Rh2AG407900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001199
Physical Location & Seq
Reverse (-)
60682864 .. 60692626
9763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG407900.1

Sequence Viewer

Length: 474 bp
ATGGAGAAAGGAGAAACCGCCCCAGGCGAAGAGCTTTTCTTTGAGTGCAATGACAACCACTTCACAGCGCTTGAAATCCTCCAGGCTGCAGTCAATAATGAACTCCGTTCGACAAGTAAACTAGGGATAGAGAATTTTCAGCCGTACCCATTCCCAGAGCCCCTAAAACCCTCATGCCACTATGATGGTCAGTTCTTCATGTGGTGGAGACAAATGCGTCAGGAGTATTTCTCCTTGGAGATTCAAGATACGTATGCAATAATCTTTGACTTGCTGTCGGACAAGACTGCTAATGAAACAGCGACGTCCCTTACTTCTGAGGAGCCTTTGAGGAGTCCATTAAATCTTGAGGTGTCAGATGGTGAAGGATCCAAATCCAACTCAGAGGATTATCCATTAATCAGAGACACAGACTCAGAAGATGAAGAGAGTGGTTCCTCCAAAAATGAGGCATATCAAGTTCTCATGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.97

Weight (kDa)

4.18

Isoelectric Point (pI)

65.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 216
AatII GACGTC 1 cut(s) 308
AciI CCGC 1 cut(s) 18
AclWI GGATC 2 cut(s) 363, 376
AcsI RAATTY 1 cut(s) 133
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 1 cut(s) 146
AfeI AGCGCT 1 cut(s) 69
AgsI TTSAA 2 cut(s) 74, 245
AhdI GACNNNNNGTC 1 cut(s) 274
AjnI CCWGG 2 cut(s) 22, 81
AluBI AGCT 1 cut(s) 34
AluI AGCT 1 cut(s) 34
Alw26I GTCTC 2 cut(s) 202, 399
AlwI GGATC 2 cut(s) 363, 376
Aor51HI AGCGCT 1 cut(s) 69
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 133
AseI ATTAAT 1 cut(s) 398
AspLEI GCGC 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 374
BamHI GGATCC 1 cut(s) 368
BanII GRGCYC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 73
BccI CCATC 2 cut(s) 179, 353
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 2 cut(s) 24, 83
BcoDI GTCTC 2 cut(s) 202, 399
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 71
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
Bme1390I CCNGG 2 cut(s) 24, 83
BmeRI GACNNNNNGTC 1 cut(s) 274
BmiI GGNNCC 3 cut(s) 324, 370, 436
BmrFI CCNGG 2 cut(s) 24, 83
BplI GAGNNNNNCTC 2 cut(s) 215, 247
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 368
BsaAI YACGTR 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 22, 234
Bse3DI GCAATG 1 cut(s) 55
Bse8I GATNNNNATC 1 cut(s) 373
BseBI CCWGG 2 cut(s) 24, 83
BseDI CCNNGG 2 cut(s) 22, 234
BseJI GATNNNNATC 1 cut(s) 373
BseMI GCAATG 1 cut(s) 55
BseMII CTCAG 3 cut(s) 309, 396, 429
BseRI GAGGAG 2 cut(s) 335, 346
BseXI GCAGC 1 cut(s) 73
BslFI GGGAC 1 cut(s) 292
BsmAI GTCTC 2 cut(s) 202, 399
BsmFI GGGAC 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 1 cut(s) 368
BspACI CCGC 1 cut(s) 18
BspCNI CTCAG 3 cut(s) 310, 395, 428
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 3 cut(s) 324, 370, 436
BspMAI CTGCAG 1 cut(s) 91
BspPI GGATC 2 cut(s) 363, 376
BspQI GCTCTTC 1 cut(s) 24
BsrDI GCAATG 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 22, 234
BssMI GATC 1 cut(s) 368
BssNI GRCGYC 1 cut(s) 305
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 2 cut(s) 24, 83
Bst6I CTCTTC 2 cut(s) 24, 420
BstACI GRCGYC 1 cut(s) 305
BstBAI YACGTR 1 cut(s) 252
BstDEI CTNAG 3 cut(s) 318, 382, 415
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 1 cut(s) 371
BstMAI GTCTC 2 cut(s) 202, 399
BstMBI GATC 1 cut(s) 368
BstNI CCWGG 2 cut(s) 24, 83
BstSCI CCNGG 2 cut(s) 22, 81
BstSFI CTRYAG 1 cut(s) 87
BstSNI TACGTA 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 73
BstX2I RGATCY 1 cut(s) 368
BstXI CCANNNNNNTGG 1 cut(s) 185
BstYI RGATCY 1 cut(s) 368
CciI TCATGA 1 cut(s) 465
CfoI GCGC 1 cut(s) 70
CseI GACGC 1 cut(s) 206
Csp6I GTAC 1 cut(s) 145
CviAII CATG 3 cut(s) 174, 199, 466
CviJI RGCY 5 cut(s) 34, 86, 142, 160, 325
