Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 691 |
| AcsI | RAATTY | 6 cut(s) | 7, 154, 404, 418, 712, 720 |
| AdeI | CACNNNGTG | 1 cut(s) | 373 |
| AflII | CTTAAG | 2 cut(s) | 170, 515 |
| AflIII | ACRYGT | 1 cut(s) | 432 |
| AgsI | TTSAA | 6 cut(s) | 23, 61, 466, 536, 631, 718 |
| AluBI | AGCT | 9 cut(s) | 38, 49, 89, 149, 175, 351, 472, 550, 656 |
| AluI | AGCT | 9 cut(s) | 38, 49, 89, 149, 175, 351, 472, 550, 656 |
| Alw21I | GWGCWC | 1 cut(s) | 51 |
| Alw26I | GTCTC | 2 cut(s) | 436, 443 |
| AoxI | GGCC | 2 cut(s) | 80, 253 |
| ApeKI | GCWGC | 3 cut(s) | 38, 146, 280 |
| ApoI | RAATTY | 6 cut(s) | 7, 154, 404, 418, 712, 720 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 581, 613 |
| AspS9I | GGNCC | 1 cut(s) | 80 |
| AsuHPI | GGTGA | 1 cut(s) | 581 |
| BanII | GRGCYC | 2 cut(s) | 51, 329 |
| BbsI | GAAGAC | 1 cut(s) | 49 |
| Bbv12I | GWGCWC | 1 cut(s) | 51 |
| BbvI | GCAGC | 3 cut(s) | 25, 158, 267 |
| BccI | CCATC | 2 cut(s) | 287, 566 |
| BciVI | GTATCC | 1 cut(s) | 615 |
| BclI | TGATCA | 1 cut(s) | 502 |
| BcoDI | GTCTC | 2 cut(s) | 436, 443 |
| BfaI | CTAG | 1 cut(s) | 639 |
| BfrI | CTTAAG | 2 cut(s) | 170, 515 |
| BfuI | GTATCC | 1 cut(s) | 615 |
| BisI | GCNGC | 3 cut(s) | 39, 147, 281 |
| BlsI | GCNGC | 3 cut(s) | 40, 148, 282 |
| BmgT120I | GGNCC | 1 cut(s) | 80 |
| BmsI | GCATC | 1 cut(s) | 630 |
| BpiI | GAAGAC | 1 cut(s) | 49 |
| BpuEI | CTTGAG | 3 cut(s) | 347, 398, 636 |
| BsaI | GGTCTC | 1 cut(s) | 436 |
| BsaJI | CCNNGG | 1 cut(s) | 616 |
| Bse3DI | GCAATG | 1 cut(s) | 66 |
| BseDI | CCNNGG | 1 cut(s) | 616 |
| BseGI | GGATG | 2 cut(s) | 612, 704 |
| BseMI | GCAATG | 1 cut(s) | 66 |
| BseMII | CTCAG | 1 cut(s) | 384 |
| BseXI | GCAGC | 3 cut(s) | 25, 158, 267 |
| BshFI | GGCC | 2 cut(s) | 82, 255 |
| BsiHKAI | GWGCWC | 1 cut(s) | 51 |
| BslFI | GGGAC | 1 cut(s) | 421 |
| BsmAI | GTCTC | 2 cut(s) | 436, 443 |
| BsmFI | GGGAC | 1 cut(s) | 421 |
| BsmI | GAATGC | 1 cut(s) | 18 |
| BsnI | GGCC | 2 cut(s) | 82, 255 |
| Bso31I | GGTCTC | 1 cut(s) | 436 |
| Bsp1286I | GDGCHC | 2 cut(s) | 51, 329 |
| Bsp143I | GATC | 1 cut(s) | 502 |
| Bsp19I | CCATGG | 1 cut(s) | 616 |
| BspANI | GGCC | 2 cut(s) | 82, 255 |
| BspCNI | CTCAG | 1 cut(s) | 383 |
| BspHI | TCATGA | 1 cut(s) | 474 |
| BspTI | CTTAAG | 2 cut(s) | 170, 515 |
| BspTNI | GGTCTC | 1 cut(s) | 436 |
| BsrDI | GCAATG | 1 cut(s) | 66 |
| BssECI | CCNNGG | 1 cut(s) | 616 |
| BssMI | GATC | 1 cut(s) | 502 |
| BssNAI | GTATAC | 1 cut(s) | 692 |
| BssT1I | CCWWGG | 1 cut(s) | 616 |
| Bst1107I | GTATAC | 1 cut(s) | 692 |
