgtgaaaaggaacatgaagaagccactttgaagattgaagagaaactcattaaggacaaggaagctgattcattcatcacatcgatatccatgtcatgcatgcctaattgtgttcaagatcaacctaaggagaagtcaatgcccattccttttcaagtggaagagttcaagtttcaagatgcttattctaaacttgtctacggtattggattcatggtggtaccttttaatttcgagaatattgatgttgatggcttatcatgttttattcctactaatgatcgagttgcatacaagaggaactcgaggtcgagtttttttcaagtggagaagactgatgtaggacgagattttagccattttcaacaaaaaatctcgaaaagaaagaagcgtcttcacattaagaagaataatttgaatattggaaattgggagtctcaactaggtttagcactagcccctgagccccatcggtactcccaggtactacaccatgggccaggttcacgccccaccatggcgggcctcacatgggatgccaccctgggcacccagggcccggggcaaggccagcacagcccatctatttacaccacaggagggctgatatggcatcagaagaacctgtgtcccacatcgaggataggggagactcccttcccatgcttgagtataaagacattagcacaaccaccttttacggacggtatagcattctgtgtgaagtaccctgagaaagccaccagaaacattggcgggccagataggggtgtcctggatatgaatgagccggaggtgacaggagaggagagaagtgtcgcccaggcactgatattctctccgggaacttcgtcaacagaggcgccagctgttcaaggggaaatccatggtctcggatccttggacccgatagtcccggatcaggtgactccggtatcggaattggagtccatgcgagctgagatcgcagccttcagggagcagtccaggatcgatcaagagtaccgataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

387

Amino Acids

43.52

Weight (kDa)

6.44

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1064
Acc65I GGTACC 1 cut(s) 373
AccB1I GGYRCC 3 cut(s) 373, 701, 1015
AccI GTMKAC 1 cut(s) 351
AciI CCGC 2 cut(s) 674, 909
AclWI GGATC 4 cut(s) 1044, 1057, 1080, 1151
AcuI CTGAAG 1 cut(s) 1111
AcyI GRCGYC 1 cut(s) 1016
AfaI GTAC 5 cut(s) 375, 629, 639, 881, 1157
AfiI CCNNNNNNNGG 8 cut(s) 670, 685, 712, 719, 753, 792, 920, 1075
AjnI CCWGG 8 cut(s) 115, 633, 652, 696, 705, 927, 975, 1139
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 3 cut(s) 218, 1022, 1112
AluI AGCT 3 cut(s) 218, 1022, 1112
Alw26I GTCTC 3 cut(s) 594, 797, 1049
AlwI GGATC 4 cut(s) 1044, 1057, 1080, 1151
AlwNI CAGNNNCTG 1 cut(s) 982
Ama87I CYCGRG 2 cut(s) 457, 712
AoxI GGCC 5 cut(s) 650, 676, 709, 721, 911
ApaI GGGCCC 1 cut(s) 713
ApeKI GCWGC 1 cut(s) 1121
Asp718I GGTACC 1 cut(s) 373
AspLEI GCGC 1 cut(s) 1018
AspS9I GGNCC 6 cut(s) 650, 676, 709, 710, 911, 1057
AsuC2I CCSGG 4 cut(s) 713, 714, 996, 1070
AsuHPI GGTGA 2 cut(s) 961, 1090
AvaI CYCGRG 2 cut(s) 457, 712
AvaII GGWCC 1 cut(s) 1057
AxyI CCTNAGG 2 cut(s) 147, 279
BaeGI GKGCMC 2 cut(s) 704, 713
BaeI ACNNNNGTAYC 2 cut(s) 1071, 1104
BamHI GGATCC 1 cut(s) 1049
BanI GGYRCC 3 cut(s) 373, 701, 1015
BanII GRGCYC 2 cut(s) 621, 713
BbsI GAAGAC 3 cut(s) 34, 491, 539
BbvI GCAGC 1 cut(s) 1133
