Rh3CG319200

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000677
Physical Location & Seq
Reverse (-)
34165501 .. 34183855
18355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG319200.1

Sequence Viewer

Length: 1035 bp
ATGTTGGGTTATCTGAAAGACCCAAAAGGCACGGCCAGTTGCATGAAAGACGGTTGGTTTTACACCGGCGACGTGGCGGTGATGCACCCGGACGGGTATCTGGAGATCAAAGACAGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACTCGGCGGTGAACGAGGCTGCCGTGGTGGCGAGGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGATGACGTGGGGCGGAGGCCGACGGAGAAGGAGATGATAGAGTACTGCAGAGGAAAGCTGCCGAATTTCATGGTGCCAAAGACGGTGGTGTTTATTGAGGAGCTGCCGAAGACGTCGACGGGGAAAATTCAGAAGTTCGTGCTGAGAGAGATGGCGGCCCAGTGTAACTTGATTCGCGGCTCGTTGCGGAACGGATCAGAGAGTTTTGATGCAGGACTTCAAGTTCCTCAATCTGCTGTGACATCGTTTTTCGAAGAGCAATCTTCTGAACTCCAAACTTTTGAGTTCTTATCAAGTTCCACCAGTGAGCATTCCGAGGAATTGACATTGAGGAGAACTGGGATAAGGTTAAGACCATATTTGTCCCAACGGGAGCTTCCCTTTCACCAGATGTATCTGCTCCACTTGTTGCAACTCTTCCCTTCACTACCAGAAAAAAGTCTGTATTTGCTCTATTTCAAGTGTGCAACTTCTGATCCTGATGACCGTAAGAGAGCCCTTGTAATTGGAGGAGGAATTGCTAACTTCACTGATGTAGCTGCAACATTCAATGGCATTATTAGAGCCTTGAAGGAGAAGGAAACAAAGCTAAAGGCTGATAGGATGCATCTTTATGTTAGGAGAGGAGGTCCCAACTACCAGAGGGGACTCGCAAAAATGAGGGCACTTGCAGAGGAAATTGGCCTCCCTATTGAGGTCTATGGTCCTGAAGCAACAATGACTGGTATTTGCAAGCAGCTGGCAATCCAGTGCATCACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

344

Amino Acids

38.49

Weight (kDa)