| BstAFI | CTTAAG | 2 cut(s) | 170, 515 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 343, 373 |
| BstC8I | GCNNGC | 1 cut(s) | 278 |
| BstDEI | CTNAG | 5 cut(s) | 32, 72, 150, 179, 370 |
| BstDSI | CCRYGG | 1 cut(s) | 616 |
| BstF5I | GGATG | 2 cut(s) | 612, 704 |
| BstKTI | GATC | 1 cut(s) | 505 |
| BstMAI | GTCTC | 2 cut(s) | 436, 443 |
| BstMBI | GATC | 1 cut(s) | 502 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 35, 343, 373 |
| BstNSI | RCATGY | 2 cut(s) | 361, 436 |
| BstV1I | GCAGC | 3 cut(s) | 25, 158, 267 |
| BstV2I | GAAGAC | 1 cut(s) | 49 |
| BstZ17I | GTATAC | 1 cut(s) | 692 |
| BsuI | GTATCC | 1 cut(s) | 615 |
| BsuRI | GGCC | 2 cut(s) | 82, 255 |
| BtgI | CCRYGG | 1 cut(s) | 616 |
| BtsCI | GGATG | 2 cut(s) | 612, 704 |
| BtsIMutI | CAGTG | 1 cut(s) | 378 |
| Cac8I | GCNNGC | 1 cut(s) | 278 |
| CciI | TCATGA | 1 cut(s) | 474 |
| Cfr13I | GGNCC | 1 cut(s) | 80 |
| CviAII | CATG | 4 cut(s) | 358, 433, 475, 617 |
| DdeI | CTNAG | 5 cut(s) | 32, 72, 150, 179, 370 |
| DpnI | GATC | 1 cut(s) | 504 |
| DpnII | GATC | 1 cut(s) | 502 |
| DraIII | CACNNNGTG | 1 cut(s) | 373 |
| Ecl136II | GAGCTC | 1 cut(s) | 49 |
| Eco130I | CCWWGG | 1 cut(s) | 616 |
| Eco24I | GRGCYC | 2 cut(s) | 51, 329 |
| Eco31I | GGTCTC | 1 cut(s) | 436 |
| Eco53kI | GAGCTC | 1 cut(s) | 49 |
| EcoICRI | GAGCTC | 1 cut(s) | 49 |
| EcoT14I | CCWWGG | 1 cut(s) | 616 |
| EcoT38I | GRGCYC | 2 cut(s) | 51, 329 |
| ErhI | CCWWGG | 1 cut(s) | 616 |
| FaeI | CATG | 4 cut(s) | 361, 436, 478, 620 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 78, 110 |
| FaqI | GGGAC | 1 cut(s) | 421 |
| FatI | CATG | 4 cut(s) | 357, 432, 474, 616 |
| FbaI | TGATCA | 1 cut(s) | 502 |
| FblI | GTMKAC | 1 cut(s) | 691 |
| Fnu4HI | GCNGC | 3 cut(s) | 39, 147, 281 |
| FokI | GGATG | 2 cut(s) | 599, 691 |
| FriOI | GRGCYC | 2 cut(s) | 51, 329 |
| Fsp4HI | GCNGC | 3 cut(s) | 39, 147, 281 |
| FspBI | CTAG | 1 cut(s) | 639 |
| GluI | GCNGC | 3 cut(s) | 39, 147, 281 |
| HaeIII | GGCC | 2 cut(s) | 82, 255 |
| Hin1II | CATG | 4 cut(s) | 361, 436, 478, 620 |
| HindIII | AAGCTT | 1 cut(s) | 173 |
| HinfI | GANTC | 1 cut(s) | 165 |
| HphI | GGTGA | 1 cut(s) | 581 |
| Hpy166II | GTNNAC | 1 cut(s) | 692 |
| Hpy188I | TCNGA | 2 cut(s) | 331, 507 |
| Hpy188III | TCNNGA | 1 cut(s) | 475 |
| Hpy8I | GTNNAC | 1 cut(s) | 692 |
| HpyAV | CCTTC | 1 cut(s) | 502 |
| HpyCH4IV | ACGT | 1 cut(s) | 339 |
| HpyCH4V | TGCA | 6 cut(s) | 18, 41, 269, 337, 346, 587 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 35, 343, 373 |
