Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 710 |
| AcoI | YGGCCR | 1 cut(s) | 526 |
| AcsI | RAATTY | 3 cut(s) | 46, 202, 505 |
| AdeI | CACNNNGTG | 1 cut(s) | 391 |
| AfaI | GTAC | 1 cut(s) | 535 |
| AflIII | ACRYGT | 1 cut(s) | 480 |
| AgsI | TTSAA | 5 cut(s) | 109, 208, 551, 581, 726 |
| AjnI | CCWGG | 1 cut(s) | 430 |
| Alw21I | GWGCWC | 4 cut(s) | 99, 139, 480, 501 |
| Alw26I | GTCTC | 1 cut(s) | 308 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 197 |
| AoxI | GGCC | 2 cut(s) | 294, 526 |
| ApeKI | GCWGC | 4 cut(s) | 86, 191, 194, 197 |
| ApoI | RAATTY | 3 cut(s) | 46, 202, 505 |
| AspS9I | GGNCC | 1 cut(s) | 128 |
| AsuC2I | CCSGG | 1 cut(s) | 132 |
| AvaII | GGWCC | 1 cut(s) | 128 |
| BalI | TGGCCA | 1 cut(s) | 528 |
| BanII | GRGCYC | 3 cut(s) | 99, 139, 501 |
| BbsI | GAAGAC | 1 cut(s) | 97 |
| Bbv12I | GWGCWC | 4 cut(s) | 99, 139, 480, 501 |
| BbvI | GCAGC | 4 cut(s) | 73, 184, 203, 206 |
| BccI | CCATC | 3 cut(s) | 335, 545, 592 |
| BciT130I | CCWGG | 1 cut(s) | 432 |
| BcnI | CCSGG | 1 cut(s) | 132 |
| BcoDI | GTCTC | 1 cut(s) | 308 |
| BfaI | CTAG | 4 cut(s) | 59, 287, 326, 645 |
| BfuAI | ACCTGC | 1 cut(s) | 710 |
| BisI | GCNGC | 4 cut(s) | 87, 192, 195, 198 |
| BlsI | GCNGC | 4 cut(s) | 88, 193, 196, 199 |
| Bme1390I | CCNGG | 2 cut(s) | 132, 432 |
| Bme18I | GGWCC | 1 cut(s) | 128 |
| BmgT120I | GGNCC | 1 cut(s) | 128 |
| BmiI | GGNNCC | 1 cut(s) | 130 |
| BmrFI | CCNGG | 2 cut(s) | 132, 432 |
| BpiI | GAAGAC | 1 cut(s) | 97 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 126, 158 |
| Bpu10I | CCTNAGC | 2 cut(s) | 352, 495 |
| BpuEI | CTTGAG | 3 cut(s) | 253, 446, 681 |
| BpuMI | CCSGG | 1 cut(s) | 132 |
| BsaJI | CCNNGG | 2 cut(s) | 431, 735 |
| Bse3DI | GCAATG | 3 cut(s) | 114, 622, 690 |
| BseBI | CCWGG | 1 cut(s) | 432 |
| BseDI | CCNNGG | 2 cut(s) | 431, 735 |
| BseGI | GGATG | 1 cut(s) | 607 |
| BseMI | GCAATG | 3 cut(s) | 114, 622, 690 |
| BseMII | CTCAG | 2 cut(s) | 241, 486 |
| BseRI | GAGGAG | 1 cut(s) | 624 |
| BseXI | GCAGC | 4 cut(s) | 73, 184, 203, 206 |
| BshFI | GGCC | 2 cut(s) | 296, 528 |
| BsiHKAI | GWGCWC | 4 cut(s) | 99, 139, 480, 501 |
| BsiSI | CCGG | 1 cut(s) | 132 |
| BsmAI | GTCTC | 1 cut(s) | 308 |
| BsnI | GGCC | 2 cut(s) | 296, 528 |
| Bsp1286I | GDGCHC | 4 cut(s) | 99, 139, 480, 501 |
| BspANI | GGCC | 2 cut(s) | 296, 528 |
| BspCNI | CTCAG | 2 cut(s) | 240, 487 |
| BspLI | GGNNCC | 1 cut(s) | 130 |
| BspMI | ACCTGC | 1 cut(s) | 710 |
| BsrDI | GCAATG | 3 cut(s) | 114, 622, 690 |
| BssECI | CCNNGG | 2 cut(s) | 431, 735 |
| BssT1I | CCWWGG | 1 cut(s) | 735 |
| Bst2UI | CCWGG | 1 cut(s) | 432 |
| Bst4CI | ACNGT | 1 cut(s) | 421 |
| Bst6I | CTCTTC | 2 cut(s) | 285, 576 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 197 |
| BstDEI | CTNAG | 4 cut(s) | 218, 227, 352, 495 |
| BstF5I | GGATG | 1 cut(s) | 607 |
