Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 4 cut(s) | 52, 199, 449, 463 |
| AdeI | CACNNNGTG | 1 cut(s) | 418 |
| AfaI | GTAC | 1 cut(s) | 786 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 854 |
| AflII | CTTAAG | 2 cut(s) | 215, 560 |
| AflIII | ACRYGT | 2 cut(s) | 477, 738 |
| AgsI | TTSAA | 7 cut(s) | 68, 106, 511, 581, 676, 778, 1022 |
| Alw21I | GWGCWC | 2 cut(s) | 96, 810 |
| Alw26I | GTCTC | 3 cut(s) | 37, 481, 729 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 983 |
| AoxI | GGCC | 2 cut(s) | 125, 298 |
| ApeKI | GCWGC | 3 cut(s) | 83, 191, 325 |
| ApoI | RAATTY | 4 cut(s) | 52, 199, 449, 463 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 626, 658 |
| AspS9I | GGNCC | 1 cut(s) | 125 |
| AsuHPI | GGTGA | 2 cut(s) | 626, 947 |
| BanII | GRGCYC | 2 cut(s) | 96, 374 |
| BbsI | GAAGAC | 1 cut(s) | 94 |
| Bbv12I | GWGCWC | 2 cut(s) | 96, 810 |
| BbvI | GCAGC | 3 cut(s) | 70, 203, 312 |
| BccI | CCATC | 3 cut(s) | 332, 611, 943 |
| BciVI | GTATCC | 1 cut(s) | 660 |
| BclI | TGATCA | 1 cut(s) | 547 |
| BcoDI | GTCTC | 3 cut(s) | 37, 481, 729 |
| BfaI | CTAG | 2 cut(s) | 684, 998 |
| BfrI | CTTAAG | 2 cut(s) | 215, 560 |
| BfuI | GTATCC | 1 cut(s) | 660 |
| BglII | AGATCT | 1 cut(s) | 495 |
| BisI | GCNGC | 3 cut(s) | 84, 192, 326 |
| BlsI | GCNGC | 3 cut(s) | 85, 193, 327 |
| BmgT120I | GGNCC | 1 cut(s) | 125 |
| BmrI | ACTGGG | 1 cut(s) | 805 |
| BmsI | GCATC | 1 cut(s) | 675 |
| BmuI | ACTGGG | 1 cut(s) | 805 |
| BpiI | GAAGAC | 1 cut(s) | 94 |
| BpuEI | CTTGAG | 4 cut(s) | 392, 443, 681, 1055 |
| BsaI | GGTCTC | 1 cut(s) | 481 |
| BsaJI | CCNNGG | 2 cut(s) | 10, 661 |
| BsaWI | WCCGGW | 1 cut(s) | 854 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 854 |
| Bse1I | ACTGG | 1 cut(s) | 800 |
| Bse3DI | GCAATG | 1 cut(s) | 111 |
| BseDI | CCNNGG | 2 cut(s) | 10, 661 |
| BseGI | GGATG | 3 cut(s) | 607, 657, 805 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 854 |
| BseMI | GCAATG | 1 cut(s) | 111 |
| BseMII | CTCAG | 3 cut(s) | 48, 429, 483 |
| BseNI | ACTGG | 1 cut(s) | 800 |
| BseXI | GCAGC | 3 cut(s) | 70, 203, 312 |
| BshFI | GGCC | 2 cut(s) | 127, 300 |
| BsiHKAI | GWGCWC | 2 cut(s) | 96, 810 |
| BsiSI | CCGG | 1 cut(s) | 855 |
| BslFI | GGGAC | 1 cut(s) | 466 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 854 |
