Rh7BG246000
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000656
Physical Location & Seq
Reverse (-)
21935832 .. 21938737
2906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG246000.1

Sequence Viewer

Length: 1092 bp
ATGGAAAACCAGAGAATTAGCTCAGAAAACTATGTCTCTGCTTGGAATTTGGGAGGTCTAGTTGTCACTCAAATGGCTCTGAAAGCTGCAATTGAGCTCAATGTCTTCAATATCATTGCCAATTCAGGACCCGGAGCTCATCTCACATCGAAAGAAATTGTATCTCAAATTTCAACTGAGAATCCAAATGTGGCAGCTGCGAATTTGAAGCGAATACTAAGAGTTCTAAGTGTTCACTCTCTACTCTCTGTTCAAAGGCCAAGCTCAAGTGGTAAAACAATGCAAGAAGAGACTTATGGTCTAACAAAGGAGACTCTTTGGCTAGTTCCAAATGAAGATGGAGTTTCCTTAGCTCCATTTATTTTGTTATCTTCTGAAATGGACATAGTGAAAAGCTTATCCATGCTCAAAGACACAGTGCTTGAACCTGGGTGCCCTCCTTTCAACAAGGCTTTTGGTATGTCCATATATGAGTACATGTCTGAGAAACCTGACCTGAGCCAATTGTTTAATAAGGGTGTAGGCCAAAGTTCCATCCTCGATTTTGAAGAGGTGTTAAAGGTTTACAAAGGTTTTGAAGAGGTAGAAGAGTTGATGGATGTTGGAGGAGGTGATGGAATGACAATTTCAAAAGTAGTATCTAGATATCCACACATTCATGGGATTAACTTTGATTTGCCTAACGTTATCGCTCAAGGAACTGCTAAATATCAAACTGCAGGTGTGAAATATGTTGGTGGAGATGTGTTTCAGTTTATACCAAATGCACAGTCCATTATGTTGAAGTGGGTACTTCATAATTGGAATGATGATCTGTGTAAGAAAATATTGAAACGTTGTTGGGAGGCATTACCAAAAACCGGGAAGGTAATAGTTGTGGAATTTGCAATACCTGAAATACTAGAGAAAACCGAAGAGGTAAAGAAAATTGTGGCATTAGACGTGGTCATGATGGCTACTCCCGGTGGTAGAGAACGAACAATTGCTGAGTTTGACGACCTATCAAAAGCTGCAGGCTTTGTTGAAACAAAGATTTTCTCAATTTCAAATGGGTGTTATGTTCTGGAGTTTCACAAAGTTGAAACATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

363

Amino Acids

40.15

Weight (kDa)

