RLG00000002994
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000656
Physical Location & Seq
Reverse (-)
42775674 .. 42782888
7215 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002994

Sequence Viewer

Length: 1020 bp
ATGGCTCTAAAAGCTGCAATCGAACTTAATGTCTTCAATATCATAGCCAATTCAGGACCTGGAGCTCATCTCACATCGAAAGAAATTGTATCTCAAATCTCCACCATGAATCCAAATGCAGCAGCTGAGAATTTGAAGCGAATATTAAGAGTTCTAAGTGTGCATTCTTTGTTATCTGTATCTCAAAGGCCAAGCTTAAATGGTAAGACAGTACTAGAAGAGACTTATGGTTTAGCAAATGATACTCTTTGCCTAGTTCCAAATGAAGATGGAGTTTCCTTGGCTCCATATATTATCTTAGCTTCTGATATGACCTTTGTGAAAAGCTTGTCCATGCTCAAAGAAACAGTGCTTGAACCTGGGAGCGTTCCTTTCAACAAGACTCATGGTGTGTCCATATATGAGTACATGTTCAAGAAACCTGAGTTGAGCCAATTGTTTAATAAGGCTATGGGACAAAGTTCCATCCTTGATTTTGAAGAGGTGTTAAATGTTTACAAAGGTTTTGAAGAGGTAGAAGAGTTGATGGATGTTGCAGGAGGCAATGGAACTACAATTGCCAAAGTAGTATCTAGGTATCCACACATTCAAGGAATTAACTTCGATTTACCTAATGTTGTCGCTCAAGGAATTGCTAAATACCAAGCAGGTGTGAAACATGTTGGTGGAGATATGTTTGAGTTGATACCAAATGCACAGTCTATTATGTTGAAGCGGATACTTCATAATTGGGACGATGATCTATGTAAGAAAATATTAAAATGTTGTTGGGAAGCATTACCAGAAATCGGGAAGGTAATAGTTGTGGAATTTGCAATACCAGAAATAGTGGAGAAAACCGCAGATTTAGAGAAAATAGTGACATTAGACATCATAATGATGGCTTATCACGGCAGTAGAGAACGAACAATTGCTGAGTTTGACTGCCTATCGAAAGCTGCAGGATTCAGTGAAATGAAGATTTTTCCGATTTCACATGGTTGTTATGTTATGGAACTTCACAAAGTTAAAACATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

340

Amino Acids

37.47

Weight (kDa)

