Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 432 |
| AclI | AACGTT | 2 cut(s) | 684, 832 |
| AcsI | RAATTY | 4 cut(s) | 46, 168, 202, 878 |
| AfaI | GTAC | 2 cut(s) | 476, 789 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 297 |
| AjnI | CCWGG | 1 cut(s) | 427 |
| AluBI | AGCT | 9 cut(s) | 21, 86, 97, 137, 197, 264, 353, 396, 1007 |
| AluI | AGCT | 9 cut(s) | 21, 86, 97, 137, 197, 264, 353, 396, 1007 |
| Alw21I | GWGCWC | 2 cut(s) | 99, 139 |
| Alw26I | GTCTC | 2 cut(s) | 284, 305 |
| AoxI | GGCC | 2 cut(s) | 257, 523 |
| ApeKI | GCWGC | 5 cut(s) | 38, 86, 194, 197, 1007 |
| ApoI | RAATTY | 4 cut(s) | 46, 168, 202, 878 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 752, 784 |
| AspS9I | GGNCC | 1 cut(s) | 128 |
| AsuC2I | CCSGG | 2 cut(s) | 132, 960 |
| AsuHPI | GGTGA | 2 cut(s) | 400, 623 |
| AvaII | GGWCC | 1 cut(s) | 128 |
| BanI | GGYRCC | 1 cut(s) | 432 |
| BanII | GRGCYC | 2 cut(s) | 99, 139 |
| BbsI | GAAGAC | 1 cut(s) | 97 |
| Bbv12I | GWGCWC | 2 cut(s) | 99, 139 |
| BbvI | GCAGC | 5 cut(s) | 25, 73, 184, 206, 994 |
| BccI | CCATC | 5 cut(s) | 332, 542, 589, 608, 943 |
| BciT130I | CCWGG | 1 cut(s) | 429 |
| BclI | TGATCA | 1 cut(s) | 808 |
| BcnI | CCSGG | 2 cut(s) | 132, 960 |
| BcoDI | GTCTC | 2 cut(s) | 284, 305 |
| BfaI | CTAG | 4 cut(s) | 59, 323, 642, 899 |
| BfmI | CTRYAG | 1 cut(s) | 1008 |
| BisI | GCNGC | 5 cut(s) | 39, 87, 195, 198, 1008 |
| BlsI | GCNGC | 5 cut(s) | 40, 88, 196, 199, 1009 |
| BmcAI | AGTACT | 1 cut(s) | 476 |
| Bme1390I | CCNGG | 3 cut(s) | 132, 429, 960 |
| Bme18I | GGWCC | 1 cut(s) | 128 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 297 |
| BmgT120I | GGNCC | 1 cut(s) | 128 |
| BmiI | GGNNCC | 2 cut(s) | 130, 434 |
| BmrFI | CCNGG | 3 cut(s) | 132, 429, 960 |
| BpiI | GAAGAC | 1 cut(s) | 97 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 126, 158 |
| BpmI | CTGGAG | 1 cut(s) | 1082 |
| Bpu10I | CCTNAGC | 2 cut(s) | 349, 497 |
| BpuEI | CTTGAG | 2 cut(s) | 250, 678 |
| BpuMI | CCSGG | 2 cut(s) | 132, 960 |
| BsaJI | CCNNGG | 1 cut(s) | 428 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 45, 75 |
| Bse3DI | GCAATG | 1 cut(s) | 114 |
| BseBI | CCWGG | 1 cut(s) | 429 |
| BseDI | CCNNGG | 1 cut(s) | 428 |
| BseGI | GGATG | 2 cut(s) | 534, 604 |
| BseMI | GCAATG | 1 cut(s) | 114 |
| BseMII | CTCAG | 4 cut(s) | 36, 168, 474, 975 |
| BseRI | GAGGAG | 1 cut(s) | 621 |
