RLG00000016874

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001199
Physical Location & Seq
Forward (+)
10863636 .. 10864387
752 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016874

Sequence Viewer

Length: 657 bp
ATGGATGGTGGTGTATCTACGACTGGGAGGGCGGGGCTCTTGTCCCTCTTTTCTGGTTGGCGTGAGGGTAGCGGTGGCGGGTTTGTCGACGTTTTTTCCTATCTCTGGGCCTTTGATTGCTTCGCTCTTGGAAGCGATGGGGATCCGATGGTCTACCTTGATGACAGGTTTGAGATTTTGATGTCCAATTCGGATTCGGGTAAAAGACATAATGCTGTGAAGATGTCTGCTTCGGTGGTGTGTCGGTCTGCTACAGTACAACCAGATTTGCTTATTGGCTTTTTGGTTGGTAGAATGGTGGACAAGGGTGATGGCGATCCCGATACGGATGAGGGTCATCTCTCGGTGGATGTTGGTGGTGCTTGGTGGCTGAGGGTTCTCTCAGTTCACTTCCCGGATCACTGTTTGCCTAGTTTTAAAGGCTTGTGTAAGAGTTTCTGCGATGGCGGCGGTCAAGGTGGAGGCGGCAGCCAAGAGGTAACGTTAATGGAGTTTGCTAGGGTTTTTGCAACCTTAGATTGTTGGCCTCCACATTGGTTATTGGGCTGGAACTTGACTGAGCTGCTCTGGGTCCAAAAGGCTCTTAGGGTTCTTTTTTTTATTTTATTTTATGTTTTAAGTTTAGCATCCTTCCTTATTCTTTTAAGGAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

23.88

Weight (kDa)

