Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 173 |
| Acc36I | ACCTGC | 1 cut(s) | 173 |
| AciI | CCGC | 2 cut(s) | 923, 963 |
| AclWI | GGATC | 2 cut(s) | 591, 982 |
| AcoI | YGGCCR | 1 cut(s) | 885 |
| AcsI | RAATTY | 3 cut(s) | 43, 520, 872 |
| AcuI | CTGAAG | 1 cut(s) | 9 |
| AfaI | GTAC | 2 cut(s) | 404, 669 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 679, 737 |
| AflII | CTTAAG | 1 cut(s) | 956 |
| AflIII | ACRYGT | 1 cut(s) | 468 |
| AgsI | TTSAA | 4 cut(s) | 59, 97, 416, 908 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 469 |
| AhlI | ACTAGT | 1 cut(s) | 778 |
| AjnI | CCWGG | 1 cut(s) | 882 |
| AluBI | AGCT | 7 cut(s) | 29, 74, 85, 125, 387, 487, 590 |
| AluI | AGCT | 7 cut(s) | 29, 74, 85, 125, 387, 487, 590 |
| Alw21I | GWGCWC | 3 cut(s) | 87, 127, 762 |
| AlwI | GGATC | 2 cut(s) | 591, 982 |
| AoxI | GGCC | 1 cut(s) | 885 |
| ApeKI | GCWGC | 1 cut(s) | 74 |
| ApoI | RAATTY | 3 cut(s) | 43, 520, 872 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 632, 664 |
| AspS9I | GGNCC | 2 cut(s) | 116, 966 |
| AsuC2I | CCSGG | 1 cut(s) | 120 |
| AsuHPI | GGTGA | 5 cut(s) | 43, 119, 197, 520, 589 |
| AsuII | TTCGAA | 1 cut(s) | 570 |
| AvaII | GGWCC | 2 cut(s) | 116, 966 |
| BalI | TGGCCA | 1 cut(s) | 887 |
| BanII | GRGCYC | 3 cut(s) | 87, 127, 365 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 374, 406 |
| BauI | CACGAG | 1 cut(s) | 930 |
| Bbv12I | GWGCWC | 3 cut(s) | 87, 127, 762 |
| BbvI | GCAGC | 1 cut(s) | 61 |
| BccI | CCATC | 4 cut(s) | 56, 323, 505, 587 |
| BciT130I | CCWGG | 1 cut(s) | 884 |
| BclI | TGATCA | 1 cut(s) | 538 |
| BcnI | CCSGG | 1 cut(s) | 120 |
| BcuI | ACTAGT | 1 cut(s) | 778 |
| BfaI | CTAG | 2 cut(s) | 314, 779 |
| BfmI | CTRYAG | 1 cut(s) | 75 |
| BfrI | CTTAAG | 1 cut(s) | 956 |
| BfuAI | ACCTGC | 1 cut(s) | 173 |
| BglII | AGATCT | 1 cut(s) | 915 |
| BisI | GCNGC | 1 cut(s) | 75 |
| BlsI | GCNGC | 1 cut(s) | 76 |
| Bme1390I | CCNGG | 2 cut(s) | 120, 884 |
| Bme18I | GGWCC | 2 cut(s) | 116, 966 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 469 |
| BmgT120I | GGNCC | 2 cut(s) | 116, 966 |
| BmiI | GGNNCC | 3 cut(s) | 118, 364, 967 |
| BmrFI | CCNGG | 2 cut(s) | 120, 884 |
| Bpu10I | CCTNAGC | 1 cut(s) | 483 |
| Bpu14I | TTCGAA | 1 cut(s) | 570 |
| BpuEI | CTTGAG | 1 cut(s) | 383 |
