Rh5AG114300
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000656
Physical Location & Seq
Forward (+)
10624293 .. 10625154
862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG114300.1

Sequence Viewer

Length: 510 bp
ATGGATGTAGGAGGTGGTGATGGAACTGCGATTGCCAAGATAGTGTCTATGTATCCTCACATCCAGGGAATCAACTTCAATTTGCCAAGTGTTGTATCTAAAGCTCCTAAATTGCAAGGTGTGGAGCATGTGAGTGGAGATATGTTTGAGTTCATACCAAAGACGCAGTCAATCATGTTGATGTTGGTACTCCATAATTGGGATGATGAACACTGTAAGAAGATTTTGAGAAACTGTTGGGAGGCAATACCGGATTATGGGAAGGTAATAGTTGTTGAGCCATTTGTAATGCCTGAACTAGTTGAGGAGACCCCAGAAATGAAGCGCATACTTTACTATGACGTAATCATGATGACCTTGTTTCCTGGTGGTAAGGAGCGAACAACCGCTGAATTGGATATCCTGGCCAAATCCGTAGGTTTCGTTGAAACAAAGATCTTCCCGCTTCCTCGTGGGAGTCATGTTATTGAGTTTCTTAAGGGCGGGAGCGTGGGATTGTTGGATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

169

Amino Acids

18.86

Weight (kDa)

