Rroxscaffold_4G00322100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001199
Physical Location & Seq
Forward (+)
52036842 .. 52037833
992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00322100.1

Sequence Viewer

Length: 498 bp
ATGTGGTGGAGTCAAATGCTTCAGGAGTATTTCTCCTTGGAGATTCAAGATAAGTATGCAATAATCTTTGACCTACTGTCAAACAAGACTGCTAATGAAACAGCGACGTCCCTTACTTCCAAAGAGAGATTTTCCTTTACAGTCCACTCATTCGAGCCCTCTAATCTTTTACTCATTCCTGTTGGTAGCCCGAAACGCTTCAGGTTGGGACTAGCGCAAAAAGCCATCCTAGTCCCCGCAGGGCATAAACGTCTCGAGCCGGAAGTTGAACTAAGTCAGGTTCATAGGGCCGGGAAAATAAGCAGAACGGCACAGAACTCCACCCCTCCTATCGAGACTCCAGTGACTAGTGAACTTACATCAGCGGGTGATCTACCCACTTCACCTTCAACACAGATTGTGACACTTCCTGAGCCCCTTTCTTCAAGCTCTAGTGCTGAAGACGCTTCCTGTTCCAAAGCTGGAGAAGTTAAGGAACCACTAGAACTTGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.09

Weight (kDa)

5.14

Isoelectric Point (pI)

