Rh5BG115000
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000656
Physical Location & Seq
Forward (+)
10751926 .. 10753500
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG115000.1

Sequence Viewer

Length: 1086 bp
ATGGAAACCCAAGGAAGTCTGGAAAATTATGTCTCAGTTTGGAATATGGGAAATTCGGTTTGCATTCAAATGGCTCTAAGAGCTGCAATAGAGCTCGATGTCTTCAATATCATTGCTAAGTCAGGGCCAGAAGCTCATCTTACATCAAAAGAAATTGTTTCGGCAATCCCCACCACAAACCCAAATGTAGCAGCTAAGAATTTAGAGCGAATCCTTAAGCTTCTTAGTGTCAATTCCCTCCTTTCTACCTCTCTCAAACCAAGCCTAAATGACAAAACACCACAAGAAAGGGCTTATGGCCTAACAAAAAATGCACTTTGCCTGGTGCCAAATGAAGATGGAGTTTCTATCGCACCAATAATTATGTCGGGCTCTGAAATGCACGTTATACAGAGCTTATACATGCTCAAGCACTCAGTGCTTGAGCCAGAGAGTATTCCTTTCTATAAATCCCACGGAGAAAATTTTTATGAGTACATGTCCCAGAGACCTGAGACCTTTTCATTGTTCAACAAGCTCATGAGAACAACCTCTTATATATATTTTGATCAGAAGGTGCTTAAGGTTTATCGAGGTTTTGAAGAAGTGAAAGAGCTTATGGATGTAGGGGGTGGTGATGGAAGTTCCCTTGCAAAAATTGTGTCTATGTATCCTCACATCCATGGCATCAATTTCAACTTACCTAGTGTTATTGCTCAGGCTCCTAAATATCAAGGTGTGAAGCATGTCAGTGGAGATATGTTTGACTCGATACCAAAAACACAGTCAATTATATTGAAGTGGGTACTTCATAACTGGGATGATGAGCATTGCAAGAAGATATTGAGAAACTGTTGGGAGGCGTTACCAGAAACCGGAAAGGTTATAGTTGTGGAATATTTCATGCTTCCCGAAGAATTGGAGAATACCCCAGAAACAATGCAGATATTAAAGGGTGACGTTATCATGATGACAATTTTTAATGGTAAGGAGCGAACAATAGCTGAAATTGATAACCTAGCCAAATGTGTAGGGTTTATTGAAACAAAGATTGTCCATGTTTCTCATGGAACATATGTTATCGAGTTCCTCAAGAAAAAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

361

Amino Acids

40.7

Weight (kDa)

