Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 1040, 1080 |
| AclWI | GGATC | 1 cut(s) | 1099 |
| AcsI | RAATTY | 5 cut(s) | 52, 165, 199, 529, 989 |
| AcuI | CTGAAG | 2 cut(s) | 18, 1094 |
| AfaI | GTAC | 2 cut(s) | 413, 786 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 796, 854 |
| AflIII | ACRYGT | 1 cut(s) | 477 |
| AgsI | TTSAA | 4 cut(s) | 68, 106, 425, 1025 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 478 |
| AhlI | ACTAGT | 1 cut(s) | 895 |
| AjnI | CCWGG | 1 cut(s) | 127 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 676, 708 |
| AluBI | AGCT | 9 cut(s) | 38, 83, 94, 134, 396, 496, 595, 701, 708 |
| AluI | AGCT | 9 cut(s) | 38, 83, 94, 134, 396, 496, 595, 701, 708 |
| Alw21I | GWGCWC | 2 cut(s) | 136, 879 |
| AlwI | GGATC | 1 cut(s) | 1099 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 128 |
| ApeKI | GCWGC | 1 cut(s) | 83 |
| ApoI | RAATTY | 5 cut(s) | 52, 165, 199, 529, 989 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 626, 658, 749, 781 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 395 |
| AspS9I | GGNCC | 1 cut(s) | 125 |
| AsuHPI | GGTGA | 4 cut(s) | 52, 529, 598, 626 |
| AsuII | TTCGAA | 1 cut(s) | 579 |
| AvaII | GGWCC | 1 cut(s) | 125 |
| BanII | GRGCYC | 2 cut(s) | 136, 374 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 383, 415 |
| BauI | CACGAG | 1 cut(s) | 1047 |
| Bbv12I | GWGCWC | 2 cut(s) | 136, 879 |
| BbvI | GCAGC | 1 cut(s) | 70 |
| BccI | CCATC | 5 cut(s) | 65, 332, 514, 592, 611 |
| BciT130I | CCWGG | 1 cut(s) | 129 |
| BciVI | GTATCC | 1 cut(s) | 660 |
| BclI | TGATCA | 1 cut(s) | 547 |
| BcuI | ACTAGT | 1 cut(s) | 895 |
| BfaI | CTAG | 5 cut(s) | 323, 705, 896, 963, 1001 |
| BfmI | CTRYAG | 1 cut(s) | 84 |
| BfuI | GTATCC | 1 cut(s) | 660 |
| BglII | AGATCT | 1 cut(s) | 1032 |
| BisI | GCNGC | 1 cut(s) | 84 |
| BlsI | GCNGC | 1 cut(s) | 85 |
| Bme1390I | CCNGG | 1 cut(s) | 129 |
| Bme18I | GGWCC | 1 cut(s) | 125 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 478 |
| BmgT120I | GGNCC | 1 cut(s) | 125 |
| BmiI | GGNNCC | 1 cut(s) | 373 |
| BmrFI | CCNGG | 1 cut(s) | 129 |
| BpmI | CTGGAG | 1 cut(s) | 150 |
| Bpu10I | CCTNAGC | 1 cut(s) | 492 |
| Bpu14I | TTCGAA | 1 cut(s) | 579 |
| BpuEI | CTTGAG | 1 cut(s) | 392 |
| BsaJI | CCNNGG | 2 cut(s) | 454, 661 |
| BsaWI | WCCGGW | 3 cut(s) | 355, 427, 847 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 796, 854 |
| Bse3DI | GCAATG | 1 cut(s) | 111 |
| BseBI | CCWGG | 1 cut(s) | 129 |
| BseDI | CCNNGG | 2 cut(s) | 454, 661 |
| BseGI | GGATG | 3 cut(s) | 607, 657, 805 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 796, 854 |
| BseMI | GCAATG | 1 cut(s) | 111 |