5.34

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 21 - 107 1.8e-11 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 135 - 340 1.3e-55 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 710
Acc36I ACCTGC 1 cut(s) 710
AccB1I GGYRCC 1 cut(s) 432
AclI AACGTT 2 cut(s) 684, 835
AcsI RAATTY 4 cut(s) 46, 168, 202, 881
AfaI GTAC 2 cut(s) 476, 792
AfiI CCNNNNNNNGG 1 cut(s) 860
AflIII ACRYGT 1 cut(s) 477
AhdI GACNNNNNGTC 1 cut(s) 297
AjiI CACGTC 1 cut(s) 943
AjnI CCWGG 1 cut(s) 427
AluBI AGCT 9 cut(s) 21, 86, 97, 137, 197, 264, 353, 396, 1010
AluI AGCT 9 cut(s) 21, 86, 97, 137, 197, 264, 353, 396, 1010
Alw21I GWGCWC 2 cut(s) 99, 139
Alw26I GTCTC 3 cut(s) 40, 284, 305
AoxI GGCC 2 cut(s) 257, 523
ApeKI GCWGC 4 cut(s) 86, 194, 197, 1010
ApoI RAATTY 4 cut(s) 46, 168, 202, 881
ArsI GACNNNNNNTTYG 2 cut(s) 755, 787
AspS9I GGNCC 1 cut(s) 128
AsuC2I CCSGG 3 cut(s) 132, 862, 963
AsuHPI GGTGA 1 cut(s) 623
AvaII GGWCC 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 437
BanI GGYRCC 1 cut(s) 432
BanII GRGCYC 2 cut(s) 99, 139
BbsI GAAGAC 1 cut(s) 97
Bbv12I GWGCWC 2 cut(s) 99, 139
BbvI GCAGC 4 cut(s) 73, 184, 206, 997
BccI CCATC 5 cut(s) 332, 542, 589, 608, 946
BciT130I CCWGG 1 cut(s) 429
BcnI CCSGG 3 cut(s) 132, 862, 963
BcoDI GTCTC 3 cut(s) 40, 284, 305
BfaI CTAG 5 cut(s) 59, 323, 642, 902, 1090
BfmI CTRYAG 2 cut(s) 717, 1011
BfuAI ACCTGC 1 cut(s) 710
BisI GCNGC 4 cut(s) 87, 195, 198, 1011
BlsI GCNGC 4 cut(s) 88, 196, 199, 1012
Bme1390I CCNGG 4 cut(s) 132, 429, 862, 963
Bme18I GGWCC 1 cut(s) 128
BmeRI GACNNNNNGTC 1 cut(s) 297
BmgBI CACGTC 1 cut(s) 943
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 2 cut(s) 130, 434
BmrFI CCNGG 4 cut(s) 132, 429, 862, 963
BpiI GAAGAC 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 126, 158
BpmI CTGGAG 1 cut(s) 1085
Bpu10I CCTNAGC 2 cut(s) 349, 497
BpuEI CTTGAG 2 cut(s) 250, 678
BpuMI CCSGG 3 cut(s) 132, 862, 963
BsaJI CCNNGG 1 cut(s) 428
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 1 cut(s) 860
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 428
BseGI GGATG 2 cut(s) 534, 604
BseLI CCNNNNNNNGG 1 cut(s) 860
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 5 cut(s) 36, 168, 474, 488, 978
BseRI GAGGAG 1 cut(s) 621
BseSI GKGCMC 1 cut(s) 437
BseXI GCAGC 4 cut(s) 73, 184, 206, 997
BshFI GGCC 2 cut(s) 259, 525
BshNI GGYRCC 1 cut(s) 432
BsiHKAI GWGCWC 2 cut(s) 99, 139
BsiSI CCGG 3 cut(s) 132, 861, 963
BslI CCNNNNNNNGG 1 cut(s) 860
BsmAI GTCTC 3 cut(s) 40, 284, 305
BsnI GGCC 2 cut(s) 259, 525
Bsp1286I GDGCHC 3 cut(s) 99, 139, 437
Bsp143I GATC 1 cut(s) 811
BspANI GGCC 2 cut(s) 259, 525
BspCNI CTCAG 5 cut(s) 35, 169, 475, 489, 979
BspHI TCATGA 1 cut(s) 948
BspLI GGNNCC 2 cut(s) 130, 434
BspMAI CTGCAG 2 cut(s) 721, 1015
