Rroxscaffold_1G00023690
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000656
Physical Location & Seq
Reverse (-)
29294986 .. 29297823
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00023690.1

Sequence Viewer

Length: 1092 bp
ATGGAAATCCAGAGAATTAGCTCAAAGGACTATGTATCTGCTTGGAATTTGAGTGGTCTAGTTGTCACTCAAATGGCTTTAAAAGCTGCAATCGAGCTCAATGTCTTCAATATCATTGCCAATTCAGGACCCGGAGCACATCTCACATTGAAGGAAATTGTATCTCAAATCTCAAATACAAATCCAAATGCAGCAGCTGCAAGTTTGAAGCGAATACTAAGAGTTCTCAGTGTTCATTCTCTGCTATCTGTATCTCAAAGGTCAAGCTCAAGTGGTAAGACAATGCTAGAAGAGACCTATGGTCTAACAAAGGAGACTCTTTGCCTAGTTCCAAATGAAGATGGAGTTTCCTTAGCCCCATATATTTTGTTAGCTTCAGAAATGCATACTGTGAAATGCTACTCCATGCTCAAAGACACAGTGCTTGAGCCTGGGAGCTTACTTTTCAAGAAGGCTCATGGTATGTCCGCATATGAGCTCATGTCCGATAAACCTGAGCTATCAAATTTATTTAATGAGGCTATTGGCCAAAGTACCGTCATCAATTTTGAAGATGTGTTAAAGGTTTACAAAGGTTTTGAAGAGGTAAAAGAGTTGATGGATGTTGGAGGAGGCAATGGAACTACAATTGCCAAAGTAGTATCTAGCTATCCACACATTCATGGGATTAACTTTGATTTGCCTAATGTTATCGCTCAAGCAACTGCTAAATATCAAGCTGGTGTGAAATATGTTGGTGGAGACATGTTTGAGTTGATACCAACTGCAGAGTCCATTATGTTGAAGTGGGTGCTTCATAATTGGGACGATGATCTATGCAAGAAAATATTAGAACGTTGTTGGGAGGCATTACCTGAAATCGGGAAGGTGATAATTGTCGAATTTGCATTACCCGAAATACCGGAGAAAACCACAGATTTAAAGAAAATAGTGGCATTAGACATCATGATGATGAGTATTGTTGGTGGTAGGCAACGAACAATTAATGAGTTTGATGGCCTATCGAAAGCTGCAGGCTTTGTTGAAACAAAGATTTTTCCGATTTCACATGGGTGTTATGTTATGGAGTTTCACAAAGTTAAAAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

363

Amino Acids

39.99

Weight (kDa)