5.1

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 87, 153
AciI CCGC 6 cut(s) 32, 72, 78, 447, 450, 465
AclI AACGTT 1 cut(s) 482
AclWI GGATC 4 cut(s) 137, 150, 311, 405
AfaI GTAC 1 cut(s) 258
AfiI CCNNNNNNNGG 1 cut(s) 105
AluBI AGCT 1 cut(s) 562
AluI AGCT 1 cut(s) 562
AlwI GGATC 4 cut(s) 137, 150, 311, 405
AoxI GGCC 2 cut(s) 108, 524
ApeKI GCWGC 2 cut(s) 468, 562
AspS9I GGNCC 2 cut(s) 108, 571
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 1 cut(s) 571
BamHI GGATCC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 39
BbvCI CCTCAGC 1 cut(s) 371
BbvI GCAGC 2 cut(s) 480, 549
BccI CCATC 4 cut(s) 131, 142, 305, 437
BcnI CCSGG 1 cut(s) 395
BfaI CTAG 2 cut(s) 411, 498
BfmI CTRYAG 1 cut(s) 252
BisI GCNGC 4 cut(s) 448, 466, 469, 563
BlsI GCNGC 4 cut(s) 449, 467, 470, 564
Bme1390I CCNGG 1 cut(s) 395
Bme18I GGWCC 1 cut(s) 571
BmgT120I GGNCC 2 cut(s) 108, 571
BmiI GGNNCC 2 cut(s) 144, 572
BmrFI CCNGG 1 cut(s) 395
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 1 cut(s) 635
BmuI ACTGGG 1 cut(s) 33
Bpu10I CCTNAGC 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 395
BsaBI GATNNNNATC 2 cut(s) 141, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 1 cut(s) 28
Bse8I GATNNNNATC 2 cut(s) 141, 315
BseGI GGATG 4 cut(s) 10, 334, 355, 626
BseJI GATNNNNATC 2 cut(s) 141, 315
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMII CTCAG 3 cut(s) 362, 396, 549
BseNI ACTGG 1 cut(s) 28
BseXI GCAGC 2 cut(s) 480, 549
BshFI GGCC 2 cut(s) 110, 526
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 28
BsnI GGCC 2 cut(s) 110, 526
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 142, 316, 397
BspACI CCGC 6 cut(s) 32, 72, 78, 447, 450, 465
BspANI GGCC 2 cut(s) 110, 526
BspCNI CTCAG 3 cut(s) 363, 395, 550
BspLI GGNNCC 2 cut(s) 144, 572
BspPI GGATC 4 cut(s) 137, 150, 311, 405
BsrI ACTGG 1 cut(s) 28
BssMI GATC 3 cut(s) 142, 316, 397
Bst4CI ACNGT 2 cut(s) 256, 404
BstDEI CTNAG 5 cut(s) 371, 382, 514, 558, 584
BstF5I GGATG 4 cut(s) 10, 334, 355, 626
BstKTI GATC 3 cut(s) 145, 319, 400
BstMBI GATC 3 cut(s) 142, 316, 397
BstMWI GCNNNNNNNGC 1 cut(s) 447
BstSCI CCNGG 1 cut(s) 393
BstSFI CTRYAG 1 cut(s) 252
BstV1I GCAGC 2 cut(s) 480, 549
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BsuRI GGCC 2 cut(s) 110, 526
BtgZI GCGATG 2 cut(s) 150, 456
BtsCI GGATG 4 cut(s) 10, 334, 355, 626
BtsIMutI CAGTG 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 108, 571
Csp6I GTAC 1 cut(s) 257
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 5 cut(s) 371, 382, 514, 558, 584
DpnI GATC 3 cut(s) 144, 318, 399
DpnII GATC 3 cut(s) 142, 316, 397
DraI TTTAAA 1 cut(s) 418
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 571
EcoT38I GRGCYC 1 cut(s) 39
FaiI YATR 2 cut(s) 210, 612
FaqI GGGAC 1 cut(s) 28
FauI CCCGC 2 cut(s) 25, 71
FblI GTMKAC 2 cut(s) 87, 153
Fnu4HI GCNGC 4 cut(s) 448, 466, 469, 563
FokI GGATG 4 cut(s) 17, 341, 362, 613
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 4 cut(s) 448, 466, 469, 563
FspBI CTAG 2 cut(s) 411, 498
GluI GCNGC 4 cut(s) 448, 466, 469, 563
HaeIII GGCC 2 cut(s) 110, 526
HapII CCGG 1 cut(s) 395
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HinfI GANTC 1 cut(s) 194
HpaII CCGG 1 cut(s) 395
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 4 cut(s) 88, 154, 301, 388
Hpy188I TCNGA 2 cut(s) 147, 193
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 4 cut(s) 88, 154, 301, 388
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 640
HpyCH4III ACNGT 2 cut(s) 256, 404
HpyCH4IV ACGT 2 cut(s) 90, 482
HpyCH4V TGCA 1 cut(s) 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 447
HpyF3I CTNAG 5 cut(s) 371, 382, 514, 558, 584
HpySE526I ACGT 2 cut(s) 90, 482
Kzo9I GATC 3 cut(s) 142, 316, 397
LpnPI CCDG 8 cut(s) 9, 39, 91, 151, 276, 408, 532, 553
Lsp1109I GCAGC 2 cut(s) 480, 549
LweI GCATC 1 cut(s) 635
MaeI CTAG 2 cut(s) 411, 498
MaeII ACGT 2 cut(s) 90, 482
MaeIII GTNAC 1 cut(s) 478
MalI GATC 3 cut(s) 144, 318, 399
MboI GATC 3 cut(s) 142, 316, 397
MboII GAAGA 1 cut(s) 232
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 1 cut(s) 187
MnlI CCTC 8 cut(s) 21, 56, 58, 325, 366, 455, 469, 537
MseI TTAA 4 cut(s) 417, 485, 617, 644
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 395
MwoI GCNNNNNNNGC 1 cut(s) 447
NciI CCSGG 1 cut(s) 395
NdeII GATC 3 cut(s) 142, 316, 397
NlaIV GGNNCC 2 cut(s) 144, 572
PfeI GAWTC 1 cut(s) 194
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 4 cut(s) 449, 467, 470, 564
Psp1406I AACGTT 1 cut(s) 482
PspN4I GGNNCC 2 cut(s) 144, 572
PspPI GGNCC 2 cut(s) 108, 571
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
SalI GTCGAC 1 cut(s) 86
SaqAI TTAA 4 cut(s) 417, 485, 617, 644
SatI GCNGC 4 cut(s) 448, 466, 469, 563
Sau3AI GATC 3 cut(s) 142, 316, 397
Sau96I GGNCC 2 cut(s) 108, 571
ScrFI CCNGG 1 cut(s) 395
SduI GDGCHC 1 cut(s) 39
SetI ASST 8 cut(s) 93, 159, 170, 460, 480, 485, 515, 564
SfaNI GCATC 1 cut(s) 635
SfcI CTRYAG 1 cut(s) 252
SinI GGWCC 1 cut(s) 571
Sse9I AATT 1 cut(s) 187
SsiI CCGC 6 cut(s) 32, 72, 78, 447, 450, 465
SspMI CTAG 2 cut(s) 411, 498
StyD4I CCNGG 1 cut(s) 393
TaaI ACNGT 2 cut(s) 256, 404
TaiI ACGT 2 cut(s) 93, 485
TaqI TCGA 1 cut(s) 87
TaqII GACCGA 1 cut(s) 234
TasI AATT 1 cut(s) 187
TatI WGTACW 1 cut(s) 256
TauI GCSGC 2 cut(s) 450, 468
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 4 cut(s) 417, 485, 617, 644
Tru9I TTAA 4 cut(s) 417, 485, 617, 644
TscAI CASTG 1 cut(s) 407
TseI GCWGC 2 cut(s) 468, 562
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 571
XmiI GTMKAC 2 cut(s) 87, 153
XspI CTAG 2 cut(s) 411, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.