CviKI_1 RGCY 5 cut(s) 34, 86, 142, 160, 325
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 3 cut(s) 318, 382, 415
DpnI GATC 1 cut(s) 370
DpnII GATC 1 cut(s) 368
DrdI GACNNNNNNGTC 1 cut(s) 216
DriI GACNNNNNGTC 1 cut(s) 274
DseDI GACNNNNNNGTC 1 cut(s) 216
Eam1104I CTCTTC 2 cut(s) 24, 420
Eam1105I GACNNNNNGTC 1 cut(s) 274
EarI CTCTTC 2 cut(s) 24, 420
Eco105I TACGTA 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 162
Eco47III AGCGCT 1 cut(s) 69
EcoRII CCWGG 2 cut(s) 22, 81
EcoT14I CCWWGG 1 cut(s) 234
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 3 cut(s) 177, 202, 469
FaiI YATR 6 cut(s) 175, 183, 200, 255, 454, 467
FaqI GGGAC 1 cut(s) 292
FatI CATG 3 cut(s) 173, 198, 465
Fnu4HI GCNGC 1 cut(s) 87
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 1 cut(s) 122
GlaI GCGC 1 cut(s) 69
GluI GCNGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 65
HaeII RGCGCY 1 cut(s) 71
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 70
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 3 cut(s) 177, 202, 469
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HinfI GANTC 3 cut(s) 241, 334, 413
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 119
Hpy188I TCNGA 6 cut(s) 280, 319, 358, 385, 404, 418
Hpy188III TCNNGA 4 cut(s) 221, 245, 347, 466
Hpy8I GTNNAC 1 cut(s) 119
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 1 cut(s) 359
HpyCH4IV ACGT 2 cut(s) 251, 305
HpyCH4V TGCA 3 cut(s) 48, 89, 257
HpyF3I CTNAG 3 cut(s) 318, 382, 415
HpySE526I ACGT 2 cut(s) 251, 305
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 3 cut(s) 177, 202, 469
HspAI GCGC 1 cut(s) 68
Kzo9I GATC 1 cut(s) 368
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 6 cut(s) 9, 36, 68, 95, 168, 206
Lsp1109I GCAGC 1 cut(s) 73
MaeI CTAG 1 cut(s) 122
MaeII ACGT 2 cut(s) 251, 305
MalI GATC 1 cut(s) 370
MboI GATC 1 cut(s) 368
MboII GAAGA 4 cut(s) 41, 187, 431, 437
MflI RGATCY 1 cut(s) 368
MhlI GDGCHC 1 cut(s) 162
MluCI AATT 1 cut(s) 133
MlyI GAGTC 2 cut(s) 343, 407
MmeI TCCRAC 2 cut(s) 258, 402
MnlI CCTC 8 cut(s) 89, 181, 313, 324, 343, 379, 442, 448
MseI TTAA 2 cut(s) 341, 398
MslI CAYNNNNRTG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 24, 83
MvaI CCWGG 2 cut(s) 24, 83
NdeII GATC 1 cut(s) 368
NlaIII CATG 3 cut(s) 177, 202, 469
NlaIV GGNNCC 3 cut(s) 324, 370, 436
PagI TCATGA 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 1 cut(s) 88
PleI GAGTC 2 cut(s) 342, 407
PpsI GAGTC 2 cut(s) 342, 407
Ppu21I YACGTR 1 cut(s) 252
PshBI ATTAAT 1 cut(s) 398
Psp6I CCWGG 2 cut(s) 22, 81
PspGI CCWGG 2 cut(s) 22, 81
PspN4I GGNNCC 3 cut(s) 324, 370, 436
PstI CTGCAG 1 cut(s) 91
PsuI RGATCY 1 cut(s) 368
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 24
SaqAI TTAA 2 cut(s) 341, 398
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 1 cut(s) 368
SchI GAGTC 2 cut(s) 343, 407
ScrFI CCNGG 2 cut(s) 24, 83
SduI GDGCHC 1 cut(s) 162
SetI ASST 4 cut(s) 36, 254, 308, 354
SfcI CTRYAG 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SnaBI TACGTA 1 cut(s) 252
Sse9I AATT 1 cut(s) 133
SsiI CCGC 1 cut(s) 18
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 2 cut(s) 22, 81
StyI CCWWGG 1 cut(s) 234
TaiI ACGT 2 cut(s) 254, 308
TaqI TCGA 1 cut(s) 110
TasI AATT 1 cut(s) 133
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 2 cut(s) 341, 398
Tru9I TTAA 2 cut(s) 341, 398
TseI GCWGC 1 cut(s) 86
TspDTI ATGAA 4 cut(s) 114, 187, 309, 438
TspGWI ACGGA 1 cut(s) 95
VspI ATTAAT 1 cut(s) 398
XapI RAATTY 1 cut(s) 133
XspI CTAG 1 cut(s) 122
ZraI GACGTC 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.