BccI CCATC 3 cut(s) 398, 630, 742
BciT130I CCWGG 8 cut(s) 117, 635, 654, 698, 707, 929, 977, 1141
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 4 cut(s) 713, 714, 996, 1070
BcoDI GTCTC 3 cut(s) 594, 797, 1049
BfaI CTAG 2 cut(s) 596, 608
BfoI RGCGCY 1 cut(s) 1019
BisI GCNGC 1 cut(s) 1122
BlsI GCNGC 1 cut(s) 1123
Bme18I GGWCC 1 cut(s) 1057
BmeT110I CYCGRG 2 cut(s) 457, 712
BmgT120I GGNCC 6 cut(s) 650, 676, 709, 710, 911, 1057
BmiI GGNNCC 6 cut(s) 375, 703, 711, 1017, 1051, 1059
BmsI GCATC 3 cut(s) 322, 679, 775
BpiI GAAGAC 3 cut(s) 34, 491, 539
Bpu10I CCTNAGC 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 841
BpuMI CCSGG 4 cut(s) 713, 714, 996, 1070
Bsa29I ATCGAT 2 cut(s) 236, 1146
BsaHI GRCGYC 1 cut(s) 1016
BsaI GGTCTC 1 cut(s) 1049
BsaWI WCCGGW 1 cut(s) 1084
Bsc4I CCNNNNNNNGG 8 cut(s) 670, 685, 712, 719, 753, 792, 920, 1075
Bse21I CCTNAGG 2 cut(s) 147, 279
BseBI CCWGG 8 cut(s) 117, 635, 654, 698, 707, 929, 977, 1141
BseCI ATCGAT 2 cut(s) 236, 1146
BseGI GGATG 3 cut(s) 10, 142, 694
BseLI CCNNNNNNNGG 8 cut(s) 670, 685, 712, 719, 753, 792, 920, 1075
BseMII CTCAG 4 cut(s) 138, 606, 876, 1104
BseRI GAGGAG 1 cut(s) 974
BseSI GKGCMC 2 cut(s) 704, 713
BseXI GCAGC 1 cut(s) 1133
BshFI GGCC 5 cut(s) 652, 678, 711, 723, 913
BshNI GGYRCC 3 cut(s) 373, 701, 1015
BshVI ATCGAT 2 cut(s) 236, 1146
BsiHKCI CYCGRG 2 cut(s) 457, 712
BsiSI CCGG 5 cut(s) 713, 944, 995, 1070, 1085
BslFI GGGAC 2 cut(s) 768, 1052
BslI CCNNNNNNNGG 8 cut(s) 670, 685, 712, 719, 753, 792, 920, 1075
BsmAI GTCTC 3 cut(s) 594, 797, 1049
BsmFI GGGAC 2 cut(s) 768, 1052
BsmI GAATGC 1 cut(s) 866
BsnI GGCC 5 cut(s) 652, 678, 711, 723, 913
Bso31I GGTCTC 1 cut(s) 1049
BsoBI CYCGRG 2 cut(s) 457, 712
Bsp120I GGGCCC 1 cut(s) 709
Bsp1286I GDGCHC 3 cut(s) 621, 704, 713
Bsp143I GATC 8 cut(s) 139, 271, 433, 1049, 1072, 1116, 1143, 1147
Bsp19I CCATGG 4 cut(s) 32, 646, 669, 1039
BspACI CCGC 2 cut(s) 674, 909
BspANI GGCC 5 cut(s) 652, 678, 711, 723, 913
BspCNI CTCAG 4 cut(s) 139, 607, 877, 1105
BspDI ATCGAT 2 cut(s) 236, 1146
BspLI GGNNCC 6 cut(s) 375, 703, 711, 1017, 1051, 1059
BspPI GGATC 4 cut(s) 1044, 1057, 1080, 1151
BspT107I GGYRCC 3 cut(s) 373, 701, 1015
BspTNI GGTCTC 1 cut(s) 1049
BssMI GATC 8 cut(s) 139, 271, 433, 1049, 1072, 1116, 1143, 1147
BssNI GRCGYC 1 cut(s) 1016
BssT1I CCWWGG 5 cut(s) 32, 646, 669, 1039, 1053
Bst2UI CCWGG 8 cut(s) 117, 635, 654, 698, 707, 929, 977, 1141
