Rroxscaffold_3G00230880
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000656
Physical Location & Seq
Forward (+)
15833304 .. 15838291
4988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00230880.1

Sequence Viewer

Length: 1092 bp
ATGGAAAACCAGAGAATTAGCTCAAAAAACTATGTCTCTGCTTGGAATTTGGGAGGTTTAGTTGTCACTCAAATGGCTCTAAAAGCTGCAATCGAACTCAATGTCTTCAATATCATAGCCAATTCAGGACCTGGAGCTCATGTCACATCAAAAGAAATTGTATCTCAAATCTCAACCACAAATCCAAATGTAGCAGCTGAGAATTTGAAGCGAATATTAAGAGTCCTAAGTGTGCATTCTTTGTTATCTGTATCTCAAAGGCCAAGCCCAAGTGGTAAGACAGTACTAGAAGAGACTTATGGTTTAACAAAGGATACTCTTTGCCTGGTTCCAAATGAAGATGGAGTTTCCTTAGCTCCATTTATTATGTTAACTTCTGATATGACCACTGTGAAAAGCTTGTCCATGCTTAAAGAAACAGTGCTTGAACCTGGGAGCTGTCCTTTTAACAAGGCTCATGGTGTGTCCGCATATGAGTACATGTCTGAGAAACCTGAGCTGAGCCAATTGTTCAATAAGGCTATGGGCCAAAGTTCCATCCTCGATTTTGAAGAGGTGTTAAATGTTTACAGAGGTTTTGAAGAGGTAGAAGAGTTGATGGATGTTGGAGGAGGCAATGGAACTACAATTGCCAAAGTAGTCTCTAGGTATCCACACATTCATGGGATTAACTTCGATTTACCTAATGTTGTCGCTCAAGGAATTGCGAGATACCAAGCAGGTGTGAAACATGTTGGTGGAGATATGTTTGAGTTGATACCAAATGCACAGTCCATTATGTTGAAGCGGGTACTTCATAATTGGGACGATGATGTATGCAAGAAAATATTAAAACGTTGTTGGGAAGCATTACCAGAAAACGGGAAGGTAATAGTTGTGGAATTTGCAATACCAGAAATAGTGGAGAATACCACATATTTAAAGAAAATAGTGACATTAGACGTTATTATGATGGCTTGTCACGGCAGTAGAGAACGAACAACTGCTGAGTTTGACGATCTATTGAAAGCTGTAGGATTCGGTGAAATGAAGATTTTTCCGATTTCGAATGGTTATTTTGTTATAGAACTTCACAAACTTAAAACATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

363

Amino Acids

40.1

Weight (kDa)

