Rh5AG114200
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000656
Physical Location & Seq
Forward (+)
10562508 .. 10624249
61742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG114200.1

Sequence Viewer

Length: 558 bp
ATGGAAAGTCATGGAAGTTTAGAAAATCATACTTCAGCTTGGGGTTTGGCAAATTCCATCACCATTCAAATGACTCTGAGAGCTGCAGTTGAGGTCAATGTGTTCAACATCATTGCCAAATCAGGTCCCGGAGCTCATCTGACATCAAAAGAAATTGTTTCACAAATCCCCAACCCAAATCCAACTTTAGCAGGTGAAAATCTGGAGCGAATGCTCAAACTTTTGAGTGTTCATTCTCTTTTATCCACATCTGTAAGACTAAACCCTACTGATACGACAAAGCAAGAAACAAGTTATGGGCTAACAAAGGAAGCACTTTGCCTATTCCCGAATAAAGATGGAGTTTCTTTTGCTCCGGTCGTTGTGTTGGGCTCCGAAATGCTCGTTGCAAAGAGCTTCTATATGCTCAAGTACAAGGTGCTTGAACCGGAGAGTTCTCCATTTCTCAAAGCCCATGGAGAGAGTTTTTATGACTACATGTCTAACAAGCCTGAGCTGTGCCAATTGTTTGACAAGGTGATGGAAGTAAATTCTTACCTATATTTTGATCGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00950 ko01110 map00950 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

185

Amino Acids

20.52

Weight (kDa)