Bst4CI ACNGT 2 cut(s) 356, 860
Bst6I CTCTTC 2 cut(s) 186, 309
BstACI GRCGYC 1 cut(s) 1016
BstC8I GCNNGC 6 cut(s) 254, 676, 725, 911, 1020, 1110
BstDEI CTNAG 5 cut(s) 147, 279, 615, 885, 1113
BstDSI CCRYGG 4 cut(s) 32, 646, 669, 1039
BstF5I GGATG 3 cut(s) 10, 142, 694
BstH2I RGCGCY 1 cut(s) 1019
BstHHI GCGC 1 cut(s) 1018
BstKTI GATC 8 cut(s) 142, 274, 436, 1052, 1075, 1119, 1146, 1150
BstMAI GTCTC 3 cut(s) 594, 797, 1049
BstMBI GATC 8 cut(s) 139, 271, 433, 1049, 1072, 1116, 1143, 1147
BstMWI GCNNNNNNNGC 5 cut(s) 708, 724, 729, 763, 1118
BstNI CCWGG 8 cut(s) 117, 635, 654, 698, 707, 929, 977, 1141
BstNSI RCATGY 1 cut(s) 256
BstSLI GKGCMC 2 cut(s) 704, 713
BstV1I GCAGC 1 cut(s) 1133
BstV2I GAAGAC 3 cut(s) 34, 491, 539
BstX2I RGATCY 1 cut(s) 1049
BstYI RGATCY 1 cut(s) 1049
Bsu15I ATCGAT 2 cut(s) 236, 1146
Bsu36I CCTNAGG 2 cut(s) 147, 279
BsuRI GGCC 5 cut(s) 652, 678, 711, 723, 913
BsuTUI ATCGAT 2 cut(s) 236, 1146
BtgI CCRYGG 4 cut(s) 32, 646, 669, 1039
BtsCI GGATG 3 cut(s) 10, 142, 694
BtsIMutI CAGTG 1 cut(s) 980
Cac8I GCNNGC 6 cut(s) 254, 676, 725, 911, 1020, 1110
CaiI CAGNNNCTG 1 cut(s) 982
CfoI GCGC 1 cut(s) 1018
Cfr13I GGNCC 6 cut(s) 650, 676, 709, 710, 911, 1057
Cfr9I CCCGGG 1 cut(s) 712
ClaI ATCGAT 2 cut(s) 236, 1146
CseI GACGC 2 cut(s) 34, 533
Csp6I GTAC 5 cut(s) 374, 628, 638, 880, 1156
CviQI GTAC 5 cut(s) 374, 628, 638, 880, 1156
DdeI CTNAG 5 cut(s) 147, 279, 615, 885, 1113
DinI GGCGCC 1 cut(s) 1017
DpnI GATC 8 cut(s) 141, 273, 435, 1051, 1074, 1118, 1145, 1149
DpnII GATC 8 cut(s) 139, 271, 433, 1049, 1072, 1116, 1143, 1147
DrdI GACNNNNNNGTC 1 cut(s) 1064
DseDI GACNNNNNNGTC 1 cut(s) 1064
Eam1104I CTCTTC 2 cut(s) 186, 309
EarI CTCTTC 2 cut(s) 186, 309
Eco130I CCWWGG 5 cut(s) 32, 646, 669, 1039, 1053
Eco24I GRGCYC 2 cut(s) 621, 713
Eco31I GGTCTC 1 cut(s) 1049
Eco32I GATATC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 1057
Eco57I CTGAAG 1 cut(s) 1111
Eco81I CCTNAGG 2 cut(s) 147, 279
Eco88I CYCGRG 2 cut(s) 457, 712
EcoO109I RGGNCCY 1 cut(s) 709
EcoRII CCWGG 8 cut(s) 115, 633, 652, 696, 705, 927, 975, 1139
EcoRV GATATC 1 cut(s) 240
EcoT14I CCWWGG 5 cut(s) 32, 646, 669, 1039, 1053
EcoT22I ATGCAT 1 cut(s) 254
EcoT38I GRGCYC 2 cut(s) 621, 713
EgeI GGCGCC 1 cut(s) 1017
EheI GGCGCC 1 cut(s) 1017
ErhI CCWWGG 5 cut(s) 32, 646, 669, 1039, 1053
FaqI GGGAC 2 cut(s) 768, 1052
FauI CCCGC 2 cut(s) 667, 902
FbaI TGATCA 1 cut(s) 139
