Rroxscaffold_3G00247690
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000656
Physical Location & Seq
Forward (+)
41089304 .. 41096081
6778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247690.1

Sequence Viewer

Length: 1089 bp
ATGGAAAACCAGAGAATTAGCTCAAAAAACTATGTCTCTGCTTGGAATTTGGGGGCTCTAGTTGTCACTGAAATGGCTCTGAAAGCTGCAATCGAGCTCAATGTCTTCAATATCATTGCCAATTCAGTACCCGGAGCTCATCTCACATCGAAAGAAATTGTATCTCAAATCTCAACTAAGAATCCAAATGTAGCAGCTGCGAATTTGAAGCGAATACTAAGAGTCCTAAGTGTTCACTCTCTACTCTCTGTACAAAGGCCAAGTTCAAATGGTAAAACAATGCAAGAAGAGACTTATGGTCTAACAAAGGAGACTCTTTGCCTAGTTCCAAATGAAGATGGAGTTTCCTTAGCTCCATTTATTTTGTTATATTCTGAAATGTACATGGTGAAAAGCTTATCCATGCTCAAAGACACAGTACTTGAACCTGGGTGCCTTCCTTTCAACAAGGCTTATGGTGTGTCCATATATGAGTACATGTCTGAGAAACCTGACCTGAGCCAATTGTTTAATAAAGCTATTGGCCAAAGTACGATCCTCGATTTTGAAGAGGTGTTAAAGGTTTACAAAGGTTTTGAAGAGCTAGAAGAGTTGATGGATGTTGGAGGAGGTGATGGAACGACAATTTCAAAAGTAGTTTCTAGATATCCACACATTCATGGGATTAACTTCGATTTGCCTAACGTTATCGCTCAAGGAACTGCTAGATATCAAACAGGTGTGAAATATGTTGGTGGAGATATGTTTCAGTTTATACCAAATGCACAGTCGATTATGTTGAAGTGGGTACTTCATAACTGGGACGATGATCTATGTAAGAAAATATTGAAGCGCTGTTGGGAGGCATTACCAAAAACTGGAAAGGTAATAGTTGTGGAACTTGCAATACCTGAAATACTAGAGAAAACCGAAGAGTTAAAGAAAATAGTGGCATTAGACATGGTCATGATGGCTACTCACGGTGGTAGAGAACGAACAACTGTTGAGTTTGACGACCTATCAAAAGCTGCAGGCTTTGTTGAAACAAAGATTTTTTCGATTTCAAATGGGTGTTATGTTATGGAGTTTCACAAAGTTGAAACATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

362

Amino Acids

40.39

Weight (kDa)

