AT1G21530

ligase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000677
Physical Location & Seq
Reverse (-)
7545149 .. 7546988
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21530.2

Sequence Viewer

Length: 1644 bp
ATGGAACTCTTACTTCCACACCCATCAAACTCAACTCCACTCACCGTCCTCGGCTTCTTAGACCGAGCCGCTTCCGTCTATGGTGACTGTCCGTCCATCCTCCACACTGCAAACACCGTTCATACTTGGTCCGAAACACATAACCGCTGTCTCCGTATCGCATCGGCTCTAACTTCCTCCTCTATCGGAATTAAACAAGGCCAAGTCGTCTCTGTCGTGGGTCCCAACGTCCCGTCCGTCTATGAGCTTCAGTTTGCTGTCCCAATGTCCGGAGCCATCTTAAACAACATCAACCCTCGCTTAGACGCACACGCACTCTCTGTCCTCCTGCGTCACAGCGAATCCAGACTCGTTTTCGTCGACCACCGTTCTATATCCTTAGTCCTTGAAGCAGTTTCATTATTCACACAACACGAGAAACCTCACCTCGTCCTCCTGGACGATGACCAAGAGAACGATTCTTCATCCGCATCAGATTTTCTTGACACGTACGAAGAAATTATGGAGAGAGGAAATTCAAGATTCAAGTGGATCCGTCCTCAAACCGAATGGCAACCAATGGTTCTTAACTATACTTCCGGAACGACGTCGTCTCCCAAGGGAGTGGTACTTAGCCACAGAGCGATTTTCATGCTCACTGTTAGCTCCTTGCTTGATTGGTCAGTACCAAACCGGCCAGTTTACTTGTGGACTCTACCGATGTTTCACGCCAATGGTTGGGGTTACACTTGGGGCACCGCAGCGGTTGGAGCCACCAACATCTGCACGCGTAGAGTCGACGCACCGACTATTTACAACTTGATCGATAAGCACAATGTGACCCACATGTGTGCTGCACCTATGGTTCTCAACATGCTAATTAACTATCCATTAAGTACGCCGCTCAAGAACCCGGTTATGACCTCTGGAGCTCCCCCACCAGCAACCATTATCTCCCGAGCGGAGTCACTTGGTTTCAACGTCAGCCACTCATACGGTTTAACAGAGACTAGCGGTCCGGTTGTGTCATGTGCTTGGAAGCCTAAGTGGGACCATCTTGATCCATTGGAGAGAGCTAGGCTGAAGTCAAGGCAAGGAGTAAGAACACTCGGATTCACGGAAGTCGATGTAAGGGATCGAAAAACAGGAAAGAGTGTGAAACACGACGGAGTTTCGGTTGGAGAGATTGTTTTCAGAGGCAGCTCAGTCATGTTGGGATACTACAAAGACCCTCAAGGAACTGCGGCTTGTATGAGAGAGGACGGGTGGTTCTACTCTGGAGACATCGGGGTTATACACAAAGATGGTTACTTGGAGATCAAAGATCGGTCAAAAGATGTGATCATATGCGGAGGAGAAAATATAAGCAGCGCAGAGATTGAGACGGTTCTGTATACAAATCCGGTGGTGAAGGAAGCTGCGGTGGTGGCTAAACCGGATAAGATGTGGGGAGAGACACCATGTGCTTTTGTGAGCTTGAAGTGTGATAACAATGGTGATGGTTCGGTTCCCGTGACCGAGAGAGAGATAAGGGAGTTTTGTAAGACGAAGTTACCTAAGTACATGGTTCCGAGGAAAGTGATCTTTCAGGAGGAACTTCCCAAGACTTCCACAGGAAAAATTCAGAAGTTTTTGCTAAGACAAATGGCTAAGACCCTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

547

Amino Acids

60.57

Weight (kDa)

7.63

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 403 8.9e-75 AMP-binding enzyme
AMP-binding_C PF13193 454 - 535 1.2e-23 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 590
AccB1I GGYRCC 1 cut(s) 734
AccB7I CCANNNNNTGG 1 cut(s) 717
AccBSI CCGCTC 2 cut(s) 883, 941
AccI GTMKAC 3 cut(s) 360, 777, 1373
AccII CGCG 1 cut(s) 769
AccIII TCCGGA 2 cut(s) 269, 578
AclWI GGATC 4 cut(s) 526, 539, 1034, 1122
AcoI YGGCCR 1 cut(s) 674
AcsI RAATTY 2 cut(s) 514, 1599
AcuI CTGAAG 2 cut(s) 233, 1082
