Rh5DG110600
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000656
Physical Location & Seq
Forward (+)
10098331 .. 10099456
1126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG110600.1

Sequence Viewer

Length: 729 bp
ATGCTCGTTGCGAAGATCTTTTACATGCTCAAGTACAAGGTGCTTGAACCGGAGAGTTCTCCCTTTCTCAAAGCCCATGGAGAGAGTCTTTATGACTACATGTCTAACAAGCCTGAGCTGTACCAATTGTTTGACAAGGTGATGGGACTAAATTCTTACCTATATTTTGATCATAAGGTGTTTGAGGTTTACAAAGGTTTCGAAGAGGTGAAGGAGCTGATGGATGTAGGAGGTGGTGATGGAACTGCGATTGCCAAGATAGTGTCTATGTATCCTCACATCCATGGAATCAACTTCAATTTGCCAAGTGTTATATCTAAAGCTCCTAACTTGCAAGGTGTGGAGCATGTAAGTGGAGATATGTTTGATTTAATACCAAAGACGCAGTCAGTCATGTTGATGTGGGTACTCCATAATTGGGATGATGAACACTGTAAGAAGATTTTGAGAAACTGTTGGGAGGTAATACCGGATTATGGAAAGGTAATAGTTGTTGAGCCATTTGTAATGCCTGAACTAGTTGAGGAGACCCCAGAAATGAAGCGCATACTTTACTATGACGTAATCATGATGACCTTGTTTCCTGGTGGTAAGGAGCGAACAACCGCTGAATTGGACATCCTGGCCAAATCCGTAGGTTTCGTTGAAACAAAGATCTTCCCGCTTCCTCGTGGGAGTCATGTTATTGAGTTTCTTAAGGGCGGGAGCGTGGGATTGTTGGATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

242

Amino Acids

27.66

Weight (kDa)