BspMI ACCTGC 1 cut(s) 710
BspT107I GGYRCC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 428
BssMI GATC 1 cut(s) 811
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 418, 771
Bst6I CTCTTC 5 cut(s) 282, 543, 573, 582, 909
BstC8I GCNNGC 1 cut(s) 1015
BstDEI CTNAG 8 cut(s) 22, 177, 218, 227, 349, 483, 497, 987
BstF5I GGATG 2 cut(s) 534, 604
BstKTI GATC 1 cut(s) 814
BstMAI GTCTC 3 cut(s) 40, 284, 305
BstMBI GATC 1 cut(s) 811
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 429
BstNSI RCATGY 1 cut(s) 481
BstSCI CCNGG 4 cut(s) 130, 427, 860, 961
BstSFI CTRYAG 2 cut(s) 717, 1011
BstSLI GKGCMC 1 cut(s) 437
BstV1I GCAGC 4 cut(s) 73, 184, 206, 997
BstV2I GAAGAC 1 cut(s) 97
BsuRI GGCC 2 cut(s) 259, 525
BtrI CACGTC 1 cut(s) 943
BtsCI GGATG 2 cut(s) 534, 604
BtsIMutI CAGTG 1 cut(s) 423
BveI ACCTGC 1 cut(s) 710
Cac8I GCNNGC 1 cut(s) 1015
CciI TCATGA 1 cut(s) 948
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 2 cut(s) 475, 791
CviAII CATG 4 cut(s) 403, 478, 659, 949
CviQI GTAC 2 cut(s) 475, 791
DdeI CTNAG 8 cut(s) 22, 177, 218, 227, 349, 483, 497, 987
DpnI GATC 1 cut(s) 813
DpnII GATC 1 cut(s) 811
DriI GACNNNNNGTC 1 cut(s) 297
Eam1104I CTCTTC 5 cut(s) 282, 543, 573, 582, 909
Eam1105I GACNNNNNGTC 1 cut(s) 297
EarI CTCTTC 5 cut(s) 282, 543, 573, 582, 909
Ecl136II GAGCTC 2 cut(s) 97, 137
Eco24I GRGCYC 2 cut(s) 99, 139
Eco32I GATATC 1 cut(s) 647
Eco47I GGWCC 1 cut(s) 128
Eco53kI GAGCTC 2 cut(s) 97, 137
EcoICRI GAGCTC 2 cut(s) 97, 137
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 427
EcoRV GATATC 1 cut(s) 647
EcoT38I GRGCYC 2 cut(s) 99, 139
FaeI CATG 4 cut(s) 406, 481, 662, 952
FatI CATG 4 cut(s) 402, 477, 658, 948
Fnu4HI GCNGC 4 cut(s) 87, 195, 198, 1011
FokI GGATG 2 cut(s) 521, 611
FriOI GRGCYC 2 cut(s) 99, 139
Fsp4HI GCNGC 4 cut(s) 87, 195, 198, 1011
FspBI CTAG 5 cut(s) 59, 323, 642, 902, 1090
GluI GCNGC 4 cut(s) 87, 195, 198, 1011
GsuI CTGGAG 1 cut(s) 1085
HaeIII GGCC 2 cut(s) 259, 525
HapII CCGG 3 cut(s) 132, 861, 963
Hin1II CATG 4 cut(s) 406, 481, 662, 952
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 2 cut(s) 181, 313
HpaII CCGG 3 cut(s) 132, 861, 963
HphI GGTGA 1 cut(s) 623
Hpy166II GTNNAC 2 cut(s) 235, 565
Hpy188I TCNGA 4 cut(s) 25, 81, 376, 484
Hpy188III TCNNGA 4 cut(s) 126, 642, 949, 1064
Hpy8I GTNNAC 2 cut(s) 235, 565
HpyAV CCTTC 1 cut(s) 859
HpyCH4III ACNGT 2 cut(s) 418, 771
HpyCH4IV ACGT 3 cut(s) 684, 835, 942
HpyCH4V TGCA 6 cut(s) 89, 283, 719, 767, 887, 1013
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 8 cut(s) 22, 177, 218, 227, 349, 483, 497, 987
HpySE526I ACGT 3 cut(s) 684, 835, 942
Hsp92II CATG 4 cut(s) 406, 481, 662, 952
Kzo9I GATC 1 cut(s) 811
LmnI GCTCC 2 cut(s) 134, 358
Lsp1109I GCAGC 4 cut(s) 73, 184, 206, 997
MaeI CTAG 5 cut(s) 59, 323, 642, 902, 1090
