Rh6AG032000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001199
Physical Location & Seq
Forward (+)
3928183 .. 3928434
252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG032000.1

Sequence Viewer

Length: 252 bp
ATGGTGGCGTTGGAGGACCATACCCAGCTCAGAACCGGGTCGTTGTTGGCGATGGGCAACAGGGAGAGGTTGGCGCCGTCGGACTCGAAGATGGAGAGGAGCCACTTCATGTCGCCGATGGAGGACTCGAGGAGGTTGGAGACCTTGCGGAAGTCGGCGGTGGTGGTGATGGAGAAGACCTGGCGGAGGACGTGGAGATACCAAAGGATCAGCTTCGCGGTCCGAAGAAATGGGTTGATGAGGGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

83

Amino Acids

9.91

Weight (kDa)

11.03

Isoelectric Point (pI)

63.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AccII CGCG 1 cut(s) 218
AciI CCGC 4 cut(s) 148, 158, 184, 218
AclWI GGATC 1 cut(s) 215
AcyI GRCGYC 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 186
AjiI CACGTC 1 cut(s) 192
AjnI CCWGG 1 cut(s) 179
AluBI AGCT 2 cut(s) 28, 213
AluI AGCT 2 cut(s) 28, 213
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 127
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 2 cut(s) 16, 220
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 2 cut(s) 16, 220
BanI GGYRCC 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 182
BccI CCATC 4 cut(s) 46, 85, 112, 163
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 1 cut(s) 181
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 1 cut(s) 134
BfoI RGCGCY 1 cut(s) 77
Bme1390I CCNGG 2 cut(s) 37, 181
Bme18I GGWCC 2 cut(s) 16, 220
BmeT110I CYCGRG 1 cut(s) 127
BmgBI CACGTC 1 cut(s) 192
BmgT120I GGNCC 2 cut(s) 16, 220
BmiI GGNNCC 2 cut(s) 75, 101
BmrFI CCNGG 2 cut(s) 37, 181
BpiI GAAGAC 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 37
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 134
Bsc4I CCNNNNNNNGG 1 cut(s) 186
BseBI CCWGG 1 cut(s) 181
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMII CTCAG 1 cut(s) 43
BseRI GAGGAG 2 cut(s) 112, 145
BseYI CCCAGC 1 cut(s) 24
Bsh1236I CGCG 1 cut(s) 218
BshNI GGYRCC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 134
Bso31I GGTCTC 1 cut(s) 134
BsoBI CYCGRG 1 cut(s) 127
Bsp143I GATC 1 cut(s) 207
BspACI CCGC 4 cut(s) 148, 158, 184, 218
BspCNI CTCAG 1 cut(s) 42
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 2 cut(s) 75, 101
BspPI GGATC 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 134
BssMI GATC 1 cut(s) 207
BssNI GRCGYC 1 cut(s) 74
Bst2UI CCWGG 1 cut(s) 181
BstACI GRCGYC 1 cut(s) 74
BstDEI CTNAG 1 cut(s) 29
BstENI CCTNNNNNAGG 1 cut(s) 184
BstFNI CGCG 1 cut(s) 218
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 1 cut(s) 210
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 1 cut(s) 207
BstNI CCWGG 1 cut(s) 181
BstSCI CCNGG 2 cut(s) 35, 179
BstUI CGCG 1 cut(s) 218
BstV2I GAAGAC 1 cut(s) 182
BtgZI GCGATG 1 cut(s) 65
BtrI CACGTC 1 cut(s) 192
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 16, 220
CpoI CGGWCCG 1 cut(s) 220
CspI CGGWCCG 1 cut(s) 220
CviAII CATG 1 cut(s) 109
CviJI RGCY 3 cut(s) 28, 102, 213
CviKI_1 RGCY 3 cut(s) 28, 102, 213
DdeI CTNAG 1 cut(s) 29
DinI GGCGCC 1 cut(s) 75
DpnI GATC 1 cut(s) 209
DpnII GATC 1 cut(s) 207
EciI GGCGGA 1 cut(s) 199
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 2 cut(s) 16, 220
Eco88I CYCGRG 1 cut(s) 127
EcoNI CCTNNNNNAGG 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 179
EgeI GGCGCC 1 cut(s) 75
EheI GGCGCC 1 cut(s) 75
FaeI CATG 1 cut(s) 112
FaiI YATR 2 cut(s) 21, 110
FatI CATG 1 cut(s) 108
GlaI GCGC 1 cut(s) 75
GsaI CCCAGC 1 cut(s) 28
HaeII RGCGCY 1 cut(s) 77
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 1 cut(s) 112
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 2 cut(s) 83, 125
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 178
Hpy188I TCNGA 3 cut(s) 32, 82, 224
Hpy99I CGWCG 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 191
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 1 cut(s) 191
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 1 cut(s) 112
HspAI GCGC 1 cut(s) 74
KasI GGCGCC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 207
LmnI GCTCC 1 cut(s) 99
LpnPI CCDG 5 cut(s) 38, 46, 49, 166, 193
MaeII ACGT 1 cut(s) 191
MalI GATC 1 cut(s) 209
MboI GATC 1 cut(s) 207
MboII GAAGA 3 cut(s) 100, 187, 237
Mly113I GGCGCC 1 cut(s) 74
MlyI GAGTC 2 cut(s) 77, 119
MmeI TCCRAC 2 cut(s) 60, 117
MnlI CCTC 9 cut(s) 7, 60, 90, 115, 123, 126, 180, 234, 238
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 37, 181
MvaI CCWGG 1 cut(s) 181
MvnI CGCG 1 cut(s) 218
NarI GGCGCC 1 cut(s) 74
NciI CCSGG 1 cut(s) 37
NdeII GATC 1 cut(s) 207
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 2 cut(s) 75, 101
PaeR7I CTCGAG 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PleI GAGTC 2 cut(s) 77, 119
PluTI GGCGCC 1 cut(s) 77
PpsI GAGTC 2 cut(s) 77, 119
Psp6I CCWGG 1 cut(s) 179
PspFI CCCAGC 1 cut(s) 24
PspGI CCWGG 1 cut(s) 179
PspN4I GGNNCC 2 cut(s) 75, 101
PspPI GGNCC 2 cut(s) 16, 220
PspXI VCTCGAGB 1 cut(s) 127
Rsr2I CGGWCCG 1 cut(s) 220
RsrII CGGWCCG 1 cut(s) 220
Sau3AI GATC 1 cut(s) 207
Sau96I GGNCC 2 cut(s) 16, 220
SchI GAGTC 2 cut(s) 77, 119
ScrFI CCNGG 2 cut(s) 37, 181
SetI ASST 7 cut(s) 30, 71, 137, 146, 182, 194, 215
SfoI GGCGCC 1 cut(s) 75
Sfr274I CTCGAG 1 cut(s) 127
SinI GGWCC 2 cut(s) 16, 220
SlaI CTCGAG 1 cut(s) 127
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
SsiI CCGC 4 cut(s) 148, 158, 184, 218
SspDI GGCGCC 1 cut(s) 73
StyD4I CCNGG 2 cut(s) 35, 179
TaiI ACGT 1 cut(s) 194
TaqI TCGA 2 cut(s) 86, 128
TspDTI ATGAA 1 cut(s) 97
VpaK11BI GGWCC 2 cut(s) 16, 220
XagI CCTNNNNNAGG 1 cut(s) 184
XhoI CTCGAG 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.