Rh5DG396100
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000656
Physical Location & Seq
Forward (+)
62222910 .. 62225502
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG396100.1

Sequence Viewer

Length: 1101 bp
ATGGAAATCCAGAGAATTAGCTCAAAGGACTATGTATCTGCTTGGAATTTGGGTGGTCTAGTTGTCACTCAAATGGCTTTAAAAGCTGCAATCGAGCTCAATGTCTTCAATATCATTGCCAATTCAGGACCTGGAGCTCATCTCACATTGAAAGAAATTGTATCTCAAATCTCAAATACAAATACAAATCCAAATGCAGCAGCTGCAAATTTGAAGCGAATACTAAGAGTTCTCAGTGTTCATTCTCTGCTATCTGTATCTCAAAGGTCAAGCTCAAGTGGTAAGACAATGCTAGAAGAGACCTATGGTCTAACAAATGAGACTCTTTGCCTAATTCCAAATGAAGATGGAGTTTCCTTAGCCCCATATATTTTGTTAGCTTCAGAAATGCTGACTGTGAAATGCTACTCCATGCTCAAAGACACAGTGCTTGAGCCTGGGAGCTTACCTTTCAAGAAGGCTCATGGCATGTCCGCATATGAATACACGTTCGGGAAACCTGAGCAAACAAATTTGTTTAATGAGGCTATAGGCCAAAGTACCATCATCAATTTTGAGGATGTGTTAGAGGTTTACAAAGGTTTTGAAGAGGTAAAAGAGTTGATGGATGTTGGAGGAGGCAATGGAACTACAATTGCCAAAGTAATATCTAGTTATCCACACATTCATGGGATTAACTTTGATTTGCCTAATGTTATCGCTCAAGCAACTGCTAAGTATCAAGCAGGTGTGAAATATGTTGGTGGAGATATGTTTGAGTTGATACCAAATGCACAATCCATTATGTTGAAGTGGGTGCTTCATAATTGGGATGATGATCTATGCAATAAAATATTAGAACGTTGTTGGGAGGCATTACCTGAAATTGGGAAGGTGATAGTTGTGGAATTTGTAATACCCGAAATACTGGAGAAAACCACAGACTTAAAGAAAATAGTGGCATTAGACATCATGATGATGAGTATTTGTGGTGGCAAGCAACGAACAATTACTGAGTTTGACGGCCTATCGAAAGCTGCAGGCTTTGTTGAAACAAAAATTTTTCCGATTTCACATGGGTGTTATGTTATGGAGTTTCACAAAGTTAAGAAAAATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

366

Amino Acids

40.37

Weight (kDa)

