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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 131 |
| AciI | CCGC | 2 cut(s) | 119, 151 |
| AclWI | GGATC | 1 cut(s) | 218 |
| Alw26I | GTCTC | 1 cut(s) | 257 |
| AlwI | GGATC | 1 cut(s) | 218 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 64, 96 |
| BccI | CCATC | 3 cut(s) | 46, 212, 260 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 254 |
| BcoDI | GTCTC | 1 cut(s) | 257 |
| BisI | GCNGC | 1 cut(s) | 120 |
| BlsI | GCNGC | 1 cut(s) | 121 |
| BsaBI | GATNNNNATC | 1 cut(s) | 222 |
| Bse8I | GATNNNNATC | 1 cut(s) | 222 |
| BseGI | GGATG | 1 cut(s) | 220 |
| BseJI | GATNNNNATC | 1 cut(s) | 222 |
| BsmAI | GTCTC | 1 cut(s) | 257 |
| Bsp143I | GATC | 1 cut(s) | 223 |
| BspACI | CCGC | 2 cut(s) | 119, 151 |
| BspPI | GGATC | 1 cut(s) | 218 |
| BssMI | GATC | 1 cut(s) | 223 |
| Bst4CI | ACNGT | 1 cut(s) | 7 |
| BstDEI | CTNAG | 2 cut(s) | 75, 276 |
| BstF5I | GGATG | 1 cut(s) | 220 |
| BstKTI | GATC | 1 cut(s) | 226 |
| BstMAI | GTCTC | 1 cut(s) | 257 |
| BstMBI | GATC | 1 cut(s) | 223 |
| BtgZI | GCGATG | 1 cut(s) | 54 |
| BtsCI | GGATG | 1 cut(s) | 220 |
| CviJI | RGCY | 1 cut(s) | 186 |
| CviKI_1 | RGCY | 1 cut(s) | 186 |
| DdeI | CTNAG | 2 cut(s) | 75, 276 |
| DpnI | GATC | 1 cut(s) | 225 |
| DpnII | GATC | 1 cut(s) | 223 |
| FaiI | YATR | 1 cut(s) | 237 |
| FblI | GTMKAC | 1 cut(s) | 131 |
| Fnu4HI | GCNGC | 1 cut(s) | 120 |
| FokI | GGATG | 1 cut(s) | 227 |
| Fsp4HI | GCNGC | 1 cut(s) | 120 |
| GluI | GCNGC | 1 cut(s) | 120 |
| HinfI | GANTC | 1 cut(s) | 47 |
| Hpy166II | GTNNAC | 2 cut(s) | 132, 208 |
| Hpy188I | TCNGA | 1 cut(s) | 97 |
| Hpy188III | TCNNGA | 1 cut(s) | 227 |
| Hpy8I | GTNNAC | 2 cut(s) | 132, 208 |
| HpyAV | CCTTC | 1 cut(s) | 118 |
| HpyCH4III | ACNGT | 1 cut(s) | 7 |
| HpyCH4V | TGCA | 1 cut(s) | 166 |
| HpyF3I | CTNAG | 2 cut(s) | 75, 276 |
| Kzo9I | GATC | 1 cut(s) | 223 |
| LpnPI | CCDG | 1 cut(s) | 183 |
| MalI | GATC | 1 cut(s) | 225 |
| MboI | GATC | 1 cut(s) | 223 |
| MluCI | AATT | 1 cut(s) | 91 |
| MnlI | CCTC | 2 cut(s) | 37, 238 |
| NdeII | GATC | 1 cut(s) | 223 |
| PfeI | GAWTC | 1 cut(s) | 47 |
| PkrI | GCNGC | 1 cut(s) | 121 |
| SatI | GCNGC | 1 cut(s) | 120 |
| Sau3AI | GATC | 1 cut(s) | 223 |
| SetI | ASST | 1 cut(s) | 129 |
| SgeI | CNNG | 7 cut(s) | 44, 74, 82, 182, 223, 239, 256 |
| Sse9I | AATT | 1 cut(s) | 91 |
| SsiI | CCGC | 2 cut(s) | 119, 151 |
| TaaI | ACNGT | 1 cut(s) | 7 |
| TaqI | TCGA | 1 cut(s) | 50 |
| TasI | AATT | 1 cut(s) | 91 |
| TauI | GCSGC | 1 cut(s) | 122 |
| TfiI | GAWTC | 1 cut(s) | 47 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 97 |
| XmiI | GTMKAC | 1 cut(s) | 131 |