| BpuMI | CCSGG | 1 cut(s) | 120 |
| BsaJI | CCNNGG | 2 cut(s) | 445, 969 |
| BsaWI | WCCGGW | 1 cut(s) | 418 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 679, 737 |
| Bse3DI | GCAATG | 1 cut(s) | 102 |
| BseBI | CCWGG | 1 cut(s) | 884 |
| BseDI | CCNNGG | 2 cut(s) | 445, 969 |
| BseGI | GGATG | 3 cut(s) | 239, 602, 688 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 679, 737 |
| BseMI | GCAATG | 1 cut(s) | 102 |
| BseMII | CTCAG | 2 cut(s) | 59, 474 |
| BseXI | GCAGC | 1 cut(s) | 61 |
| BshFI | GGCC | 1 cut(s) | 887 |
| BsiHKAI | GWGCWC | 3 cut(s) | 87, 127, 762 |
| BsiSI | CCGG | 2 cut(s) | 120, 419 |
| BslFI | GGGAC | 4 cut(s) | 102, 302, 528, 979 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 679, 737 |
| BsmFI | GGGAC | 4 cut(s) | 102, 302, 528, 979 |
| BsmI | GAATGC | 1 cut(s) | 207 |
| BsnI | GGCC | 1 cut(s) | 887 |
| Bsp119I | TTCGAA | 1 cut(s) | 570 |
| Bsp1286I | GDGCHC | 4 cut(s) | 87, 127, 365, 762 |
| Bsp143I | GATC | 4 cut(s) | 538, 583, 915, 974 |
| Bsp19I | CCATGG | 1 cut(s) | 445 |
| BspACI | CCGC | 2 cut(s) | 923, 963 |
| BspANI | GGCC | 1 cut(s) | 887 |
| BspCNI | CTCAG | 2 cut(s) | 60, 475 |
| BspHI | TCATGA | 1 cut(s) | 828 |
| BspLI | GGNNCC | 3 cut(s) | 118, 364, 967 |
| BspMAI | CTGCAG | 1 cut(s) | 79 |
| BspMI | ACCTGC | 1 cut(s) | 173 |
| BspPI | GGATC | 2 cut(s) | 591, 982 |
| BspQI | GCTCTTC | 1 cut(s) | 377 |
| BspT104I | TTCGAA | 1 cut(s) | 570 |
| BspTI | CTTAAG | 1 cut(s) | 956 |
| BsrDI | GCAATG | 1 cut(s) | 102 |
| BssECI | CCNNGG | 2 cut(s) | 445, 969 |
| BssMI | GATC | 4 cut(s) | 538, 583, 915, 974 |
| BssSI | CACGAG | 1 cut(s) | 930 |
| BssT1I | CCWWGG | 1 cut(s) | 445 |
| Bst2BI | CACGAG | 1 cut(s) | 930 |
| Bst2UI | CCWGG | 1 cut(s) | 884 |
| Bst4CI | ACNGT | 3 cut(s) | 695, 716, 970 |
| Bst6I | CTCTTC | 2 cut(s) | 377, 567 |
| BstAFI | CTTAAG | 1 cut(s) | 956 |
| BstBI | TTCGAA | 1 cut(s) | 570 |
| BstDEI | CTNAG | 2 cut(s) | 68, 483 |
| BstDSI | CCRYGG | 2 cut(s) | 445, 969 |
| BstF5I | GGATG | 3 cut(s) | 239, 602, 688 |
| BstKTI | GATC | 4 cut(s) | 541, 586, 918, 977 |
| BstMBI | GATC | 4 cut(s) | 538, 583, 915, 974 |
| BstNI | CCWGG | 1 cut(s) | 884 |
| BstNSI | RCATGY | 3 cut(s) | 397, 472, 611 |
| BstSCI | CCNGG | 2 cut(s) | 118, 882 |
| BstSFI | CTRYAG | 1 cut(s) | 75 |
| BstV1I | GCAGC | 1 cut(s) | 61 |