6.17

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 15 - 104 3.2e-12 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 134 - 340 5.5e-57 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09630 FvH4_3g13140 FvH4_3g13141 FvH4_3g13160 FvH4_3g13170 FvH4_6g14540
malus_domestica MD10G1257600.v1.1
prunus_persica Prupe.4G086200_v2.0.a1
pyrus_communis pycom05g25920 pycom10g21380
rosa_chinensis RchiOBHm_Chr5g0014991 RchiOBHm_Chr5g0046111 RchiOBHm_Chr5g0056661 RchiOBHm_Chr7g0209941 RchiOBHm_Chr7g0211601 RchiOBHm_Chr7g0228691
rosa_laevigata RLG00000002994 RLG00000003075 RLG00000032124 RLG00000032125 RLG00000032158 RLG00000034388 RLG00000034564 RLG00000035118
rosa_multiflora Rmu_sc0003162.1_g000007 Rmu_sc0003162.1_g000010 Rmu_sc0003760.1_g000034 Rmu_sc0004232.1_g000016 Rmu_sc0004232.1_g000017 Rmu_sc0004232.1_g000023 Rmu_sc0004889.1_g000017 Rmu_sc0008669.1_g000001 Rmu_sc0009240.1_g000017 Rmu_sc0009255.1_g000012 Rmu_sc0013112.1_g000007
rosa_roxburghii Rroxscaffold_1G00023690 Rroxscaffold_1G00035370 Rroxscaffold_1G00035380 Rroxscaffold_1G00061590 Rroxscaffold_1G00061940 Rroxscaffold_3G00230880 Rroxscaffold_3G00247690
rosa_rugosa Rorug05G0019200 Rorug05G0022400 Rorug05G0225700 Rorug05G0246400 Rorug05G0301100 Rorug07G0117600 Rorug07G0126200
rosa_samantha Rh5AG114200 Rh5AG114300 Rh5AG117800 Rh5BG111300 Rh5BG115000 Rh5BG314800 Rh5BG383300 Rh5CG126300 Rh5CG359500 Rh5CG406400 Rh5DG110100 Rh5DG110600 Rh5DG114500 Rh5DG396000 Rh5DG396100 Rh7AG251400 Rh7AG403800 Rh7BG246000 Rh7CG268200 Rh7DG258000 Rh7DG266400
rosa_wichuraiana Rw5G009920 Rw5G010150 Rw5G028650 Rw5G034830 Rw7G021330 Rw7G022120 Rw7G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 468
AclI AACGTT 1 cut(s) 835
AcoI YGGCCR 1 cut(s) 526
AcsI RAATTY 3 cut(s) 46, 505, 881
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 1 cut(s) 535
AfiI CCNNNNNNNGG 1 cut(s) 860
AflIII ACRYGT 1 cut(s) 744
AgsI TTSAA 8 cut(s) 109, 151, 208, 448, 551, 581, 784, 1025
AhdI GACNNNNNGTC 1 cut(s) 300
AjnI CCWGG 1 cut(s) 430
AleI CACNNNNGTG 1 cut(s) 1051
Alw21I GWGCWC 3 cut(s) 99, 139, 480
Alw26I GTCTC 3 cut(s) 287, 308, 735
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 2 cut(s) 526, 997
ApeKI GCWGC 5 cut(s) 86, 191, 194, 197, 1010
ApoI RAATTY 3 cut(s) 46, 505, 881
AseI ATTAAT 1 cut(s) 984
AspS9I GGNCC 1 cut(s) 128
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 1 cut(s) 880
AvaII GGWCC 1 cut(s) 128
BalI TGGCCA 1 cut(s) 528
BanII GRGCYC 2 cut(s) 99, 480
BbsI GAAGAC 1 cut(s) 97
Bbv12I GWGCWC 3 cut(s) 99, 139, 480
BbvI GCAGC 5 cut(s) 73, 184, 203, 206, 997
BccI CCATC 3 cut(s) 335, 592, 989
BciT130I CCWGG 1 cut(s) 432
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 3 cut(s) 287, 308, 735
BfaI CTAG 4 cut(s) 59, 287, 326, 645
BfmI CTRYAG 2 cut(s) 765, 1011
BisI GCNGC 5 cut(s) 87, 192, 195, 198, 1011
BlsI GCNGC 5 cut(s) 88, 193, 196, 199, 1012
Bme1390I CCNGG 2 cut(s) 132, 432
Bme18I GGWCC 1 cut(s) 128
BmeRI GACNNNNNGTC 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 2 cut(s) 132, 432
BpiI GAAGAC 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 126, 158
Bpu10I CCTNAGC 2 cut(s) 352, 495
BpuEI CTTGAG 3 cut(s) 253, 446, 681
BpuMI CCSGG 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 431
BsaWI WCCGGW 1 cut(s) 901