AcyI GRCGYC 1 cut(s) 587
AdeI CACNNNGTG 2 cut(s) 817, 1442
AfaI GTAC 5 cut(s) 491, 609, 666, 877, 1541
AfiI CCNNNNNNNGG 2 cut(s) 269, 717
AflIII ACRYGT 3 cut(s) 486, 767, 825
AgsI TTSAA 5 cut(s) 389, 519, 526, 958, 1459
AhdI GACNNNNNGTC 2 cut(s) 91, 993
AjnI CCWGG 1 cut(s) 435
AleI CACNNNNGTG 1 cut(s) 828
AloI GAACNNNNNNTCC 2 cut(s) 1232, 1264
AluBI AGCT 7 cut(s) 247, 645, 911, 1055, 1182, 1397, 1455
AluI AGCT 7 cut(s) 247, 645, 911, 1055, 1182, 1397, 1455
Alw21I GWGCWC 1 cut(s) 913
Alw26I GTCTC 7 cut(s) 155, 214, 597, 980, 1254, 1355, 1427
AlwI GGATC 4 cut(s) 526, 539, 1034, 1122
Ama87I CYCGRG 1 cut(s) 936
Aor13HI TCCGGA 2 cut(s) 269, 578
AoxI GGCC 2 cut(s) 199, 674
ApeKI GCWGC 5 cut(s) 740, 833, 1179, 1347, 1397
ApoI RAATTY 2 cut(s) 514, 1599
AspLEI GCGC 1 cut(s) 1352
AspS9I GGNCC 4 cut(s) 129, 221, 995, 1030
AsuC2I CCSGG 1 cut(s) 893
AsuHPI GGTGA 5 cut(s) 34, 95, 416, 1399, 1487
AvaI CYCGRG 1 cut(s) 936
AvaII GGWCC 4 cut(s) 129, 221, 995, 1030
BaeGI GKGCMC 1 cut(s) 737
BamHI GGATCC 1 cut(s) 531
BanI GGYRCC 1 cut(s) 734
BanII GRGCYC 1 cut(s) 913
BauI CACGAG 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 913
BbvI GCAGC 5 cut(s) 752, 820, 1191, 1359, 1384
BccI CCATC 6 cut(s) 31, 104, 284, 1041, 1277, 1472
BcgI CGANNNNNNTGC 2 cut(s) 613, 647
BciT130I CCWGG 1 cut(s) 437
BciVI GTATCC 1 cut(s) 1190
BclI TGATCA 1 cut(s) 1320
BcnI CCSGG 1 cut(s) 893
BcoDI GTCTC 7 cut(s) 155, 214, 597, 980, 1254, 1355, 1427
BfaI CTAG 2 cut(s) 990, 1056
BfuI GTATCC 1 cut(s) 1190
BisI GCNGC 8 cut(s) 69, 741, 834, 881, 1180, 1224, 1348, 1398
BlsI GCNGC 8 cut(s) 70, 742, 835, 882, 1181, 1225, 1349, 1399
Bme1390I CCNGG 2 cut(s) 437, 893
Bme18I GGWCC 4 cut(s) 129, 221, 995, 1030
BmeRI GACNNNNNGTC 2 cut(s) 91, 993
BmeT110I CYCGRG 1 cut(s) 936
BmgT120I GGNCC 4 cut(s) 129, 221, 995, 1030
BmiI GGNNCC 9 cut(s) 222, 223, 274, 533, 736, 751, 1031, 1488, 1548
BmrFI CCNGG 2 cut(s) 437, 893
BmsI GCATC 2 cut(s) 170, 479
BpmI CTGGAG 2 cut(s) 927, 1278
BpuEI CTTGAG 2 cut(s) 869, 1197
BpuMI CCSGG 1 cut(s) 893
Bsa29I ATCGAT 1 cut(s) 804
BsaAI YACGTR 1 cut(s) 489
BsaHI GRCGYC 1 cut(s) 587
BsaJI CCNNGG 3 cut(s) 49, 597, 1550
BsaWI WCCGGW 5 cut(s) 269, 578, 997, 1381, 1414
BsaXI ACNNNNNCTCC 6 cut(s) 577, 607, 1140, 1152, 1170, 1182
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 717
Bse118I RCCGGY 1 cut(s) 672
Bse1I ACTGG 1 cut(s) 677
BseAI TCCGGA 2 cut(s) 269, 578
BseBI CCWGG 1 cut(s) 437