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 1 cut(s) 1122
FokI GGATG 3 cut(s) 17, 129, 701
FriOI GRGCYC 2 cut(s) 621, 713
Fsp4HI GCNGC 1 cut(s) 1122
FspBI CTAG 2 cut(s) 596, 608
GlaI GCGC 1 cut(s) 1017
GluI GCNGC 1 cut(s) 1122
HaeII RGCGCY 1 cut(s) 1019
HaeIII GGCC 5 cut(s) 652, 678, 711, 723, 913
HapII CCGG 5 cut(s) 713, 944, 995, 1070, 1085
HgaI GACGC 2 cut(s) 34, 533
HhaI GCGC 1 cut(s) 1018
Hin1I GRCGYC 1 cut(s) 1016
Hin6I GCGC 1 cut(s) 1016
HinP1I GCGC 1 cut(s) 1016
HincII GTYRAC 1 cut(s) 1008
HindII GTYRAC 1 cut(s) 1008
HinfI GANTC 7 cut(s) 92, 221, 363, 587, 805, 1081, 1100
HpaII CCGG 5 cut(s) 713, 944, 995, 1070, 1085
HphI GGTGA 2 cut(s) 961, 1090
Hpy166II GTNNAC 3 cut(s) 352, 659, 1008
Hpy188I TCNGA 3 cut(s) 771, 1049, 1093
Hpy188III TCNNGA 7 cut(s) 133, 146, 269, 329, 388, 529, 1151
Hpy8I GTNNAC 3 cut(s) 352, 659, 1008
HpyAV CCTTC 3 cut(s) 114, 820, 1135
HpyCH4III ACNGT 2 cut(s) 356, 860
HpyCH4V TGCA 3 cut(s) 63, 252, 443
HpyF10VI GCNNNNNNNGC 5 cut(s) 708, 724, 729, 763, 1118
HpyF3I CTNAG 5 cut(s) 147, 279, 615, 885, 1113
Hsp92I GRCGYC 1 cut(s) 1016
HspAI GCGC 1 cut(s) 1016
KasI GGCGCC 1 cut(s) 1015
KpnI GGTACC 1 cut(s) 377
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 8 cut(s) 139, 271, 433, 1049, 1072, 1116, 1143, 1147
LmnI GCTCC 1 cut(s) 1132
Lsp1109I GCAGC 1 cut(s) 1133
LweI GCATC 3 cut(s) 322, 679, 775
MaeI CTAG 2 cut(s) 596, 608
MaeIII GTNAC 2 cut(s) 949, 1078
MalI GATC 8 cut(s) 141, 273, 435, 1051, 1074, 1118, 1145, 1149
MboI GATC 8 cut(s) 139, 271, 433, 1049, 1072, 1116, 1143, 1147
MflI RGATCY 1 cut(s) 1049
MhlI GDGCHC 3 cut(s) 621, 704, 713
MluCI AATT 6 cut(s) 102, 259, 382, 565, 580, 1094
Mly113I GGCGCC 1 cut(s) 1016
MlyI GAGTC 5 cut(s) 101, 596, 799, 1075, 1109
MnlI CCTC 9 cut(s) 142, 444, 453, 689, 746, 786, 940, 952, 1006
Mph1103I ATGCAT 1 cut(s) 254
MseI TTAA 4 cut(s) 105, 204, 381, 555
MslI CAYNNNNRTG 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 1022
MspI CCGG 5 cut(s) 713, 944, 995, 1070, 1085
Mva1269I GAATGC 1 cut(s) 866
MvaI CCWGG 8 cut(s) 117, 635, 654, 698, 707, 929, 977, 1141
MwoI GCNNNNNNNGC 5 cut(s) 708, 724, 729, 763, 1118
NarI GGCGCC 1 cut(s) 1016
NciI CCSGG 4 cut(s) 713, 714, 996, 1070
NcoI CCATGG 4 cut(s) 32, 646, 669, 1039
NdeII GATC 8 cut(s) 139, 271, 433, 1049, 1072, 1116, 1143, 1147
NlaIV GGNNCC 6 cut(s) 375, 703, 711, 1017, 1051, 1059
NmuCI GTSAC 2 cut(s) 949, 1078