5.9

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 21 - 104 1.9e-10 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 136 - 340 6.4e-56 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 710
Acc36I ACCTGC 1 cut(s) 710
AciI CCGC 2 cut(s) 468, 787
AclI AACGTT 1 cut(s) 835
AcsI RAATTY 3 cut(s) 46, 202, 881
AfaI GTAC 3 cut(s) 285, 479, 792
AfiI CCNNNNNNNGG 1 cut(s) 860
AflIII ACRYGT 2 cut(s) 480, 730
AgsI TTSAA 8 cut(s) 109, 208, 428, 514, 551, 581, 784, 1006
AjnI CCWGG 3 cut(s) 130, 324, 430
AluBI AGCT 9 cut(s) 21, 86, 137, 197, 356, 399, 438, 499, 1010
AluI AGCT 9 cut(s) 21, 86, 137, 197, 356, 399, 438, 499, 1010
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 3 cut(s) 40, 287, 646
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 2 cut(s) 260, 526
ApeKI GCWGC 2 cut(s) 86, 194
ApoI RAATTY 3 cut(s) 46, 202, 881
ArsI GACNNNNNNTTYG 4 cut(s) 87, 119, 755, 787
AspS9I GGNCC 2 cut(s) 128, 526
AsuHPI GGTGA 1 cut(s) 1034
AsuII TTCGAA 1 cut(s) 1046
AvaII GGWCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 97
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 73, 206
BccI CCATC 4 cut(s) 335, 545, 592, 946
BceAI ACGGC 1 cut(s) 979
BciT130I CCWGG 3 cut(s) 132, 326, 432
BciVI GTATCC 2 cut(s) 307, 660
BcoDI GTCTC 3 cut(s) 40, 287, 646
BfaI CTAG 2 cut(s) 287, 645
BfmI CTRYAG 1 cut(s) 1011
BfuAI ACCTGC 1 cut(s) 710
BfuI GTATCC 2 cut(s) 307, 660
BisI GCNGC 2 cut(s) 87, 195
BlpI GCTNAGC 1 cut(s) 500
BlsI GCNGC 2 cut(s) 88, 196
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 3 cut(s) 132, 326, 432
Bme18I GGWCC 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 128, 526
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 3 cut(s) 132, 326, 432
BpiI GAAGAC 1 cut(s) 97
BpmI CTGGAG 1 cut(s) 153
Bpu10I CCTNAGC 2 cut(s) 352, 495
Bpu1102I GCTNAGC 1 cut(s) 500
Bpu14I TTCGAA 1 cut(s) 1046
BpuEI CTTGAG 1 cut(s) 681
BsaJI CCNNGG 1 cut(s) 431
BsaXI ACNNNNNCTCC 4 cut(s) 45, 75, 126, 156
Bsc4I CCNNNNNNNGG 1 cut(s) 860
Bse3DI GCAATG 1 cut(s) 622
BseBI CCWGG 3 cut(s) 132, 326, 432
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 2 cut(s) 537, 607
BseLI CCNNNNNNNGG 1 cut(s) 860
BseMI GCAATG 1 cut(s) 622
BseMII CTCAG 5 cut(s) 189, 477, 486, 491, 978
BseRI GAGGAG 1 cut(s) 624
BseXI GCAGC 2 cut(s) 73, 206
BshFI GGCC 2 cut(s) 262, 528
BsiHKAI GWGCWC 1 cut(s) 139
BslFI GGGAC 1 cut(s) 818
BslI CCNNNNNNNGG 1 cut(s) 860
BsmAI GTCTC 3 cut(s) 40, 287, 646
BsmFI GGGAC 1 cut(s) 818
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 2 cut(s) 262, 528
Bsp119I TTCGAA 1 cut(s) 1046
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 1 cut(s) 997
Bsp1720I GCTNAGC 1 cut(s) 500
BspACI CCGC 2 cut(s) 468, 787
BspANI GGCC 2 cut(s) 262, 528
BspCNI CTCAG 5 cut(s) 190, 478, 487, 492, 979
BspLI GGNNCC 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 710
BspT104I TTCGAA 1 cut(s) 1046
BsrDI GCAATG 1 cut(s) 622
BssECI CCNNGG 1 cut(s) 431
BssMI GATC 1 cut(s) 997
Bst2UI CCWGG 3 cut(s) 132, 326, 432
Bst4CI ACNGT 4 cut(s) 283, 391, 421, 771
Bst6I CTCTTC 4 cut(s) 285, 546, 576, 585
BstBI TTCGAA 1 cut(s) 1046
BstDEI CTNAG 7 cut(s) 198, 227, 352, 486, 495, 500, 987
BstF5I GGATG 2 cut(s) 537, 607
BstKTI GATC 1 cut(s) 1000
BstMAI GTCTC 3 cut(s) 40, 287, 646
BstMBI GATC 1 cut(s) 997
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 3 cut(s) 132, 326, 432
BstNSI RCATGY 2 cut(s) 484, 734
BstSCI CCNGG 3 cut(s) 130, 324, 430
BstSFI CTRYAG 1 cut(s) 1011
BstV1I GCAGC 2 cut(s) 73, 206
BstV2I GAAGAC 1 cut(s) 97
BsuI GTATCC 2 cut(s) 307, 660
BsuRI GGCC 2 cut(s) 262, 528
BtsCI GGATG 2 cut(s) 537, 607
BtsIMutI CAGTG 2 cut(s) 387, 426
BveI ACCTGC 1 cut(s) 710
CaiI CAGNNNCTG 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 128, 526
Csp6I GTAC 3 cut(s) 284, 478, 791
CviAII CATG 6 cut(s) 140, 406, 458, 481, 662, 731
CviQI GTAC 3 cut(s) 284, 478, 791
DdeI CTNAG 7 cut(s) 198, 227, 352, 486, 495, 500, 987
DpnI GATC 1 cut(s) 999
DpnII GATC 1 cut(s) 997
DraI TTTAAA 1 cut(s) 921
Eam1104I CTCTTC 4 cut(s) 285, 546, 576, 585
