Rroxscaffold_4G00318570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001199
Physical Location & Seq
Reverse (-)
44766201 .. 44767524
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00318570.1

Sequence Viewer

Length: 633 bp
ATGTTGGACTACTTCATTCTTCGAAGCTATGGGGATACGATGGTCTGCCTTGATGATAGGTCCGGGATTTTGATGTCCAATTCGGATTCGGGTAAAAGACCTAATGTGGCGAAGATGTCCGCTTTGTGGTTTGCTGCAGTTCAACCAGATTTGCTTATTGGCTTTTTGGTTGGTAGAATGGTGGACAAGGGTAATGGCTATCCCGATATCTATACGGACGATGGTCGTCTCTCAATGGACGATGGTGGTGCTTGGTGGCCAAGTGGTCCGTCGGTTCACTTACCGGAACACTATTTGTCCGGTTTTGGAGGCTTGTGTAAGAATTTCTGCAATGGCGGAGGTTCTCCGGTTGGAAGGTACTGCGGTGGTGGTCAAAGTGGTGGCGATAGCCGGGAGGTAACCTTGATAGAGTCTGCTAGGGTGGTTTATGCAACCTTGGATTGTTGGGCTCCATATTGGTTATTGGGCCGGAACTCGGACGGGCTGCTTTGGGTCCAAAAGGCCCTTAGGCCCCTCGGTGAGACAATTTTGAGGCAAACAATGGCACTTGGGTCCTACCGAATATCCAACAAGGATCTGATTATCTTTACGGTGGCTGTTTCTCGATCCCTTGTTGGATCTACCTCATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

22.76

Weight (kDa)