MaeII ACGT 3 cut(s) 684, 835, 942
MaeIII GTNAC 1 cut(s) 64
MalI GATC 1 cut(s) 813
MboI GATC 1 cut(s) 811
MboII GAAGA 8 cut(s) 97, 299, 347, 363, 560, 590, 599, 926
MfeI CAATTG 3 cut(s) 90, 503, 981
MhlI GDGCHC 3 cut(s) 99, 139, 437
MlyI GAGTC 1 cut(s) 307
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 9 cut(s) 47, 447, 544, 548, 574, 599, 602, 838, 910
MseI TTAA 3 cut(s) 510, 557, 666
MslI CAYNNNNRTG 2 cut(s) 71, 657
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 3 cut(s) 132, 861, 963
MspR9I CCNGG 4 cut(s) 132, 429, 862, 963
MunI CAATTG 3 cut(s) 90, 503, 981
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 1 cut(s) 83
NciI CCSGG 3 cut(s) 132, 862, 963
NdeII GATC 1 cut(s) 811
NlaIII CATG 4 cut(s) 406, 481, 662, 952
NlaIV GGNNCC 2 cut(s) 130, 434
NmuCI GTSAC 1 cut(s) 64
NspI RCATGY 1 cut(s) 481
PagI TCATGA 1 cut(s) 948
PaqCI CACCTGC 1 cut(s) 710
PciI ACATGT 1 cut(s) 477
PfeI GAWTC 1 cut(s) 181
PflFI GACNNNGTC 1 cut(s) 944
PkrI GCNGC 4 cut(s) 88, 196, 199, 1012
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 128
PscI ACATGT 1 cut(s) 477
Psp124BI GAGCTC 2 cut(s) 99, 139
Psp1406I AACGTT 2 cut(s) 684, 835
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 2 cut(s) 130, 434
PspPI GGNCC 1 cut(s) 128
PspPPI RGGWCCY 1 cut(s) 128
PstI CTGCAG 2 cut(s) 721, 1015
PsyI GACNNNGTC 1 cut(s) 944
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 2 cut(s) 476, 792
RsaNI GTAC 2 cut(s) 475, 791
RseI CAYNNNNRTG 2 cut(s) 71, 657
SacI GAGCTC 2 cut(s) 99, 139
SaqAI TTAA 3 cut(s) 510, 557, 666
SatI GCNGC 4 cut(s) 87, 195, 198, 1011
Sau3AI GATC 1 cut(s) 811
Sau96I GGNCC 1 cut(s) 128
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 4 cut(s) 132, 429, 862, 963
SduI GDGCHC 3 cut(s) 99, 139, 437
SfcI CTRYAG 2 cut(s) 717, 1011
SinI GGWCC 1 cut(s) 128
SmiMI CAYNNNNRTG 2 cut(s) 71, 657
SmlI CTYRAG 2 cut(s) 265, 693
SmoI CTYRAG 2 cut(s) 265, 693
SspI AATATT 1 cut(s) 828
SspMI CTAG 5 cut(s) 59, 323, 642, 902, 1090
SstI GAGCTC 2 cut(s) 99, 139
StyD4I CCNGG 4 cut(s) 130, 427, 860, 961
TaaI ACNGT 2 cut(s) 418, 771
TaiI ACGT 3 cut(s) 687, 838, 945
TaqI TCGA 2 cut(s) 149, 540
TatI WGTACW 1 cut(s) 474
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 3 cut(s) 510, 557, 666
Tru9I TTAA 3 cut(s) 510, 557, 666
TscAI CASTG 1 cut(s) 423
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 4 cut(s) 86, 194, 197, 1010
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 3 cut(s) 348, 647, 785
TspRI CASTG 1 cut(s) 423
Tth111I GACNNNGTC 1 cut(s) 944
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 4 cut(s) 46, 168, 202, 881
XbaI TCTAGA 1 cut(s) 641
XceI RCATGY 1 cut(s) 481
XspI CTAG 5 cut(s) 59, 323, 642, 902, 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.