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 3 cut(s) 49, 597, 1550
BseGI GGATG 2 cut(s) 96, 464
BseLI CCNNNNNNNGG 2 cut(s) 269, 717
BseMII CTCAG 1 cut(s) 1197
BseNI ACTGG 1 cut(s) 677
BseRI GAGGAG 2 cut(s) 169, 1347
BseSI GKGCMC 1 cut(s) 737
BseXI GCAGC 5 cut(s) 752, 820, 1191, 1359, 1384
BsgI GTGCAG 2 cut(s) 748, 819
Bsh1236I CGCG 1 cut(s) 769
BshFI GGCC 2 cut(s) 201, 676
BshNI GGYRCC 1 cut(s) 734
BshVI ATCGAT 1 cut(s) 804
BsiHKAI GWGCWC 1 cut(s) 913
BsiHKCI CYCGRG 1 cut(s) 936
BsiSI CCGG 7 cut(s) 270, 579, 673, 893, 998, 1382, 1415
BsiWI CGTACG 1 cut(s) 489
BslFI GGGAC 4 cut(s) 207, 215, 245, 1043
BslI CCNNNNNNNGG 2 cut(s) 269, 717
BsmAI GTCTC 7 cut(s) 155, 214, 597, 980, 1254, 1355, 1427
BsmBI CGTCTC 3 cut(s) 214, 597, 1355
BsmFI GGGAC 4 cut(s) 207, 215, 245, 1043
BsnI GGCC 2 cut(s) 201, 676
BsoBI CYCGRG 1 cut(s) 936
Bsp1286I GDGCHC 2 cut(s) 737, 913
Bsp13I TCCGGA 2 cut(s) 269, 578
Bsp143I GATC 8 cut(s) 531, 801, 1039, 1114, 1296, 1303, 1320, 1560
BspANI GGCC 2 cut(s) 201, 676
BspCNI CTCAG 1 cut(s) 1196
BspDI ATCGAT 1 cut(s) 804
BspEI TCCGGA 2 cut(s) 269, 578
BspFNI CGCG 1 cut(s) 769
BspLI GGNNCC 9 cut(s) 222, 223, 274, 533, 736, 751, 1031, 1488, 1548
BspPI GGATC 4 cut(s) 526, 539, 1034, 1122
BspT107I GGYRCC 1 cut(s) 734
BsrBI CCGCTC 2 cut(s) 883, 941
BsrFI RCCGGY 1 cut(s) 672
BsrI ACTGG 1 cut(s) 677
BssAI RCCGGY 1 cut(s) 672
BssECI CCNNGG 3 cut(s) 49, 597, 1550
BssMI GATC 8 cut(s) 531, 801, 1039, 1114, 1296, 1303, 1320, 1560
BssNAI GTATAC 1 cut(s) 1374
BssNI GRCGYC 1 cut(s) 587
BssSI CACGAG 1 cut(s) 413
BssT1I CCWWGG 1 cut(s) 597
Bst1107I GTATAC 1 cut(s) 1374
Bst2BI CACGAG 1 cut(s) 413
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 7 cut(s) 46, 89, 118, 368, 640, 977, 1366
BstACI GRCGYC 1 cut(s) 587
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 1 cut(s) 767
BstDEI CTNAG 9 cut(s) 58, 301, 379, 611, 1023, 1183, 1536, 1616, 1629
BstF5I GGATG 2 cut(s) 96, 464
BstFNI CGCG 1 cut(s) 769
BstHHI GCGC 1 cut(s) 1352
BstKTI GATC 8 cut(s) 534, 804, 1042, 1117, 1299, 1306, 1323, 1563
BstMAI GTCTC 7 cut(s) 155, 214, 597, 980, 1254, 1355, 1427
BstMBI GATC 8 cut(s) 531, 801, 1039, 1114, 1296, 1303, 1320, 1560
BstMWI GCNNNNNNNGC 2 cut(s) 749, 1406
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 2 cut(s) 829, 856
BstSCI CCNGG 2 cut(s) 435, 891
BstSLI GKGCMC 1 cut(s) 737
BstUI CGCG 1 cut(s) 769
BstV1I GCAGC 5 cut(s) 752, 820, 1191, 1359, 1384
BstX2I RGATCY 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 1 cut(s) 531