6.09

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 120, 336, 363
AclWI GGATC 3 cut(s) 582, 600, 625
AcoI YGGCCR 1 cut(s) 257
AcsI RAATTY 1 cut(s) 322
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 126, 475
AgsI TTSAA 1 cut(s) 143
AluBI AGCT 1 cut(s) 27
AluI AGCT 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 233, 515
AlwI GGATC 3 cut(s) 582, 600, 625
AoxI GGCC 4 cut(s) 257, 466, 501, 509
ApeKI GCWGC 2 cut(s) 134, 484
ApoI RAATTY 1 cut(s) 322
AspS9I GGNCC 7 cut(s) 60, 266, 466, 493, 502, 510, 552
AsuC2I CCSGG 2 cut(s) 64, 392
AsuHPI GGTGA 1 cut(s) 530
AsuII TTCGAA 1 cut(s) 22
AvaII GGWCC 4 cut(s) 60, 266, 493, 552
AxyI CCTNAGG 1 cut(s) 506
BaeI ACNNNNGTAYC 4 cut(s) 27, 60, 349, 382
BalI TGGCCA 1 cut(s) 259
BanII GRGCYC 1 cut(s) 451
BbvI GCAGC 2 cut(s) 121, 471
BccI CCATC 3 cut(s) 34, 215, 236
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciVI GTATCC 1 cut(s) 28
BcnI CCSGG 2 cut(s) 64, 392
BcoDI GTCTC 2 cut(s) 233, 515
BfaI CTAG 1 cut(s) 417
BfmI CTRYAG 1 cut(s) 135
BfuI GTATCC 1 cut(s) 28
BisI GCNGC 2 cut(s) 135, 485
BlsI GCNGC 2 cut(s) 136, 486
Bme1390I CCNGG 2 cut(s) 64, 392
Bme18I GGWCC 4 cut(s) 60, 266, 493, 552
BmgT120I GGNCC 7 cut(s) 60, 266, 466, 493, 502, 510, 552
BmiI GGNNCC 4 cut(s) 450, 494, 512, 553
BmrFI CCNGG 2 cut(s) 64, 392
BoxI GACNNNNGTC 1 cut(s) 222
Bpu14I TTCGAA 1 cut(s) 22
BpuMI CCSGG 2 cut(s) 64, 392
BsaJI CCNNGG 2 cut(s) 435, 514
BsaWI WCCGGW 3 cut(s) 283, 299, 346
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 475
Bse21I CCTNAGG 1 cut(s) 506
Bse3DI GCAATG 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 435, 514
BseLI CCNNNNNNNGG 2 cut(s) 126, 475
BseMI GCAATG 1 cut(s) 337
BseXI GCAGC 2 cut(s) 121, 471
BshFI GGCC 4 cut(s) 259, 468, 503, 511
BsiSI CCGG 6 cut(s) 63, 284, 300, 347, 391, 469
BslI CCNNNNNNNGG 2 cut(s) 126, 475
BsmAI GTCTC 2 cut(s) 233, 515
BsmBI CGTCTC 1 cut(s) 233
BsnI GGCC 4 cut(s) 259, 468, 503, 511
Bsp119I TTCGAA 1 cut(s) 22
Bsp1286I GDGCHC 1 cut(s) 451
Bsp143I GATC 3 cut(s) 574, 605, 617
BspACI CCGC 3 cut(s) 120, 336, 363
BspANI GGCC 4 cut(s) 259, 468, 503, 511
BspLI GGNNCC 4 cut(s) 450, 494, 512, 553
BspMAI CTGCAG 1 cut(s) 139
BspPI GGATC 3 cut(s) 582, 600, 625
BspT104I TTCGAA 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 337
BssECI CCNNGG 2 cut(s) 435, 514
BssMI GATC 3 cut(s) 574, 605, 617
BssT1I CCWWGG 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 592
BstBI TTCGAA 1 cut(s) 22
BstDEI CTNAG 1 cut(s) 506
BstEII GGTNACC 1 cut(s) 397
BstKTI GATC 3 cut(s) 577, 608, 620
BstMAI GTCTC 2 cut(s) 233, 515
BstMBI GATC 3 cut(s) 574, 605, 617
BstPAI GACNNNNGTC 1 cut(s) 222
BstPI GGTNACC 1 cut(s) 397
BstSCI CCNGG 2 cut(s) 62, 390
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 121, 471
BstX2I RGATCY 2 cut(s) 574, 617
BstYI RGATCY 2 cut(s) 574, 617
Bsu36I CCTNAGG 1 cut(s) 506
BsuI GTATCC 1 cut(s) 28
BsuRI GGCC 4 cut(s) 259, 468, 503, 511
Cfr13I GGNCC 7 cut(s) 60, 266, 466, 493, 502, 510, 552
Csp6I GTAC 1 cut(s) 358
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 1 cut(s) 506
DpnI GATC 3 cut(s) 576, 607, 619
DpnII GATC 3 cut(s) 574, 605, 617
EaeI YGGCCR 1 cut(s) 257