5.52

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 1 - 141 1.2e-38 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 387, 443, 483
AcoI YGGCCR 1 cut(s) 405
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 2 cut(s) 199, 257
AflII CTTAAG 1 cut(s) 476
AgsI TTSAA 2 cut(s) 79, 428
AhlI ACTAGT 1 cut(s) 298
AjnI CCWGG 3 cut(s) 63, 364, 402
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 302
AoxI GGCC 1 cut(s) 405
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AspLEI GCGC 1 cut(s) 327
AsuHPI GGTGA 1 cut(s) 29
BalI TGGCCA 1 cut(s) 407
BauI CACGAG 1 cut(s) 450
BccI CCATC 1 cut(s) 14
BciT130I CCWGG 3 cut(s) 65, 366, 404
BciVI GTATCC 1 cut(s) 63
BcoDI GTCTC 1 cut(s) 302
BcuI ACTAGT 1 cut(s) 298
BfaI CTAG 1 cut(s) 299
BfrI CTTAAG 1 cut(s) 476
BfuI GTATCC 1 cut(s) 63
BglII AGATCT 1 cut(s) 435
Bme1390I CCNGG 3 cut(s) 65, 366, 404
BmrFI CCNGG 3 cut(s) 65, 366, 404
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 64
BsaWI WCCGGW 1 cut(s) 250
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 257
BseBI CCWGG 3 cut(s) 65, 366, 404
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 3 cut(s) 10, 60, 208
BseLI CCNNNNNNNGG 2 cut(s) 199, 257
BseRI GAGGAG 1 cut(s) 320
BshFI GGCC 1 cut(s) 407
BsiSI CCGG 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 199, 257
BsmAI GTCTC 1 cut(s) 302
BsnI GGCC 1 cut(s) 407
Bso31I GGTCTC 1 cut(s) 302
Bsp143I GATC 1 cut(s) 435
BspACI CCGC 3 cut(s) 387, 443, 483
BspANI GGCC 1 cut(s) 407
BspHI TCATGA 1 cut(s) 348
BspTI CTTAAG 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 1 cut(s) 435
BssSI CACGAG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 3 cut(s) 65, 366, 404
Bst4CI ACNGT 2 cut(s) 215, 236
BstAFI CTTAAG 1 cut(s) 476
BstF5I GGATG 3 cut(s) 10, 60, 208
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 1 cut(s) 438
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 1 cut(s) 435
BstNI CCWGG 3 cut(s) 65, 366, 404
BstNSI RCATGY 1 cut(s) 131
BstSCI CCNGG 3 cut(s) 63, 364, 402
BstX2I RGATCY 1 cut(s) 435
BstYI RGATCY 1 cut(s) 435
BsuI GTATCC 1 cut(s) 63
BsuRI GGCC 1 cut(s) 407
BtsCI GGATG 3 cut(s) 10, 60, 208
BtsIMutI CAGTG 1 cut(s) 211
CciI TCATGA 1 cut(s) 348
CfoI GCGC 1 cut(s) 327
CseI GACGC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 188
CviAII CATG 4 cut(s) 128, 175, 349, 461
CviJI RGCY 3 cut(s) 104, 280, 407
CviKI_1 RGCY 3 cut(s) 104, 280, 407
CviQI GTAC 1 cut(s) 188
DpnI GATC 1 cut(s) 437
DpnII GATC 1 cut(s) 435
EaeI YGGCCR 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 302
Eco32I GATATC 1 cut(s) 400
EcoRII CCWGG 3 cut(s) 63, 364, 402
EcoRV GATATC 1 cut(s) 400
FaeI CATG 4 cut(s) 131, 178, 352, 464
FatI CATG 4 cut(s) 127, 174, 348, 460
FauI CCCGC 2 cut(s) 450, 476
FokI GGATG 3 cut(s) 17, 47, 215
FspBI CTAG 1 cut(s) 299
GlaI GCGC 1 cut(s) 326
HaeIII GGCC 1 cut(s) 407
HapII CCGG 1 cut(s) 251
HgaI GACGC 1 cut(s) 172
HhaI GCGC 1 cut(s) 327
Hin1II CATG 4 cut(s) 131, 178, 352, 464
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HinfI GANTC 2 cut(s) 69, 457
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 29
Hpy188III TCNNGA 1 cut(s) 349
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 2 cut(s) 215, 236
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 1 cut(s) 115
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 4 cut(s) 131, 178, 352, 464
HspAI GCGC 1 cut(s) 325
Kzo9I GATC 1 cut(s) 435
LmnI GCTCC 4 cut(s) 109, 124, 376, 486
LpnPI CCDG 9 cut(s) 50, 77, 264, 306, 327, 351, 378, 389, 416
MaeI CTAG 1 cut(s) 299
MaeII ACGT 1 cut(s) 342
MalI GATC 1 cut(s) 437
MboI GATC 1 cut(s) 435
MboII GAAGA 2 cut(s) 232, 430
MflI RGATCY 1 cut(s) 435
MlsI TGGCCA 1 cut(s) 407
MluCI AATT 4 cut(s) 79, 110, 196, 392
MluNI TGGCCA 1 cut(s) 407
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 1 cut(s) 480
MnlI CCTC 5 cut(s) 5, 66, 235, 298, 459
Mox20I TGGCCA 1 cut(s) 407
MscI TGGCCA 1 cut(s) 407
MseI TTAA 1 cut(s) 477
MslI CAYNNNNRTG 2 cut(s) 132, 179
Msp20I TGGCCA 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 476
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 3 cut(s) 65, 366, 404
MvaI CCWGG 3 cut(s) 65, 366, 404
NdeII GATC 1 cut(s) 435
NlaIII CATG 4 cut(s) 131, 178, 352, 464
NspI RCATGY 1 cut(s) 131
PagI TCATGA 1 cut(s) 348
PfeI GAWTC 1 cut(s) 69
PflFI GACNNNGTC 1 cut(s) 166
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
Psp6I CCWGG 3 cut(s) 63, 364, 402
PspGI CCWGG 3 cut(s) 63, 364, 402
PsuI RGATCY 1 cut(s) 435
PsyI GACNNNGTC 1 cut(s) 166
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
RseI CAYNNNNRTG 2 cut(s) 132, 179
SaqAI TTAA 1 cut(s) 477
Sau3AI GATC 1 cut(s) 435
SchI GAGTC 1 cut(s) 466
ScrFI CCNGG 3 cut(s) 65, 366, 404
SetI ASST 7 cut(s) 16, 106, 121, 267, 345, 359, 421
SmiMI CAYNNNNRTG 2 cut(s) 132, 179
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
SpeI ACTAGT 1 cut(s) 298
Sse9I AATT 4 cut(s) 79, 110, 196, 392
SsiI CCGC 3 cut(s) 387, 443, 483
SspMI CTAG 1 cut(s) 299
StyD4I CCNGG 3 cut(s) 63, 364, 402
TaaI ACNGT 2 cut(s) 215, 236
TaiI ACGT 1 cut(s) 345
TasI AATT 4 cut(s) 79, 110, 196, 392
TfiI GAWTC 1 cut(s) 69
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 1 cut(s) 218
TspDTI ATGAA 3 cut(s) 142, 222, 335
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 1 cut(s) 218
Tth111I GACNNNGTC 1 cut(s) 166
Vha464I CTTAAG 1 cut(s) 476
XceI RCATGY 1 cut(s) 131
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.