| BseMII | CTCAG | 2 cut(s) | 68, 483 |
| BseXI | GCAGC | 1 cut(s) | 70 |
| BsiHKAI | GWGCWC | 2 cut(s) | 136, 879 |
| BsiSI | CCGG | 3 cut(s) | 356, 428, 848 |
| BslFI | GGGAC | 1 cut(s) | 311 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 796, 854 |
| BsmFI | GGGAC | 1 cut(s) | 311 |
| BsmI | GAATGC | 1 cut(s) | 216 |
| Bsp119I | TTCGAA | 1 cut(s) | 579 |
| Bsp1286I | GDGCHC | 3 cut(s) | 136, 374, 879 |
| Bsp143I | GATC | 3 cut(s) | 547, 1032, 1091 |
| Bsp19I | CCATGG | 2 cut(s) | 454, 661 |
| BspACI | CCGC | 2 cut(s) | 1040, 1080 |
| BspCNI | CTCAG | 2 cut(s) | 69, 484 |
| BspHI | TCATGA | 1 cut(s) | 945 |
| BspLI | GGNNCC | 1 cut(s) | 373 |
| BspMAI | CTGCAG | 1 cut(s) | 88 |
| BspPI | GGATC | 1 cut(s) | 1099 |
| BspQI | GCTCTTC | 1 cut(s) | 386 |
| BspT104I | TTCGAA | 1 cut(s) | 579 |
| BsrDI | GCAATG | 1 cut(s) | 111 |
| BssECI | CCNNGG | 2 cut(s) | 454, 661 |
| BssMI | GATC | 3 cut(s) | 547, 1032, 1091 |
| BssSI | CACGAG | 1 cut(s) | 1047 |
| BssT1I | CCWWGG | 2 cut(s) | 454, 661 |
| Bst2BI | CACGAG | 1 cut(s) | 1047 |
| Bst2UI | CCWGG | 1 cut(s) | 129 |
| Bst4CI | ACNGT | 3 cut(s) | 507, 812, 833 |
| Bst6I | CTCTTC | 2 cut(s) | 386, 576 |
| BstBI | TTCGAA | 1 cut(s) | 579 |
| BstC8I | GCNNGC | 2 cut(s) | 710, 978 |
| BstDEI | CTNAG | 2 cut(s) | 77, 492 |
| BstDSI | CCRYGG | 2 cut(s) | 454, 661 |
| BstF5I | GGATG | 3 cut(s) | 607, 657, 805 |
| BstKTI | GATC | 3 cut(s) | 550, 1035, 1094 |
| BstMBI | GATC | 3 cut(s) | 547, 1032, 1091 |
| BstNI | CCWGG | 1 cut(s) | 129 |
| BstNSI | RCATGY | 4 cut(s) | 195, 406, 481, 728 |
| BstSCI | CCNGG | 1 cut(s) | 127 |
| BstSFI | CTRYAG | 1 cut(s) | 84 |
| BstV1I | GCAGC | 1 cut(s) | 70 |
| BstX2I | RGATCY | 1 cut(s) | 1032 |
| BstYI | RGATCY | 1 cut(s) | 1032 |
| BsuI | GTATCC | 1 cut(s) | 660 |
| BtgI | CCRYGG | 2 cut(s) | 454, 661 |
| BtsCI | GGATG | 3 cut(s) | 607, 657, 805 |
| BtsIMutI | CAGTG | 1 cut(s) | 808 |
| Cac8I | GCNNGC | 2 cut(s) | 710, 978 |
| CaiI | CAGNNNCTG | 1 cut(s) | 128 |
| CciI | TCATGA | 1 cut(s) | 945 |
| Cfr13I | GGNCC | 1 cut(s) | 125 |
| CseI | GACGC | 1 cut(s) | 769 |
| Csp6I | GTAC | 2 cut(s) | 412, 785 |
| CviQI | GTAC | 2 cut(s) | 412, 785 |
| DdeI | CTNAG | 2 cut(s) | 77, 492 |
| DpnI | GATC | 3 cut(s) | 549, 1034, 1093 |
| DpnII | GATC | 3 cut(s) | 547, 1032, 1091 |
| DriI | GACNNNNNGTC | 1 cut(s) | 478 |
| Eam1104I | CTCTTC | 2 cut(s) | 386, 576 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 478 |
| EarI | CTCTTC | 2 cut(s) | 386, 576 |
| Ecl136II | GAGCTC | 1 cut(s) | 134 |
| Eco130I | CCWWGG | 2 cut(s) | 454, 661 |
| Eco24I | GRGCYC | 2 cut(s) | 136, 374 |
| Eco32I | GATATC | 1 cut(s) | 997 |
| Eco47I | GGWCC | 1 cut(s) | 125 |