| BsmAI | GTCTC | 3 cut(s) | 37, 481, 729 |
| BsmFI | GGGAC | 1 cut(s) | 466 |
| BsmI | GAATGC | 1 cut(s) | 63 |
| BsnI | GGCC | 2 cut(s) | 127, 300 |
| Bso31I | GGTCTC | 1 cut(s) | 481 |
| Bsp1286I | GDGCHC | 3 cut(s) | 96, 374, 810 |
| Bsp143I | GATC | 2 cut(s) | 495, 547 |
| Bsp19I | CCATGG | 1 cut(s) | 661 |
| BspANI | GGCC | 2 cut(s) | 127, 300 |
| BspCNI | CTCAG | 3 cut(s) | 47, 428, 484 |
| BspHI | TCATGA | 1 cut(s) | 519 |
| BspTI | CTTAAG | 2 cut(s) | 215, 560 |
| BspTNI | GGTCTC | 1 cut(s) | 481 |
| BsrDI | GCAATG | 1 cut(s) | 111 |
| BsrI | ACTGG | 1 cut(s) | 800 |
| BssECI | CCNNGG | 2 cut(s) | 10, 661 |
| BssMI | GATC | 2 cut(s) | 495, 547 |
| BssT1I | CCWWGG | 2 cut(s) | 10, 661 |
| Bst4CI | ACNGT | 3 cut(s) | 812, 833, 979 |
| BstAFI | CTTAAG | 2 cut(s) | 215, 560 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 388, 418 |
| BstC8I | GCNNGC | 1 cut(s) | 323 |
| BstDEI | CTNAG | 7 cut(s) | 34, 77, 117, 195, 224, 415, 492 |
| BstDSI | CCRYGG | 1 cut(s) | 661 |
| BstF5I | GGATG | 3 cut(s) | 607, 657, 805 |
| BstKTI | GATC | 2 cut(s) | 498, 550 |
| BstMAI | GTCTC | 3 cut(s) | 37, 481, 729 |
| BstMBI | GATC | 2 cut(s) | 495, 547 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 80, 388, 418 |
| BstNSI | RCATGY | 4 cut(s) | 406, 481, 728, 742 |
| BstV1I | GCAGC | 3 cut(s) | 70, 203, 312 |
| BstV2I | GAAGAC | 1 cut(s) | 94 |
| BstX2I | RGATCY | 1 cut(s) | 495 |
| BstYI | RGATCY | 1 cut(s) | 495 |
| BsuI | GTATCC | 1 cut(s) | 660 |
| BsuRI | GGCC | 2 cut(s) | 127, 300 |
| BtgI | CCRYGG | 1 cut(s) | 661 |
| BtsCI | GGATG | 3 cut(s) | 607, 657, 805 |
| BtsIMutI | CAGTG | 3 cut(s) | 423, 736, 808 |
| Cac8I | GCNNGC | 1 cut(s) | 323 |
| CaiI | CAGNNNCTG | 1 cut(s) | 983 |
| CciI | TCATGA | 1 cut(s) | 519 |
| Cfr13I | GGNCC | 1 cut(s) | 125 |
| Csp6I | GTAC | 1 cut(s) | 785 |
| CviAII | CATG | 9 cut(s) | 403, 478, 520, 662, 725, 739, 883, 1037, 1046 |
| CviQI | GTAC | 1 cut(s) | 785 |
| DdeI | CTNAG | 7 cut(s) | 34, 77, 117, 195, 224, 415, 492 |
| DpnI | GATC | 2 cut(s) | 497, 549 |
| DpnII | GATC | 2 cut(s) | 495, 547 |
| DraIII | CACNNNGTG | 1 cut(s) | 418 |
| Ecl136II | GAGCTC | 1 cut(s) | 94 |
| Eco130I | CCWWGG | 2 cut(s) | 10, 661 |
| Eco24I | GRGCYC | 2 cut(s) | 96, 374 |
| Eco31I | GGTCTC | 1 cut(s) | 481 |
| Eco53kI | GAGCTC | 1 cut(s) | 94 |
| EcoICRI | GAGCTC | 1 cut(s) | 94 |