6.19

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 18 - 109 4.9e-11 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 136 - 339 3.9e-53 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 325
AcsI RAATTY 3 cut(s) 52, 199, 463
AdeI CACNNNGTG 1 cut(s) 418
AfaI GTAC 2 cut(s) 476, 786
AfiI CCNNNNNNNGG 1 cut(s) 854
AflII CTTAAG 2 cut(s) 215, 560
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 7 cut(s) 68, 106, 511, 581, 676, 778, 1022
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 9 cut(s) 83, 94, 134, 194, 220, 396, 517, 595, 983
AluI AGCT 9 cut(s) 83, 94, 134, 194, 220, 396, 517, 595, 983
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 3 cut(s) 37, 481, 488
AoxI GGCC 2 cut(s) 125, 298
ApeKI GCWGC 2 cut(s) 83, 191
ApoI RAATTY 3 cut(s) 52, 199, 463
ArsI GACNNNNNNTTYG 4 cut(s) 626, 658, 749, 781
AspS9I GGNCC 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 626, 947
BanI GGYRCC 1 cut(s) 325
BanII GRGCYC 2 cut(s) 96, 374
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 70, 203
BccI CCATC 2 cut(s) 332, 611
BciT130I CCWGG 1 cut(s) 323
BciVI GTATCC 1 cut(s) 660
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 3 cut(s) 37, 481, 488
BfaI CTAG 2 cut(s) 684, 998
BfrI CTTAAG 2 cut(s) 215, 560
BfuI GTATCC 1 cut(s) 660
BisI GCNGC 2 cut(s) 84, 192
BlsI GCNGC 2 cut(s) 85, 193
Bme1390I CCNGG 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 327, 702
BmrFI CCNGG 1 cut(s) 323
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 1 cut(s) 675
BmuI ACTGGG 1 cut(s) 805
BpiI GAAGAC 1 cut(s) 94
Bpu10I CCTNAGC 1 cut(s) 696
BpuEI CTTGAG 3 cut(s) 392, 443, 1055
BsaI GGTCTC 2 cut(s) 481, 488
BsaJI CCNNGG 3 cut(s) 10, 454, 661
BsaWI WCCGGW 1 cut(s) 854
Bsc4I CCNNNNNNNGG 1 cut(s) 854
Bse1I ACTGG 1 cut(s) 800
Bse3DI GCAATG 2 cut(s) 111, 808
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 3 cut(s) 10, 454, 661
BseGI GGATG 3 cut(s) 607, 657, 805
BseLI CCNNNNNNNGG 1 cut(s) 854
BseMI GCAATG 2 cut(s) 111, 808
BseMII CTCAG 4 cut(s) 48, 429, 483, 710
BseNI ACTGG 1 cut(s) 800
BseXI GCAGC 2 cut(s) 70, 203
BshFI GGCC 2 cut(s) 127, 300
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 1 cut(s) 855
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 1 cut(s) 854
BsmAI GTCTC 3 cut(s) 37, 481, 488
BsmFI GGGAC 1 cut(s) 466
BsmI GAATGC 1 cut(s) 63
BsnI GGCC 2 cut(s) 127, 300
Bso31I GGTCTC 2 cut(s) 481, 488
Bsp1286I GDGCHC 2 cut(s) 96, 374
Bsp143I GATC 1 cut(s) 547
Bsp19I CCATGG 1 cut(s) 661
BspANI GGCC 2 cut(s) 127, 300
BspCNI CTCAG 4 cut(s) 47, 428, 484, 709
BspHI TCATGA 2 cut(s) 519, 945
BspLI GGNNCC 2 cut(s) 327, 702
BspT107I GGYRCC 1 cut(s) 325
BspTI CTTAAG 2 cut(s) 215, 560
BspTNI GGTCTC 2 cut(s) 481, 488
BsrDI GCAATG 2 cut(s) 111, 808
BsrI ACTGG 1 cut(s) 800
BssECI CCNNGG 3 cut(s) 10, 454, 661
BssMI GATC 1 cut(s) 547
BssT1I CCWWGG 2 cut(s) 10, 661
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 2 cut(s) 765, 833
BstAFI CTTAAG 2 cut(s) 215, 560
BstAPI GCANNNNNTGC 1 cut(s) 418
BstDEI CTNAG 8 cut(s) 34, 77, 117, 195, 224, 415, 492, 696
BstDSI CCRYGG 2 cut(s) 454, 661
BstF5I GGATG 3 cut(s) 607, 657, 805
BstKTI GATC 1 cut(s) 550
BstMAI GTCTC 3 cut(s) 37, 481, 488
BstMBI GATC 1 cut(s) 547
BstMWI GCNNNNNNNGC 2 cut(s) 80, 418
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 3 cut(s) 406, 481, 728
BstSCI CCNGG 1 cut(s) 321
BstV1I GCAGC 2 cut(s) 70, 203
BstV2I GAAGAC 1 cut(s) 94
BsuI GTATCC 1 cut(s) 660
BsuRI GGCC 2 cut(s) 127, 300
BtgI CCRYGG 2 cut(s) 454, 661
BtsCI GGATG 3 cut(s) 607, 657, 805
BtsIMutI CAGTG 2 cut(s) 423, 736
CciI TCATGA 2 cut(s) 519, 945
Cfr13I GGNCC 1 cut(s) 125
Csp6I GTAC 2 cut(s) 475, 785
CviAII CATG 9 cut(s) 403, 478, 520, 662, 725, 883, 946, 1037, 1046
CviQI GTAC 2 cut(s) 475, 785
DdeI CTNAG 8 cut(s) 34, 77, 117, 195, 224, 415, 492, 696
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
DraIII CACNNNGTG 1 cut(s) 418
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 10, 661