58.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 110
AciI CCGC 2 cut(s) 237, 365
AcuI CTGAAG 3 cut(s) 5, 184, 459
AcyI GRCGYC 1 cut(s) 107
AgsI TTSAA 4 cut(s) 47, 269, 390, 426
AhdI GACNNNNNGTC 1 cut(s) 76
AhlI ACTAGT 1 cut(s) 347
AluBI AGCT 2 cut(s) 429, 461
AluI AGCT 2 cut(s) 429, 461
Alw26I GTCTC 2 cut(s) 257, 329
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 1 cut(s) 288
Asp700I GAANNNNTTC 1 cut(s) 197
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 1 cut(s) 288
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 2 cut(s) 375, 380
AvaI CYCGRG 1 cut(s) 254
BanII GRGCYC 2 cut(s) 159, 417
BbsI GAAGAC 1 cut(s) 447
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 324
BcnI CCSGG 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 257, 329
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 5 cut(s) 212, 230, 348, 432, 482
Bme1390I CCNGG 1 cut(s) 292
BmeRI GACNNNNNGTC 1 cut(s) 76
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 1 cut(s) 292
BpiI GAAGAC 1 cut(s) 447
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpmI CTGGAG 2 cut(s) 324, 483
Bpu10I CCTNAGC 1 cut(s) 411
BpuMI CCSGG 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 341
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 341
BshFI GGCC 1 cut(s) 290
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 2 cut(s) 260, 291
BslFI GGGAC 3 cut(s) 94, 218, 222
BsmAI GTCTC 2 cut(s) 257, 329
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 3 cut(s) 94, 218, 222
BsnI GGCC 1 cut(s) 290
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 2 cut(s) 159, 417
Bsp143I GATC 1 cut(s) 370
BspACI CCGC 2 cut(s) 237, 365
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 477
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 1 cut(s) 370
BssNI GRCGYC 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 78, 142
BstACI GRCGYC 1 cut(s) 107
BstDEI CTNAG 2 cut(s) 272, 411
BstF5I GGATG 1 cut(s) 225
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 1 cut(s) 373
BstMAI GTCTC 2 cut(s) 257, 329
BstMBI GATC 1 cut(s) 370
BstMWI GCNNNNNNNGC 3 cut(s) 195, 221, 443
BstSCI CCNGG 1 cut(s) 290
BstV2I GAAGAC 1 cut(s) 447
BsuRI GGCC 1 cut(s) 290
BtsCI GGATG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 348
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 1 cut(s) 288
CseI GACGC 1 cut(s) 452
CviJI RGCY 8 cut(s) 157, 189, 224, 259, 290, 415, 429, 461
CviKI_1 RGCY 8 cut(s) 157, 189, 224, 259, 290, 415, 429, 461
DdeI CTNAG 2 cut(s) 272, 411
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
DriI GACNNNNNGTC 1 cut(s) 76
Eam1105I GACNNNNNGTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 2 cut(s) 159, 417
Eco57I CTGAAG 3 cut(s) 5, 184, 459
Eco88I CYCGRG 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 36
EcoT38I GRGCYC 2 cut(s) 159, 417
ErhI CCWWGG 1 cut(s) 36
Esp3I CGTCTC 1 cut(s) 257
FaiI YATR 3 cut(s) 57, 246, 285
FaqI GGGAC 3 cut(s) 94, 218, 222
FauI CCCGC 2 cut(s) 244, 358
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 2 cut(s) 159, 417
FspBI CTAG 5 cut(s) 212, 230, 348, 432, 482
GlaI GCGC 1 cut(s) 216
GsuI CTGGAG 2 cut(s) 324, 483
HaeIII GGCC 1 cut(s) 290
HapII CCGG 2 cut(s) 260, 291
HgaI GACGC 1 cut(s) 452
HhaI GCGC 1 cut(s) 217
Hin1I GRCGYC 1 cut(s) 107
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HinfI GANTC 3 cut(s) 10, 43, 337
HpaII CCGG 2 cut(s) 260, 291
HphI GGTGA 2 cut(s) 375, 380
Hpy166II GTNNAC 2 cut(s) 145, 353
Hpy188III TCNNGA 5 cut(s) 23, 47, 254, 334, 410
Hpy8I GTNNAC 2 cut(s) 145, 353
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 2 cut(s) 78, 142
HpyCH4IV ACGT 2 cut(s) 107, 250
HpyCH4V TGCA 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 3 cut(s) 195, 221, 443
HpyF3I CTNAG 2 cut(s) 272, 411
HpySE526I ACGT 2 cut(s) 107, 250
Hsp92I GRCGYC 1 cut(s) 107
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 1 cut(s) 370
MaeI CTAG 5 cut(s) 212, 230, 348, 432, 482
MaeII ACGT 2 cut(s) 107, 250
MaeIII GTNAC 2 cut(s) 343, 400
MalI GATC 1 cut(s) 372
MboI GATC 1 cut(s) 370
MboII GAAGA 2 cut(s) 414, 452
MhlI GDGCHC 2 cut(s) 159, 417
MlyI GAGTC 2 cut(s) 19, 331
MnlI CCTC 2 cut(s) 169, 336
MroXI GAANNNNTTC 1 cut(s) 197
MseI TTAA 1 cut(s) 471
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 2 cut(s) 260, 291
MspR9I CCNGG 1 cut(s) 292
MwoI GCNNNNNNNGC 3 cut(s) 195, 221, 443
NciI CCSGG 1 cut(s) 292
NdeII GATC 1 cut(s) 370
NlaIV GGNNCC 1 cut(s) 477
NmuCI GTSAC 2 cut(s) 343, 400
PaeR7I CTCGAG 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 43
PleI GAGTC 2 cut(s) 18, 331
PpsI GAGTC 2 cut(s) 18, 331
PspN4I GGNNCC 1 cut(s) 477
PspPI GGNCC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 471
Sau3AI GATC 1 cut(s) 370
Sau96I GGNCC 1 cut(s) 288
SchI GAGTC 2 cut(s) 19, 331
ScrFI CCNGG 1 cut(s) 292
SduI GDGCHC 2 cut(s) 159, 417
SetI ASST 8 cut(s) 75, 110, 206, 253, 282, 388, 431, 463
Sfr274I CTCGAG 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SpeI ACTAGT 1 cut(s) 347
SsiI CCGC 2 cut(s) 237, 365
SspMI CTAG 5 cut(s) 212, 230, 348, 432, 482
StyD4I CCNGG 1 cut(s) 290
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 78, 142
TaiI ACGT 2 cut(s) 110, 253
TaqI TCGA 3 cut(s) 153, 255, 333
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 2 cut(s) 343, 400
Tsp45I GTSAC 2 cut(s) 343, 400
TspDTI ATGAA 2 cut(s) 111, 272
TspRI CASTG 1 cut(s) 348
XhoI CTCGAG 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 5 cut(s) 212, 230, 348, 432, 482
ZraI GACGTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.