Rroxscaffold_1G00061940
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000656
Physical Location & Seq
Reverse (-)
83959785 .. 83961188
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061940.1

Sequence Viewer

Length: 891 bp
ATGGAAAGTCATGGAAGTTTAGAAAATTATACTTCAGCAAGGGGTTTGGCAAATTACATTGCCATTCAAATGACTCTGAGAGCTGCAGTTGAGCTCAATGTGTTCAACATCATTGCCAAATCAGGTGCCGGGACTCATCTGACATCAAAAGAAATTGTTTCACAAATCCCCAACACAAATCCAACTTTAGCAGGTGAGAATTTGGAGAGAATGCTCAAACTTTTGAGTGTTCATTCTCTTTTATCCACATTTGTAAGCCCAAACCCTATTGATAAGACAGAGCAAGAAAAAAGTTATGGGCTAACAAAGGAAGCACTTTGCCTAGTCCCGAATGAAGATGGAGTTTCTTTTGCTCCGGTTGTTCTGTTTTGCTCCGAAATGCTCGTTGCGAAGAGCTTCTATATGCTCAAGTACAAGGTGCTTGAACCGGATAGTACTCCCTTTCTCAAAGCCCATGGAGAGAGTTTTTATGATTACATGTCTAACATGCCTGAGCTGTGCCAATTGTTTGACAAGATAATGGAAGCAAATTCTCACCTTTATTCTGATCATAAGGTGTTTGAGTGGGTACTCCATAATTGGGATGATGAACACTGTAAGAAGATATTGAGAAACTGTTGGGAGGCAATACCGGATTATGGGAAGGTAATAGTTGTTGAGCACAATGTAATGCCTGAAATAGTTGAGAATACCCCAGAAATGAAGCAAGTGTTATGTATGGACATATTCATGATGACCATGTTTTCCAGTGGTAAGGAGCGAACAACTGCTGAATTGGATATCCTGGCTAAATCCGTAGGTTTTGTTGAAACAAAGAACTTCCCGCTTCCTCGTGGTAGTCATGTTATTGAGTTTCTTAAGGGCGGGAGCGTGGGATCATTGGATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

296

Amino Acids

33.3

Weight (kDa)

