Rh5CG359500
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000656
Physical Location & Seq
Reverse (-)
42679575 .. 42681283
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG359500.1

Sequence Viewer

Length: 804 bp
ATGGAAAATCTTGGAAGTTTAGAAAATCATGCTTCAGCTTGGGGTTTGGCAAATTTCCTCACCATTCAAATGACTCTGAGAGCTGCAGTTGAGCTCAATGTGTTCAACATCATTGCCAAATCAGGTCCCGGAGCTCATCTGACATCAGAAGAAATTGTTTCACAAATTCCCAACACAAATCCAACTTTAGCAGGTGAGAATTTGGAGCGAATTCTCAAACTTTTGAGTGTTCATTCTCTTTTATCCACATTTGTAACACCAAACCCTATTGATAAGAAGCAAGAAACAAGATATGGGCTGACAAAGGAAGCACTTTGCCTAGTCCAGAATGAAGATGGAGTTTCCTTTTCTCCAGTTGTTGTGTTTGGCTCCGAAATGTTCGTTGCACAGAGCTTCTACATGCTCAAGTACAAGGTGCTTGAACCGGAGAGTTCTCCCTTTCTCAAAGCCCATGGAGAGAGTTTTTATGACTACATGTCTAACAAGCCTGAGTTGTGCCAATTGTTTGACAAGGTGATGGGACTAAATTCTTACCTATATTTTGATCATAAGGTGTTTGAGGTTTACAAAGGTTTCGAAGAGGTGAAGGAGCTGATGGATGTAGGAGGTGGTGATGGAACTACGATTGCCAAGATAGTGTCTAGGTATCCTCACATCCATGGAATCAACTTCAATTTGCCAAGTGTTATATCTAAAGCTCCTAAGTTGCCAGGTGTAAAGCATGTAAGTGGAGATATGTTTGAGTTAATACCAGAGACGCAGTCACTCATGTTGATGGTTAGCTTGTTCATCCTTTTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00950 ko01110 map00950 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

267

Amino Acids

29.75

Weight (kDa)