5.82

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 21 - 105 2.6e-11 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 136 - 342 3.2e-56 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 716
Acc36I ACCTGC 1 cut(s) 716
AciI CCGC 1 cut(s) 474
AclI AACGTT 1 cut(s) 841
AcsI RAATTY 5 cut(s) 46, 208, 511, 887, 1038
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 1 cut(s) 541
AfiI CCNNNNNNNGG 1 cut(s) 866
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 7 cut(s) 109, 151, 214, 454, 587, 790, 1031
AhdI GACNNNNNGTC 1 cut(s) 306
AjnI CCWGG 2 cut(s) 130, 436
AleI CACNNNNGTG 1 cut(s) 1057
AluBI AGCT 9 cut(s) 21, 86, 97, 137, 203, 273, 380, 444, 1016
AluI AGCT 9 cut(s) 21, 86, 97, 137, 203, 273, 380, 444, 1016
Alw21I GWGCWC 2 cut(s) 99, 139
Alw26I GTCTC 2 cut(s) 293, 314
AlwNI CAGNNNCTG 2 cut(s) 131, 203
AoxI GGCC 2 cut(s) 532, 1003
ApeKI GCWGC 5 cut(s) 86, 197, 200, 203, 1016
ApoI RAATTY 5 cut(s) 46, 208, 511, 887, 1038
AspS9I GGNCC 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 886
AvaII GGWCC 1 cut(s) 128
BanII GRGCYC 2 cut(s) 99, 139
BbsI GAAGAC 1 cut(s) 97
Bbv12I GWGCWC 2 cut(s) 99, 139
BbvI GCAGC 5 cut(s) 73, 190, 209, 212, 1003
BccI CCATC 3 cut(s) 341, 551, 598
BceAI ACGGC 1 cut(s) 1018
BciT130I CCWGG 2 cut(s) 132, 438
BcoDI GTCTC 2 cut(s) 293, 314
BfaI CTAG 3 cut(s) 59, 293, 651
BfmI CTRYAG 2 cut(s) 528, 1017
BfuAI ACCTGC 1 cut(s) 716
BisI GCNGC 5 cut(s) 87, 198, 201, 204, 1017
BlsI GCNGC 5 cut(s) 88, 199, 202, 205, 1018
Bme1390I CCNGG 2 cut(s) 132, 438
Bme18I GGWCC 1 cut(s) 128
BmeRI GACNNNNNGTC 1 cut(s) 306
BmgT120I GGNCC 1 cut(s) 128
BmrFI CCNGG 2 cut(s) 132, 438
BpiI GAAGAC 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 126, 158
BpmI CTGGAG 2 cut(s) 153, 929
Bpu10I CCTNAGC 2 cut(s) 358, 501
BpuEI CTTGAG 3 cut(s) 259, 452, 687
BsaBI GATNNNNATC 1 cut(s) 816
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 437
Bsc4I CCNNNNNNNGG 1 cut(s) 866
Bse1I ACTGG 1 cut(s) 912
Bse3DI GCAATG 2 cut(s) 114, 628
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 2 cut(s) 132, 438
BseDI CCNNGG 1 cut(s) 437
BseGI GGATG 3 cut(s) 565, 613, 817
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 1 cut(s) 866
BseMI GCAATG 2 cut(s) 114, 628
BseMII CTCAG 3 cut(s) 247, 492, 984
BseNI ACTGG 1 cut(s) 912
BseRI GAGGAG 1 cut(s) 630
BseXI GCAGC 5 cut(s) 73, 190, 209, 212, 1003
BshFI GGCC 2 cut(s) 534, 1005
BsiHKAI GWGCWC 2 cut(s) 99, 139
BslI CCNNNNNNNGG 1 cut(s) 866
BsmAI GTCTC 2 cut(s) 293, 314
BsnI GGCC 2 cut(s) 534, 1005
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 2 cut(s) 99, 139
Bsp143I GATC 1 cut(s) 817
BspACI CCGC 1 cut(s) 474
BspANI GGCC 2 cut(s) 534, 1005
BspCNI CTCAG 3 cut(s) 246, 493, 985
