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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 243 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 40 |
| AgsI | TTSAA | 5 cut(s) | 76, 161, 200, 214, 221 |
| AjnI | CCWGG | 1 cut(s) | 7 |
| AluBI | AGCT | 1 cut(s) | 110 |
| AluI | AGCT | 1 cut(s) | 110 |
| AxyI | CCTNAGG | 1 cut(s) | 39 |
| BccI | CCATC | 1 cut(s) | 42 |
| BciT130I | CCWGG | 1 cut(s) | 9 |
| Bme1390I | CCNGG | 1 cut(s) | 9 |
| BmrFI | CCNGG | 1 cut(s) | 9 |
| BpuEI | CTTGAG | 1 cut(s) | 8 |
| BsaJI | CCNNGG | 1 cut(s) | 171 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 200, 230 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 40 |
| Bse21I | CCTNAGG | 1 cut(s) | 39 |
| BseBI | CCWGG | 1 cut(s) | 9 |
| BseDI | CCNNGG | 1 cut(s) | 171 |
| BseGI | GGATG | 1 cut(s) | 34 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 40 |
| BseMII | CTCAG | 1 cut(s) | 30 |
| BseRI | GAGGAG | 2 cut(s) | 98, 221 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 40 |
| Bsp143I | GATC | 1 cut(s) | 163 |
| BspCNI | CTCAG | 1 cut(s) | 31 |
| BssECI | CCNNGG | 1 cut(s) | 171 |
| BssMI | GATC | 1 cut(s) | 163 |
| BssT1I | CCWWGG | 1 cut(s) | 171 |
| Bst2UI | CCWGG | 1 cut(s) | 9 |
| BstC8I | GCNNGC | 1 cut(s) | 146 |
| BstDEI | CTNAG | 1 cut(s) | 39 |
| BstF5I | GGATG | 1 cut(s) | 34 |
| BstKTI | GATC | 1 cut(s) | 166 |
| BstMBI | GATC | 1 cut(s) | 163 |
| BstNI | CCWGG | 1 cut(s) | 9 |
| BstNSI | RCATGY | 1 cut(s) | 148 |
| BstSCI | CCNGG | 1 cut(s) | 7 |
| Bsu36I | CCTNAGG | 1 cut(s) | 39 |
| BtsCI | GGATG | 1 cut(s) | 34 |
| Cac8I | GCNNGC | 1 cut(s) | 146 |
| CviAII | CATG | 3 cut(s) | 59, 136, 145 |
| CviJI | RGCY | 3 cut(s) | 7, 68, 110 |
| CviKI_1 | RGCY | 3 cut(s) | 7, 68, 110 |
| DdeI | CTNAG | 1 cut(s) | 39 |
| DpnI | GATC | 1 cut(s) | 165 |
| DpnII | GATC | 1 cut(s) | 163 |
| Eco130I | CCWWGG | 1 cut(s) | 171 |
| Eco32I | GATATC | 1 cut(s) | 132 |
| Eco81I | CCTNAGG | 1 cut(s) | 39 |
| EcoRII | CCWGG | 1 cut(s) | 7 |
| EcoRV | GATATC | 1 cut(s) | 132 |
| EcoT14I | CCWWGG | 1 cut(s) | 171 |
| ErhI | CCWWGG | 1 cut(s) | 171 |
| FaeI | CATG | 3 cut(s) | 62, 139, 148 |
| FaiI | YATR | 3 cut(s) | 60, 137, 146 |
| FatI | CATG | 3 cut(s) | 58, 135, 144 |
| FblI | GTMKAC | 1 cut(s) | 243 |
| FokI | GGATG | 1 cut(s) | 21 |
| Hin1II | CATG | 3 cut(s) | 62, 139, 148 |
| HinfI | GANTC | 1 cut(s) | 113 |
| Hpy166II | GTNNAC | 1 cut(s) | 244 |
| Hpy188III | TCNNGA | 4 cut(s) | 25, 38, 161, 221 |
| Hpy8I | GTNNAC | 1 cut(s) | 244 |
| HpyAV | CCTTC | 1 cut(s) | 6 |
| HpyF3I | CTNAG | 1 cut(s) | 39 |
| Hsp92II | CATG | 3 cut(s) | 62, 139, 148 |
| Kzo9I | GATC | 1 cut(s) | 163 |
| LpnPI | CCDG | 2 cut(s) | 21, 51 |
| MaeIII | GTNAC | 1 cut(s) | 138 |
| MalI | GATC | 1 cut(s) | 165 |
| MboI | GATC | 1 cut(s) | 163 |
| MboII | GAAGA | 3 cut(s) | 28, 74, 88 |
| MluCI | AATT | 1 cut(s) | 151 |
| MnlI | CCTC | 4 cut(s) | 32, 34, 76, 199 |
| MseI | TTAA | 1 cut(s) | 96 |
| MspR9I | CCNGG | 1 cut(s) | 9 |
| MvaI | CCWGG | 1 cut(s) | 9 |
| NdeII | GATC | 1 cut(s) | 163 |
| NlaIII | CATG | 3 cut(s) | 62, 139, 148 |
| NmuCI | GTSAC | 1 cut(s) | 138 |
| NspI | RCATGY | 1 cut(s) | 148 |
| PaeI | GCATGC | 1 cut(s) | 148 |
| PfeI | GAWTC | 1 cut(s) | 113 |
| Psp6I | CCWGG | 1 cut(s) | 7 |
| PspGI | CCWGG | 1 cut(s) | 7 |
| SaqAI | TTAA | 1 cut(s) | 96 |
| Sau3AI | GATC | 1 cut(s) | 163 |
| ScrFI | CCNGG | 1 cut(s) | 9 |
| SetI | ASST | 3 cut(s) | 24, 45, 112 |
| SmlI | CTYRAG | 1 cut(s) | 23 |
| SmoI | CTYRAG | 1 cut(s) | 23 |
| SphI | GCATGC | 1 cut(s) | 148 |
| Sse9I | AATT | 1 cut(s) | 151 |
| StyD4I | CCNGG | 1 cut(s) | 7 |
| StyI | CCWWGG | 1 cut(s) | 171 |
| TasI | AATT | 1 cut(s) | 151 |
| TfiI | GAWTC | 1 cut(s) | 113 |
| Tru1I | TTAA | 1 cut(s) | 96 |
| Tru9I | TTAA | 1 cut(s) | 96 |
| TseFI | GTSAC | 1 cut(s) | 138 |
| Tsp45I | GTSAC | 1 cut(s) | 138 |
| TspDTI | ATGAA | 4 cut(s) | 17, 75, 105, 109 |
| XceI | RCATGY | 1 cut(s) | 148 |
| XmiI | GTMKAC | 1 cut(s) | 243 |