| BstX2I | RGATCY | 1 cut(s) | 915 |
| BstYI | RGATCY | 1 cut(s) | 915 |
| BsuRI | GGCC | 1 cut(s) | 887 |
| BtgI | CCRYGG | 2 cut(s) | 445, 969 |
| BtsCI | GGATG | 3 cut(s) | 239, 602, 688 |
| BtsIMutI | CAGTG | 1 cut(s) | 691 |
| BveI | ACCTGC | 1 cut(s) | 173 |
| CciI | TCATGA | 1 cut(s) | 828 |
| Cfr13I | GGNCC | 2 cut(s) | 116, 966 |
| CseI | GACGC | 1 cut(s) | 652 |
| Csp6I | GTAC | 2 cut(s) | 403, 668 |
| CviAII | CATG | 9 cut(s) | 394, 446, 469, 608, 655, 764, 829, 838, 941 |
| CviQI | GTAC | 2 cut(s) | 403, 668 |
| DdeI | CTNAG | 2 cut(s) | 68, 483 |
| DpnI | GATC | 4 cut(s) | 540, 585, 917, 976 |
| DpnII | GATC | 4 cut(s) | 538, 583, 915, 974 |
| DriI | GACNNNNNGTC | 1 cut(s) | 469 |
| EaeI | YGGCCR | 1 cut(s) | 885 |
| Eam1104I | CTCTTC | 2 cut(s) | 377, 567 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 469 |
| EarI | CTCTTC | 2 cut(s) | 377, 567 |
| Ecl136II | GAGCTC | 2 cut(s) | 85, 125 |
| Eco130I | CCWWGG | 1 cut(s) | 445 |
| Eco24I | GRGCYC | 3 cut(s) | 87, 127, 365 |
| Eco32I | GATATC | 1 cut(s) | 880 |
| Eco47I | GGWCC | 2 cut(s) | 116, 966 |
| Eco53kI | GAGCTC | 2 cut(s) | 85, 125 |
| Eco57I | CTGAAG | 1 cut(s) | 9 |
| EcoICRI | GAGCTC | 2 cut(s) | 85, 125 |
| EcoO109I | RGGNCCY | 1 cut(s) | 116 |
| EcoRII | CCWGG | 1 cut(s) | 882 |
| EcoRV | GATATC | 1 cut(s) | 880 |
| EcoT14I | CCWWGG | 1 cut(s) | 445 |
| EcoT38I | GRGCYC | 3 cut(s) | 87, 127, 365 |
| ErhI | CCWWGG | 1 cut(s) | 445 |
| FaeI | CATG | 9 cut(s) | 397, 449, 472, 611, 658, 767, 832, 841, 944 |
| FaqI | GGGAC | 4 cut(s) | 102, 302, 528, 979 |
| FatI | CATG | 9 cut(s) | 393, 445, 468, 607, 654, 763, 828, 837, 940 |
| FauI | CCCGC | 2 cut(s) | 930, 956 |
| FbaI | TGATCA | 1 cut(s) | 538 |
| Fnu4HI | GCNGC | 1 cut(s) | 75 |
| FokI | GGATG | 3 cut(s) | 226, 609, 695 |
| FriOI | GRGCYC | 3 cut(s) | 87, 127, 365 |
| Fsp4HI | GCNGC | 1 cut(s) | 75 |
| FspBI | CTAG | 2 cut(s) | 314, 779 |
| GluI | GCNGC | 1 cut(s) | 75 |
| HaeIII | GGCC | 1 cut(s) | 887 |
| HapII | CCGG | 2 cut(s) | 120, 419 |
| HgaI | GACGC | 1 cut(s) | 652 |
| Hin1II | CATG | 9 cut(s) | 397, 449, 472, 611, 658, 767, 832, 841, 944 |
| HinfI | GANTC | 3 cut(s) | 64, 454, 937 |
| HpaII | CCGG | 2 cut(s) | 120, 419 |
| HphI | GGTGA | 5 cut(s) | 43, 119, 197, 520, 589 |
| Hpy166II | GTNNAC | 2 cut(s) | 237, 559 |