| BseXI | GCAGC | 5 cut(s) | 25, 73, 184, 206, 994 |
| BshFI | GGCC | 2 cut(s) | 259, 525 |
| BshNI | GGYRCC | 1 cut(s) | 432 |
| BsiHKAI | GWGCWC | 2 cut(s) | 99, 139 |
| BsiSI | CCGG | 2 cut(s) | 132, 960 |
| BsmAI | GTCTC | 2 cut(s) | 284, 305 |
| BsnI | GGCC | 2 cut(s) | 259, 525 |
| Bsp1286I | GDGCHC | 2 cut(s) | 99, 139 |
| Bsp143I | GATC | 1 cut(s) | 808 |
| BspANI | GGCC | 2 cut(s) | 259, 525 |
| BspCNI | CTCAG | 4 cut(s) | 35, 169, 475, 976 |
| BspHI | TCATGA | 1 cut(s) | 945 |
| BspLI | GGNNCC | 2 cut(s) | 130, 434 |
| BspMAI | CTGCAG | 1 cut(s) | 1012 |
| BspT107I | GGYRCC | 1 cut(s) | 432 |
| BsrDI | GCAATG | 1 cut(s) | 114 |
| BssECI | CCNNGG | 1 cut(s) | 428 |
| BssMI | GATC | 1 cut(s) | 808 |
| Bst2UI | CCWGG | 1 cut(s) | 429 |
| Bst4CI | ACNGT | 2 cut(s) | 418, 768 |
| Bst6I | CTCTTC | 6 cut(s) | 282, 543, 573, 582, 906, 961 |
| BstC8I | GCNNGC | 1 cut(s) | 1012 |
| BstDEI | CTNAG | 8 cut(s) | 22, 177, 218, 227, 349, 483, 497, 984 |
| BstF5I | GGATG | 2 cut(s) | 534, 604 |
| BstKTI | GATC | 1 cut(s) | 811 |
| BstMAI | GTCTC | 2 cut(s) | 284, 305 |
| BstMBI | GATC | 1 cut(s) | 808 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 83 |
| BstNI | CCWGG | 1 cut(s) | 429 |
| BstSCI | CCNGG | 3 cut(s) | 130, 427, 958 |
| BstSFI | CTRYAG | 1 cut(s) | 1008 |
| BstV1I | GCAGC | 5 cut(s) | 25, 73, 184, 206, 994 |
| BstV2I | GAAGAC | 1 cut(s) | 97 |
| BsuRI | GGCC | 2 cut(s) | 259, 525 |
| BtsCI | GGATG | 2 cut(s) | 534, 604 |
| BtsIMutI | CAGTG | 2 cut(s) | 423, 818 |
| Cac8I | GCNNGC | 1 cut(s) | 1012 |
| CciI | TCATGA | 1 cut(s) | 945 |
| Cfr13I | GGNCC | 1 cut(s) | 128 |
| Csp6I | GTAC | 2 cut(s) | 475, 788 |
| CviAII | CATG | 4 cut(s) | 385, 403, 659, 946 |
| CviQI | GTAC | 2 cut(s) | 475, 788 |
| DdeI | CTNAG | 8 cut(s) | 22, 177, 218, 227, 349, 483, 497, 984 |
| DpnI | GATC | 1 cut(s) | 810 |
| DpnII | GATC | 1 cut(s) | 808 |
| DriI | GACNNNNNGTC | 1 cut(s) | 297 |
| Eam1104I | CTCTTC | 6 cut(s) | 282, 543, 573, 582, 906, 961 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 297 |
| EarI | CTCTTC | 6 cut(s) | 282, 543, 573, 582, 906, 961 |
| Ecl136II | GAGCTC | 2 cut(s) | 97, 137 |
| Eco24I | GRGCYC | 2 cut(s) | 99, 139 |
| Eco32I | GATATC | 1 cut(s) | 647 |
| Eco47I | GGWCC | 1 cut(s) | 128 |
| Eco53kI | GAGCTC | 2 cut(s) | 97, 137 |
| EcoICRI | GAGCTC | 2 cut(s) | 97, 137 |
| EcoO109I | RGGNCCY | 1 cut(s) | 128 |
| EcoRII | CCWGG | 1 cut(s) | 427 |