5.5

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 14 - 109 2.2e-10 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 132 - 259 4.5e-24 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 182
Acc36I ACCTGC 1 cut(s) 182
AcsI RAATTY 5 cut(s) 52, 165, 199, 210, 526
AcuI CTGAAG 1 cut(s) 18
AfaI GTAC 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 4 cut(s) 68, 106, 422, 673
AhdI GACNNNNNGTC 1 cut(s) 475
AjnI CCWGG 1 cut(s) 709
AluBI AGCT 8 cut(s) 38, 83, 94, 134, 393, 592, 698, 783
AluI AGCT 8 cut(s) 38, 83, 94, 134, 393, 592, 698, 783
Alw21I GWGCWC 2 cut(s) 96, 136
Alw26I GTCTC 1 cut(s) 749
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 5 cut(s) 52, 165, 199, 210, 526
AspS9I GGNCC 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 5 cut(s) 52, 206, 526, 595, 623
AsuII TTCGAA 1 cut(s) 576
AvaII GGWCC 1 cut(s) 125
BanII GRGCYC 2 cut(s) 96, 136
Bbv12I GWGCWC 2 cut(s) 96, 136
BbvI GCAGC 1 cut(s) 70
BccI CCATC 5 cut(s) 329, 511, 589, 608, 769
BciT130I CCWGG 1 cut(s) 711
BciVI GTATCC 1 cut(s) 657
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 749
BfaI CTAG 2 cut(s) 320, 642
BfmI CTRYAG 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 182
BfuI GTATCC 1 cut(s) 657
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 129, 711
Bme18I GGWCC 1 cut(s) 125
BmeRI GACNNNNNGTC 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 127, 370
BmrFI CCNGG 2 cut(s) 129, 711
BpmI CTGGAG 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 451, 658
BsaWI WCCGGW 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 353
Bse3DI GCAATG 1 cut(s) 111
BseBI CCWGG 1 cut(s) 711
BseDI CCNNGG 2 cut(s) 451, 658
BseGI GGATG 3 cut(s) 604, 654, 789
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 68, 480
BseNI ACTGG 1 cut(s) 353
BseXI GCAGC 1 cut(s) 70
BsiHKAI GWGCWC 2 cut(s) 96, 136
BsiSI CCGG 2 cut(s) 129, 425
BslFI GGGAC 2 cut(s) 111, 534
BsmAI GTCTC 1 cut(s) 749
BsmBI CGTCTC 1 cut(s) 749
BsmFI GGGAC 2 cut(s) 111, 534
Bsp119I TTCGAA 1 cut(s) 576
Bsp1286I GDGCHC 2 cut(s) 96, 136
Bsp143I GATC 1 cut(s) 544
Bsp19I CCATGG 2 cut(s) 451, 658
BspCNI CTCAG 2 cut(s) 69, 481
BspLI GGNNCC 2 cut(s) 127, 370
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 182
BspT104I TTCGAA 1 cut(s) 576
BsrDI GCAATG 1 cut(s) 111
BsrI ACTGG 1 cut(s) 353
BssECI CCNNGG 2 cut(s) 451, 658
BssMI GATC 1 cut(s) 544
BssT1I CCWWGG 2 cut(s) 451, 658
Bst2UI CCWGG 1 cut(s) 711
Bst6I CTCTTC 1 cut(s) 573
BstBI TTCGAA 1 cut(s) 576
BstDEI CTNAG 3 cut(s) 77, 489, 702
BstDSI CCRYGG 2 cut(s) 451, 658
BstF5I GGATG 3 cut(s) 604, 654, 789
BstKTI GATC 1 cut(s) 547
BstMAI GTCTC 1 cut(s) 749
BstMBI GATC 1 cut(s) 544
BstNI CCWGG 1 cut(s) 711
BstNSI RCATGY 3 cut(s) 403, 478, 725
BstSCI CCNGG 2 cut(s) 127, 709
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 70
BsuI GTATCC 1 cut(s) 657
BtgI CCRYGG 2 cut(s) 451, 658
BtsCI GGATG 3 cut(s) 604, 654, 789
BveI ACCTGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 125
CseI GACGC 1 cut(s) 766
Csp6I GTAC 1 cut(s) 409
CviAII CATG 7 cut(s) 29, 400, 452, 475, 659, 722, 769
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 3 cut(s) 77, 489, 702
DpnI GATC 1 cut(s) 546
DpnII GATC 1 cut(s) 544
DriI GACNNNNNGTC 1 cut(s) 475
Eam1104I CTCTTC 1 cut(s) 573
Eam1105I GACNNNNNGTC 1 cut(s) 475
EarI CTCTTC 1 cut(s) 573
Ecl136II GAGCTC 2 cut(s) 94, 134
Eco130I CCWWGG 2 cut(s) 451, 658
Eco24I GRGCYC 2 cut(s) 96, 136
Eco47I GGWCC 1 cut(s) 125
Eco53kI GAGCTC 2 cut(s) 94, 134
Eco57I CTGAAG 1 cut(s) 18
EcoICRI GAGCTC 2 cut(s) 94, 134
EcoO109I RGGNCCY 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 709
EcoT14I CCWWGG 2 cut(s) 451, 658
EcoT38I GRGCYC 2 cut(s) 96, 136
