Rh7DG266400
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000656
Physical Location & Seq
Forward (+)
28310342 .. 28312335
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG266400.1

Sequence Viewer

Length: 1092 bp
ATGGAAAACCAGAGAATTAGCTCAAAAAACTATGTCTCTGCTTGGAATTTGGGAGGTTTAGTTGTCACTCAGATGGCTCTAAAAGCTGCAATCGAACTCAATGTCTTCAATATCATAGCCAATTCAGGACCTGGAGCTCATCTCACATCGAAAGAAATTGTATCTCAAATCTCCACCATGAATCCAAATGCAGCAGCTGAGAATTTGAAGCGAATATTAAGAGTTCTAAGTGTGCATTCTTTGTTATCTATATCTCAAAGGCCAAGCTTAAATGGTAAGACAGTACTAGAAGAGACTTATGGTTTAACAAATGATACTCTTTGCCTAGTTCCAAATGAAGATGGAGTTTCCTTGGCTCCATATATTATCTTTGCTTCTGATATGACCCTTGTGAAAAGCTTGTCCATGCTCAAAGAAACAGTGCTTGAACCTGGGAGCATTCCTTTCAACAAGACTCATGGTGTGTCCATGTATGAGTACATGTCTGAGAAACCTGAGATGAGCCAATTGTTTAATAAGGCTATGGGACAAAGTTCCATCCTTGATTTTGAAGAGGTGTTAAATGTTTACAAAGGTTTTGAAGAGGTAAAAGAGTTGATGGATGTTGCAGGAGGCATTGGAACTACAATTGCCAAAGTAGTATCTAGGTATCCACACATTCAAGGAATTAACTTCGATTTACCTAATGTTGTCGCTCAAGGAATTGCTAAATACCAAGTAGGTGTGAAACATGTTGGTGGAGATATGTTTGAGTTGATACCAAATGCACAGTCTATTATGTTGAAGCGGATACTTCATAATTGGGATGATGATCTATGTAAGAAAATATTAAAATGTTGTTGGGAAGCATTACCAGAAATCGGGAAGGTAATAGTTGTGGAGTTTGCAATACCAGAAATAGTGGAGAAAACCGCAGATTTGGAGAAAATAGTGACATTAGACATCATAATGATGGCTTATCACGGCAGTAAAGAACGAACAATTGCTGAGTTTGACTACCTATCGAAAGCTGCAGGATTCAGTGAAATGAAGATTTTTCCGATTTCACATGGTTGTTATGTTATGGAACTTCACAAAGTTAAAACATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

363

Amino Acids

40.24

Weight (kDa)