EciI GGCGGA 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 451
Eco32I GATATC 1 cut(s) 208
Eco47I GGWCC 4 cut(s) 60, 266, 493, 552
Eco81I CCTNAGG 1 cut(s) 506
Eco91I GGTNACC 1 cut(s) 397
EcoO109I RGGNCCY 3 cut(s) 502, 510, 552
EcoO65I GGTNACC 1 cut(s) 397
EcoRV GATATC 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 233
FaiI YATR 4 cut(s) 30, 213, 429, 454
Fnu4HI GCNGC 2 cut(s) 135, 485
FriOI GRGCYC 1 cut(s) 451
Fsp4HI GCNGC 2 cut(s) 135, 485
FspBI CTAG 1 cut(s) 417
GluI GCNGC 2 cut(s) 135, 485
HaeIII GGCC 4 cut(s) 259, 468, 503, 511
HapII CCGG 6 cut(s) 63, 284, 300, 347, 391, 469
HinfI GANTC 2 cut(s) 86, 410
HpaII CCGG 6 cut(s) 63, 284, 300, 347, 391, 469
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 2 cut(s) 184, 277
Hpy188I TCNGA 3 cut(s) 85, 478, 579
Hpy188III TCNNGA 2 cut(s) 203, 603
Hpy8I GTNNAC 2 cut(s) 184, 277
Hpy99I CGWCG 1 cut(s) 274
HpyAV CCTTC 1 cut(s) 348
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4V TGCA 3 cut(s) 137, 330, 431
HpyF3I CTNAG 1 cut(s) 506
Kzo9I GATC 3 cut(s) 574, 605, 617
LmnI GCTCC 1 cut(s) 454
LpnPI CCDG 7 cut(s) 76, 159, 297, 313, 360, 404, 482
Lsp1109I GCAGC 2 cut(s) 121, 471
MaeI CTAG 1 cut(s) 417
MaeIII GTNAC 1 cut(s) 397
MalI GATC 3 cut(s) 576, 607, 619
MboI GATC 3 cut(s) 574, 605, 617
MboII GAAGA 2 cut(s) 11, 124
MflI RGATCY 2 cut(s) 574, 617
MhlI GDGCHC 1 cut(s) 451
MlsI TGGCCA 1 cut(s) 259
MluCI AATT 3 cut(s) 79, 322, 525
MluNI TGGCCA 1 cut(s) 259
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 3 cut(s) 331, 591, 595
MnlI CCTC 5 cut(s) 302, 332, 388, 524, 525
Mox20I TGGCCA 1 cut(s) 259
MscI TGGCCA 1 cut(s) 259
Msp20I TGGCCA 1 cut(s) 259
MspI CCGG 6 cut(s) 63, 284, 300, 347, 391, 469
MspR9I CCNGG 2 cut(s) 64, 392
NciI CCSGG 2 cut(s) 64, 392
NdeII GATC 3 cut(s) 574, 605, 617
NlaIV GGNNCC 4 cut(s) 450, 494, 512, 553
NspV TTCGAA 1 cut(s) 22
PfeI GAWTC 1 cut(s) 86
PfoI TCCNGGA 1 cut(s) 62
PkrI GCNGC 2 cut(s) 136, 486
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PpuMI RGGWCCY 1 cut(s) 552
PshAI GACNNNNGTC 1 cut(s) 222
Psp5II RGGWCCY 1 cut(s) 552
PspEI GGTNACC 1 cut(s) 397
PspN4I GGNNCC 4 cut(s) 450, 494, 512, 553
PspPI GGNCC 7 cut(s) 60, 266, 466, 493, 502, 510, 552
PspPPI RGGWCCY 1 cut(s) 552
PstI CTGCAG 1 cut(s) 139
PsuI RGATCY 2 cut(s) 574, 617
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SatI GCNGC 2 cut(s) 135, 485
Sau3AI GATC 3 cut(s) 574, 605, 617
Sau96I GGNCC 7 cut(s) 60, 266, 466, 493, 502, 510, 552
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 2 cut(s) 64, 392
SduI GDGCHC 1 cut(s) 451
SetI ASST 9 cut(s) 29, 62, 103, 343, 359, 399, 404, 437, 626
SfcI CTRYAG 1 cut(s) 135
SfuI TTCGAA 1 cut(s) 22
SinI GGWCC 4 cut(s) 60, 266, 493, 552
Sse9I AATT 3 cut(s) 79, 322, 525
SsiI CCGC 3 cut(s) 120, 336, 363
SspMI CTAG 1 cut(s) 417
StyD4I CCNGG 2 cut(s) 62, 390
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 592
TaqI TCGA 2 cut(s) 22, 604
TasI AATT 3 cut(s) 79, 322, 525
TfiI GAWTC 1 cut(s) 86
TseI GCWGC 2 cut(s) 134, 484
TspDTI ATGAA 1 cut(s) 4
TspGWI ACGGA 2 cut(s) 230, 258
VpaK11BI GGWCC 4 cut(s) 60, 266, 493, 552
XapI RAATTY 1 cut(s) 322
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.