| HpyF3I | CTNAG | 5 cut(s) | 32, 72, 150, 179, 370 |
| HpySE526I | ACGT | 1 cut(s) | 339 |
| Hsp92II | CATG | 4 cut(s) | 361, 436, 478, 620 |
| Ksp22I | TGATCA | 1 cut(s) | 502 |
| Kzo9I | GATC | 1 cut(s) | 502 |
| LmnI | GCTCC | 1 cut(s) | 661 |
| LpnPI | CCDG | 6 cut(s) | 63, 96, 290, 396, 452, 459 |
| Lsp1109I | GCAGC | 3 cut(s) | 25, 158, 267 |
| LweI | GCATC | 1 cut(s) | 630 |
| MaeI | CTAG | 1 cut(s) | 639 |
| MaeII | ACGT | 1 cut(s) | 339 |
| MalI | GATC | 1 cut(s) | 504 |
| MboI | GATC | 1 cut(s) | 502 |
| MboII | GAAGA | 3 cut(s) | 49, 302, 548 |
| MhlI | GDGCHC | 2 cut(s) | 51, 329 |
| MnlI | CCTC | 5 cut(s) | 203, 214, 496, 521, 618 |
| MseI | TTAA | 2 cut(s) | 171, 516 |
| MslI | CAYNNNNRTG | 2 cut(s) | 23, 615 |
| MspCI | CTTAAG | 2 cut(s) | 170, 515 |
| Mva1269I | GAATGC | 1 cut(s) | 18 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 35, 343, 373 |
| NcoI | CCATGG | 1 cut(s) | 616 |
| NdeII | GATC | 1 cut(s) | 502 |
| NlaIII | CATG | 4 cut(s) | 361, 436, 478, 620 |
| NspI | RCATGY | 2 cut(s) | 361, 436 |
| PagI | TCATGA | 1 cut(s) | 474 |
| PciI | ACATGT | 1 cut(s) | 432 |
| PctI | GAATGC | 1 cut(s) | 18 |
| PfeI | GAWTC | 1 cut(s) | 165 |
| PkrI | GCNGC | 3 cut(s) | 40, 148, 282 |
| PscI | ACATGT | 1 cut(s) | 432 |
| Psp124BI | GAGCTC | 1 cut(s) | 51 |
| PspPI | GGNCC | 1 cut(s) | 80 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 675, 707 |
| RseI | CAYNNNNRTG | 2 cut(s) | 23, 615 |
| SacI | GAGCTC | 1 cut(s) | 51 |
| SaqAI | TTAA | 2 cut(s) | 171, 516 |
| SatI | GCNGC | 3 cut(s) | 39, 147, 281 |
| Sau3AI | GATC | 1 cut(s) | 502 |
| Sau96I | GGNCC | 1 cut(s) | 80 |
| SduI | GDGCHC | 2 cut(s) | 51, 329 |
| SfaNI | GCATC | 1 cut(s) | 630 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 23, 615 |
| SmlI | CTYRAG | 5 cut(s) | 170, 362, 377, 515, 651 |
| SmoI | CTYRAG | 5 cut(s) | 170, 362, 377, 515, 651 |
| SspMI | CTAG | 1 cut(s) | 639 |
| SstI | GAGCTC | 1 cut(s) | 51 |
| StyI | CCWWGG | 1 cut(s) | 616 |
| TaiI | ACGT | 1 cut(s) | 342 |
| TaqI | TCGA | 2 cut(s) | 51, 526 |
| TfiI | GAWTC | 1 cut(s) | 165 |
| Tru1I | TTAA | 2 cut(s) | 171, 516 |
| Tru9I | TTAA | 2 cut(s) | 171, 516 |
| TscAI | CASTG | 1 cut(s) | 378 |
| TseI | GCWGC | 3 cut(s) | 38, 146, 280 |
| TspDTI | ATGAA | 3 cut(s) | 303, 441, 447 |
| TspRI | CASTG | 1 cut(s) | 378 |
| Vha464I | CTTAAG | 2 cut(s) | 170, 515 |
| XapI | RAATTY | 6 cut(s) | 7, 154, 404, 418, 712, 720 |
| XceI | RCATGY | 2 cut(s) | 361, 436 |
| XmiI | GTMKAC | 1 cut(s) | 691 |
| XspI | CTAG | 1 cut(s) | 639 |