5.4

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 16 - 109 3.5e-10 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 183 - 268 5.4e-17 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 182
Acc36I ACCTGC 1 cut(s) 182
AccB1I GGYRCC 1 cut(s) 125
AciI CCGC 2 cut(s) 824, 864
AclWI GGATC 1 cut(s) 883
AcsI RAATTY 2 cut(s) 199, 529
AcuI CTGAAG 1 cut(s) 18
AfaI GTAC 3 cut(s) 413, 436, 570
AfiI CCNNNNNNNGG 2 cut(s) 580, 638
AflII CTTAAG 1 cut(s) 857
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 4 cut(s) 68, 106, 425, 809
AjnI CCWGG 1 cut(s) 783
AluBI AGCT 4 cut(s) 83, 94, 396, 496
AluI AGCT 4 cut(s) 83, 94, 396, 496
Alw21I GWGCWC 2 cut(s) 96, 663
AlwI GGATC 1 cut(s) 883
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 2 cut(s) 199, 529
Asp700I GAANNNNTTC 1 cut(s) 395
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 2 cut(s) 206, 527
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 96
BauI CACGAG 1 cut(s) 831
Bbv12I GWGCWC 2 cut(s) 96, 663
BbvI GCAGC 1 cut(s) 70
BccI CCATC 1 cut(s) 332
BciT130I CCWGG 1 cut(s) 785
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 1 cut(s) 130
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 857
BfuAI ACCTGC 1 cut(s) 182
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 2 cut(s) 130, 785
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 2 cut(s) 130, 785
Bpu10I CCTNAGC 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 130
BsaJI CCNNGG 1 cut(s) 454
BsaWI WCCGGW 3 cut(s) 355, 427, 631
Bsc4I CCNNNNNNNGG 2 cut(s) 580, 638
Bse1I ACTGG 1 cut(s) 747
Bse3DI GCAATG 2 cut(s) 57, 111
BseBI CCWGG 1 cut(s) 785
BseDI CCNNGG 1 cut(s) 454
BseGI GGATG 1 cut(s) 589
BseLI CCNNNNNNNGG 2 cut(s) 580, 638
BseMI GCAATG 2 cut(s) 57, 111
BseMII CTCAG 2 cut(s) 68, 483
BseNI ACTGG 1 cut(s) 747
BseXI GCAGC 1 cut(s) 70
BshNI GGYRCC 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 96, 663
BsiSI CCGG 4 cut(s) 129, 356, 428, 632
BslFI GGGAC 2 cut(s) 145, 311
BslI CCNNNNNNNGG 2 cut(s) 580, 638
BsmFI GGGAC 2 cut(s) 145, 311
BsmI GAATGC 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 96, 663
Bsp143I GATC 2 cut(s) 547, 875
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 2 cut(s) 824, 864
BspCNI CTCAG 2 cut(s) 69, 484
BspHI TCATGA 1 cut(s) 729
BspLI GGNNCC 1 cut(s) 127
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 182
BspPI GGATC 1 cut(s) 883
BspQI GCTCTTC 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 125
BspTI CTTAAG 1 cut(s) 857
BsrDI GCAATG 2 cut(s) 57, 111
BsrI ACTGG 1 cut(s) 747
BssECI CCNNGG 1 cut(s) 454
BssMI GATC 2 cut(s) 547, 875
BssSI CACGAG 1 cut(s) 831
BssT1I CCWWGG 1 cut(s) 454
Bst2BI CACGAG 1 cut(s) 831
Bst2UI CCWGG 1 cut(s) 785
Bst4CI ACNGT 2 cut(s) 596, 617
Bst6I CTCTTC 1 cut(s) 386
BstAFI CTTAAG 1 cut(s) 857
BstDEI CTNAG 2 cut(s) 77, 492
BstDSI CCRYGG 1 cut(s) 454
BstF5I GGATG 1 cut(s) 589
BstKTI GATC 2 cut(s) 550, 878
BstMBI GATC 2 cut(s) 547, 875
BstNI CCWGG 1 cut(s) 785
BstNSI RCATGY 2 cut(s) 481, 490
BstSCI CCNGG 2 cut(s) 128, 783
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 70
BtgI CCRYGG 1 cut(s) 454
BtsCI GGATG 1 cut(s) 589
BtsIMutI CAGTG 2 cut(s) 592, 754
BveI ACCTGC 1 cut(s) 182
CciI TCATGA 1 cut(s) 729
Csp6I GTAC 3 cut(s) 412, 435, 569
CviAII CATG 7 cut(s) 11, 455, 478, 487, 730, 739, 842
CviJI RGCY 8 cut(s) 83, 94, 258, 301, 396, 452, 496, 788
CviKI_1 RGCY 8 cut(s) 83, 94, 258, 301, 396, 452, 496, 788
CviQI GTAC 3 cut(s) 412, 435, 569
DdeI CTNAG 2 cut(s) 77, 492
DpnI GATC 2 cut(s) 549, 877
DpnII GATC 2 cut(s) 547, 875
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 1 cut(s) 96