5.51

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 7 - 214 2.7e-48 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 606, 662, 702
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 1 cut(s) 151
AfaI GTAC 3 cut(s) 35, 122, 408
AfiI CCNNNNNNNGG 2 cut(s) 418, 476
AflII CTTAAG 1 cut(s) 695
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 3 cut(s) 47, 298, 647
AhdI GACNNNNNGTC 1 cut(s) 100
AhlI ACTAGT 1 cut(s) 517
AjnI CCWGG 2 cut(s) 583, 621
AluBI AGCT 3 cut(s) 118, 217, 323
AluI AGCT 3 cut(s) 118, 217, 323
Alw26I GTCTC 1 cut(s) 521
AoxI GGCC 1 cut(s) 624
ApoI RAATTY 1 cut(s) 151
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
AspLEI GCGC 1 cut(s) 546
AsuHPI GGTGA 3 cut(s) 151, 220, 248
AsuII TTCGAA 1 cut(s) 201
BalI TGGCCA 1 cut(s) 626
BarI GAAGNNNNNNTAC 1 cut(s) 37
BauI CACGAG 1 cut(s) 669
BccI CCATC 3 cut(s) 136, 214, 233
BciT130I CCWGG 2 cut(s) 585, 623
BciVI GTATCC 1 cut(s) 282
BclI TGATCA 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 521
BcuI ACTAGT 1 cut(s) 517
BfaI CTAG 1 cut(s) 518
BfrI CTTAAG 1 cut(s) 695
BfuI GTATCC 1 cut(s) 282
BglII AGATCT 2 cut(s) 15, 654
Bme1390I CCNGG 2 cut(s) 585, 623
BmeRI GACNNNNNGTC 1 cut(s) 100
BmrFI CCNGG 2 cut(s) 585, 623
Bpu10I CCTNAGC 1 cut(s) 114
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 521
BsaJI CCNNGG 2 cut(s) 76, 283
BsaWI WCCGGW 2 cut(s) 49, 469
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 476
BseBI CCWGG 2 cut(s) 585, 623
BseDI CCNNGG 2 cut(s) 76, 283
BseGI GGATG 4 cut(s) 229, 279, 427, 618
BseLI CCNNNNNNNGG 2 cut(s) 418, 476
BseMII CTCAG 1 cut(s) 105
BseRI GAGGAG 1 cut(s) 539
BshFI GGCC 1 cut(s) 626
BsiSI CCGG 2 cut(s) 50, 470
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 418, 476
BsmAI GTCTC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 1 cut(s) 626
Bso31I GGTCTC 1 cut(s) 521
Bsp119I TTCGAA 1 cut(s) 201
Bsp143I GATC 3 cut(s) 15, 169, 654
Bsp19I CCATGG 2 cut(s) 76, 283
BspACI CCGC 3 cut(s) 606, 662, 702
BspANI GGCC 1 cut(s) 626
BspCNI CTCAG 1 cut(s) 106
BspHI TCATGA 1 cut(s) 567
BspT104I TTCGAA 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 695
BspTNI GGTCTC 1 cut(s) 521
BssECI CCNNGG 2 cut(s) 76, 283
BssMI GATC 3 cut(s) 15, 169, 654
BssSI CACGAG 1 cut(s) 669
BssT1I CCWWGG 2 cut(s) 76, 283
Bst2BI CACGAG 1 cut(s) 669
Bst2UI CCWGG 2 cut(s) 585, 623
Bst4CI ACNGT 2 cut(s) 434, 455
Bst6I CTCTTC 1 cut(s) 198
BstAFI CTTAAG 1 cut(s) 695
BstBI TTCGAA 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 114
BstDSI CCRYGG 2 cut(s) 76, 283
BstF5I GGATG 4 cut(s) 229, 279, 427, 618
BstHHI GCGC 1 cut(s) 546
BstKTI GATC 3 cut(s) 18, 172, 657
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 3 cut(s) 15, 169, 654
BstNI CCWGG 2 cut(s) 585, 623
BstNSI RCATGY 3 cut(s) 28, 103, 350
BstSCI CCNGG 2 cut(s) 583, 621
BstX2I RGATCY 2 cut(s) 15, 654
BstYI RGATCY 2 cut(s) 15, 654
BsuI GTATCC 1 cut(s) 282
BsuRI GGCC 1 cut(s) 626
BtgI CCRYGG 2 cut(s) 76, 283
BtsCI GGATG 4 cut(s) 229, 279, 427, 618
BtsIMutI CAGTG 1 cut(s) 430
CciI TCATGA 1 cut(s) 567
CfoI GCGC 1 cut(s) 546
CseI GACGC 1 cut(s) 391
Csp6I GTAC 3 cut(s) 34, 121, 407
CviAII CATG 8 cut(s) 25, 77, 100, 284, 347, 394, 568, 680
CviJI RGCY 7 cut(s) 74, 112, 118, 217, 323, 499, 626
CviKI_1 RGCY 7 cut(s) 74, 112, 118, 217, 323, 499, 626
CviQI GTAC 3 cut(s) 34, 121, 407
DdeI CTNAG 1 cut(s) 114
DpnI GATC 3 cut(s) 17, 171, 656
DpnII GATC 3 cut(s) 15, 169, 654
DriI GACNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 624
Eam1104I CTCTTC 1 cut(s) 198