| EcoT14I | CCWWGG | 2 cut(s) | 10, 661 |
| EcoT38I | GRGCYC | 2 cut(s) | 96, 374 |
| ErhI | CCWWGG | 2 cut(s) | 10, 661 |
| FaeI | CATG | 9 cut(s) | 406, 481, 523, 665, 728, 742, 886, 1040, 1049 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 123, 155 |
| FaqI | GGGAC | 1 cut(s) | 466 |
| FatI | CATG | 9 cut(s) | 402, 477, 519, 661, 724, 738, 882, 1036, 1045 |
| FbaI | TGATCA | 1 cut(s) | 547 |
| Fnu4HI | GCNGC | 3 cut(s) | 84, 192, 326 |
| FokI | GGATG | 3 cut(s) | 614, 644, 812 |
| FriOI | GRGCYC | 2 cut(s) | 96, 374 |
| Fsp4HI | GCNGC | 3 cut(s) | 84, 192, 326 |
| FspBI | CTAG | 2 cut(s) | 684, 998 |
| GluI | GCNGC | 3 cut(s) | 84, 192, 326 |
| HaeIII | GGCC | 2 cut(s) | 127, 300 |
| HapII | CCGG | 1 cut(s) | 855 |
| Hin1II | CATG | 9 cut(s) | 406, 481, 523, 665, 728, 742, 886, 1040, 1049 |
| HindIII | AAGCTT | 1 cut(s) | 218 |
| HinfI | GANTC | 2 cut(s) | 210, 746 |
| HpaII | CCGG | 1 cut(s) | 855 |
| HphI | GGTGA | 2 cut(s) | 626, 947 |
| Hpy188I | TCNGA | 2 cut(s) | 376, 552 |
| Hpy188III | TCNNGA | 4 cut(s) | 20, 520, 890, 1072 |
| HpyAV | CCTTC | 1 cut(s) | 547 |
| HpyCH4III | ACNGT | 3 cut(s) | 812, 833, 979 |
| HpyCH4IV | ACGT | 2 cut(s) | 384, 939 |
| HpyCH4V | TGCA | 7 cut(s) | 63, 86, 314, 382, 391, 632, 922 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 80, 388, 418 |
| HpyF3I | CTNAG | 7 cut(s) | 34, 77, 117, 195, 224, 415, 492 |
| HpySE526I | ACGT | 2 cut(s) | 384, 939 |
| Hsp92II | CATG | 9 cut(s) | 406, 481, 523, 665, 728, 742, 886, 1040, 1049 |
| Ksp22I | TGATCA | 1 cut(s) | 547 |
| Kzo9I | GATC | 2 cut(s) | 495, 547 |
| LmnI | GCTCC | 2 cut(s) | 706, 970 |
| Lsp1109I | GCAGC | 3 cut(s) | 70, 203, 312 |
| LweI | GCATC | 1 cut(s) | 675 |
| MaeI | CTAG | 2 cut(s) | 684, 998 |
| MaeII | ACGT | 2 cut(s) | 384, 939 |
| MaeIII | GTNAC | 2 cut(s) | 843, 935 |
| MalI | GATC | 2 cut(s) | 497, 549 |
| MboI | GATC | 2 cut(s) | 495, 547 |
| MboII | GAAGA | 5 cut(s) | 94, 347, 593, 829, 905 |
| MflI | RGATCY | 1 cut(s) | 495 |
| MhlI | GDGCHC | 3 cut(s) | 96, 374, 810 |
| MlyI | GAGTC | 1 cut(s) | 740 |
| MnlI | CCTC | 8 cut(s) | 248, 259, 541, 566, 602, 663, 832, 1079 |
| MseI | TTAA | 4 cut(s) | 216, 561, 929, 1084 |
| MslI | CAYNNNNRTG | 3 cut(s) | 68, 660, 729 |
| MspCI | CTTAAG | 2 cut(s) | 215, 560 |
| MspI | CCGG | 1 cut(s) | 855 |