BspHI TCATGA 1 cut(s) 951
BspMAI CTGCAG 1 cut(s) 1021
BspMI ACCTGC 1 cut(s) 716
BspTNI GGTCTC 1 cut(s) 293
BsrDI GCAATG 2 cut(s) 114, 628
BsrI ACTGG 1 cut(s) 912
BssECI CCNNGG 1 cut(s) 437
BssMI GATC 1 cut(s) 817
Bst2UI CCWGG 2 cut(s) 132, 438
Bst4CI ACNGT 2 cut(s) 397, 427
Bst6I CTCTTC 2 cut(s) 291, 582
BstAPI GCANNNNNTGC 1 cut(s) 203
BstC8I GCNNGC 2 cut(s) 977, 1021
BstDEI CTNAG 6 cut(s) 224, 233, 358, 501, 714, 993
BstF5I GGATG 3 cut(s) 565, 613, 817
BstKTI GATC 1 cut(s) 820
BstMAI GTCTC 2 cut(s) 293, 314
BstMBI GATC 1 cut(s) 817
BstMWI GCNNNNNNNGC 2 cut(s) 83, 203
BstNI CCWGG 2 cut(s) 132, 438
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 2 cut(s) 130, 436
BstSFI CTRYAG 2 cut(s) 528, 1017
BstV1I GCAGC 5 cut(s) 73, 190, 209, 212, 1003
BstV2I GAAGAC 1 cut(s) 97
BsuRI GGCC 2 cut(s) 534, 1005
BtsCI GGATG 3 cut(s) 565, 613, 817
BtsIMutI CAGTG 2 cut(s) 241, 432
BveI ACCTGC 1 cut(s) 716
Cac8I GCNNGC 2 cut(s) 977, 1021
CaiI CAGNNNCTG 2 cut(s) 131, 203
CciI TCATGA 1 cut(s) 951
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 540
CviAII CATG 6 cut(s) 412, 464, 469, 668, 952, 1055
CviQI GTAC 1 cut(s) 540
DdeI CTNAG 6 cut(s) 224, 233, 358, 501, 714, 993
DpnI GATC 1 cut(s) 819
DpnII GATC 1 cut(s) 817
DraI TTTAAA 1 cut(s) 81
DriI GACNNNNNGTC 1 cut(s) 306
Eam1104I CTCTTC 2 cut(s) 291, 582
Eam1105I GACNNNNNGTC 1 cut(s) 306
EarI CTCTTC 2 cut(s) 291, 582
Ecl136II GAGCTC 2 cut(s) 97, 137
Eco24I GRGCYC 2 cut(s) 99, 139
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 128
Eco53kI GAGCTC 2 cut(s) 97, 137
Eco57I CTGAAG 1 cut(s) 366
EcoICRI GAGCTC 2 cut(s) 97, 137
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 130, 436
EcoT38I GRGCYC 2 cut(s) 99, 139
FaeI CATG 6 cut(s) 415, 467, 472, 671, 955, 1058
FatI CATG 6 cut(s) 411, 463, 468, 667, 951, 1054
FauNDI CATATG 1 cut(s) 478
Fnu4HI GCNGC 5 cut(s) 87, 198, 201, 204, 1017
FokI GGATG 3 cut(s) 572, 620, 824
FriOI GRGCYC 2 cut(s) 99, 139
Fsp4HI GCNGC 5 cut(s) 87, 198, 201, 204, 1017
FspBI CTAG 3 cut(s) 59, 293, 651
GluI GCNGC 5 cut(s) 87, 198, 201, 204, 1017
GsuI CTGGAG 2 cut(s) 153, 929
HaeIII GGCC 2 cut(s) 534, 1005
Hin1II CATG 6 cut(s) 415, 467, 472, 671, 955, 1058
HinfI GANTC 1 cut(s) 322
HphI GGTGA 1 cut(s) 886
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 2 cut(s) 385, 1047
Hpy188III TCNNGA 5 cut(s) 10, 126, 454, 493, 952
Hpy8I GTNNAC 1 cut(s) 574
HpyAV CCTTC 2 cut(s) 451, 865
HpyCH4III ACNGT 2 cut(s) 397, 427
HpyCH4IV ACGT 2 cut(s) 488, 841
HpyCH4V TGCA 6 cut(s) 89, 197, 206, 773, 825, 1019
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 203
HpyF3I CTNAG 6 cut(s) 224, 233, 358, 501, 714, 993
HpySE526I ACGT 2 cut(s) 488, 841