| BstMAI | GTCTC | 1 cut(s) | 308 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 83, 197 |
| BstNI | CCWGG | 1 cut(s) | 432 |
| BstNSI | RCATGY | 1 cut(s) | 484 |
| BstSCI | CCNGG | 2 cut(s) | 130, 430 |
| BstV1I | GCAGC | 4 cut(s) | 73, 184, 203, 206 |
| BstV2I | GAAGAC | 1 cut(s) | 97 |
| BsuRI | GGCC | 2 cut(s) | 296, 528 |
| BtsCI | GGATG | 1 cut(s) | 607 |
| BtsIMutI | CAGTG | 2 cut(s) | 235, 426 |
| BveI | ACCTGC | 1 cut(s) | 710 |
| CaiI | CAGNNNCTG | 1 cut(s) | 197 |
| Cfr13I | GGNCC | 1 cut(s) | 128 |
| Csp6I | GTAC | 1 cut(s) | 534 |
| CviAII | CATG | 4 cut(s) | 406, 458, 481, 662 |
| CviQI | GTAC | 1 cut(s) | 534 |
| DdeI | CTNAG | 4 cut(s) | 218, 227, 352, 495 |
| DraI | TTTAAA | 1 cut(s) | 81 |
| DraIII | CACNNNGTG | 1 cut(s) | 391 |
| EaeI | YGGCCR | 1 cut(s) | 526 |
| Eam1104I | CTCTTC | 2 cut(s) | 285, 576 |
| EarI | CTCTTC | 2 cut(s) | 285, 576 |
| Ecl136II | GAGCTC | 3 cut(s) | 97, 137, 499 |
| Eco130I | CCWWGG | 1 cut(s) | 735 |
| Eco147I | AGGCCT | 1 cut(s) | 296 |
| Eco24I | GRGCYC | 3 cut(s) | 99, 139, 501 |
| Eco47I | GGWCC | 1 cut(s) | 128 |
| Eco53kI | GAGCTC | 3 cut(s) | 97, 137, 499 |
| EcoICRI | GAGCTC | 3 cut(s) | 97, 137, 499 |
| EcoO109I | RGGNCCY | 1 cut(s) | 128 |
| EcoRII | CCWGG | 1 cut(s) | 430 |
| EcoT14I | CCWWGG | 1 cut(s) | 735 |
| EcoT38I | GRGCYC | 3 cut(s) | 99, 139, 501 |
| ErhI | CCWWGG | 1 cut(s) | 735 |
| FaeI | CATG | 4 cut(s) | 409, 461, 484, 665 |
| FatI | CATG | 4 cut(s) | 405, 457, 480, 661 |
| FauNDI | CATATG | 1 cut(s) | 472 |
| Fnu4HI | GCNGC | 4 cut(s) | 87, 192, 195, 198 |
| FokI | GGATG | 1 cut(s) | 614 |
| FriOI | GRGCYC | 3 cut(s) | 99, 139, 501 |
| Fsp4HI | GCNGC | 4 cut(s) | 87, 192, 195, 198 |
| FspBI | CTAG | 4 cut(s) | 59, 287, 326, 645 |
| GluI | GCNGC | 4 cut(s) | 87, 192, 195, 198 |
| HaeIII | GGCC | 2 cut(s) | 296, 528 |
| HapII | CCGG | 1 cut(s) | 132 |
| Hin1II | CATG | 4 cut(s) | 409, 461, 484, 665 |
| HinfI | GANTC | 2 cut(s) | 316, 740 |
| HpaII | CCGG | 1 cut(s) | 132 |
| Hpy166II | GTNNAC | 1 cut(s) | 568 |
| Hpy188I | TCNGA | 2 cut(s) | 379, 487 |
| Hpy188III | TCNNGA | 2 cut(s) | 10, 126 |
| Hpy8I | GTNNAC | 1 cut(s) | 568 |
| HpyAV | CCTTC | 1 cut(s) | 445 |
| HpyCH4III | ACNGT | 1 cut(s) | 421 |
| HpyCH4V | TGCA | 4 cut(s) | 89, 191, 200, 385 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 83, 197 |
| HpyF3I | CTNAG | 4 cut(s) | 218, 227, 352, 495 |
| Hsp92II | CATG | 4 cut(s) | 409, 461, 484, 665 |
| LmnI | GCTCC | 3 cut(s) | 134, 361, 435 |
| LpnPI | CCDG | 7 cut(s) | 23, 111, 145, 417, 444, 507, 705 |
| Lsp1109I | GCAGC | 4 cut(s) | 73, 184, 203, 206 |
| MaeI | CTAG | 4 cut(s) | 59, 287, 326, 645 |
| MaeIII | GTNAC | 1 cut(s) | 64 |
| MboII | GAAGA | 5 cut(s) | 97, 302, 350, 563, 593 |
| MfeI | CAATTG | 1 cut(s) | 627 |
| MhlI | GDGCHC | 4 cut(s) | 99, 139, 480, 501 |
| MlsI | TGGCCA | 1 cut(s) | 528 |
| MluNI | TGGCCA | 1 cut(s) | 528 |
| MlyI | GAGTC | 1 cut(s) | 310 |