Bsc4I CCNNNNNNNGG 1 cut(s) 860
Bse3DI GCAATG 2 cut(s) 114, 622
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 1 cut(s) 607
BseLI CCNNNNNNNGG 1 cut(s) 860
BseMI GCAATG 2 cut(s) 114, 622
BseMII CTCAG 2 cut(s) 241, 486
BseRI GAGGAG 1 cut(s) 624
BseXI GCAGC 5 cut(s) 73, 184, 203, 206, 997
BshFI GGCC 2 cut(s) 528, 999
BsiHKAI GWGCWC 3 cut(s) 99, 139, 480
BsiSI CCGG 2 cut(s) 132, 902
BslFI GGGAC 1 cut(s) 818
BslI CCNNNNNNNGG 1 cut(s) 860
BsmAI GTCTC 3 cut(s) 287, 308, 735
BsmFI GGGAC 1 cut(s) 818
BsnI GGCC 2 cut(s) 528, 999
Bso31I GGTCTC 1 cut(s) 287
Bsp1286I GDGCHC 3 cut(s) 99, 139, 480
Bsp143I GATC 1 cut(s) 811
BspACI CCGC 1 cut(s) 468
BspANI GGCC 2 cut(s) 528, 999
BspCNI CTCAG 2 cut(s) 240, 487
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 1 cut(s) 130
BspMAI CTGCAG 2 cut(s) 769, 1015
BspTNI GGTCTC 1 cut(s) 287
BsrDI GCAATG 2 cut(s) 114, 622
BssECI CCNNGG 1 cut(s) 431
BssMI GATC 1 cut(s) 811
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 3 cut(s) 391, 421, 538
Bst6I CTCTTC 2 cut(s) 285, 576
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 1 cut(s) 1015
BstDEI CTNAG 4 cut(s) 218, 227, 352, 495
BstF5I GGATG 1 cut(s) 607
BstKTI GATC 1 cut(s) 814
BstMAI GTCTC 3 cut(s) 287, 308, 735
BstMBI GATC 1 cut(s) 811
BstMWI GCNNNNNNNGC 2 cut(s) 83, 197
BstNI CCWGG 1 cut(s) 432
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 2 cut(s) 130, 430
BstSFI CTRYAG 2 cut(s) 765, 1011
BstV1I GCAGC 5 cut(s) 73, 184, 203, 206, 997
BstV2I GAAGAC 1 cut(s) 97
BsuRI GGCC 2 cut(s) 528, 999
BtsCI GGATG 1 cut(s) 607
BtsIMutI CAGTG 2 cut(s) 235, 426
Cac8I GCNNGC 1 cut(s) 1015
CaiI CAGNNNCTG 1 cut(s) 197
CciI TCATGA 1 cut(s) 945
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 534
CviAII CATG 7 cut(s) 406, 458, 481, 662, 745, 946, 1049
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 4 cut(s) 218, 227, 352, 495
DpnI GATC 1 cut(s) 813
DpnII GATC 1 cut(s) 811
DraI TTTAAA 2 cut(s) 81, 921
DriI GACNNNNNGTC 1 cut(s) 300
EaeI YGGCCR 1 cut(s) 526
Eam1104I CTCTTC 2 cut(s) 285, 576
Eam1105I GACNNNNNGTC 1 cut(s) 300
EarI CTCTTC 2 cut(s) 285, 576
Ecl136II GAGCTC 2 cut(s) 97, 478
Eco24I GRGCYC 2 cut(s) 99, 480
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 128
Eco53kI GAGCTC 2 cut(s) 97, 478
Eco57I CTGAAG 1 cut(s) 360
EcoICRI GAGCTC 2 cut(s) 97, 478
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 430
EcoT22I ATGCAT 1 cut(s) 387
EcoT38I GRGCYC 2 cut(s) 99, 480
FaeI CATG 7 cut(s) 409, 461, 484, 665, 748, 949, 1052
FaqI GGGAC 1 cut(s) 818
FatI CATG 7 cut(s) 405, 457, 480, 661, 744, 945, 1048
FauNDI CATATG 1 cut(s) 472
Fnu4HI GCNGC 5 cut(s) 87, 192, 195, 198, 1011
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 2 cut(s) 99, 480
Fsp4HI GCNGC 5 cut(s) 87, 192, 195, 198, 1011
FspBI CTAG 4 cut(s) 59, 287, 326, 645
GluI GCNGC 5 cut(s) 87, 192, 195, 198, 1011
HaeIII GGCC 2 cut(s) 528, 999
HapII CCGG 2 cut(s) 132, 902
Hin1II CATG 7 cut(s) 409, 461, 484, 665, 748, 949, 1052
HinfI GANTC 2 cut(s) 316, 770
HpaII CCGG 2 cut(s) 132, 902
HphI GGTGA 1 cut(s) 880
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 3 cut(s) 379, 487, 1041
Hpy188III TCNNGA 5 cut(s) 10, 126, 448, 862, 946
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 3 cut(s) 145, 445, 859