5.75

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 22 - 103 5e-11 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 135 - 339 2.6e-56 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 432
AccB7I CCANNNNNTGG 1 cut(s) 857
AclI AACGTT 1 cut(s) 684
AclWI GGATC 1 cut(s) 529
AcoI YGGCCR 1 cut(s) 523
AcsI RAATTY 2 cut(s) 46, 202
AfaI GTAC 7 cut(s) 129, 252, 383, 420, 476, 532, 789
AfeI AGCGCT 1 cut(s) 833
AfiI CCNNNNNNNGG 1 cut(s) 857
AflIII ACRYGT 1 cut(s) 477
AhdI GACNNNNNGTC 1 cut(s) 297
AjnI CCWGG 1 cut(s) 427
Alw21I GWGCWC 2 cut(s) 99, 139
Alw26I GTCTC 3 cut(s) 40, 284, 305
AlwI GGATC 1 cut(s) 529
Aor51HI AGCGCT 1 cut(s) 833
AoxI GGCC 2 cut(s) 257, 523
ApeKI GCWGC 4 cut(s) 86, 194, 197, 1007
ApoI RAATTY 2 cut(s) 46, 202
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
AspLEI GCGC 1 cut(s) 834
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 2 cut(s) 400, 623
BalI TGGCCA 1 cut(s) 525
BanI GGYRCC 1 cut(s) 432
BanII GRGCYC 3 cut(s) 58, 99, 139
BbsI GAAGAC 1 cut(s) 97
Bbv12I GWGCWC 2 cut(s) 99, 139
BbvI GCAGC 4 cut(s) 73, 184, 206, 994
BccI CCATC 4 cut(s) 332, 589, 608, 943
BciT130I CCWGG 1 cut(s) 429
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 3 cut(s) 40, 284, 305
BfaI CTAG 6 cut(s) 59, 323, 584, 642, 705, 899
BfmI CTRYAG 1 cut(s) 1008
BfoI RGCGCY 1 cut(s) 835
BisI GCNGC 4 cut(s) 87, 195, 198, 1008
BlsI GCNGC 4 cut(s) 88, 196, 199, 1009
BmcAI AGTACT 1 cut(s) 420
Bme1390I CCNGG 2 cut(s) 132, 429
BmeRI GACNNNNNGTC 1 cut(s) 297
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 2 cut(s) 132, 429
BmrI ACTGGG 1 cut(s) 808
BmuI ACTGGG 1 cut(s) 808
BpiI GAAGAC 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 126, 158
Bpu10I CCTNAGC 2 cut(s) 349, 497
BpuEI CTTGAG 1 cut(s) 678
BpuMI CCSGG 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 428
Bsc4I CCNNNNNNNGG 1 cut(s) 857
Bse1I ACTGG 2 cut(s) 803, 862
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 428
BseGI GGATG 1 cut(s) 604
BseLI CCNNNNNNNGG 1 cut(s) 857
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 2 cut(s) 474, 488
BseNI ACTGG 2 cut(s) 803, 862
BseRI GAGGAG 1 cut(s) 621
BseXI GCAGC 4 cut(s) 73, 184, 206, 994
BshFI GGCC 2 cut(s) 259, 525
BshNI GGYRCC 1 cut(s) 432
BsiHKAI GWGCWC 2 cut(s) 99, 139
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 1 cut(s) 857
BsmAI GTCTC 3 cut(s) 40, 284, 305
BsmFI GGGAC 1 cut(s) 815
BsnI GGCC 2 cut(s) 259, 525
Bsp1286I GDGCHC 3 cut(s) 58, 99, 139
Bsp1407I TGTACA 2 cut(s) 250, 381
Bsp143I GATC 2 cut(s) 534, 808
BspANI GGCC 2 cut(s) 259, 525
BspCNI CTCAG 2 cut(s) 475, 489
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 1 cut(s) 434
BspMAI CTGCAG 1 cut(s) 1012
BspPI GGATC 1 cut(s) 529
BspQI GCTCTTC 1 cut(s) 573
BspT107I GGYRCC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 114
BsrGI TGTACA 2 cut(s) 250, 381
BsrI ACTGG 2 cut(s) 803, 862
BssECI CCNNGG 1 cut(s) 428
BssMI GATC 2 cut(s) 534, 808
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 4 cut(s) 418, 768, 962, 982