BstZ17I GTATAC 1 cut(s) 1374
Bsu15I ATCGAT 1 cut(s) 804
BsuI GTATCC 1 cut(s) 1190
BsuRI GGCC 2 cut(s) 201, 676
BsuTUI ATCGAT 1 cut(s) 804
BtsCI GGATG 2 cut(s) 96, 464
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 636
Cac8I GCNNGC 1 cut(s) 767
CfoI GCGC 1 cut(s) 1352
Cfr10I RCCGGY 1 cut(s) 672
Cfr13I GGNCC 4 cut(s) 129, 221, 995, 1030
ClaI ATCGAT 1 cut(s) 804
CpoI CGGWCCG 1 cut(s) 995
CseI GACGC 3 cut(s) 314, 320, 788
Csp6I GTAC 5 cut(s) 490, 608, 665, 876, 1540
CspCI CAANNNNNGTGG 2 cut(s) 1365, 1400
CspI CGGWCCG 1 cut(s) 995
CviAII CATG 7 cut(s) 631, 826, 853, 1008, 1189, 1440, 1543
CviQI GTAC 5 cut(s) 490, 608, 665, 876, 1540
DdeI CTNAG 9 cut(s) 58, 301, 379, 611, 1023, 1183, 1536, 1616, 1629
DpnI GATC 8 cut(s) 533, 803, 1041, 1116, 1298, 1305, 1322, 1562
DpnII GATC 8 cut(s) 531, 801, 1039, 1114, 1296, 1303, 1320, 1560
DraIII CACNNNGTG 2 cut(s) 817, 1442
DriI GACNNNNNGTC 2 cut(s) 91, 993
EaeI YGGCCR 1 cut(s) 674
Eam1105I GACNNNNNGTC 2 cut(s) 91, 993
Ecl136II GAGCTC 1 cut(s) 911
Eco130I CCWWGG 1 cut(s) 597
Eco24I GRGCYC 1 cut(s) 913
Eco47I GGWCC 4 cut(s) 129, 221, 995, 1030
Eco53kI GAGCTC 1 cut(s) 911
Eco57I CTGAAG 2 cut(s) 233, 1082
Eco88I CYCGRG 1 cut(s) 936
EcoICRI GAGCTC 1 cut(s) 911
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 597
EcoT38I GRGCYC 1 cut(s) 913
ErhI CCWWGG 1 cut(s) 597
Esp3I CGTCTC 3 cut(s) 214, 597, 1355
FaeI CATG 7 cut(s) 634, 829, 856, 1011, 1192, 1443, 1546
FaqI GGGAC 4 cut(s) 207, 215, 245, 1043
FatI CATG 7 cut(s) 630, 825, 852, 1007, 1188, 1439, 1542
FauNDI CATATG 1 cut(s) 1325
FbaI TGATCA 1 cut(s) 1320
FblI GTMKAC 3 cut(s) 360, 777, 1373
Fnu4HI GCNGC 8 cut(s) 69, 741, 834, 881, 1180, 1224, 1348, 1398
FokI GGATG 2 cut(s) 83, 451
FriOI GRGCYC 1 cut(s) 913
Fsp4HI GCNGC 8 cut(s) 69, 741, 834, 881, 1180, 1224, 1348, 1398
FspBI CTAG 2 cut(s) 990, 1056
GlaI GCGC 1 cut(s) 1351
GluI GCNGC 8 cut(s) 69, 741, 834, 881, 1180, 1224, 1348, 1398
GsuI CTGGAG 2 cut(s) 927, 1278
HaeIII GGCC 2 cut(s) 201, 676
HapII CCGG 7 cut(s) 270, 579, 673, 893, 998, 1382, 1415
HgaI GACGC 3 cut(s) 314, 320, 788
HhaI GCGC 1 cut(s) 1352
Hin1I GRCGYC 1 cut(s) 587
Hin1II CATG 7 cut(s) 634, 829, 856, 1011, 1192, 1443, 1546
Hin6I GCGC 1 cut(s) 1350
HinP1I GCGC 1 cut(s) 1350
HincII GTYRAC 2 cut(s) 361, 778
HindII GTYRAC 2 cut(s) 361, 778
HinfI GANTC 8 cut(s) 341, 348, 458, 522, 691, 774, 944, 1092
HpaII CCGG 7 cut(s) 270, 579, 673, 893, 998, 1382, 1415
HphI GGTGA 5 cut(s) 34, 95, 416, 1399, 1487