ErhI CCWWGG 2 cut(s) 451, 658
Esp3I CGTCTC 1 cut(s) 749
FaeI CATG 7 cut(s) 32, 403, 455, 478, 662, 725, 772
FaqI GGGAC 2 cut(s) 111, 534
FatI CATG 7 cut(s) 28, 399, 451, 474, 658, 721, 768
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 3 cut(s) 611, 641, 776
FriOI GRGCYC 2 cut(s) 96, 136
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 2 cut(s) 320, 642
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 336
HapII CCGG 2 cut(s) 129, 425
HgaI GACGC 1 cut(s) 766
Hin1II CATG 7 cut(s) 32, 403, 455, 478, 662, 725, 772
HinfI GANTC 2 cut(s) 73, 663
HpaII CCGG 2 cut(s) 129, 425
HphI GGTGA 5 cut(s) 52, 206, 526, 595, 623
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 4 cut(s) 78, 141, 148, 373
Hpy188III TCNNGA 1 cut(s) 325
Hpy8I GTNNAC 1 cut(s) 565
HpyAV CCTTC 1 cut(s) 580
HpyCH4V TGCA 2 cut(s) 86, 386
HpyF3I CTNAG 3 cut(s) 77, 489, 702
Hsp92II CATG 7 cut(s) 32, 403, 455, 478, 662, 725, 772
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 1 cut(s) 544
LmnI GCTCC 5 cut(s) 131, 205, 374, 589, 703
Lsp1109I GCAGC 1 cut(s) 70
MaeI CTAG 2 cut(s) 320, 642
MaeIII GTNAC 2 cut(s) 253, 762
MalI GATC 1 cut(s) 546
MboI GATC 1 cut(s) 544
MboII GAAGA 3 cut(s) 161, 344, 590
MfeI CAATTG 1 cut(s) 500
MhlI GDGCHC 2 cut(s) 96, 136
MluCI AATT 8 cut(s) 52, 153, 165, 199, 210, 500, 526, 673
MlyI GAGTC 1 cut(s) 67
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 5 cut(s) 68, 553, 574, 599, 660
MseI TTAA 1 cut(s) 746
MslI CAYNNNNRTG 4 cut(s) 68, 657, 726, 773
MspI CCGG 2 cut(s) 129, 425
MspR9I CCNGG 2 cut(s) 129, 711
MunI CAATTG 1 cut(s) 500
MvaI CCWGG 1 cut(s) 711
NciI CCSGG 1 cut(s) 129
NcoI CCATGG 2 cut(s) 451, 658
NdeII GATC 1 cut(s) 544
NlaIII CATG 7 cut(s) 32, 403, 455, 478, 662, 725, 772
NlaIV GGNNCC 2 cut(s) 127, 370
NmuCI GTSAC 1 cut(s) 762
NspI RCATGY 3 cut(s) 403, 478, 725
NspV TTCGAA 1 cut(s) 576
PaqCI CACCTGC 1 cut(s) 182
PciI ACATGT 1 cut(s) 474
PfeI GAWTC 1 cut(s) 663
PflFI GACNNNGTC 1 cut(s) 760
PfoI TCCNGGA 1 cut(s) 127
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
PpuMI RGGWCCY 1 cut(s) 125
PscI ACATGT 1 cut(s) 474
Psp124BI GAGCTC 2 cut(s) 96, 136
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 1 cut(s) 709
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 2 cut(s) 127, 370
PspPI GGNCC 1 cut(s) 125
PspPPI RGGWCCY 1 cut(s) 125
PstI CTGCAG 1 cut(s) 88
PsyI GACNNNGTC 1 cut(s) 760
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 4 cut(s) 68, 657, 726, 773
SacI GAGCTC 2 cut(s) 96, 136
SaqAI TTAA 1 cut(s) 746
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 1 cut(s) 544
Sau96I GGNCC 1 cut(s) 125
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 2 cut(s) 129, 711
SduI GDGCHC 2 cut(s) 96, 136
SfcI CTRYAG 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 576
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 4 cut(s) 68, 657, 726, 773
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 8 cut(s) 52, 153, 165, 199, 210, 500, 526, 673
SspMI CTAG 2 cut(s) 320, 642
SstI GAGCTC 2 cut(s) 96, 136
StyD4I CCNGG 2 cut(s) 127, 709
StyI CCWWGG 2 cut(s) 451, 658
TaqI TCGA 1 cut(s) 576
TasI AATT 8 cut(s) 52, 153, 165, 199, 210, 500, 526, 673
TatI WGTACW 1 cut(s) 408
TfiI GAWTC 1 cut(s) 663
Tru1I TTAA 1 cut(s) 746
Tru9I TTAA 1 cut(s) 746
TseFI GTSAC 1 cut(s) 762
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 762
TspDTI ATGAA 4 cut(s) 221, 345, 778, 787
Tth111I GACNNNGTC 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 5 cut(s) 52, 165, 199, 210, 526
XceI RCATGY 3 cut(s) 403, 478, 725
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XspI CTAG 2 cut(s) 320, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.