5.65

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 1 - 67 9.4e-10 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 112 - 317 3e-52 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 638
Acc36I ACCTGC 1 cut(s) 638
AciI CCGC 2 cut(s) 715, 840
AcsI RAATTY 2 cut(s) 130, 809
AfaI GTAC 2 cut(s) 213, 407
AfiI CCNNNNNNNGG 1 cut(s) 788
AflIII ACRYGT 2 cut(s) 408, 658
AgsI TTSAA 9 cut(s) 37, 136, 356, 376, 415, 479, 509, 590, 712
AjnI CCWGG 2 cut(s) 58, 358
AluBI AGCT 7 cut(s) 14, 65, 125, 195, 302, 327, 938
AluI AGCT 7 cut(s) 14, 65, 125, 195, 302, 327, 938
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 215
AlwNI CAGNNNCTG 2 cut(s) 59, 125
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 4 cut(s) 14, 119, 122, 938
ApoI RAATTY 2 cut(s) 130, 809
ArsI GACNNNNNNTTYG 4 cut(s) 15, 47, 683, 715
AspS9I GGNCC 1 cut(s) 56
AvaII GGWCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 67
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 67
BbvI GCAGC 3 cut(s) 131, 134, 925
BccI CCATC 4 cut(s) 263, 473, 520, 874
BceAI ACGGC 1 cut(s) 907
BciT130I CCWGG 2 cut(s) 60, 360
BciVI GTATCC 2 cut(s) 588, 711
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 3 cut(s) 215, 254, 573
BfmI CTRYAG 1 cut(s) 939
BfuAI ACCTGC 1 cut(s) 638
BfuI GTATCC 2 cut(s) 588, 711
BisI GCNGC 4 cut(s) 15, 120, 123, 939
BlsI GCNGC 4 cut(s) 16, 121, 124, 940
BmcAI AGTACT 1 cut(s) 213
Bme1390I CCNGG 2 cut(s) 60, 360
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 2 cut(s) 60, 360
BpiI GAAGAC 1 cut(s) 25
BplI GAGNNNNNCTC 2 cut(s) 54, 86
BpmI CTGGAG 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 609
BsaJI CCNNGG 2 cut(s) 279, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 788
Bse3DI GCAATG 1 cut(s) 550
BseBI CCWGG 2 cut(s) 60, 360
BseDI CCNNGG 2 cut(s) 279, 359
BseGI GGATG 2 cut(s) 465, 535
BseLI CCNNNNNNNGG 1 cut(s) 788
BseMI GCAATG 1 cut(s) 550
BseMII CTCAG 3 cut(s) 117, 414, 906
BseXI GCAGC 3 cut(s) 131, 134, 925
BshFI GGCC 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 67
BslFI GGGAC 2 cut(s) 468, 746
BslI CCNNNNNNNGG 1 cut(s) 788
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 2 cut(s) 468, 746
BsmI GAATGC 1 cut(s) 163
BsnI GGCC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 1 cut(s) 739
BspACI CCGC 2 cut(s) 715, 840
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 3 cut(s) 118, 415, 907
BspLI GGNNCC 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 943
BspMI ACCTGC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 550
BssECI CCNNGG 2 cut(s) 279, 359
BssMI GATC 1 cut(s) 739
BssT1I CCWWGG 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 60, 360
Bst4CI ACNGT 3 cut(s) 211, 349, 699
Bst6I CTCTTC 4 cut(s) 213, 474, 504, 513
BstDEI CTNAG 5 cut(s) 126, 155, 298, 423, 915
BstF5I GGATG 2 cut(s) 465, 535
BstKTI GATC 1 cut(s) 742
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 1 cut(s) 739
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 60, 360
BstNSI RCATGY 2 cut(s) 412, 662
BstSCI CCNGG 2 cut(s) 58, 358
BstSFI CTRYAG 1 cut(s) 939
BstV1I GCAGC 3 cut(s) 131, 134, 925
BstV2I GAAGAC 1 cut(s) 25
BsuI GTATCC 2 cut(s) 588, 711
BsuRI GGCC 1 cut(s) 190
BtsCI GGATG 2 cut(s) 465, 535
BtsIMutI CAGTG 2 cut(s) 354, 955
BveI ACCTGC 1 cut(s) 638
CaiI CAGNNNCTG 2 cut(s) 59, 125
Cfr13I GGNCC 1 cut(s) 56
Csp6I GTAC 2 cut(s) 212, 406
CviAII CATG 6 cut(s) 106, 334, 386, 409, 659, 977
CviQI GTAC 2 cut(s) 212, 406
DdeI CTNAG 5 cut(s) 126, 155, 298, 423, 915
DpnI GATC 1 cut(s) 741
DpnII GATC 1 cut(s) 739
Eam1104I CTCTTC 4 cut(s) 213, 474, 504, 513
EarI CTCTTC 4 cut(s) 213, 474, 504, 513
Ecl136II GAGCTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 2 cut(s) 58, 358
EcoT14I CCWWGG 1 cut(s) 279