EarI CTCTTC 4 cut(s) 285, 546, 576, 585
Ecl136II GAGCTC 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 130, 324, 430
EcoT38I GRGCYC 1 cut(s) 139
FaeI CATG 6 cut(s) 143, 409, 461, 484, 665, 734
FaqI GGGAC 1 cut(s) 818
FatI CATG 6 cut(s) 139, 405, 457, 480, 661, 730
FauI CCCGC 1 cut(s) 780
FauNDI CATATG 1 cut(s) 472
Fnu4HI GCNGC 2 cut(s) 87, 195
FokI GGATG 2 cut(s) 524, 614
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 2 cut(s) 87, 195
FspBI CTAG 2 cut(s) 287, 645
GluI GCNGC 2 cut(s) 87, 195
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 2 cut(s) 262, 528
Hin1II CATG 6 cut(s) 143, 409, 461, 484, 665, 734
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HindIII AAGCTT 1 cut(s) 397
HinfI GANTC 2 cut(s) 222, 1017
HpaI GTTAAC 1 cut(s) 372
HphI GGTGA 1 cut(s) 1034
Hpy166II GTNNAC 2 cut(s) 372, 568
Hpy188I TCNGA 3 cut(s) 379, 487, 1041
Hpy188III TCNNGA 1 cut(s) 126
Hpy8I GTNNAC 2 cut(s) 372, 568
HpyAV CCTTC 1 cut(s) 859
HpyCH4III ACNGT 4 cut(s) 283, 391, 421, 771
HpyCH4IV ACGT 2 cut(s) 835, 942
HpyCH4V TGCA 5 cut(s) 89, 235, 767, 819, 887
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 7 cut(s) 198, 227, 352, 486, 495, 500, 987
HpySE526I ACGT 2 cut(s) 835, 942
Hsp92II CATG 6 cut(s) 143, 409, 461, 484, 665, 734
KspAI GTTAAC 1 cut(s) 372
Kzo9I GATC 1 cut(s) 997
LmnI GCTCC 3 cut(s) 134, 361, 435
Lsp1109I GCAGC 2 cut(s) 73, 206
MaeI CTAG 2 cut(s) 287, 645
MaeII ACGT 2 cut(s) 835, 942
MaeIII GTNAC 4 cut(s) 64, 142, 931, 959
MalI GATC 1 cut(s) 999
MboI GATC 1 cut(s) 997
MboII GAAGA 7 cut(s) 97, 302, 350, 563, 593, 602, 1042
MfeI CAATTG 2 cut(s) 506, 627
MhlI GDGCHC 1 cut(s) 139
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 1 cut(s) 586
MnlI CCTC 7 cut(s) 47, 547, 551, 566, 577, 602, 605
MslI CAYNNNNRTG 3 cut(s) 71, 660, 735
MspA1I CMGCKG 1 cut(s) 197
MspR9I CCNGG 3 cut(s) 132, 326, 432
MunI CAATTG 2 cut(s) 506, 627
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 3 cut(s) 132, 326, 432
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeI CATATG 1 cut(s) 472
NdeII GATC 1 cut(s) 997
NlaIII CATG 6 cut(s) 143, 409, 461, 484, 665, 734
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 4 cut(s) 64, 142, 931, 959
NspI RCATGY 2 cut(s) 484, 734
NspV TTCGAA 1 cut(s) 1046
PaqCI CACCTGC 1 cut(s) 710
PciI ACATGT 2 cut(s) 480, 730
PctI GAATGC 1 cut(s) 235
PfeI GAWTC 1 cut(s) 1017
PkrI GCNGC 2 cut(s) 88, 196
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 128
PscI ACATGT 2 cut(s) 480, 730
Psp124BI GAGCTC 1 cut(s) 139
Psp1406I AACGTT 1 cut(s) 835
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 3 cut(s) 130, 324, 430
PspGI CCWGG 3 cut(s) 130, 324, 430
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 2 cut(s) 128, 526
PspPPI RGGWCCY 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 3 cut(s) 285, 479, 792
RsaNI GTAC 3 cut(s) 284, 478, 791
RseI CAYNNNNRTG 3 cut(s) 71, 660, 735
SacI GAGCTC 1 cut(s) 139
SatI GCNGC 2 cut(s) 87, 195
Sau3AI GATC 1 cut(s) 997
Sau96I GGNCC 2 cut(s) 128, 526
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 1 cut(s) 231
ScrFI CCNGG 3 cut(s) 132, 326, 432
SduI GDGCHC 1 cut(s) 139
SfcI CTRYAG 1 cut(s) 1011
SfuI TTCGAA 1 cut(s) 1046
SinI GGWCC 1 cut(s) 128
SmiMI CAYNNNNRTG 3 cut(s) 71, 660, 735
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
SsiI CCGC 2 cut(s) 468, 787
SspI AATATT 2 cut(s) 216, 828
SspMI CTAG 2 cut(s) 287, 645
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 3 cut(s) 130, 324, 430
TaaI ACNGT 4 cut(s) 283, 391, 421, 771
TaiI ACGT 2 cut(s) 838, 945
TaqI TCGA 4 cut(s) 93, 543, 675, 1046
TatI WGTACW 2 cut(s) 283, 477
TfiI GAWTC 1 cut(s) 1017
TscAI CASTG 2 cut(s) 394, 426
TseFI GTSAC 4 cut(s) 64, 142, 931, 959
TseI GCWGC 2 cut(s) 86, 194
Tsp45I GTSAC 4 cut(s) 64, 142, 931, 959
TspDTI ATGAA 4 cut(s) 351, 650, 785, 1043
TspRI CASTG 2 cut(s) 394, 426
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 3 cut(s) 46, 202, 881
XceI RCATGY 2 cut(s) 484, 734
XspI CTAG 2 cut(s) 287, 645
ZrmI AGTACT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.