| Eco53kI | GAGCTC | 1 cut(s) | 134 |
| Eco57I | CTGAAG | 2 cut(s) | 18, 1094 |
| EcoICRI | GAGCTC | 1 cut(s) | 134 |
| EcoO109I | RGGNCCY | 1 cut(s) | 125 |
| EcoRII | CCWGG | 1 cut(s) | 127 |
| EcoRV | GATATC | 1 cut(s) | 997 |
| EcoT14I | CCWWGG | 2 cut(s) | 454, 661 |
| EcoT38I | GRGCYC | 2 cut(s) | 136, 374 |
| ErhI | CCWWGG | 2 cut(s) | 454, 661 |
| FaqI | GGGAC | 1 cut(s) | 311 |
| FauI | CCCGC | 2 cut(s) | 1047, 1073 |
| FbaI | TGATCA | 1 cut(s) | 547 |
| Fnu4HI | GCNGC | 1 cut(s) | 84 |
| FokI | GGATG | 3 cut(s) | 614, 644, 812 |
| FriOI | GRGCYC | 2 cut(s) | 136, 374 |
| Fsp4HI | GCNGC | 1 cut(s) | 84 |
| FspBI | CTAG | 5 cut(s) | 323, 705, 896, 963, 1001 |
| GluI | GCNGC | 1 cut(s) | 84 |
| GsuI | CTGGAG | 1 cut(s) | 150 |
| HapII | CCGG | 3 cut(s) | 356, 428, 848 |
| HgaI | GACGC | 1 cut(s) | 769 |
| HinfI | GANTC | 4 cut(s) | 73, 331, 666, 1054 |
| HpaII | CCGG | 3 cut(s) | 356, 428, 848 |
| HphI | GGTGA | 4 cut(s) | 52, 529, 598, 626 |
| Hpy166II | GTNNAC | 1 cut(s) | 568 |
| Hpy188I | TCNGA | 4 cut(s) | 78, 141, 376, 1074 |
| Hpy188III | TCNNGA | 3 cut(s) | 328, 335, 946 |
| Hpy8I | GTNNAC | 1 cut(s) | 568 |
| HpyAV | CCTTC | 3 cut(s) | 583, 853, 1069 |
| HpyCH4III | ACNGT | 3 cut(s) | 507, 812, 833 |
| HpyCH4V | TGCA | 2 cut(s) | 86, 712 |
| HpyF3I | CTNAG | 2 cut(s) | 77, 492 |
| Ksp22I | TGATCA | 1 cut(s) | 547 |
| Kzo9I | GATC | 3 cut(s) | 547, 1032, 1091 |
| LguI | GCTCTTC | 1 cut(s) | 386 |
| LmnI | GCTCC | 9 cut(s) | 131, 205, 358, 377, 592, 706, 721, 973, 1083 |
| LpnPI | CCDG | 8 cut(s) | 108, 114, 141, 369, 441, 504, 861, 903 |
| Lsp1109I | GCAGC | 1 cut(s) | 70 |
| MaeI | CTAG | 5 cut(s) | 323, 705, 896, 963, 1001 |
| MalI | GATC | 3 cut(s) | 549, 1034, 1093 |
| MboI | GATC | 3 cut(s) | 547, 1032, 1091 |
| MboII | GAAGA | 5 cut(s) | 403, 593, 646, 829, 1027 |
| MflI | RGATCY | 1 cut(s) | 1032 |
| MhlI | GDGCHC | 3 cut(s) | 136, 374, 879 |
| MluCI | AATT | 8 cut(s) | 52, 153, 165, 199, 529, 793, 864, 989 |
| MlyI | GAGTC | 2 cut(s) | 67, 1063 |
| MmeI | TCCRAC | 1 cut(s) | 206 |
| MnlI | CCTC | 6 cut(s) | 556, 577, 602, 663, 832, 1056 |
| MroXI | GAANNNNTTC | 1 cut(s) | 395 |
| MseI | TTAA | 3 cut(s) | 96, 254, 749 |
| MslI | CAYNNNNRTG | 5 cut(s) | 68, 660, 729, 776, 944 |
| MspI | CCGG | 3 cut(s) | 356, 428, 848 |
| MspR9I | CCNGG | 1 cut(s) | 129 |
| Mva1269I | GAATGC | 1 cut(s) | 216 |
| MvaI | CCWGG | 1 cut(s) | 129 |
| NcoI | CCATGG | 2 cut(s) | 454, 661 |
| NdeII | GATC | 3 cut(s) | 547, 1032, 1091 |
| NlaIV | GGNNCC | 1 cut(s) | 373 |
| NspI | RCATGY | 4 cut(s) | 195, 406, 481, 728 |
| NspV | TTCGAA | 1 cut(s) | 579 |
| PagI | TCATGA | 1 cut(s) | 945 |