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 6 cut(s) 109, 337, 389, 412, 662, 980
FaqI GGGAC 2 cut(s) 468, 746
FatI CATG 6 cut(s) 105, 333, 385, 408, 658, 976
Fnu4HI GCNGC 4 cut(s) 15, 120, 123, 939
FokI GGATG 2 cut(s) 452, 542
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 4 cut(s) 15, 120, 123, 939
FspBI CTAG 3 cut(s) 215, 254, 573
GluI GCNGC 4 cut(s) 15, 120, 123, 939
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 1 cut(s) 190
Hin1II CATG 6 cut(s) 109, 337, 389, 412, 662, 980
HindIII AAGCTT 2 cut(s) 193, 325
HinfI GANTC 3 cut(s) 109, 382, 945
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 2 cut(s) 307, 969
Hpy188III TCNNGA 3 cut(s) 54, 415, 790
Hpy8I GTNNAC 1 cut(s) 496
HpyAV CCTTC 1 cut(s) 787
HpyCH4III ACNGT 3 cut(s) 211, 349, 699
HpyCH4V TGCA 7 cut(s) 17, 119, 163, 536, 695, 815, 941
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 5 cut(s) 126, 155, 298, 423, 915
Hsp92II CATG 6 cut(s) 109, 337, 389, 412, 662, 980
Kzo9I GATC 1 cut(s) 739
LmnI GCTCC 3 cut(s) 62, 289, 363
Lsp1109I GCAGC 3 cut(s) 131, 134, 925
MaeI CTAG 3 cut(s) 215, 254, 573
MaeIII GTNAC 1 cut(s) 859
MalI GATC 1 cut(s) 741
MboI GATC 1 cut(s) 739
MboII GAAGA 7 cut(s) 25, 230, 278, 491, 521, 530, 970
MfeI CAATTG 3 cut(s) 434, 555, 909
MhlI GDGCHC 1 cut(s) 67
MlyI GAGTC 1 cut(s) 376
MnlI CCTC 3 cut(s) 475, 505, 533
MseI TTAA 8 cut(s) 27, 146, 197, 441, 488, 597, 758, 1008
MslI CAYNNNNRTG 3 cut(s) 663, 875, 878
MspA1I CMGCKG 1 cut(s) 125
MspR9I CCNGG 2 cut(s) 60, 360
MunI CAATTG 3 cut(s) 434, 555, 909
Mva1269I GAATGC 1 cut(s) 163
MvaI CCWGG 2 cut(s) 60, 360
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 1 cut(s) 739
NlaIII CATG 6 cut(s) 109, 337, 389, 412, 662, 980
NlaIV GGNNCC 1 cut(s) 285
NmuCI GTSAC 1 cut(s) 859
NspI RCATGY 2 cut(s) 412, 662
PaqCI CACCTGC 1 cut(s) 638
PciI ACATGT 2 cut(s) 408, 658
PctI GAATGC 1 cut(s) 163
PfeI GAWTC 2 cut(s) 109, 945
PkrI GCNGC 4 cut(s) 16, 121, 124, 940
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
PpuMI RGGWCCY 1 cut(s) 56
PscI ACATGT 2 cut(s) 408, 658
Psp124BI GAGCTC 1 cut(s) 67
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 58, 358
PspGI CCWGG 2 cut(s) 58, 358
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 1 cut(s) 56
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 2 cut(s) 59, 125
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 2 cut(s) 213, 407
RsaNI GTAC 2 cut(s) 212, 406
RseI CAYNNNNRTG 3 cut(s) 663, 875, 878
SacI GAGCTC 1 cut(s) 67
SaqAI TTAA 8 cut(s) 27, 146, 197, 441, 488, 597, 758, 1008
SatI GCNGC 4 cut(s) 15, 120, 123, 939
Sau3AI GATC 1 cut(s) 739
Sau96I GGNCC 1 cut(s) 56
ScaI AGTACT 1 cut(s) 213
SchI GAGTC 1 cut(s) 376
ScrFI CCNGG 2 cut(s) 60, 360
SduI GDGCHC 1 cut(s) 67
SfcI CTRYAG 1 cut(s) 939
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 3 cut(s) 663, 875, 878
SmlI CTYRAG 1 cut(s) 624
SmoI CTYRAG 1 cut(s) 624
SsiI CCGC 2 cut(s) 715, 840
SspI AATATT 2 cut(s) 144, 756
SspMI CTAG 3 cut(s) 215, 254, 573
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 2 cut(s) 58, 358
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 3 cut(s) 211, 349, 699
TaqI TCGA 4 cut(s) 21, 77, 603, 932
TatI WGTACW 2 cut(s) 211, 405
TfiI GAWTC 2 cut(s) 109, 945
Tru1I TTAA 8 cut(s) 27, 146, 197, 441, 488, 597, 758, 1008
Tru9I TTAA 8 cut(s) 27, 146, 197, 441, 488, 597, 758, 1008
TscAI CASTG 2 cut(s) 354, 955
TseFI GTSAC 1 cut(s) 859
TseI GCWGC 4 cut(s) 14, 119, 122, 938
Tsp45I GTSAC 1 cut(s) 859
TspDTI ATGAA 4 cut(s) 122, 279, 713, 971
TspRI CASTG 2 cut(s) 354, 955
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 2 cut(s) 130, 809
XceI RCATGY 2 cut(s) 412, 662
XspI CTAG 3 cut(s) 215, 254, 573
ZrmI AGTACT 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.