Rw5G028650
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000656
Physical Location & Seq
Reverse (-)
43418316 .. 43420882
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028650.1

Sequence Viewer

Length: 558 bp
ATGACTTTAAGAGCTGCAGTAGAGCTCAATGTCTTCTCAGGGCGTGGAGTTCATCTCACATCAGAAGAAATCAAAAATCCGAGTGCAGCAATTGCGAATTTGGAGCGAATACTAAAACTGCTCAGCGTCAACTCCCTCCTTTCCGCATCTCTAAGACCAAGTGCAAATGACAACACAGTGCAAGAAGTGGCTTATGGCCTAACAAAGAAGGCACTTTGCCTAATTCCAAATGAAGATGGAGTTTCTTTAGCCCCTTTAGTAATGTATGGCTCTGAAACCTTGTATGAATATATGTCCGAGAAGCCTGAGCTGATTCAATTGTTTGATAAGGTAATGAGAATTACCAGTGTCCACCCAAGCTTGTATGTAGATCCGCCCCTAAGAATTACCTCTAACCTGTGTTTTGATCAGAATGTGCTTGAGGTTTATAGAGGCTTCAATGAGTTAAAAGAGCTAATGGATGTCGGGGGTGGTGACGGAAGTTCGATTGCAAAAATAGTGTCTATGCATCTTCATATCCATGGCATCAACTTCAATGTACCAAGTGTTATTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00950 ko01110 map00950 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

185

Amino Acids

20.27

Weight (kDa)