| EcoRV | GATATC | 1 cut(s) | 647 |
| EcoT38I | GRGCYC | 2 cut(s) | 99, 139 |
| FaeI | CATG | 4 cut(s) | 388, 406, 662, 949 |
| FatI | CATG | 4 cut(s) | 384, 402, 658, 945 |
| FbaI | TGATCA | 1 cut(s) | 808 |
| Fnu4HI | GCNGC | 5 cut(s) | 39, 87, 195, 198, 1008 |
| FokI | GGATG | 2 cut(s) | 521, 611 |
| FriOI | GRGCYC | 2 cut(s) | 99, 139 |
| Fsp4HI | GCNGC | 5 cut(s) | 39, 87, 195, 198, 1008 |
| FspBI | CTAG | 4 cut(s) | 59, 323, 642, 899 |
| GluI | GCNGC | 5 cut(s) | 39, 87, 195, 198, 1008 |
| GsuI | CTGGAG | 1 cut(s) | 1082 |
| HaeIII | GGCC | 2 cut(s) | 259, 525 |
| HapII | CCGG | 2 cut(s) | 132, 960 |
| Hin1II | CATG | 4 cut(s) | 388, 406, 662, 949 |
| HindIII | AAGCTT | 1 cut(s) | 394 |
| HinfI | GANTC | 2 cut(s) | 181, 313 |
| HpaII | CCGG | 2 cut(s) | 132, 960 |
| HphI | GGTGA | 2 cut(s) | 400, 623 |
| Hpy166II | GTNNAC | 2 cut(s) | 235, 565 |
| Hpy188I | TCNGA | 4 cut(s) | 25, 81, 376, 484 |
| Hpy188III | TCNNGA | 4 cut(s) | 126, 642, 946, 1061 |
| Hpy8I | GTNNAC | 2 cut(s) | 235, 565 |
| HpyAV | CCTTC | 2 cut(s) | 446, 856 |
| HpyCH4III | ACNGT | 2 cut(s) | 418, 768 |
| HpyCH4IV | ACGT | 2 cut(s) | 684, 832 |
| HpyCH4V | TGCA | 4 cut(s) | 89, 283, 884, 1010 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 83 |
| HpyF3I | CTNAG | 8 cut(s) | 22, 177, 218, 227, 349, 483, 497, 984 |
| HpySE526I | ACGT | 2 cut(s) | 684, 832 |
| Hsp92II | CATG | 4 cut(s) | 388, 406, 662, 949 |
| Ksp22I | TGATCA | 1 cut(s) | 808 |
| Kzo9I | GATC | 1 cut(s) | 808 |
| LmnI | GCTCC | 2 cut(s) | 134, 358 |
| Lsp1109I | GCAGC | 5 cut(s) | 25, 73, 184, 206, 994 |
| MaeI | CTAG | 4 cut(s) | 59, 323, 642, 899 |
| MaeII | ACGT | 2 cut(s) | 684, 832 |
| MaeIII | GTNAC | 1 cut(s) | 64 |
| MalI | GATC | 1 cut(s) | 810 |
| MboI | GATC | 1 cut(s) | 808 |
| MboII | GAAGA | 9 cut(s) | 97, 299, 347, 363, 560, 590, 599, 923, 978 |
| MfeI | CAATTG | 3 cut(s) | 90, 503, 978 |
| MhlI | GDGCHC | 2 cut(s) | 99, 139 |
| MlyI | GAGTC | 1 cut(s) | 307 |
| MmeI | TCCRAC | 1 cut(s) | 583 |
| MnlI | CCTC | 8 cut(s) | 47, 544, 548, 574, 599, 602, 835, 907 |
| MseI | TTAA | 3 cut(s) | 510, 557, 666 |
| MslI | CAYNNNNRTG | 2 cut(s) | 71, 657 |
| MspA1I | CMGCKG | 1 cut(s) | 197 |
| MspI | CCGG | 2 cut(s) | 132, 960 |
| MspR9I | CCNGG | 3 cut(s) | 132, 429, 960 |
| MunI | CAATTG | 3 cut(s) | 90, 503, 978 |
| MvaI | CCWGG | 1 cut(s) | 429 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 83 |
| NciI | CCSGG | 2 cut(s) | 132, 960 |
