Rroxscaffold_1G00035380
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000656
Physical Location & Seq
Forward (+)
51486573 .. 51492948
6376 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035380.1

Sequence Viewer

Length: 555 bp
ATGGCTCTGAAGTCTGAAATGCATTTGATGCAGAGATTGTACGTGCTTAAACACATGATGCTTGAACCAGAGTGTTCTCCTTTCGACAAAGCCCACGGACAGACCTTGTATGAATATATGTCCGAGAAGCCTGAGCTTATTCAATTGTTTGATAAGGTAATGAGAATTACCAGTGGCGGATCTAGGATTCAAAATTGGGGTGGCTTTGAAAAGGTGCTTGAGGTTTATAGAGGCTTCAATGAGTTAAAAGAGCTAATGGATGTTGGGGGTGGCGATGGAAGTTCGATTGCAAAAATAGTGTCTATGCATCTTCATATCCATGGCATCAACTTCAATTTAGAGAAGAACAACAATACTTCTACAATAGTTGATATAGAAATGGCTTCTTCTGTTGCAGCGACTATCGTTGAACAAGGCGGCGATGCCTCCATCTTTGCCCTCCACTGTGACATAATCACATCCCACATCCTCACCCGCCTCGACTGTCCCACCCTTGCCTCCGTCGCATGTGCTTCCTCACAATTGCAAGCCTTCTCCGCCGAACAGACACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00950 ko01110 map00950 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

184

Amino Acids

20.37

Weight (kDa)