| MmeI | TCCRAC | 1 cut(s) | 586 |
| MnlI | CCTC | 6 cut(s) | 286, 478, 511, 577, 602, 605 |
| Mox20I | TGGCCA | 1 cut(s) | 528 |
| MscI | TGGCCA | 1 cut(s) | 528 |
| MseI | TTAA | 7 cut(s) | 80, 305, 447, 513, 560, 669, 747 |
| MslI | CAYNNNNRTG | 2 cut(s) | 71, 660 |
| Msp20I | TGGCCA | 1 cut(s) | 528 |
| MspA1I | CMGCKG | 1 cut(s) | 197 |
| MspI | CCGG | 1 cut(s) | 132 |
| MspR9I | CCNGG | 2 cut(s) | 132, 432 |
| MunI | CAATTG | 1 cut(s) | 627 |
| MvaI | CCWGG | 1 cut(s) | 432 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 83, 197 |
| NciI | CCSGG | 1 cut(s) | 132 |
| NdeI | CATATG | 1 cut(s) | 472 |
| NlaIII | CATG | 4 cut(s) | 409, 461, 484, 665 |
| NlaIV | GGNNCC | 1 cut(s) | 130 |
| NmuCI | GTSAC | 1 cut(s) | 64 |
| NspI | RCATGY | 1 cut(s) | 484 |
| PceI | AGGCCT | 1 cut(s) | 296 |
| PciI | ACATGT | 1 cut(s) | 480 |
| PfeI | GAWTC | 1 cut(s) | 740 |
| PkrI | GCNGC | 4 cut(s) | 88, 193, 196, 199 |
| PleI | GAGTC | 1 cut(s) | 310 |
| PpsI | GAGTC | 1 cut(s) | 310 |
| PpuMI | RGGWCCY | 1 cut(s) | 128 |
| PscI | ACATGT | 1 cut(s) | 480 |
| Psp124BI | GAGCTC | 3 cut(s) | 99, 139, 501 |
| Psp5II | RGGWCCY | 1 cut(s) | 128 |
| Psp6I | CCWGG | 1 cut(s) | 430 |
| PspGI | CCWGG | 1 cut(s) | 430 |
| PspN4I | GGNNCC | 1 cut(s) | 130 |
| PspPI | GGNCC | 1 cut(s) | 128 |
| PspPPI | RGGWCCY | 1 cut(s) | 128 |
| PstNI | CAGNNNCTG | 1 cut(s) | 197 |
| PvuII | CAGCTG | 1 cut(s) | 197 |
| RsaI | GTAC | 1 cut(s) | 535 |
| RsaNI | GTAC | 1 cut(s) | 534 |
| RseI | CAYNNNNRTG | 2 cut(s) | 71, 660 |
| SacI | GAGCTC | 3 cut(s) | 99, 139, 501 |
| SaqAI | TTAA | 7 cut(s) | 80, 305, 447, 513, 560, 669, 747 |
| SatI | GCNGC | 4 cut(s) | 87, 192, 195, 198 |
| Sau96I | GGNCC | 1 cut(s) | 128 |
| SchI | GAGTC | 1 cut(s) | 310 |
| ScrFI | CCNGG | 2 cut(s) | 132, 432 |
| SduI | GDGCHC | 4 cut(s) | 99, 139, 480, 501 |
| SinI | GGWCC | 1 cut(s) | 128 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 71, 660 |
| SmlI | CTYRAG | 3 cut(s) | 268, 425, 696 |
| SmoI | CTYRAG | 3 cut(s) | 268, 425, 696 |
| SseBI | AGGCCT | 1 cut(s) | 296 |
| SspMI | CTAG | 4 cut(s) | 59, 287, 326, 645 |
| SstI | GAGCTC | 3 cut(s) | 99, 139, 501 |
| StuI | AGGCCT | 1 cut(s) | 296 |
| StyD4I | CCNGG | 2 cut(s) | 130, 430 |
| StyI | CCWWGG | 1 cut(s) | 735 |
| TaaI | ACNGT | 1 cut(s) | 421 |
| TaqI | TCGA | 2 cut(s) | 93, 149 |
| TfiI | GAWTC | 1 cut(s) | 740 |
| Tru1I | TTAA | 7 cut(s) | 80, 305, 447, 513, 560, 669, 747 |
| Tru9I | TTAA | 7 cut(s) | 80, 305, 447, 513, 560, 669, 747 |
| TscAI | CASTG | 2 cut(s) | 235, 426 |
| TseFI | GTSAC | 1 cut(s) | 64 |
| TseI | GCWGC | 4 cut(s) | 86, 191, 194, 197 |
| Tsp45I | GTSAC | 1 cut(s) | 64 |
| TspDTI | ATGAA | 3 cut(s) | 224, 351, 650 |
| TspRI | CASTG | 2 cut(s) | 235, 426 |
| VpaK11BI | GGWCC | 1 cut(s) | 128 |
| XapI | RAATTY | 3 cut(s) | 46, 202, 505 |
| XceI | RCATGY | 1 cut(s) | 484 |
| XspI | CTAG | 4 cut(s) | 59, 287, 326, 645 |