| Mva1269I | GAATGC | 1 cut(s) | 63 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 80, 388, 418 |
| NcoI | CCATGG | 1 cut(s) | 661 |
| NdeII | GATC | 2 cut(s) | 495, 547 |
| NlaIII | CATG | 9 cut(s) | 406, 481, 523, 665, 728, 742, 886, 1040, 1049 |
| NmuCI | GTSAC | 1 cut(s) | 935 |
| NspI | RCATGY | 4 cut(s) | 406, 481, 728, 742 |
| PagI | TCATGA | 1 cut(s) | 519 |
| PciI | ACATGT | 2 cut(s) | 477, 738 |
| PctI | GAATGC | 1 cut(s) | 63 |
| PfeI | GAWTC | 1 cut(s) | 210 |
| PkrI | GCNGC | 3 cut(s) | 85, 193, 327 |
| PleI | GAGTC | 1 cut(s) | 740 |
| PpsI | GAGTC | 1 cut(s) | 740 |
| PscI | ACATGT | 2 cut(s) | 477, 738 |
| Psp124BI | GAGCTC | 1 cut(s) | 96 |
| PspPI | GGNCC | 1 cut(s) | 125 |
| PstNI | CAGNNNCTG | 1 cut(s) | 983 |
| PsuI | RGATCY | 1 cut(s) | 495 |
| RsaI | GTAC | 1 cut(s) | 786 |
| RsaNI | GTAC | 1 cut(s) | 785 |
| RseI | CAYNNNNRTG | 3 cut(s) | 68, 660, 729 |
| SacI | GAGCTC | 1 cut(s) | 96 |
| SaqAI | TTAA | 4 cut(s) | 216, 561, 929, 1084 |
| SatI | GCNGC | 3 cut(s) | 84, 192, 326 |
| Sau3AI | GATC | 2 cut(s) | 495, 547 |
| Sau96I | GGNCC | 1 cut(s) | 125 |
| SchI | GAGTC | 1 cut(s) | 740 |
| SduI | GDGCHC | 3 cut(s) | 96, 374, 810 |
| SfaNI | GCATC | 1 cut(s) | 675 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 68, 660, 729 |
| SmlI | CTYRAG | 6 cut(s) | 215, 407, 422, 560, 696, 1070 |
| SmoI | CTYRAG | 6 cut(s) | 215, 407, 422, 560, 696, 1070 |
| SspI | AATATT | 1 cut(s) | 878 |
| SspMI | CTAG | 2 cut(s) | 684, 998 |
| SstI | GAGCTC | 1 cut(s) | 96 |
| StyI | CCWWGG | 2 cut(s) | 10, 661 |
| TaaI | ACNGT | 3 cut(s) | 812, 833, 979 |
| TaiI | ACGT | 2 cut(s) | 387, 942 |
| TaqI | TCGA | 4 cut(s) | 96, 571, 749, 1062 |
| TaqII | GACCGA | 1 cut(s) | 46 |
| TfiI | GAWTC | 1 cut(s) | 210 |
| Tru1I | TTAA | 4 cut(s) | 216, 561, 929, 1084 |
| Tru9I | TTAA | 4 cut(s) | 216, 561, 929, 1084 |
| TscAI | CASTG | 3 cut(s) | 423, 736, 815 |
| TseFI | GTSAC | 1 cut(s) | 935 |
| TseI | GCWGC | 3 cut(s) | 83, 191, 325 |
| Tsp45I | GTSAC | 1 cut(s) | 935 |
| TspDTI | ATGAA | 5 cut(s) | 348, 486, 492, 779, 871 |
| TspRI | CASTG | 3 cut(s) | 423, 736, 815 |
| Vha464I | CTTAAG | 2 cut(s) | 215, 560 |
| XapI | RAATTY | 4 cut(s) | 52, 199, 449, 463 |
| XceI | RCATGY | 4 cut(s) | 406, 481, 728, 742 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1043 |
| XspI | CTAG | 2 cut(s) | 684, 998 |