Bst6I CTCTTC 5 cut(s) 282, 543, 573, 582, 906
BstAUI TGTACA 2 cut(s) 250, 381
BstC8I GCNNGC 1 cut(s) 1012
BstDEI CTNAG 6 cut(s) 177, 218, 227, 349, 483, 497
BstF5I GGATG 1 cut(s) 604
BstH2I RGCGCY 1 cut(s) 835
BstHHI GCGC 1 cut(s) 834
BstKTI GATC 2 cut(s) 537, 811
BstMAI GTCTC 3 cut(s) 40, 284, 305
BstMBI GATC 2 cut(s) 534, 808
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 429
BstNSI RCATGY 1 cut(s) 481
BstSCI CCNGG 2 cut(s) 130, 427
BstSFI CTRYAG 1 cut(s) 1008
BstV1I GCAGC 4 cut(s) 73, 184, 206, 994
BstV2I GAAGAC 1 cut(s) 97
BsuRI GGCC 2 cut(s) 259, 525
BtsCI GGATG 1 cut(s) 604
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 1 cut(s) 1012
CciI TCATGA 1 cut(s) 945
CfoI GCGC 1 cut(s) 834
Csp6I GTAC 7 cut(s) 128, 251, 382, 419, 475, 531, 788
CviAII CATG 6 cut(s) 385, 403, 478, 659, 940, 946
CviQI GTAC 7 cut(s) 128, 251, 382, 419, 475, 531, 788
DdeI CTNAG 6 cut(s) 177, 218, 227, 349, 483, 497
DpnI GATC 2 cut(s) 536, 810
DpnII GATC 2 cut(s) 534, 808
DriI GACNNNNNGTC 1 cut(s) 297
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 5 cut(s) 282, 543, 573, 582, 906
Eam1105I GACNNNNNGTC 1 cut(s) 297
EarI CTCTTC 5 cut(s) 282, 543, 573, 582, 906
Ecl136II GAGCTC 2 cut(s) 97, 137
Eco24I GRGCYC 3 cut(s) 58, 99, 139
Eco32I GATATC 2 cut(s) 647, 710
Eco47III AGCGCT 1 cut(s) 833
Eco53kI GAGCTC 2 cut(s) 97, 137
EcoICRI GAGCTC 2 cut(s) 97, 137
EcoRII CCWGG 1 cut(s) 427
EcoRV GATATC 2 cut(s) 647, 710
EcoT38I GRGCYC 3 cut(s) 58, 99, 139
FaeI CATG 6 cut(s) 388, 406, 481, 662, 943, 949
FaqI GGGAC 1 cut(s) 815
FatI CATG 6 cut(s) 384, 402, 477, 658, 939, 945
Fnu4HI GCNGC 4 cut(s) 87, 195, 198, 1008
FokI GGATG 1 cut(s) 611
FriOI GRGCYC 3 cut(s) 58, 99, 139
Fsp4HI GCNGC 4 cut(s) 87, 195, 198, 1008
FspBI CTAG 6 cut(s) 59, 323, 584, 642, 705, 899
GlaI GCGC 1 cut(s) 833
GluI GCNGC 4 cut(s) 87, 195, 198, 1008
HaeII RGCGCY 1 cut(s) 835
HaeIII GGCC 2 cut(s) 259, 525
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 834
Hin1II CATG 6 cut(s) 388, 406, 481, 662, 943, 949
Hin6I GCGC 1 cut(s) 832
HinP1I GCGC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 3 cut(s) 181, 222, 313
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 400, 623
Hpy166II GTNNAC 2 cut(s) 235, 565
Hpy188I TCNGA 3 cut(s) 81, 376, 484
Hpy188III TCNNGA 2 cut(s) 642, 946
Hpy8I GTNNAC 2 cut(s) 235, 565
HpyAV CCTTC 1 cut(s) 446
HpyCH4III ACNGT 4 cut(s) 418, 768, 962, 982
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 5 cut(s) 89, 283, 764, 884, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 6 cut(s) 177, 218, 227, 349, 483, 497
HpySE526I ACGT 1 cut(s) 684
Hsp92II CATG 6 cut(s) 388, 406, 481, 662, 943, 949
HspAI GCGC 1 cut(s) 832
Kzo9I GATC 2 cut(s) 534, 808
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 2 cut(s) 134, 358
Lsp1109I GCAGC 4 cut(s) 73, 184, 206, 994
MaeI CTAG 6 cut(s) 59, 323, 584, 642, 705, 899
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 1 cut(s) 64