5.51

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 131 - 185 7.9e-08 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 144, 374
AclWI GGATC 1 cut(s) 365
AcsI RAATTY 1 cut(s) 97
AfaI GTAC 1 cut(s) 540
AgsI TTSAA 3 cut(s) 317, 439, 535
AluBI AGCT 5 cut(s) 14, 25, 310, 360, 454
AluI AGCT 5 cut(s) 14, 25, 310, 360, 454
Alw21I GWGCWC 1 cut(s) 27
AlwI GGATC 1 cut(s) 365
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 2 cut(s) 14, 86
ApoI RAATTY 1 cut(s) 97
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AsuHPI GGTGA 1 cut(s) 485
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 98
BccI CCATC 1 cut(s) 230
BclI TGATCA 1 cut(s) 406
BfmI CTRYAG 1 cut(s) 15
BisI GCNGC 2 cut(s) 15, 87
BlpI GCTNAGC 1 cut(s) 122
BlsI GCNGC 2 cut(s) 16, 88
BmsI GCATC 3 cut(s) 155, 517, 534
BpiI GAAGAC 1 cut(s) 25
BplI GAGNNNNNCTC 2 cut(s) 39, 71
Bpu10I CCTNAGC 1 cut(s) 306
Bpu1102I GCTNAGC 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 520
Bse1I ACTGG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 520
BseGI GGATG 1 cut(s) 466
BseMII CTCAG 3 cut(s) 51, 136, 297
BseNI ACTGG 1 cut(s) 345
BseXI GCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 105
BshFI GGCC 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 27
BsnI GGCC 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 370, 406
Bsp1720I GCTNAGC 1 cut(s) 122
Bsp19I CCATGG 1 cut(s) 520
BspACI CCGC 2 cut(s) 144, 374
BspANI GGCC 1 cut(s) 198
BspCNI CTCAG 3 cut(s) 50, 135, 298
BspMAI CTGCAG 1 cut(s) 19
BspPI GGATC 1 cut(s) 365
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 520
BssMI GATC 2 cut(s) 370, 406
BssT1I CCWWGG 1 cut(s) 520
Bst4CI ACNGT 1 cut(s) 178
BstAPI GCANNNNNTGC 1 cut(s) 92
BstDEI CTNAG 5 cut(s) 37, 122, 152, 306, 380
BstDSI CCRYGG 1 cut(s) 520
BstF5I GGATG 1 cut(s) 466
BstKTI GATC 2 cut(s) 373, 409
BstMBI GATC 2 cut(s) 370, 406
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 1 cut(s) 98
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 520
BtsCI GGATG 1 cut(s) 466
BtsIMutI CAGTG 2 cut(s) 183, 352
CseI GACGC 1 cut(s) 115
Csp6I GTAC 1 cut(s) 539
CviAII CATG 1 cut(s) 521
CviQI GTAC 1 cut(s) 539
DdeI CTNAG 5 cut(s) 37, 122, 152, 306, 380
DpnI GATC 2 cut(s) 372, 408
DpnII GATC 2 cut(s) 370, 406
EciI GGCGGA 1 cut(s) 363
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 520
EcoT22I ATGCAT 1 cut(s) 510
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 520
FaeI CATG 1 cut(s) 524
FatI CATG 1 cut(s) 520
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 2 cut(s) 15, 87
FokI GGATG 1 cut(s) 473
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 2 cut(s) 15, 87
GluI GCNGC 2 cut(s) 15, 87
HaeIII GGCC 1 cut(s) 198
HgaI GACGC 1 cut(s) 115
Hin1II CATG 1 cut(s) 524
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 1 cut(s) 313
HphI GGTGA 1 cut(s) 485
Hpy166II GTNNAC 2 cut(s) 130, 352
Hpy188I TCNGA 5 cut(s) 64, 81, 274, 298, 411
Hpy8I GTNNAC 2 cut(s) 130, 352
HpyAV CCTTC 1 cut(s) 202
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4V TGCA 6 cut(s) 17, 86, 164, 181, 491, 508
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpyF3I CTNAG 5 cut(s) 37, 122, 152, 306, 380
Hsp92II CATG 1 cut(s) 524
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 2 cut(s) 370, 406
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 4 cut(s) 24, 318, 358, 410
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 3 cut(s) 155, 517, 534
MaeIII GTNAC 1 cut(s) 473
MalI GATC 2 cut(s) 372, 408
MboI GATC 2 cut(s) 370, 406
MboII GAAGA 4 cut(s) 25, 77, 245, 503
MfeI CAATTG 2 cut(s) 90, 317
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 6 cut(s) 90, 97, 222, 317, 339, 384
MnlI CCTC 4 cut(s) 146, 400, 415, 425
Mph1103I ATGCAT 1 cut(s) 510
MseI TTAA 3 cut(s) 8, 446, 556
MslI CAYNNNNRTG 1 cut(s) 519
MunI CAATTG 2 cut(s) 90, 317
MwoI GCNNNNNNNGC 1 cut(s) 92
NcoI CCATGG 1 cut(s) 520
NdeII GATC 2 cut(s) 370, 406
NlaIII CATG 1 cut(s) 524
NmuCI GTSAC 1 cut(s) 473
NsiI ATGCAT 1 cut(s) 510
PfeI GAWTC 1 cut(s) 313
PkrI GCNGC 2 cut(s) 16, 88
Psp124BI GAGCTC 1 cut(s) 27
PstI CTGCAG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 1 cut(s) 540
RsaNI GTAC 1 cut(s) 539
RseI CAYNNNNRTG 1 cut(s) 519
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 3 cut(s) 8, 446, 556
SatI GCNGC 2 cut(s) 15, 87
Sau3AI GATC 2 cut(s) 370, 406
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 3 cut(s) 155, 517, 534
SfcI CTRYAG 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 519
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 6 cut(s) 90, 97, 222, 317, 339, 384
SsiI CCGC 2 cut(s) 144, 374
SstI GAGCTC 1 cut(s) 27
StyI CCWWGG 1 cut(s) 520
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 1 cut(s) 485
TasI AATT 6 cut(s) 90, 97, 222, 317, 339, 384
TfiI GAWTC 1 cut(s) 313
Tru1I TTAA 3 cut(s) 8, 446, 556
Tru9I TTAA 3 cut(s) 8, 446, 556
TscAI CASTG 2 cut(s) 183, 352
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 2 cut(s) 14, 86
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 4 cut(s) 41, 246, 300, 503
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 2 cut(s) 183, 352
XapI RAATTY 1 cut(s) 97
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.