Rroxscaffold_6G00402470

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001259
Physical Location & Seq
Forward (+)
24927680 .. 24928355
676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00402470.1

Sequence Viewer

Length: 408 bp
ATGCTGCTTATTGGTGGGATCAATTGCGAGTTCTCATCGAGGCAAGGAAAAGAGTGTGTTCAAACATGGAGGAAGATGAAAGATATCATTCAAGAATGCAATCTTGAGAGGCGAGACGAATCTACTAAGGTTGCGTATAAGAGCCAAAATGCATATGATAGACTCATCTTTGGCGTTGGGTTCATGATTGTGCCATCAAAATTTGCAGAGGATCAGGCTAATAATCCACAGGTTCAATTGTCTAATTTTTTCTCGGGTCTTTTGTCTACCTATTCTTGTCCTTTATTCAAGATGAACTCGAGGGCGAGTTCTTTCATAGTGGAGGAAAATGATGTAGGACGAGAATGGGCCTGGTTTGTAAGACTTGATGTGGACTTATCAATATTCATTAGGAACTACAACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

135

Amino Acids

15.66

Weight (kDa)

6.29

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 266
AclWI GGATC 2 cut(s) 26, 219
AcsI RAATTY 1 cut(s) 200
AgsI TTSAA 4 cut(s) 62, 92, 236, 289
AjnI CCWGG 1 cut(s) 350
Alw26I GTCTC 1 cut(s) 108
AlwI GGATC 2 cut(s) 26, 219
Ama87I CYCGRG 2 cut(s) 253, 298
AoxI GGCC 1 cut(s) 348
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 348
AvaI CYCGRG 2 cut(s) 253, 298
BccI CCATC 1 cut(s) 202
BciT130I CCWGG 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 108
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 352
BmeT110I CYCGRG 2 cut(s) 253, 298
BmgT120I GGNCC 1 cut(s) 348
BmrFI CCNGG 1 cut(s) 352
BpuEI CTTGAG 1 cut(s) 125
BseBI CCWGG 1 cut(s) 352
BshFI GGCC 1 cut(s) 350
BsiHKCI CYCGRG 2 cut(s) 253, 298
BsmAI GTCTC 1 cut(s) 108
BsmBI CGTCTC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 1 cut(s) 350
BsoBI CYCGRG 2 cut(s) 253, 298
Bsp143I GATC 2 cut(s) 18, 211
BspANI GGCC 1 cut(s) 350
BspHI TCATGA 1 cut(s) 183
BspPI GGATC 2 cut(s) 26, 219
BssMI GATC 2 cut(s) 18, 211
Bst2UI CCWGG 1 cut(s) 352
BstDEI CTNAG 1 cut(s) 126
BstKTI GATC 2 cut(s) 21, 214
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 2 cut(s) 18, 211
BstNI CCWGG 1 cut(s) 352
BstSCI CCNGG 1 cut(s) 350
BsuRI GGCC 1 cut(s) 350
CciI TCATGA 1 cut(s) 183
Cfr13I GGNCC 1 cut(s) 348
CviAII CATG 2 cut(s) 66, 184
CviJI RGCY 3 cut(s) 144, 218, 350
CviKI_1 RGCY 3 cut(s) 144, 218, 350
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 20, 213
DpnII GATC 2 cut(s) 18, 211
Eco32I GATATC 1 cut(s) 85
Eco88I CYCGRG 2 cut(s) 253, 298
EcoRII CCWGG 1 cut(s) 350
EcoRV GATATC 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 154
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 2 cut(s) 69, 187
FaiI YATR 6 cut(s) 67, 138, 154, 156, 185, 317
FatI CATG 2 cut(s) 65, 183
FauNDI CATATG 1 cut(s) 154
FblI GTMKAC 1 cut(s) 266
Fnu4HI GCNGC 1 cut(s) 5
Fsp4HI GCNGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 1 cut(s) 350
Hin1II CATG 2 cut(s) 69, 187
HinfI GANTC 2 cut(s) 119, 162
Hpy166II GTNNAC 2 cut(s) 267, 373
Hpy188III TCNNGA 4 cut(s) 92, 104, 184, 289
Hpy8I GTNNAC 2 cut(s) 267, 373
HpyCH4V TGCA 3 cut(s) 99, 152, 206
HpyF3I CTNAG 1 cut(s) 126
Hsp92II CATG 2 cut(s) 69, 187
Kzo9I GATC 2 cut(s) 18, 211
LpnPI CCDG 4 cut(s) 200, 215, 337, 364
MalI GATC 2 cut(s) 20, 213
MboI GATC 2 cut(s) 18, 211
MboII GAAGA 1 cut(s) 85
MfeI CAATTG 2 cut(s) 22, 236
MluCI AATT 4 cut(s) 22, 200, 236, 244
MlyI GAGTC 1 cut(s) 156
MnlI CCTC 6 cut(s) 33, 63, 102, 202, 294, 316
Mph1103I ATGCAT 1 cut(s) 154
MslI CAYNNNNRTG 1 cut(s) 188
MspR9I CCNGG 1 cut(s) 352
MunI CAATTG 2 cut(s) 22, 236
Mva1269I GAATGC 1 cut(s) 101
MvaI CCWGG 1 cut(s) 352
NdeI CATATG 1 cut(s) 154
NdeII GATC 2 cut(s) 18, 211
NlaIII CATG 2 cut(s) 69, 187
NsiI ATGCAT 1 cut(s) 154
PaeR7I CTCGAG 1 cut(s) 298
PagI TCATGA 1 cut(s) 183
PctI GAATGC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 119
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 350
PspPI GGNCC 1 cut(s) 348
PspXI VCTCGAGB 1 cut(s) 298
RseI CAYNNNNRTG 1 cut(s) 188
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 2 cut(s) 18, 211
Sau96I GGNCC 1 cut(s) 348
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 1 cut(s) 352
SetI ASST 3 cut(s) 132, 234, 272
Sfr274I CTCGAG 1 cut(s) 298
SlaI CTCGAG 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 2 cut(s) 104, 298
SmoI CTYRAG 2 cut(s) 104, 298
Sse9I AATT 4 cut(s) 22, 200, 236, 244
SspI AATATT 1 cut(s) 384
StyD4I CCNGG 1 cut(s) 350
TaqI TCGA 2 cut(s) 38, 299
TasI AATT 4 cut(s) 22, 200, 236, 244
TfiI GAWTC 1 cut(s) 119
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 5 cut(s) 92, 172, 304, 308, 376
XapI RAATTY 1 cut(s) 200
XhoI CTCGAG 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 266
Zsp2I ATGCAT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.