Hsp92II CATG 6 cut(s) 415, 467, 472, 671, 955, 1058
Kzo9I GATC 1 cut(s) 817
LmnI GCTCC 2 cut(s) 134, 441
Lsp1109I GCAGC 5 cut(s) 73, 190, 209, 212, 1003
MaeI CTAG 3 cut(s) 59, 293, 651
MaeII ACGT 2 cut(s) 488, 841
MaeIII GTNAC 1 cut(s) 64
MalI GATC 1 cut(s) 819
MboI GATC 1 cut(s) 817
MboII GAAGA 4 cut(s) 97, 308, 356, 599
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 2 cut(s) 99, 139
MlyI GAGTC 1 cut(s) 316
MmeI TCCRAC 1 cut(s) 592
MnlI CCTC 7 cut(s) 517, 550, 562, 583, 608, 611, 844
MseI TTAA 5 cut(s) 80, 519, 675, 926, 1086
MslI CAYNNNNRTG 5 cut(s) 71, 666, 953, 956, 1057
MspA1I CMGCKG 1 cut(s) 203
MspR9I CCNGG 2 cut(s) 132, 438
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 2 cut(s) 132, 438
MwoI GCNNNNNNNGC 2 cut(s) 83, 203
NdeI CATATG 1 cut(s) 478
NdeII GATC 1 cut(s) 817
NlaIII CATG 6 cut(s) 415, 467, 472, 671, 955, 1058
NmuCI GTSAC 1 cut(s) 64
NspI RCATGY 1 cut(s) 472
OliI CACNNNNGTG 1 cut(s) 1057
PagI TCATGA 1 cut(s) 951
PaqCI CACCTGC 1 cut(s) 716
PkrI GCNGC 5 cut(s) 88, 199, 202, 205, 1018
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 128
Psp124BI GAGCTC 2 cut(s) 99, 139
Psp1406I AACGTT 1 cut(s) 841
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 2 cut(s) 130, 436
PspGI CCWGG 2 cut(s) 130, 436
PspPI GGNCC 1 cut(s) 128
PspPPI RGGWCCY 1 cut(s) 128
PstI CTGCAG 1 cut(s) 1021
PstNI CAGNNNCTG 2 cut(s) 131, 203
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 1 cut(s) 541
RsaNI GTAC 1 cut(s) 540
RseI CAYNNNNRTG 5 cut(s) 71, 666, 953, 956, 1057
SacI GAGCTC 2 cut(s) 99, 139
SaqAI TTAA 5 cut(s) 80, 519, 675, 926, 1086
SatI GCNGC 5 cut(s) 87, 198, 201, 204, 1017
Sau3AI GATC 1 cut(s) 817
Sau96I GGNCC 1 cut(s) 128
SchI GAGTC 1 cut(s) 316
ScrFI CCNGG 2 cut(s) 132, 438
SduI GDGCHC 2 cut(s) 99, 139
SfcI CTRYAG 2 cut(s) 528, 1017
SinI GGWCC 1 cut(s) 128
SmiMI CAYNNNNRTG 5 cut(s) 71, 666, 953, 956, 1057
SmlI CTYRAG 3 cut(s) 274, 431, 702
SmoI CTYRAG 3 cut(s) 274, 431, 702
SsiI CCGC 1 cut(s) 474
SspI AATATT 1 cut(s) 834
SspMI CTAG 3 cut(s) 59, 293, 651
SstI GAGCTC 2 cut(s) 99, 139
StyD4I CCNGG 2 cut(s) 130, 436
TaaI ACNGT 2 cut(s) 397, 427
TaiI ACGT 2 cut(s) 491, 844
TaqI TCGA 2 cut(s) 93, 1010
Tru1I TTAA 5 cut(s) 80, 519, 675, 926, 1086
Tru9I TTAA 5 cut(s) 80, 519, 675, 926, 1086
TscAI CASTG 2 cut(s) 241, 432
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 5 cut(s) 86, 197, 200, 203, 1016
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 5 cut(s) 230, 357, 495, 656, 791
TspRI CASTG 2 cut(s) 241, 432
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 5 cut(s) 46, 208, 511, 887, 1038
XceI RCATGY 1 cut(s) 472
XspI CTAG 3 cut(s) 59, 293, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.