Eco32I GATATC 1 cut(s) 781
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 783
EcoRV GATATC 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 454
FaeI CATG 7 cut(s) 14, 458, 481, 490, 733, 742, 845
FaqI GGGAC 2 cut(s) 145, 311
FatI CATG 7 cut(s) 10, 454, 477, 486, 729, 738, 841
FauI CCCGC 2 cut(s) 831, 857
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 1 cut(s) 323
GluI GCNGC 1 cut(s) 84
HapII CCGG 4 cut(s) 129, 356, 428, 632
Hin1II CATG 7 cut(s) 14, 458, 481, 490, 733, 742, 845
HinfI GANTC 2 cut(s) 73, 133
HpaII CCGG 4 cut(s) 129, 356, 428, 632
HphI GGTGA 2 cut(s) 206, 527
Hpy188I TCNGA 4 cut(s) 78, 141, 376, 547
Hpy188III TCNNGA 2 cut(s) 328, 730
HpyAV CCTTC 1 cut(s) 637
HpyCH4III ACNGT 2 cut(s) 596, 617
HpyCH4V TGCA 1 cut(s) 86
HpyF3I CTNAG 2 cut(s) 77, 492
Hsp92II CATG 7 cut(s) 14, 458, 481, 490, 733, 742, 845
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 2 cut(s) 547, 875
LguI GCTCTTC 1 cut(s) 386
LmnI GCTCC 4 cut(s) 358, 377, 757, 867
Lsp1109I GCAGC 1 cut(s) 70
MaeI CTAG 1 cut(s) 323
MalI GATC 2 cut(s) 549, 877
MboI GATC 2 cut(s) 547, 875
MboII GAAGA 3 cut(s) 347, 403, 613
MfeI CAATTG 1 cut(s) 503
MhlI GDGCHC 2 cut(s) 96, 663
MluCI AATT 8 cut(s) 25, 52, 153, 199, 503, 529, 577, 773
MlyI GAGTC 2 cut(s) 67, 127
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 2 cut(s) 616, 840
MroXI GAANNNNTTC 1 cut(s) 395
MseI TTAA 1 cut(s) 858
MslI CAYNNNNRTG 2 cut(s) 68, 728
MspCI CTTAAG 1 cut(s) 857
MspI CCGG 4 cut(s) 129, 356, 428, 632
MspR9I CCNGG 2 cut(s) 130, 785
MunI CAATTG 1 cut(s) 503
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 1 cut(s) 785
NciI CCSGG 1 cut(s) 130
NcoI CCATGG 1 cut(s) 454
NdeII GATC 2 cut(s) 547, 875
NlaIII CATG 7 cut(s) 14, 458, 481, 490, 733, 742, 845
NlaIV GGNNCC 1 cut(s) 127
NspI RCATGY 2 cut(s) 481, 490
PagI TCATGA 1 cut(s) 729
PaqCI CACCTGC 1 cut(s) 182
PciI ACATGT 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 386
PctI GAATGC 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 395
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 2 cut(s) 67, 127
PpsI GAGTC 2 cut(s) 67, 127
PscI ACATGT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 783
PspGI CCWGG 1 cut(s) 783
PspN4I GGNNCC 1 cut(s) 127
PstI CTGCAG 1 cut(s) 88
RsaI GTAC 3 cut(s) 413, 436, 570
RsaNI GTAC 3 cut(s) 412, 435, 569
RseI CAYNNNNRTG 2 cut(s) 68, 728
SacI GAGCTC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 386
SaqAI TTAA 1 cut(s) 858
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 547, 875
ScaI AGTACT 1 cut(s) 436
SchI GAGTC 2 cut(s) 67, 127
ScrFI CCNGG 2 cut(s) 130, 785
SduI GDGCHC 2 cut(s) 96, 663
SfcI CTRYAG 1 cut(s) 84
SmiMI CAYNNNNRTG 2 cut(s) 68, 728
SmlI CTYRAG 2 cut(s) 407, 857
SmoI CTYRAG 2 cut(s) 407, 857
Sse9I AATT 8 cut(s) 25, 52, 153, 199, 503, 529, 577, 773
SsiI CCGC 2 cut(s) 824, 864
SspMI CTAG 1 cut(s) 323
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 2 cut(s) 128, 783
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 2 cut(s) 596, 617
TasI AATT 8 cut(s) 25, 52, 153, 199, 503, 529, 577, 773
TatI WGTACW 2 cut(s) 411, 434
Tru1I TTAA 1 cut(s) 858
Tru9I TTAA 1 cut(s) 858
TscAI CASTG 2 cut(s) 599, 754
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 5 cut(s) 221, 348, 603, 716, 718
TspGWI ACGGA 1 cut(s) 784
TspRI CASTG 2 cut(s) 599, 754
Vha464I CTTAAG 1 cut(s) 857
XapI RAATTY 2 cut(s) 199, 529
XceI RCATGY 2 cut(s) 481, 490
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 1 cut(s) 323
ZrmI AGTACT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.