Hpy166II GTNNAC 5 cut(s) 361, 682, 690, 778, 1374
Hpy188I TCNGA 7 cut(s) 133, 188, 475, 1091, 1175, 1551, 1605
Hpy8I GTNNAC 5 cut(s) 361, 682, 690, 778, 1374
Hpy99I CGWCG 5 cut(s) 362, 589, 592, 782, 1148
HpyAV CCTTC 1 cut(s) 1384
HpyCH4III ACNGT 7 cut(s) 46, 89, 118, 368, 640, 977, 1366
HpyCH4IV ACGT 4 cut(s) 228, 488, 587, 960
HpyCH4V TGCA 3 cut(s) 110, 765, 836
HpyF10VI GCNNNNNNNGC 2 cut(s) 749, 1406
HpyF3I CTNAG 9 cut(s) 58, 301, 379, 611, 1023, 1183, 1536, 1616, 1629
HpySE526I ACGT 4 cut(s) 228, 488, 587, 960
Hsp92I GRCGYC 1 cut(s) 587
Hsp92II CATG 7 cut(s) 634, 829, 856, 1011, 1192, 1443, 1546
HspAI GCGC 1 cut(s) 1350
KflI GGGWCCC 1 cut(s) 221
Kpn2I TCCGGA 2 cut(s) 269, 578
Ksp22I TGATCA 1 cut(s) 1320
Kzo9I GATC 8 cut(s) 531, 801, 1039, 1114, 1296, 1303, 1320, 1560
LmnI GCTCC 5 cut(s) 272, 650, 749, 908, 916
Lsp1109I GCAGC 5 cut(s) 752, 820, 1191, 1359, 1384
LweI GCATC 2 cut(s) 170, 479
MaeI CTAG 2 cut(s) 990, 1056
MaeII ACGT 4 cut(s) 228, 488, 587, 960
MaeIII GTNAC 8 cut(s) 83, 332, 722, 817, 945, 1286, 1492, 1530
MalI GATC 8 cut(s) 533, 803, 1041, 1116, 1298, 1305, 1322, 1562
MbiI CCGCTC 2 cut(s) 883, 941
MboI GATC 8 cut(s) 531, 801, 1039, 1114, 1296, 1303, 1320, 1560
MboII GAAGA 2 cut(s) 453, 506
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 2 cut(s) 737, 913
MluCI AATT 5 cut(s) 189, 498, 514, 858, 1599
MluI ACGCGT 1 cut(s) 767
MlyI GAGTC 4 cut(s) 342, 685, 783, 953
MmeI TCCRAC 2 cut(s) 727, 1138
MroI TCCGGA 2 cut(s) 269, 578
MseI TTAA 6 cut(s) 192, 281, 567, 861, 872, 980
MslI CAYNNNNRTG 3 cut(s) 711, 828, 1281
MspA1I CMGCKG 2 cut(s) 147, 743
MspI CCGG 7 cut(s) 270, 579, 673, 893, 998, 1382, 1415
MspR9I CCNGG 2 cut(s) 437, 893
MvaI CCWGG 1 cut(s) 437
MvnI CGCG 1 cut(s) 769
MwoI GCNNNNNNNGC 2 cut(s) 749, 1406
NciI CCSGG 1 cut(s) 893
NdeI CATATG 1 cut(s) 1325
NdeII GATC 8 cut(s) 531, 801, 1039, 1114, 1296, 1303, 1320, 1560
NlaIII CATG 7 cut(s) 634, 829, 856, 1011, 1192, 1443, 1546
NlaIV GGNNCC 9 cut(s) 222, 223, 274, 533, 736, 751, 1031, 1488, 1548
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 5 cut(s) 83, 332, 817, 945, 1492
NspI RCATGY 2 cut(s) 829, 856
OliI CACNNNNGTG 1 cut(s) 828
PciI ACATGT 1 cut(s) 825
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 234, 357
PfeI GAWTC 4 cut(s) 341, 458, 522, 1092
Pfl23II CGTACG 1 cut(s) 489
PflFI GACNNNGTC 2 cut(s) 589, 1636
PflMI CCANNNNNTGG 1 cut(s) 717
PfoI TCCNGGA 1 cut(s) 435
PkrI GCNGC 8 cut(s) 70, 742, 835, 882, 1181, 1225, 1349, 1399