MalI GATC 2 cut(s) 536, 810
MboI GATC 2 cut(s) 534, 808
MboII GAAGA 7 cut(s) 97, 299, 347, 560, 590, 599, 923
MfeI CAATTG 1 cut(s) 503
MhlI GDGCHC 3 cut(s) 58, 99, 139
MlsI TGGCCA 1 cut(s) 525
MluCI AATT 7 cut(s) 15, 46, 121, 156, 202, 503, 624
MluNI TGGCCA 1 cut(s) 525
MlyI GAGTC 2 cut(s) 231, 307
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 5 cut(s) 544, 548, 599, 602, 835
Mox20I TGGCCA 1 cut(s) 525
MscI TGGCCA 1 cut(s) 525
MseI TTAA 4 cut(s) 510, 557, 666, 917
MslI CAYNNNNRTG 3 cut(s) 71, 657, 944
Msp20I TGGCCA 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 132, 429
MunI CAATTG 1 cut(s) 503
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 1 cut(s) 83
NciI CCSGG 1 cut(s) 132
NdeII GATC 2 cut(s) 534, 808
NlaIII CATG 6 cut(s) 388, 406, 481, 662, 943, 949
NlaIV GGNNCC 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 64
NspI RCATGY 1 cut(s) 481
PagI TCATGA 1 cut(s) 945
PciI ACATGT 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 573
PfeI GAWTC 1 cut(s) 181
PflFI GACNNNGTC 1 cut(s) 941
PflMI CCANNNNNTGG 1 cut(s) 857
PkrI GCNGC 4 cut(s) 88, 196, 199, 1009
PleI GAGTC 2 cut(s) 230, 307
PpsI GAGTC 2 cut(s) 230, 307
PscI ACATGT 1 cut(s) 477
Psp124BI GAGCTC 2 cut(s) 99, 139
Psp1406I AACGTT 1 cut(s) 684
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 1 cut(s) 434
PsrI GAACNNNNNNTAC 2 cut(s) 870, 902
PstI CTGCAG 1 cut(s) 1012
PsyI GACNNNGTC 1 cut(s) 941
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 7 cut(s) 129, 252, 383, 420, 476, 532, 789
RsaNI GTAC 7 cut(s) 128, 251, 382, 419, 475, 531, 788
RseI CAYNNNNRTG 3 cut(s) 71, 657, 944
SacI GAGCTC 2 cut(s) 99, 139
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 4 cut(s) 510, 557, 666, 917
SatI GCNGC 4 cut(s) 87, 195, 198, 1008
Sau3AI GATC 2 cut(s) 534, 808
ScaI AGTACT 1 cut(s) 420
SchI GAGTC 2 cut(s) 231, 307
ScrFI CCNGG 2 cut(s) 132, 429
SduI GDGCHC 3 cut(s) 58, 99, 139
SfcI CTRYAG 1 cut(s) 1008
SmiMI CAYNNNNRTG 3 cut(s) 71, 657, 944
SmlI CTYRAG 1 cut(s) 693
SmoI CTYRAG 1 cut(s) 693
Sse9I AATT 7 cut(s) 15, 46, 121, 156, 202, 503, 624
SspI AATATT 1 cut(s) 825
SspMI CTAG 6 cut(s) 59, 323, 584, 642, 705, 899
SstI GAGCTC 2 cut(s) 99, 139
StyD4I CCNGG 2 cut(s) 130, 427
TaaI ACNGT 4 cut(s) 418, 768, 962, 982
TaiI ACGT 1 cut(s) 687
TaqI TCGA 6 cut(s) 93, 149, 540, 672, 770, 1037
TasI AATT 7 cut(s) 15, 46, 121, 156, 202, 503, 624
TatI WGTACW 4 cut(s) 250, 381, 418, 474
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 4 cut(s) 510, 557, 666, 917
Tru9I TTAA 4 cut(s) 510, 557, 666, 917
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 4 cut(s) 86, 194, 197, 1007
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 3 cut(s) 348, 647, 782
TspRI CASTG 1 cut(s) 73
Tth111I GACNNNGTC 1 cut(s) 941
Van91I CCANNNNNTGG 1 cut(s) 857
XapI RAATTY 2 cut(s) 46, 202
XbaI TCTAGA 1 cut(s) 641
XceI RCATGY 1 cut(s) 481
XspI CTAG 6 cut(s) 59, 323, 584, 642, 705, 899
ZrmI AGTACT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.