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 1 cut(s) 256
OliI CACNNNNGTG 1 cut(s) 14
PaeI GCATGC 1 cut(s) 256
PaeR7I CTCGAG 1 cut(s) 457
PasI CCCWGGG 2 cut(s) 697, 706
PctI GAATGC 1 cut(s) 866
PfeI GAWTC 2 cut(s) 221, 363
PfoI TCCNGGA 4 cut(s) 927, 994, 1068, 1139
PkrI GCNGC 1 cut(s) 1123
PleI GAGTC 5 cut(s) 100, 595, 799, 1075, 1108
PluTI GGCGCC 1 cut(s) 1019
PpsI GAGTC 5 cut(s) 100, 595, 799, 1075, 1108
Psp6I CCWGG 8 cut(s) 115, 633, 652, 696, 705, 927, 975, 1139
PspGI CCWGG 8 cut(s) 115, 633, 652, 696, 705, 927, 975, 1139
PspN4I GGNNCC 6 cut(s) 375, 703, 711, 1017, 1051, 1059
PspOMI GGGCCC 1 cut(s) 709
PspPI GGNCC 6 cut(s) 650, 676, 709, 710, 911, 1057
PspXI VCTCGAGB 1 cut(s) 457
PstNI CAGNNNCTG 1 cut(s) 982
PsuI RGATCY 1 cut(s) 1049
PvuII CAGCTG 1 cut(s) 1022
RsaI GTAC 5 cut(s) 375, 629, 639, 881, 1157
RsaNI GTAC 5 cut(s) 374, 628, 638, 880, 1156
RseI CAYNNNNRTG 1 cut(s) 14
SaqAI TTAA 4 cut(s) 105, 204, 381, 555
SatI GCNGC 1 cut(s) 1122
Sau3AI GATC 8 cut(s) 139, 271, 433, 1049, 1072, 1116, 1143, 1147
Sau96I GGNCC 6 cut(s) 650, 676, 709, 710, 911, 1057
SchI GAGTC 5 cut(s) 101, 596, 799, 1075, 1109
SduI GDGCHC 3 cut(s) 621, 704, 713
SfaNI GCATC 3 cut(s) 322, 679, 775
SfoI GGCGCC 1 cut(s) 1017
Sfr274I CTCGAG 1 cut(s) 457
SinI GGWCC 1 cut(s) 1057
SlaI CTCGAG 1 cut(s) 457
SmaI CCCGGG 1 cut(s) 714
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 2 cut(s) 457, 820
SmoI CTYRAG 2 cut(s) 457, 820
SphI GCATGC 1 cut(s) 256
Sse9I AATT 6 cut(s) 102, 259, 382, 565, 580, 1094
SsiI CCGC 2 cut(s) 674, 909
SspDI GGCGCC 1 cut(s) 1015
SspI AATATT 2 cut(s) 394, 574
SspMI CTAG 2 cut(s) 596, 608
StyI CCWWGG 5 cut(s) 32, 646, 669, 1039, 1053
TaaI ACNGT 2 cut(s) 356, 860
TaqI TCGA 8 cut(s) 236, 387, 436, 458, 464, 530, 791, 1146
TasI AATT 6 cut(s) 102, 259, 382, 565, 580, 1094
TfiI GAWTC 2 cut(s) 221, 363
Tru1I TTAA 4 cut(s) 105, 204, 381, 555
Tru9I TTAA 4 cut(s) 105, 204, 381, 555
TscAI CASTG 1 cut(s) 987
TseFI GTSAC 2 cut(s) 949, 1078
TseI GCWGC 1 cut(s) 1121
Tsp45I GTSAC 2 cut(s) 949, 1078
TspDTI ATGAA 6 cut(s) 54, 183, 213, 217, 355, 950
TspGWI ACGGA 1 cut(s) 869
TspMI CCCGGG 1 cut(s) 712
TspRI CASTG 1 cut(s) 987
VpaK11BI GGWCC 1 cut(s) 1057
XceI RCATGY 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 457
XmaI CCCGGG 1 cut(s) 712
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 2 cut(s) 596, 608
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.