5.77

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 21 - 103 1e-10 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 136 - 341 1.3e-52 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 787, 912
AcsI RAATTY 2 cut(s) 46, 202
AfaI GTAC 2 cut(s) 285, 479
AfiI CCNNNNNNNGG 1 cut(s) 860
AflIII ACRYGT 2 cut(s) 480, 730
AgsI TTSAA 8 cut(s) 109, 208, 428, 448, 551, 581, 662, 784
AjnI CCWGG 2 cut(s) 130, 430
AluBI AGCT 7 cut(s) 21, 86, 137, 197, 267, 399, 1010
AluI AGCT 7 cut(s) 21, 86, 137, 197, 267, 399, 1010
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 2 cut(s) 40, 287
AlwNI CAGNNNCTG 2 cut(s) 131, 197
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 4 cut(s) 86, 191, 194, 1010
ApoI RAATTY 2 cut(s) 46, 202
ArsI GACNNNNNNTTYG 4 cut(s) 87, 119, 755, 787
AspS9I GGNCC 1 cut(s) 128
AvaII GGWCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 97
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 4 cut(s) 73, 203, 206, 997
BccI CCATC 5 cut(s) 67, 335, 545, 592, 946
BceAI ACGGC 1 cut(s) 979
BciT130I CCWGG 2 cut(s) 132, 432
BciVI GTATCC 2 cut(s) 660, 783
BcoDI GTCTC 2 cut(s) 40, 287
BfaI CTAG 3 cut(s) 287, 326, 645
BfmI CTRYAG 1 cut(s) 1011
BfuI GTATCC 2 cut(s) 660, 783
BisI GCNGC 4 cut(s) 87, 192, 195, 1011
BlsI GCNGC 4 cut(s) 88, 193, 196, 1012
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 2 cut(s) 132, 432
Bme18I GGWCC 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 2 cut(s) 132, 432
BpiI GAAGAC 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 126, 158
BpmI CTGGAG 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 681
BsaBI GATNNNNATC 1 cut(s) 810
BsaJI CCNNGG 2 cut(s) 351, 431
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 1 cut(s) 860
Bse8I GATNNNNATC 1 cut(s) 810
BseBI CCWGG 2 cut(s) 132, 432
BseDI CCNNGG 2 cut(s) 351, 431
BseGI GGATG 3 cut(s) 537, 607, 811
BseJI GATNNNNATC 1 cut(s) 810
BseLI CCNNNNNNNGG 1 cut(s) 860
BseMII CTCAG 5 cut(s) 83, 189, 477, 486, 978
BseXI GCAGC 4 cut(s) 73, 203, 206, 997
BshFI GGCC 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 139
BslFI GGGAC 1 cut(s) 540
BslI CCNNNNNNNGG 1 cut(s) 860
BsmAI GTCTC 2 cut(s) 40, 287
BsmFI GGGAC 1 cut(s) 540
BsmI GAATGC 2 cut(s) 235, 438
BsnI GGCC 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 1 cut(s) 811
BspACI CCGC 2 cut(s) 787, 912
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 5 cut(s) 82, 190, 478, 487, 979
BspLI GGNNCC 1 cut(s) 357
BspMAI CTGCAG 1 cut(s) 1015
BssECI CCNNGG 2 cut(s) 351, 431
BssMI GATC 1 cut(s) 811
BssT1I CCWWGG 1 cut(s) 351
Bst2UI CCWGG 2 cut(s) 132, 432
Bst4CI ACNGT 3 cut(s) 283, 421, 771
Bst6I CTCTTC 3 cut(s) 285, 546, 576
BstDEI CTNAG 6 cut(s) 69, 198, 227, 486, 495, 987
BstF5I GGATG 3 cut(s) 537, 607, 811
BstKTI GATC 1 cut(s) 814
BstMAI GTCTC 2 cut(s) 40, 287
BstMBI GATC 1 cut(s) 811
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 2 cut(s) 132, 432
BstNSI RCATGY 2 cut(s) 484, 734
BstSCI CCNGG 2 cut(s) 130, 430
BstSFI CTRYAG 1 cut(s) 1011
BstV1I GCAGC 4 cut(s) 73, 203, 206, 997
BstV2I GAAGAC 1 cut(s) 97
BsuI GTATCC 2 cut(s) 660, 783
BsuRI GGCC 1 cut(s) 262
BtsCI GGATG 3 cut(s) 537, 607, 811
BtsIMutI CAGTG 2 cut(s) 426, 1027
CaiI CAGNNNCTG 2 cut(s) 131, 197
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 2 cut(s) 284, 478
CviAII CATG 7 cut(s) 178, 406, 458, 469, 481, 731, 1049
CviQI GTAC 2 cut(s) 284, 478
DdeI CTNAG 6 cut(s) 69, 198, 227, 486, 495, 987
DpnI GATC 1 cut(s) 813
DpnII GATC 1 cut(s) 811
Eam1104I CTCTTC 3 cut(s) 285, 546, 576
EarI CTCTTC 3 cut(s) 285, 546, 576
Ecl136II GAGCTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 130, 430
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 7 cut(s) 181, 409, 461, 472, 484, 734, 1052
FaqI GGGAC 1 cut(s) 540