PleI GAGTC 4 cut(s) 342, 685, 782, 952
PpsI GAGTC 4 cut(s) 342, 685, 782, 952
Ppu21I YACGTR 1 cut(s) 489
PpuMI RGGWCCY 1 cut(s) 221
PscI ACATGT 1 cut(s) 825
Psp124BI GAGCTC 1 cut(s) 913
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspLI CGTACG 1 cut(s) 489
PspN4I GGNNCC 9 cut(s) 222, 223, 274, 533, 736, 751, 1031, 1488, 1548
PspPI GGNCC 4 cut(s) 129, 221, 995, 1030
PspPPI RGGWCCY 1 cut(s) 221
PsuI RGATCY 1 cut(s) 531
PsyI GACNNNGTC 2 cut(s) 589, 1636
RsaI GTAC 5 cut(s) 491, 609, 666, 877, 1541
RsaNI GTAC 5 cut(s) 490, 608, 665, 876, 1540
RseI CAYNNNNRTG 3 cut(s) 711, 828, 1281
Rsr2I CGGWCCG 1 cut(s) 995
RsrII CGGWCCG 1 cut(s) 995
SacI GAGCTC 1 cut(s) 913
SalI GTCGAC 2 cut(s) 359, 776
SaqAI TTAA 6 cut(s) 192, 281, 567, 861, 872, 980
SatI GCNGC 8 cut(s) 69, 741, 834, 881, 1180, 1224, 1348, 1398
Sau3AI GATC 8 cut(s) 531, 801, 1039, 1114, 1296, 1303, 1320, 1560
Sau96I GGNCC 4 cut(s) 129, 221, 995, 1030
SchI GAGTC 4 cut(s) 342, 685, 783, 953
ScrFI CCNGG 2 cut(s) 437, 893
SduI GDGCHC 2 cut(s) 737, 913
SfaNI GCATC 2 cut(s) 170, 479
SinI GGWCC 4 cut(s) 129, 221, 995, 1030
SmiMI CAYNNNNRTG 3 cut(s) 711, 828, 1281
SmlI CTYRAG 2 cut(s) 884, 1212
SmoI CTYRAG 2 cut(s) 884, 1212
Sse9I AATT 5 cut(s) 189, 498, 514, 858, 1599
SspMI CTAG 2 cut(s) 990, 1056
SstI GAGCTC 1 cut(s) 913
StyD4I CCNGG 2 cut(s) 435, 891
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 7 cut(s) 46, 89, 118, 368, 640, 977, 1366
TaiI ACGT 4 cut(s) 231, 491, 590, 963
TaqI TCGA 5 cut(s) 360, 777, 804, 1104, 1117
TaqII GACCGA 3 cut(s) 78, 1296, 1511
TasI AATT 5 cut(s) 189, 498, 514, 858, 1599
TatI WGTACW 1 cut(s) 1539
TauI GCSGC 3 cut(s) 71, 883, 1226
TfiI GAWTC 4 cut(s) 341, 458, 522, 1092
Tru1I TTAA 6 cut(s) 192, 281, 567, 861, 872, 980
Tru9I TTAA 6 cut(s) 192, 281, 567, 861, 872, 980
TscAI CASTG 2 cut(s) 112, 643
TseFI GTSAC 5 cut(s) 83, 332, 817, 945, 1492
TseI GCWGC 5 cut(s) 740, 833, 1179, 1347, 1397
Tsp45I GTSAC 5 cut(s) 83, 332, 817, 945, 1492
TspDTI ATGAA 4 cut(s) 110, 387, 453, 619
TspGWI ACGGA 7 cut(s) 64, 81, 143, 226, 524, 1112, 1161
TspRI CASTG 2 cut(s) 112, 643
Tth111I GACNNNGTC 2 cut(s) 589, 1636
Van91I CCANNNNNTGG 1 cut(s) 717
VpaK11BI GGWCC 4 cut(s) 129, 221, 995, 1030
XapI RAATTY 2 cut(s) 514, 1599
XceI RCATGY 2 cut(s) 829, 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 684
XmiI GTMKAC 3 cut(s) 360, 777, 1373
XspI CTAG 2 cut(s) 990, 1056
ZraI GACGTC 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.