| NdeII | GATC | 1 cut(s) | 808 |
| NlaIII | CATG | 4 cut(s) | 388, 406, 662, 949 |
| NlaIV | GGNNCC | 2 cut(s) | 130, 434 |
| NmuCI | GTSAC | 1 cut(s) | 64 |
| PagI | TCATGA | 1 cut(s) | 945 |
| PfeI | GAWTC | 1 cut(s) | 181 |
| PkrI | GCNGC | 5 cut(s) | 40, 88, 196, 199, 1009 |
| PleI | GAGTC | 1 cut(s) | 307 |
| PpsI | GAGTC | 1 cut(s) | 307 |
| PpuMI | RGGWCCY | 1 cut(s) | 128 |
| Psp124BI | GAGCTC | 2 cut(s) | 99, 139 |
| Psp1406I | AACGTT | 2 cut(s) | 684, 832 |
| Psp5II | RGGWCCY | 1 cut(s) | 128 |
| Psp6I | CCWGG | 1 cut(s) | 427 |
| PspGI | CCWGG | 1 cut(s) | 427 |
| PspN4I | GGNNCC | 2 cut(s) | 130, 434 |
| PspPI | GGNCC | 1 cut(s) | 128 |
| PspPPI | RGGWCCY | 1 cut(s) | 128 |
| PstI | CTGCAG | 1 cut(s) | 1012 |
| PvuII | CAGCTG | 1 cut(s) | 197 |
| RsaI | GTAC | 2 cut(s) | 476, 789 |
| RsaNI | GTAC | 2 cut(s) | 475, 788 |
| RseI | CAYNNNNRTG | 2 cut(s) | 71, 657 |
| SacI | GAGCTC | 2 cut(s) | 99, 139 |
| SaqAI | TTAA | 3 cut(s) | 510, 557, 666 |
| SatI | GCNGC | 5 cut(s) | 39, 87, 195, 198, 1008 |
| Sau3AI | GATC | 1 cut(s) | 808 |
| Sau96I | GGNCC | 1 cut(s) | 128 |
| ScaI | AGTACT | 1 cut(s) | 476 |
| SchI | GAGTC | 1 cut(s) | 307 |
| ScrFI | CCNGG | 3 cut(s) | 132, 429, 960 |
| SduI | GDGCHC | 2 cut(s) | 99, 139 |
| SfcI | CTRYAG | 1 cut(s) | 1008 |
| SinI | GGWCC | 1 cut(s) | 128 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 71, 657 |
| SmlI | CTYRAG | 2 cut(s) | 265, 693 |
| SmoI | CTYRAG | 2 cut(s) | 265, 693 |
| SspI | AATATT | 1 cut(s) | 825 |
| SspMI | CTAG | 4 cut(s) | 59, 323, 642, 899 |
| SstI | GAGCTC | 2 cut(s) | 99, 139 |
| StyD4I | CCNGG | 3 cut(s) | 130, 427, 958 |
| TaaI | ACNGT | 2 cut(s) | 418, 768 |
| TaiI | ACGT | 2 cut(s) | 687, 835 |
| TaqI | TCGA | 2 cut(s) | 149, 540 |
| TatI | WGTACW | 1 cut(s) | 474 |
| TfiI | GAWTC | 1 cut(s) | 181 |
| Tru1I | TTAA | 3 cut(s) | 510, 557, 666 |
| Tru9I | TTAA | 3 cut(s) | 510, 557, 666 |
| TscAI | CASTG | 2 cut(s) | 423, 818 |
| TseFI | GTSAC | 1 cut(s) | 64 |
| TseI | GCWGC | 5 cut(s) | 38, 86, 194, 197, 1007 |
| Tsp45I | GTSAC | 1 cut(s) | 64 |
| TspDTI | ATGAA | 3 cut(s) | 348, 647, 782 |
| TspGWI | ACGGA | 1 cut(s) | 923 |
| TspRI | CASTG | 2 cut(s) | 423, 818 |
| VpaK11BI | GGWCC | 1 cut(s) | 128 |
| XapI | RAATTY | 4 cut(s) | 46, 168, 202, 878 |
| XbaI | TCTAGA | 1 cut(s) | 641 |
| XspI | CTAG | 4 cut(s) | 59, 323, 642, 899 |
| ZrmI | AGTACT | 1 cut(s) | 476 |