| Hpy188I | TCNGA | 2 cut(s) | 69, 367 |
| Hpy188III | TCNNGA | 2 cut(s) | 319, 829 |
| Hpy8I | GTNNAC | 2 cut(s) | 237, 559 |
| HpyAV | CCTTC | 2 cut(s) | 574, 736 |
| HpyCH4III | ACNGT | 3 cut(s) | 695, 716, 970 |
| HpyCH4V | TGCA | 1 cut(s) | 77 |
| HpyF3I | CTNAG | 2 cut(s) | 68, 483 |
| Hsp92II | CATG | 9 cut(s) | 397, 449, 472, 611, 658, 767, 832, 841, 944 |
| Ksp22I | TGATCA | 1 cut(s) | 538 |
| Kzo9I | GATC | 4 cut(s) | 538, 583, 915, 974 |
| LguI | GCTCTTC | 1 cut(s) | 377 |
| LmnI | GCTCC | 5 cut(s) | 122, 196, 368, 604, 856 |
| Lsp1109I | GCAGC | 1 cut(s) | 61 |
| MaeI | CTAG | 2 cut(s) | 314, 779 |
| MaeIII | GTNAC | 1 cut(s) | 185 |
| MalI | GATC | 4 cut(s) | 540, 585, 917, 976 |
| MboI | GATC | 4 cut(s) | 538, 583, 915, 974 |
| MboII | GAAGA | 4 cut(s) | 338, 394, 584, 910 |
| MfeI | CAATTG | 1 cut(s) | 494 |
| MflI | RGATCY | 1 cut(s) | 915 |
| MhlI | GDGCHC | 4 cut(s) | 87, 127, 365, 762 |
| MlsI | TGGCCA | 1 cut(s) | 887 |
| MluCI | AATT | 7 cut(s) | 43, 144, 190, 494, 520, 676, 872 |
| MluNI | TGGCCA | 1 cut(s) | 887 |
| MlyI | GAGTC | 3 cut(s) | 58, 463, 946 |
| MmeI | TCCRAC | 2 cut(s) | 197, 960 |
| MnlI | CCTC | 4 cut(s) | 547, 568, 715, 939 |
| Mox20I | TGGCCA | 1 cut(s) | 887 |
| MscI | TGGCCA | 1 cut(s) | 887 |
| MseI | TTAA | 2 cut(s) | 632, 957 |
| MslI | CAYNNNNRTG | 4 cut(s) | 59, 612, 659, 827 |
| Msp20I | TGGCCA | 1 cut(s) | 887 |
| MspCI | CTTAAG | 1 cut(s) | 956 |
| MspI | CCGG | 2 cut(s) | 120, 419 |
| MspR9I | CCNGG | 2 cut(s) | 120, 884 |
| MunI | CAATTG | 1 cut(s) | 494 |
| Mva1269I | GAATGC | 1 cut(s) | 207 |
| MvaI | CCWGG | 1 cut(s) | 884 |
| NciI | CCSGG | 1 cut(s) | 120 |
| NcoI | CCATGG | 1 cut(s) | 445 |
| NdeII | GATC | 4 cut(s) | 538, 583, 915, 974 |
| NlaIII | CATG | 9 cut(s) | 397, 449, 472, 611, 658, 767, 832, 841, 944 |
| NlaIV | GGNNCC | 3 cut(s) | 118, 364, 967 |
| NmuCI | GTSAC | 1 cut(s) | 185 |
| NspI | RCATGY | 3 cut(s) | 397, 472, 611 |
| NspV | TTCGAA | 1 cut(s) | 570 |
| PagI | TCATGA | 1 cut(s) | 828 |
| PaqCI | CACCTGC | 1 cut(s) | 173 |
| PciI | ACATGT | 1 cut(s) | 468 |
| PciSI | GCTCTTC | 1 cut(s) | 377 |
| PctI | GAATGC | 1 cut(s) | 207 |
| PflFI | GACNNNGTC | 1 cut(s) | 646 |
| PfoI | TCCNGGA | 1 cut(s) | 118 |
| PkrI | GCNGC | 1 cut(s) | 76 |
| PleI | GAGTC | 3 cut(s) | 58, 462, 945 |