FatI CATG 7 cut(s) 177, 405, 457, 468, 480, 730, 1048
Fnu4HI GCNGC 4 cut(s) 87, 192, 195, 1011
FokI GGATG 3 cut(s) 524, 614, 818
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 4 cut(s) 87, 192, 195, 1011
FspBI CTAG 3 cut(s) 287, 326, 645
GluI GCNGC 4 cut(s) 87, 192, 195, 1011
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 1 cut(s) 262
Hin1II CATG 7 cut(s) 181, 409, 461, 472, 484, 734, 1052
HindIII AAGCTT 2 cut(s) 265, 397
HinfI GANTC 3 cut(s) 181, 454, 1017
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 4 cut(s) 72, 379, 487, 1041
Hpy188III TCNNGA 2 cut(s) 126, 862
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 1 cut(s) 859
HpyCH4III ACNGT 3 cut(s) 283, 421, 771
HpyCH4V TGCA 7 cut(s) 89, 191, 235, 608, 767, 887, 1013
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 6 cut(s) 69, 198, 227, 486, 495, 987
Hsp92II CATG 7 cut(s) 181, 409, 461, 472, 484, 734, 1052
Kzo9I GATC 1 cut(s) 811
LmnI GCTCC 3 cut(s) 134, 361, 435
Lsp1109I GCAGC 4 cut(s) 73, 203, 206, 997
MaeI CTAG 3 cut(s) 287, 326, 645
MaeIII GTNAC 2 cut(s) 64, 931
MalI GATC 1 cut(s) 813
MboI GATC 1 cut(s) 811
MboII GAAGA 6 cut(s) 97, 302, 350, 563, 593, 1042
MfeI CAATTG 3 cut(s) 506, 627, 981
MhlI GDGCHC 1 cut(s) 139
MlyI GAGTC 1 cut(s) 448
MnlI CCTC 4 cut(s) 47, 547, 577, 605
MseI TTAA 8 cut(s) 218, 269, 305, 513, 560, 669, 830, 1080
MslI CAYNNNNRTG 4 cut(s) 71, 735, 947, 950
MspA1I CMGCKG 1 cut(s) 197
MspR9I CCNGG 2 cut(s) 132, 432
MunI CAATTG 3 cut(s) 506, 627, 981
Mva1269I GAATGC 2 cut(s) 235, 438
MvaI CCWGG 2 cut(s) 132, 432
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeII GATC 1 cut(s) 811
NlaIII CATG 7 cut(s) 181, 409, 461, 472, 484, 734, 1052
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 64, 931
NspI RCATGY 2 cut(s) 484, 734
PciI ACATGT 2 cut(s) 480, 730
PctI GAATGC 2 cut(s) 235, 438
PfeI GAWTC 2 cut(s) 181, 1017
PkrI GCNGC 4 cut(s) 88, 193, 196, 1012
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 128
PscI ACATGT 2 cut(s) 480, 730
Psp124BI GAGCTC 1 cut(s) 139
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 2 cut(s) 130, 430
PspGI CCWGG 2 cut(s) 130, 430
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 1 cut(s) 128
PspPPI RGGWCCY 1 cut(s) 128
PstI CTGCAG 1 cut(s) 1015
PstNI CAGNNNCTG 2 cut(s) 131, 197
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 2 cut(s) 285, 479
RsaNI GTAC 2 cut(s) 284, 478
RseI CAYNNNNRTG 4 cut(s) 71, 735, 947, 950
SacI GAGCTC 1 cut(s) 139
SaqAI TTAA 8 cut(s) 218, 269, 305, 513, 560, 669, 830, 1080
SatI GCNGC 4 cut(s) 87, 192, 195, 1011
Sau3AI GATC 1 cut(s) 811
Sau96I GGNCC 1 cut(s) 128
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 2 cut(s) 132, 432
SduI GDGCHC 1 cut(s) 139
SfcI CTRYAG 1 cut(s) 1011
SinI GGWCC 1 cut(s) 128
SmiMI CAYNNNNRTG 4 cut(s) 71, 735, 947, 950
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
SsiI CCGC 2 cut(s) 787, 912
SspI AATATT 2 cut(s) 216, 828
SspMI CTAG 3 cut(s) 287, 326, 645
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 2 cut(s) 130, 430
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 3 cut(s) 283, 421, 771
TaqI TCGA 4 cut(s) 93, 149, 675, 1004
TatI WGTACW 2 cut(s) 283, 477
TfiI GAWTC 2 cut(s) 181, 1017
Tru1I TTAA 8 cut(s) 218, 269, 305, 513, 560, 669, 830, 1080
Tru9I TTAA 8 cut(s) 218, 269, 305, 513, 560, 669, 830, 1080
TscAI CASTG 2 cut(s) 426, 1027
TseFI GTSAC 2 cut(s) 64, 931
TseI GCWGC 4 cut(s) 86, 191, 194, 1010
Tsp45I GTSAC 2 cut(s) 64, 931
TspDTI ATGAA 4 cut(s) 194, 351, 785, 1043
TspRI CASTG 2 cut(s) 426, 1027
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 2 cut(s) 46, 202
XceI RCATGY 2 cut(s) 484, 734
XspI CTAG 3 cut(s) 287, 326, 645
ZrmI AGTACT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.