HpyCH4III ACNGT 3 cut(s) 391, 421, 538
HpyCH4IV ACGT 1 cut(s) 835
HpyCH4V TGCA 8 cut(s) 89, 191, 200, 385, 767, 819, 887, 1013
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 197
HpyF3I CTNAG 4 cut(s) 218, 227, 352, 495
HpySE526I ACGT 1 cut(s) 835
Hsp92II CATG 7 cut(s) 409, 461, 484, 665, 748, 949, 1052
Kzo9I GATC 1 cut(s) 811
LmnI GCTCC 2 cut(s) 134, 435
Lsp1109I GCAGC 5 cut(s) 73, 184, 203, 206, 997
MaeI CTAG 4 cut(s) 59, 287, 326, 645
MaeII ACGT 1 cut(s) 835
MaeIII GTNAC 1 cut(s) 64
MalI GATC 1 cut(s) 813
MboI GATC 1 cut(s) 811
MboII GAAGA 5 cut(s) 97, 302, 350, 563, 593
MfeI CAATTG 1 cut(s) 627
MhlI GDGCHC 3 cut(s) 99, 139, 480
MlsI TGGCCA 1 cut(s) 528
MluNI TGGCCA 1 cut(s) 528
MlyI GAGTC 2 cut(s) 310, 779
MmeI TCCRAC 1 cut(s) 586
MnlI CCTC 5 cut(s) 511, 577, 602, 605, 838
Mox20I TGGCCA 1 cut(s) 528
Mph1103I ATGCAT 1 cut(s) 387
MscI TGGCCA 1 cut(s) 528
MseI TTAA 7 cut(s) 80, 513, 560, 669, 920, 984, 1080
MslI CAYNNNNRTG 5 cut(s) 71, 660, 947, 950, 1051
Msp20I TGGCCA 1 cut(s) 528
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 2 cut(s) 132, 902
MspR9I CCNGG 2 cut(s) 132, 432
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 1 cut(s) 432
MwoI GCNNNNNNNGC 2 cut(s) 83, 197
NciI CCSGG 1 cut(s) 132
NdeI CATATG 1 cut(s) 472
NdeII GATC 1 cut(s) 811
NlaIII CATG 7 cut(s) 409, 461, 484, 665, 748, 949, 1052
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 64
NsiI ATGCAT 1 cut(s) 387
NspI RCATGY 1 cut(s) 748
OliI CACNNNNGTG 1 cut(s) 1051
PagI TCATGA 1 cut(s) 945
PciI ACATGT 1 cut(s) 744
PkrI GCNGC 5 cut(s) 88, 193, 196, 199, 1012
PleI GAGTC 2 cut(s) 310, 778
PpsI GAGTC 2 cut(s) 310, 778
PpuMI RGGWCCY 1 cut(s) 128
PscI ACATGT 1 cut(s) 744
PshBI ATTAAT 1 cut(s) 984
Psp124BI GAGCTC 2 cut(s) 99, 480
Psp1406I AACGTT 1 cut(s) 835
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 1 cut(s) 128
PspPPI RGGWCCY 1 cut(s) 128
PstI CTGCAG 2 cut(s) 769, 1015
PstNI CAGNNNCTG 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
RseI CAYNNNNRTG 5 cut(s) 71, 660, 947, 950, 1051
SacI GAGCTC 2 cut(s) 99, 480
SaqAI TTAA 7 cut(s) 80, 513, 560, 669, 920, 984, 1080
SatI GCNGC 5 cut(s) 87, 192, 195, 198, 1011
Sau3AI GATC 1 cut(s) 811
Sau96I GGNCC 1 cut(s) 128
SchI GAGTC 2 cut(s) 310, 779
ScrFI CCNGG 2 cut(s) 132, 432
SduI GDGCHC 3 cut(s) 99, 139, 480
SfcI CTRYAG 2 cut(s) 765, 1011
SinI GGWCC 1 cut(s) 128
SmiMI CAYNNNNRTG 5 cut(s) 71, 660, 947, 950, 1051
SmlI CTYRAG 3 cut(s) 268, 425, 696
SmoI CTYRAG 3 cut(s) 268, 425, 696
SsiI CCGC 1 cut(s) 468
SspI AATATT 1 cut(s) 828
SspMI CTAG 4 cut(s) 59, 287, 326, 645
SstI GAGCTC 2 cut(s) 99, 480
StyD4I CCNGG 2 cut(s) 130, 430
TaaI ACNGT 3 cut(s) 391, 421, 538
TaiI ACGT 1 cut(s) 838
TaqI TCGA 3 cut(s) 93, 879, 1004
Tru1I TTAA 7 cut(s) 80, 513, 560, 669, 920, 984, 1080
Tru9I TTAA 7 cut(s) 80, 513, 560, 669, 920, 984, 1080
TscAI CASTG 2 cut(s) 235, 426
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 5 cut(s) 86, 191, 194, 197, 1010
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 4 cut(s) 224, 351, 650, 785
TspRI CASTG 2 cut(s) 235, 426
VpaK11BI GGWCC 1 cut(s) 128
VspI ATTAAT 1 cut(s) 984
XapI RAATTY 3 cut(s) 46, 505, 881
XceI RCATGY 1 cut(s) 748
XspI CTAG 4 cut(s) 59, 287, 326, 645
Zsp2I ATGCAT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.