| PpsI | GAGTC | 3 cut(s) | 58, 462, 945 |
| PpuMI | RGGWCCY | 1 cut(s) | 116 |
| PscI | ACATGT | 1 cut(s) | 468 |
| Psp124BI | GAGCTC | 2 cut(s) | 87, 127 |
| Psp5II | RGGWCCY | 1 cut(s) | 116 |
| Psp6I | CCWGG | 1 cut(s) | 882 |
| PspGI | CCWGG | 1 cut(s) | 882 |
| PspN4I | GGNNCC | 3 cut(s) | 118, 364, 967 |
| PspPI | GGNCC | 2 cut(s) | 116, 966 |
| PspPPI | RGGWCCY | 1 cut(s) | 116 |
| PstI | CTGCAG | 1 cut(s) | 79 |
| PsuI | RGATCY | 1 cut(s) | 915 |
| PsyI | GACNNNGTC | 1 cut(s) | 646 |
| RsaI | GTAC | 2 cut(s) | 404, 669 |
| RsaNI | GTAC | 2 cut(s) | 403, 668 |
| RseI | CAYNNNNRTG | 4 cut(s) | 59, 612, 659, 827 |
| SacI | GAGCTC | 2 cut(s) | 87, 127 |
| SapI | GCTCTTC | 1 cut(s) | 377 |
| SaqAI | TTAA | 2 cut(s) | 632, 957 |
| SatI | GCNGC | 1 cut(s) | 75 |
| Sau3AI | GATC | 4 cut(s) | 538, 583, 915, 974 |
| Sau96I | GGNCC | 2 cut(s) | 116, 966 |
| SchI | GAGTC | 3 cut(s) | 58, 463, 946 |
| ScrFI | CCNGG | 2 cut(s) | 120, 884 |
| SduI | GDGCHC | 4 cut(s) | 87, 127, 365, 762 |
| SfcI | CTRYAG | 1 cut(s) | 75 |
| SfuI | TTCGAA | 1 cut(s) | 570 |
| SinI | GGWCC | 2 cut(s) | 116, 966 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 59, 612, 659, 827 |
| SmlI | CTYRAG | 2 cut(s) | 398, 956 |
| SmoI | CTYRAG | 2 cut(s) | 398, 956 |
| SpeI | ACTAGT | 1 cut(s) | 778 |
| Sse9I | AATT | 7 cut(s) | 43, 144, 190, 494, 520, 676, 872 |
| SsiI | CCGC | 2 cut(s) | 923, 963 |
| SspMI | CTAG | 2 cut(s) | 314, 779 |
| SstI | GAGCTC | 2 cut(s) | 87, 127 |
| StyD4I | CCNGG | 2 cut(s) | 118, 882 |
| StyI | CCWWGG | 1 cut(s) | 445 |
| TaaI | ACNGT | 3 cut(s) | 695, 716, 970 |
| TaqI | TCGA | 2 cut(s) | 570, 586 |
| TasI | AATT | 7 cut(s) | 43, 144, 190, 494, 520, 676, 872 |
| TatI | WGTACW | 1 cut(s) | 402 |
| Tru1I | TTAA | 2 cut(s) | 632, 957 |
| Tru9I | TTAA | 2 cut(s) | 632, 957 |
| TscAI | CASTG | 1 cut(s) | 698 |
| TseFI | GTSAC | 1 cut(s) | 185 |
| TseI | GCWGC | 1 cut(s) | 74 |
| Tsp45I | GTSAC | 1 cut(s) | 185 |
| TspDTI | ATGAA | 5 cut(s) | 212, 339, 702, 815, 817 |
| TspGWI | ACGGA | 2 cut(s) | 232, 883 |
| TspRI | CASTG | 1 cut(s) | 698 |
| Tth111I | GACNNNGTC | 1 cut(s) | 646 |
| Vha464I | CTTAAG | 1 cut(s) | 956 |
| VpaK11BI | GGWCC | 2 cut(s) | 116, 966 |
| XapI | RAATTY | 3 cut(s) | 43, 520, 872 |
| XceI | RCATGY | 3 cut(s) | 397, 472, 611 |
| XspI | CTAG | 2 cut(s) | 314, 779 |