5.41

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 20 - 115 6.5e-10 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 177, 417, 475, 537
AclWI GGATC 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 29
AfaI GTAC 1 cut(s) 41
AgsI TTSAA 7 cut(s) 65, 143, 191, 209, 238, 334, 410
AluBI AGCT 2 cut(s) 136, 253
AluI AGCT 2 cut(s) 136, 253
AlwI GGATC 1 cut(s) 187
ApeKI GCWGC 1 cut(s) 395
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AsuHPI GGTGA 1 cut(s) 463
BbvI GCAGC 1 cut(s) 407
BccI CCATC 2 cut(s) 269, 437
BfaI CTAG 2 cut(s) 183, 553
BisI GCNGC 2 cut(s) 396, 418
BlsI GCNGC 2 cut(s) 397, 419
BmsI GCATC 5 cut(s) 18, 48, 316, 333, 412
Bpu10I CCTNAGC 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 239
BsaAI YACGTR 1 cut(s) 43
BsaJI CCNNGG 2 cut(s) 94, 319
Bse1I ACTGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 94, 319
BseGI GGATG 3 cut(s) 265, 458, 465
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 171
BseXI GCAGC 1 cut(s) 407
BslFI GGGAC 1 cut(s) 471
BsmFI GGGAC 1 cut(s) 471
Bsp143I GATC 1 cut(s) 179
Bsp19I CCATGG 1 cut(s) 319
BspACI CCGC 4 cut(s) 177, 417, 475, 537
BspCNI CTCAG 1 cut(s) 124
BspPI GGATC 1 cut(s) 187
BsrI ACTGG 1 cut(s) 171
BssECI CCNNGG 2 cut(s) 94, 319
BssMI GATC 1 cut(s) 179
BssT1I CCWWGG 1 cut(s) 319
Bst4CI ACNGT 2 cut(s) 446, 485
BstAPI GCANNNNNTGC 1 cut(s) 28
BstBAI YACGTR 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 1 cut(s) 132
BstDSI CCRYGG 2 cut(s) 94, 319
BstF5I GGATG 3 cut(s) 265, 458, 465
BstKTI GATC 1 cut(s) 182
BstMBI GATC 1 cut(s) 179
BstMWI GCNNNNNNNGC 3 cut(s) 28, 503, 536
BstNSI RCATGY 1 cut(s) 510
BstV1I GCAGC 1 cut(s) 407
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BtgI CCRYGG 2 cut(s) 94, 319
BtgZI GCGATG 2 cut(s) 288, 435
BtsCI GGATG 3 cut(s) 265, 458, 465
BtsIMutI CAGTG 2 cut(s) 178, 442
Cac8I GCNNGC 1 cut(s) 528
Csp6I GTAC 1 cut(s) 40
CviAII CATG 3 cut(s) 55, 320, 507
CviJI RGCY 9 cut(s) 5, 92, 130, 136, 204, 234, 253, 383, 530
CviKI_1 RGCY 9 cut(s) 5, 92, 130, 136, 204, 234, 253, 383, 530
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 1 cut(s) 132
DpnI GATC 1 cut(s) 181
DpnII GATC 1 cut(s) 179
EciI GGCGGA 2 cut(s) 192, 526
Eco130I CCWWGG 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 319
EcoT22I ATGCAT 2 cut(s) 24, 309
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 3 cut(s) 58, 323, 510
FaqI GGGAC 1 cut(s) 471
FatI CATG 3 cut(s) 54, 319, 506
FauI CCCGC 1 cut(s) 482
Fnu4HI GCNGC 2 cut(s) 396, 418
FokI GGATG 3 cut(s) 272, 445, 452
Fsp4HI GCNGC 2 cut(s) 396, 418
FspBI CTAG 2 cut(s) 183, 553
GluI GCNGC 2 cut(s) 396, 418
Hin1II CATG 3 cut(s) 58, 323, 510
HinfI GANTC 1 cut(s) 187
HphI GGTGA 1 cut(s) 463
Hpy188I TCNGA 3 cut(s) 9, 16, 124
Hpy99I CGWCG 1 cut(s) 506
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 2 cut(s) 446, 485
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 6 cut(s) 22, 31, 290, 307, 395, 526
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 503, 536
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 3 cut(s) 58, 323, 510
Kzo9I GATC 1 cut(s) 179
LpnPI CCDG 3 cut(s) 81, 144, 184
Lsp1109I GCAGC 1 cut(s) 407
LweI GCATC 5 cut(s) 18, 48, 316, 333, 412
MaeI CTAG 2 cut(s) 183, 553
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 446
MalI GATC 1 cut(s) 181
MboI GATC 1 cut(s) 179
MboII GAAGA 3 cut(s) 302, 355, 378
MfeI CAATTG 2 cut(s) 143, 521
MflI RGATCY 1 cut(s) 179
MluCI AATT 5 cut(s) 143, 165, 193, 334, 521
MnlI CCTC 8 cut(s) 214, 224, 436, 449, 479, 488, 508, 526
Mph1103I ATGCAT 2 cut(s) 24, 309
MseI TTAA 2 cut(s) 48, 245
MslI CAYNNNNRTG 1 cut(s) 318
MunI CAATTG 2 cut(s) 143, 521
MwoI GCNNNNNNNGC 3 cut(s) 28, 503, 536
NcoI CCATGG 1 cut(s) 319
NdeII GATC 1 cut(s) 179
NlaIII CATG 3 cut(s) 58, 323, 510
NmuCI GTSAC 1 cut(s) 446
NsiI ATGCAT 2 cut(s) 24, 309
NspI RCATGY 1 cut(s) 510
PfeI GAWTC 1 cut(s) 187
PkrI GCNGC 2 cut(s) 397, 419
Ppu21I YACGTR 1 cut(s) 43
PsuI RGATCY 1 cut(s) 179
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 318
SaqAI TTAA 2 cut(s) 48, 245
SatI GCNGC 2 cut(s) 396, 418
Sau3AI GATC 1 cut(s) 179
SetI ASST 7 cut(s) 45, 107, 138, 159, 216, 225, 255
SfaNI GCATC 5 cut(s) 18, 48, 316, 333, 412
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 5 cut(s) 143, 165, 193, 334, 521
SsiI CCGC 4 cut(s) 177, 417, 475, 537
SspMI CTAG 2 cut(s) 183, 553
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 446, 485
TaiI ACGT 1 cut(s) 45
TaqI TCGA 3 cut(s) 84, 284, 480
TasI AATT 5 cut(s) 143, 165, 193, 334, 521
TauI GCSGC 1 cut(s) 420
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 2 cut(s) 48, 245
Tru9I TTAA 2 cut(s) 48, 245
TscAI CASTG 2 cut(s) 178, 449
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 2 cut(s) 126, 302
TspGWI ACGGA 2 cut(s) 111, 490
TspRI CASTG 2 cut(s) 178, 449
XceI RCATGY 1 cut(s) 510
XspI CTAG 2 cut(s) 183, 553
Zsp2I ATGCAT 2 cut(s) 24, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.