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 2 cut(s) 76, 283
Eco31I GGTCTC 1 cut(s) 521
EcoRII CCWGG 2 cut(s) 583, 621
EcoT14I CCWWGG 2 cut(s) 76, 283
ErhI CCWWGG 2 cut(s) 76, 283
FaeI CATG 8 cut(s) 28, 80, 103, 287, 350, 397, 571, 683
FaqI GGGAC 1 cut(s) 159
FatI CATG 8 cut(s) 24, 76, 99, 283, 346, 393, 567, 679
FauI CCCGC 2 cut(s) 669, 695
FbaI TGATCA 1 cut(s) 169
FokI GGATG 4 cut(s) 236, 266, 434, 605
FspBI CTAG 1 cut(s) 518
GlaI GCGC 1 cut(s) 545
HaeIII GGCC 1 cut(s) 626
HapII CCGG 2 cut(s) 50, 470
HgaI GACGC 1 cut(s) 391
HhaI GCGC 1 cut(s) 546
Hin1II CATG 8 cut(s) 28, 80, 103, 287, 350, 397, 571, 683
Hin6I GCGC 1 cut(s) 544
HinP1I GCGC 1 cut(s) 544
HinfI GANTC 3 cut(s) 85, 288, 676
HpaII CCGG 2 cut(s) 50, 470
HphI GGTGA 3 cut(s) 151, 220, 248
Hpy166II GTNNAC 1 cut(s) 190
Hpy188III TCNNGA 1 cut(s) 568
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 2 cut(s) 434, 455
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 1 cut(s) 334
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 561
Hsp92II CATG 8 cut(s) 28, 80, 103, 287, 350, 397, 571, 683
HspAI GCGC 1 cut(s) 544
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 3 cut(s) 15, 169, 654
LmnI GCTCC 5 cut(s) 214, 328, 343, 595, 705
LpnPI CCDG 9 cut(s) 63, 126, 483, 525, 546, 570, 597, 608, 635
MaeI CTAG 1 cut(s) 518
MaeII ACGT 1 cut(s) 561
MalI GATC 3 cut(s) 17, 171, 656
MboI GATC 3 cut(s) 15, 169, 654
MboII GAAGA 4 cut(s) 25, 215, 451, 649
MfeI CAATTG 1 cut(s) 125
MflI RGATCY 2 cut(s) 15, 654
MlsI TGGCCA 1 cut(s) 626
MluCI AATT 5 cut(s) 125, 151, 298, 415, 611
MluNI TGGCCA 1 cut(s) 626
MlyI GAGTC 2 cut(s) 94, 685
MmeI TCCRAC 1 cut(s) 699
MnlI CCTC 7 cut(s) 178, 199, 224, 285, 454, 517, 678
Mox20I TGGCCA 1 cut(s) 626
MscI TGGCCA 1 cut(s) 626
MseI TTAA 2 cut(s) 371, 696
MslI CAYNNNNRTG 3 cut(s) 282, 351, 398
Msp20I TGGCCA 1 cut(s) 626
MspA1I CMGCKG 1 cut(s) 608
MspCI CTTAAG 1 cut(s) 695
MspI CCGG 2 cut(s) 50, 470
MspR9I CCNGG 2 cut(s) 585, 623
MunI CAATTG 1 cut(s) 125
MvaI CCWGG 2 cut(s) 585, 623
NcoI CCATGG 2 cut(s) 76, 283
NdeII GATC 3 cut(s) 15, 169, 654
NlaIII CATG 8 cut(s) 28, 80, 103, 287, 350, 397, 571, 683
NspI RCATGY 3 cut(s) 28, 103, 350
NspV TTCGAA 1 cut(s) 201
PagI TCATGA 1 cut(s) 567
PciI ACATGT 1 cut(s) 99
PfeI GAWTC 1 cut(s) 288
PflFI GACNNNGTC 1 cut(s) 385
PleI GAGTC 2 cut(s) 93, 684
PpsI GAGTC 2 cut(s) 93, 684
PscI ACATGT 1 cut(s) 99
Psp6I CCWGG 2 cut(s) 583, 621
PspGI CCWGG 2 cut(s) 583, 621
PsuI RGATCY 2 cut(s) 15, 654
PsyI GACNNNGTC 1 cut(s) 385
RsaI GTAC 3 cut(s) 35, 122, 408
RsaNI GTAC 3 cut(s) 34, 121, 407
RseI CAYNNNNRTG 3 cut(s) 282, 351, 398
SaqAI TTAA 2 cut(s) 371, 696
Sau3AI GATC 3 cut(s) 15, 169, 654
SchI GAGTC 2 cut(s) 94, 685
ScrFI CCNGG 2 cut(s) 585, 623
SfuI TTCGAA 1 cut(s) 201
SmiMI CAYNNNNRTG 3 cut(s) 282, 351, 398
SmlI CTYRAG 2 cut(s) 29, 695
SmoI CTYRAG 2 cut(s) 29, 695
SpeI ACTAGT 1 cut(s) 517
Sse9I AATT 5 cut(s) 125, 151, 298, 415, 611
SsiI CCGC 3 cut(s) 606, 662, 702
SspMI CTAG 1 cut(s) 518
StyD4I CCNGG 2 cut(s) 583, 621
StyI CCWWGG 2 cut(s) 76, 283
TaaI ACNGT 2 cut(s) 434, 455
TaiI ACGT 1 cut(s) 564
TaqI TCGA 1 cut(s) 201
TasI AATT 5 cut(s) 125, 151, 298, 415, 611
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 2 cut(s) 371, 696
Tru9I TTAA 2 cut(s) 371, 696
TscAI CASTG 1 cut(s) 437
TspDTI ATGAA 2 cut(s) 441, 554
TspGWI ACGGA 1 cut(s) 622
TspRI CASTG 1 cut(s) 437
Tth111I GACNNNGTC 1 cut(s) 385
Vha464I CTTAAG 1 cut(s) 695
XapI RAATTY 1 cut(s) 151
XceI RCATGY 3 cut(s) 28, 103, 350
XspI CTAG 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.