| PciI | ACATGT | 1 cut(s) | 477 |
| PciSI | GCTCTTC | 1 cut(s) | 386 |
| PctI | GAATGC | 1 cut(s) | 216 |
| PdmI | GAANNNNTTC | 1 cut(s) | 395 |
| PfeI | GAWTC | 2 cut(s) | 331, 666 |
| PflFI | GACNNNGTC | 1 cut(s) | 763 |
| PfoI | TCCNGGA | 1 cut(s) | 127 |
| PkrI | GCNGC | 1 cut(s) | 85 |
| PleI | GAGTC | 2 cut(s) | 67, 1062 |
| PpsI | GAGTC | 2 cut(s) | 67, 1062 |
| PpuMI | RGGWCCY | 1 cut(s) | 125 |
| PscI | ACATGT | 1 cut(s) | 477 |
| Psp124BI | GAGCTC | 1 cut(s) | 136 |
| Psp5II | RGGWCCY | 1 cut(s) | 125 |
| Psp6I | CCWGG | 1 cut(s) | 127 |
| PspGI | CCWGG | 1 cut(s) | 127 |
| PspN4I | GGNNCC | 1 cut(s) | 373 |
| PspPI | GGNCC | 1 cut(s) | 125 |
| PspPPI | RGGWCCY | 1 cut(s) | 125 |
| PstI | CTGCAG | 1 cut(s) | 88 |
| PstNI | CAGNNNCTG | 1 cut(s) | 128 |
| PsuI | RGATCY | 1 cut(s) | 1032 |
| PsyI | GACNNNGTC | 1 cut(s) | 763 |
| RsaI | GTAC | 2 cut(s) | 413, 786 |
| RsaNI | GTAC | 2 cut(s) | 412, 785 |
| RseI | CAYNNNNRTG | 5 cut(s) | 68, 660, 729, 776, 944 |
| SacI | GAGCTC | 1 cut(s) | 136 |
| SapI | GCTCTTC | 1 cut(s) | 386 |
| SaqAI | TTAA | 3 cut(s) | 96, 254, 749 |
| SatI | GCNGC | 1 cut(s) | 84 |
| Sau3AI | GATC | 3 cut(s) | 547, 1032, 1091 |
| Sau96I | GGNCC | 1 cut(s) | 125 |
| SchI | GAGTC | 2 cut(s) | 67, 1063 |
| ScrFI | CCNGG | 1 cut(s) | 129 |
| SduI | GDGCHC | 3 cut(s) | 136, 374, 879 |
| SfcI | CTRYAG | 1 cut(s) | 84 |
| SfuI | TTCGAA | 1 cut(s) | 579 |
| SinI | GGWCC | 1 cut(s) | 125 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 68, 660, 729, 776, 944 |
| SmlI | CTYRAG | 1 cut(s) | 407 |
| SmoI | CTYRAG | 1 cut(s) | 407 |
| SpeI | ACTAGT | 1 cut(s) | 895 |
| Sse9I | AATT | 8 cut(s) | 52, 153, 165, 199, 529, 793, 864, 989 |
| SsiI | CCGC | 2 cut(s) | 1040, 1080 |
| SspMI | CTAG | 5 cut(s) | 323, 705, 896, 963, 1001 |
| SstI | GAGCTC | 1 cut(s) | 136 |
| StyD4I | CCNGG | 1 cut(s) | 127 |
| StyI | CCWWGG | 2 cut(s) | 454, 661 |
| TaaI | ACNGT | 3 cut(s) | 507, 812, 833 |
| TaqI | TCGA | 2 cut(s) | 579, 675 |
| TasI | AATT | 8 cut(s) | 52, 153, 165, 199, 529, 793, 864, 989 |
| TatI | WGTACW | 1 cut(s) | 411 |
| TfiI | GAWTC | 2 cut(s) | 331, 666 |
| Tru1I | TTAA | 3 cut(s) | 96, 254, 749 |
| Tru9I | TTAA | 3 cut(s) | 96, 254, 749 |
| TscAI | CASTG | 1 cut(s) | 815 |
| TseI | GCWGC | 1 cut(s) | 83 |
| TspDTI | ATGAA | 4 cut(s) | 221, 819, 932, 934 |
| TspGWI | ACGGA | 1 cut(s) | 1000 |
| TspRI | CASTG | 1 cut(s) | 815 |
| Tth111I | GACNNNGTC | 1 cut(s) | 763 |
| VpaK11BI | GGWCC | 1 cut(s) | 125 |
| XapI | RAATTY | 5 cut(s) | 52, 165, 199, 529, 989 |
| XceI | RCATGY | 4 cut(s) | 195, 406, 481, 728 |
| XmnI | GAANNNNTTC | 1 cut(s) | 395 |
| XspI | CTAG | 5 cut(s) | 323, 705, 896, 963, 1001 |