Eco24I GRGCYC 2 cut(s) 96, 374
Eco31I GGTCTC 2 cut(s) 481, 488
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 2 cut(s) 10, 661
EcoT38I GRGCYC 2 cut(s) 96, 374
ErhI CCWWGG 2 cut(s) 10, 661
FaeI CATG 9 cut(s) 406, 481, 523, 665, 728, 886, 949, 1040, 1049
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 1 cut(s) 466
FatI CATG 9 cut(s) 402, 477, 519, 661, 724, 882, 945, 1036, 1045
FauNDI CATATG 1 cut(s) 1054
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 84, 192
FokI GGATG 3 cut(s) 614, 644, 812
FriOI GRGCYC 2 cut(s) 96, 374
Fsp4HI GCNGC 2 cut(s) 84, 192
FspBI CTAG 2 cut(s) 684, 998
GluI GCNGC 2 cut(s) 84, 192
HaeIII GGCC 2 cut(s) 127, 300
HapII CCGG 1 cut(s) 855
Hin1II CATG 9 cut(s) 406, 481, 523, 665, 728, 886, 949, 1040, 1049
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 2 cut(s) 210, 746
HpaII CCGG 1 cut(s) 855
HphI GGTGA 2 cut(s) 626, 947
Hpy188I TCNGA 2 cut(s) 376, 552
Hpy188III TCNNGA 5 cut(s) 20, 520, 890, 946, 1072
HpyAV CCTTC 1 cut(s) 547
HpyCH4III ACNGT 2 cut(s) 765, 833
HpyCH4IV ACGT 2 cut(s) 384, 939
HpyCH4V TGCA 7 cut(s) 63, 86, 314, 382, 632, 813, 922
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 418
HpyF3I CTNAG 8 cut(s) 34, 77, 117, 195, 224, 415, 492, 696
HpySE526I ACGT 2 cut(s) 384, 939
Hsp92II CATG 9 cut(s) 406, 481, 523, 665, 728, 886, 949, 1040, 1049
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 1 cut(s) 547
LmnI GCTCC 2 cut(s) 706, 970
Lsp1109I GCAGC 2 cut(s) 70, 203
LweI GCATC 1 cut(s) 675
MaeI CTAG 2 cut(s) 684, 998
MaeII ACGT 2 cut(s) 384, 939
MaeIII GTNAC 2 cut(s) 843, 935
MalI GATC 1 cut(s) 549
MboI GATC 1 cut(s) 547
MboII GAAGA 5 cut(s) 94, 347, 593, 829, 905
MhlI GDGCHC 2 cut(s) 96, 374
MlyI GAGTC 1 cut(s) 740
MnlI CCTC 7 cut(s) 248, 259, 541, 566, 663, 832, 1079
MseI TTAA 5 cut(s) 216, 561, 929, 960, 1084
MslI CAYNNNNRTG 3 cut(s) 68, 660, 729
MspCI CTTAAG 2 cut(s) 215, 560
MspI CCGG 1 cut(s) 855
MspR9I CCNGG 1 cut(s) 323
Mva1269I GAATGC 1 cut(s) 63
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 80, 418
NcoI CCATGG 1 cut(s) 661
NdeI CATATG 1 cut(s) 1054
NdeII GATC 1 cut(s) 547
NlaIII CATG 9 cut(s) 406, 481, 523, 665, 728, 886, 949, 1040, 1049
NlaIV GGNNCC 2 cut(s) 327, 702
NmuCI GTSAC 1 cut(s) 935
NspI RCATGY 3 cut(s) 406, 481, 728
PagI TCATGA 2 cut(s) 519, 945
PciI ACATGT 1 cut(s) 477
PctI GAATGC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 210
PkrI GCNGC 2 cut(s) 85, 193
PleI GAGTC 1 cut(s) 740
PpsI GAGTC 1 cut(s) 740
PscI ACATGT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 2 cut(s) 327, 702
PspPI GGNCC 1 cut(s) 125
RsaI GTAC 2 cut(s) 476, 786
RsaNI GTAC 2 cut(s) 475, 785
RseI CAYNNNNRTG 3 cut(s) 68, 660, 729
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 5 cut(s) 216, 561, 929, 960, 1084
SatI GCNGC 2 cut(s) 84, 192
Sau3AI GATC 1 cut(s) 547
Sau96I GGNCC 1 cut(s) 125
SchI GAGTC 1 cut(s) 740
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 2 cut(s) 96, 374
SfaNI GCATC 1 cut(s) 675
SmiMI CAYNNNNRTG 3 cut(s) 68, 660, 729
SmlI CTYRAG 5 cut(s) 215, 407, 422, 560, 1070
SmoI CTYRAG 5 cut(s) 215, 407, 422, 560, 1070
SspI AATATT 1 cut(s) 878
SspMI CTAG 2 cut(s) 684, 998
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 2 cut(s) 10, 661
TaaI ACNGT 2 cut(s) 765, 833
TaiI ACGT 2 cut(s) 387, 942
TaqI TCGA 4 cut(s) 96, 571, 749, 1062
TatI WGTACW 1 cut(s) 474
TfiI GAWTC 1 cut(s) 210
Tru1I TTAA 5 cut(s) 216, 561, 929, 960, 1084
Tru9I TTAA 5 cut(s) 216, 561, 929, 960, 1084
TscAI CASTG 2 cut(s) 423, 736
TseFI GTSAC 1 cut(s) 935
TseI GCWGC 2 cut(s) 83, 191
Tsp45I GTSAC 1 cut(s) 935
TspDTI ATGAA 4 cut(s) 348, 492, 779, 871
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 2 cut(s) 423, 736
Vha464I CTTAAG 2 cut(s) 215, 560
XapI RAATTY 3 cut(s) 52, 199, 463
XceI RCATGY 3 cut(s) 406, 481, 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 1043
XspI CTAG 2 cut(s) 684, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.