5.95

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 14 - 106 6.5e-10 O-methyltransferase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 182
Acc36I ACCTGC 1 cut(s) 182
AcsI RAATTY 2 cut(s) 52, 529
AcuI CTGAAG 1 cut(s) 18
AfaI GTAC 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 3 cut(s) 68, 106, 425
AhdI GACNNNNNGTC 1 cut(s) 478
AluBI AGCT 5 cut(s) 38, 83, 134, 396, 496
AluI AGCT 5 cut(s) 38, 83, 134, 396, 496
Alw21I GWGCWC 1 cut(s) 136
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 2 cut(s) 52, 529
AspS9I GGNCC 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 3 cut(s) 52, 206, 529
AvaII GGWCC 1 cut(s) 125
BanII GRGCYC 2 cut(s) 136, 374
Bbv12I GWGCWC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 70
BccI CCATC 3 cut(s) 65, 332, 514
BcnI CCSGG 1 cut(s) 129
BfmI CTRYAG 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 182
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 1 cut(s) 129
Bme18I GGWCC 1 cut(s) 125
BmeRI GACNNNNNGTC 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 127, 373
BmrFI CCNGG 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 224
Bpu10I CCTNAGC 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 129
Bsa29I ATCGAT 1 cut(s) 550
BsaJI CCNNGG 1 cut(s) 454
BsaWI WCCGGW 2 cut(s) 355, 427
Bse3DI GCAATG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 550
BseDI CCNNGG 1 cut(s) 454
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 68, 483
BseXI GCAGC 1 cut(s) 70
Bsh1285I CGRYCG 1 cut(s) 360
BshVI ATCGAT 1 cut(s) 550
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 136
BsiSI CCGG 3 cut(s) 129, 356, 428
BslFI GGGAC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 111
BsmI GAATGC 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 136, 374
Bsp143I GATC 2 cut(s) 547, 551
Bsp19I CCATGG 1 cut(s) 454
BspCNI CTCAG 2 cut(s) 69, 484
BspDI ATCGAT 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 127, 373
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 454
BssMI GATC 2 cut(s) 547, 551
BssT1I CCWWGG 1 cut(s) 454
BstDEI CTNAG 2 cut(s) 77, 492
BstDSI CCRYGG 1 cut(s) 454
BstKTI GATC 2 cut(s) 550, 554
BstMBI GATC 2 cut(s) 547, 551
BstMCI CGRYCG 1 cut(s) 360
BstNSI RCATGY 1 cut(s) 481
BstSCI CCNGG 1 cut(s) 127
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 70
Bsu15I ATCGAT 1 cut(s) 550
BsuTUI ATCGAT 1 cut(s) 550
BtgI CCRYGG 1 cut(s) 454
BveI ACCTGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 125
ClaI ATCGAT 1 cut(s) 550
Csp6I GTAC 1 cut(s) 412
CviAII CATG 3 cut(s) 11, 455, 478
CviJI RGCY 9 cut(s) 38, 83, 134, 301, 372, 396, 452, 490, 496
CviKI_1 RGCY 9 cut(s) 38, 83, 134, 301, 372, 396, 452, 490, 496
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 2 cut(s) 77, 492
DpnI GATC 2 cut(s) 549, 553
DpnII GATC 2 cut(s) 547, 551
DriI GACNNNNNGTC 1 cut(s) 478
Eam1105I GACNNNNNGTC 1 cut(s) 478
Ecl136II GAGCTC 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 2 cut(s) 136, 374
Eco47I GGWCC 1 cut(s) 125
Eco53kI GAGCTC 1 cut(s) 134
Eco57I CTGAAG 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 454
EcoT38I GRGCYC 2 cut(s) 136, 374
ErhI CCWWGG 1 cut(s) 454
FaeI CATG 3 cut(s) 14, 458, 481
FaqI GGGAC 1 cut(s) 111
FatI CATG 3 cut(s) 10, 454, 477
Fnu4HI GCNGC 1 cut(s) 84
FriOI GRGCYC 2 cut(s) 136, 374
Fsp4HI GCNGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 224
HapII CCGG 3 cut(s) 129, 356, 428
Hin1II CATG 3 cut(s) 14, 458, 481
HinfI GANTC 1 cut(s) 73
HpaII CCGG 3 cut(s) 129, 356, 428
HphI GGTGA 3 cut(s) 52, 206, 529
Hpy188I TCNGA 3 cut(s) 78, 141, 376
Hpy188III TCNNGA 2 cut(s) 203, 328
HpyCH4V TGCA 2 cut(s) 86, 389
HpyF3I CTNAG 2 cut(s) 77, 492
Hsp92II CATG 3 cut(s) 14, 458, 481
Kzo9I GATC 2 cut(s) 547, 551
LmnI GCTCC 4 cut(s) 131, 205, 358, 377
LpnPI CCDG 7 cut(s) 108, 142, 177, 188, 369, 441, 504
Lsp1109I GCAGC 1 cut(s) 70
MalI GATC 2 cut(s) 549, 553
MboI GATC 2 cut(s) 547, 551
MfeI CAATTG 1 cut(s) 503
MhlI GDGCHC 2 cut(s) 136, 374
MluCI AATT 4 cut(s) 52, 153, 503, 529
MlyI GAGTC 1 cut(s) 67
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 1 cut(s) 85
MslI CAYNNNNRTG 1 cut(s) 68
MspI CCGG 3 cut(s) 129, 356, 428
MspR9I CCNGG 1 cut(s) 129
MunI CAATTG 1 cut(s) 503
Mva1269I GAATGC 1 cut(s) 216
NciI CCSGG 1 cut(s) 129
NcoI CCATGG 1 cut(s) 454
NdeII GATC 2 cut(s) 547, 551
NlaIII CATG 3 cut(s) 14, 458, 481
NlaIV GGNNCC 2 cut(s) 127, 373
NspI RCATGY 1 cut(s) 481
PaqCI CACCTGC 1 cut(s) 182
PciI ACATGT 1 cut(s) 477
PctI GAATGC 1 cut(s) 216
PfoI TCCNGGA 1 cut(s) 127
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
PpuMI RGGWCCY 1 cut(s) 125
PscI ACATGT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 125
PspN4I GGNNCC 2 cut(s) 127, 373
PspPI GGNCC 1 cut(s) 125
PspPPI RGGWCCY 1 cut(s) 125
PstI CTGCAG 1 cut(s) 88
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 1 cut(s) 68
SacI GAGCTC 1 cut(s) 136
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 547, 551
Sau96I GGNCC 1 cut(s) 125
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 2 cut(s) 136, 374
SfcI CTRYAG 1 cut(s) 84
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 4 cut(s) 52, 153, 503, 529
SstI GAGCTC 1 cut(s) 136
StyD4I CCNGG 1 cut(s) 127
StyI CCWWGG 1 cut(s) 454
TaqI TCGA 1 cut(s) 550
TasI AATT 4 cut(s) 52, 153, 503, 529
TatI WGTACW 1 cut(s) 411
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 2 cut(s) 52, 529
XceI RCATGY 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.