6.28

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 55 - 132 1.4e-24 AMP-binding enzyme C-terminal domain
Citrate_bind PF16114 244 - 336 1e-35 ATP citrate lyase citrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 386
AccII CGCG 2 cut(s) 254, 447
AciI CCGC 7 cut(s) 77, 138, 194, 283, 425, 447, 457
AclWI GGATC 3 cut(s) 111, 472, 740
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 2 cut(s) 334, 396
AcuI CTGAAG 1 cut(s) 999
AcyI GRCGYC 2 cut(s) 245, 383
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 964
AgsI TTSAA 4 cut(s) 491, 730, 820, 841
AjiI CACGTC 3 cut(s) 73, 127, 277
AjuI GAANNNNNNNTTGG 2 cut(s) 630, 662
AloI GAACNNNNNNTCC 2 cut(s) 477, 509
AluBI AGCT 6 cut(s) 328, 373, 646, 809, 859, 1009
AluI AGCT 6 cut(s) 328, 373, 646, 809, 859, 1009
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 3 cut(s) 111, 472, 740
AoxI GGCC 5 cut(s) 33, 221, 287, 426, 952
ApeKI GCWGC 6 cut(s) 156, 206, 328, 373, 809, 1006
ApoI RAATTY 2 cut(s) 334, 396
AspLEI GCGC 1 cut(s) 254
AspS9I GGNCC 3 cut(s) 427, 899, 974
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 3 cut(s) 91, 208, 647
AsuII TTCGAA 1 cut(s) 522
AvaII GGWCC 2 cut(s) 899, 974
BaeGI GKGCMC 1 cut(s) 937
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 769
BbsI GAAGAC 1 cut(s) 386
BbvI GCAGC 6 cut(s) 168, 193, 315, 360, 796, 1018
BccI CCATC 1 cut(s) 415
BceAI ACGGC 3 cut(s) 48, 194, 232
BclI TGATCA 1 cut(s) 129
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 215
BisI GCNGC 8 cut(s) 157, 207, 329, 374, 426, 448, 810, 1007
BlsI GCNGC 8 cut(s) 158, 208, 330, 375, 427, 449, 811, 1008
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 2 cut(s) 899, 974
BmgBI CACGTC 3 cut(s) 73, 127, 277
BmgT120I GGNCC 3 cut(s) 427, 899, 974
BmiI GGNNCC 2 cut(s) 345, 901
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 2 cut(s) 424, 618
BmsI GCATC 4 cut(s) 72, 469, 864, 886
BmuI ACTGGG 2 cut(s) 424, 618
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 122
Bpu14I TTCGAA 1 cut(s) 522
BpuEI CTTGAG 1 cut(s) 172
BpuMI CCSGG 1 cut(s) 89
BsaHI GRCGYC 2 cut(s) 245, 383
BsaJI CCNNGG 3 cut(s) 120, 210, 585
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 233, 263, 883, 913
Bsc4I CCNNNNNNNGG 1 cut(s) 964
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 6 cut(s) 36, 430, 573, 613, 997, 1018
BseDI CCNNGG 3 cut(s) 120, 210, 585
BseGI GGATG 1 cut(s) 879
BseLI CCNNNNNNNGG 1 cut(s) 964
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 6 cut(s) 36, 430, 573, 613, 997, 1018
BseRI GAGGAG 4 cut(s) 383, 616, 795, 909
BseSI GKGCMC 1 cut(s) 937
BseXI GCAGC 6 cut(s) 168, 193, 315, 360, 796, 1018
Bsh1236I CGCG 2 cut(s) 254, 447
BshFI GGCC 5 cut(s) 35, 223, 289, 428, 954
BshNI GGYRCC 1 cut(s) 343
BsiSI CCGG 2 cut(s) 66, 89
BslFI GGGAC 3 cut(s) 619, 885, 930
BslI CCNNNNNNNGG 1 cut(s) 964
BsmAI GTCTC 1 cut(s) 236
BsmBI CGTCTC 1 cut(s) 236
BsmFI GGGAC 3 cut(s) 619, 885, 930
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 5 cut(s) 35, 223, 289, 428, 954
Bsp119I TTCGAA 1 cut(s) 522
Bsp1286I GDGCHC 2 cut(s) 769, 937
Bsp143I GATC 5 cut(s) 105, 116, 129, 464, 745
BspACI CCGC 7 cut(s) 77, 138, 194, 283, 425, 447, 457
BspANI GGCC 5 cut(s) 35, 223, 289, 428, 954
BspCNI CTCAG 1 cut(s) 405
BspFNI CGCG 2 cut(s) 254, 447
BspLI GGNNCC 2 cut(s) 345, 901
BspMAI CTGCAG 1 cut(s) 320
BspPI GGATC 3 cut(s) 111, 472, 740
BspQI GCTCTTC 1 cut(s) 519
BspT104I TTCGAA 1 cut(s) 522
BspT107I GGYRCC 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 6 cut(s) 36, 430, 573, 613, 997, 1018
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 3 cut(s) 120, 210, 585
BssMI GATC 5 cut(s) 105, 116, 129, 464, 745
BssNI GRCGYC 2 cut(s) 245, 383
BssT1I CCWWGG 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 53, 355, 758
Bst6I CTCTTC 2 cut(s) 519, 692
BstACI GRCGYC 2 cut(s) 245, 383
BstBI TTCGAA 1 cut(s) 522
BstC8I GCNNGC 2 cut(s) 1004, 1011
BstDEI CTNAG 1 cut(s) 413
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 879
BstFNI CGCG 2 cut(s) 254, 447
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 5 cut(s) 108, 119, 132, 467, 748
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 5 cut(s) 105, 116, 129, 464, 745
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 316
BstSLI GKGCMC 1 cut(s) 937
BstUI CGCG 2 cut(s) 254, 447
BstV1I GCAGC 6 cut(s) 168, 193, 315, 360, 796, 1018
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BsuRI GGCC 5 cut(s) 35, 223, 289, 428, 954
BtgI CCRYGG 1 cut(s) 210
BtrI CACGTC 3 cut(s) 73, 127, 277
BtsCI GGATG 1 cut(s) 879
BtsI GCAGTG 1 cut(s) 1026
BtsIMutI CAGTG 5 cut(s) 437, 580, 798, 1025, 1026
Cac8I GCNNGC 2 cut(s) 1004, 1011
CfoI GCGC 1 cut(s) 254
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 427, 899, 974
CseI GACGC 1 cut(s) 253
Csp6I GTAC 1 cut(s) 313
CspCI CAANNNNNGTGG 2 cut(s) 336, 371
CviAII CATG 3 cut(s) 43, 340, 1032
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 1 cut(s) 413
DpnI GATC 5 cut(s) 107, 118, 131, 466, 747
DpnII GATC 5 cut(s) 105, 116, 129, 464, 745
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 2 cut(s) 519, 692
EarI CTCTTC 2 cut(s) 519, 692
EciI GGCGGA 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 120
Eco147I AGGCCT 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 769
Eco47I GGWCC 2 cut(s) 899, 974
Eco57I CTGAAG 1 cut(s) 999
EcoO109I RGGNCCY 1 cut(s) 899
EcoT14I CCWWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 879
EcoT38I GRGCYC 1 cut(s) 769
ErhI CCWWGG 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 3 cut(s) 46, 343, 1035
FaiI YATR 7 cut(s) 44, 134, 341, 628, 885, 972, 1033
FaqI GGGAC 3 cut(s) 619, 885, 930
FatI CATG 3 cut(s) 42, 339, 1031
FauI CCCGC 1 cut(s) 131
FbaI TGATCA 1 cut(s) 129
FblI GTMKAC 1 cut(s) 386
Fnu4HI GCNGC 8 cut(s) 157, 207, 329, 374, 426, 448, 810, 1007
FokI GGATG 1 cut(s) 886
FriOI GRGCYC 1 cut(s) 769
Fsp4HI GCNGC 8 cut(s) 157, 207, 329, 374, 426, 448, 810, 1007
GlaI GCGC 1 cut(s) 253
GluI GCNGC 8 cut(s) 157, 207, 329, 374, 426, 448, 810, 1007
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 5 cut(s) 35, 223, 289, 428, 954
HapII CCGG 2 cut(s) 66, 89
HgaI GACGC 1 cut(s) 253
HhaI GCGC 1 cut(s) 254
Hin1I GRCGYC 2 cut(s) 245, 383
Hin1II CATG 3 cut(s) 46, 343, 1035
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HinfI GANTC 3 cut(s) 170, 442, 918
HpaII CCGG 2 cut(s) 66, 89
HphI GGTGA 3 cut(s) 91, 208, 647
Hpy166II GTNNAC 2 cut(s) 199, 387
Hpy188I TCNGA 6 cut(s) 15, 402, 469, 538, 586, 745
Hpy188III TCNNGA 3 cut(s) 101, 749, 977
Hpy8I GTNNAC 2 cut(s) 199, 387
Hpy99I CGWCG 4 cut(s) 74, 295, 388, 391
HpyAV CCTTC 4 cut(s) 292, 702, 835, 841
HpyCH4III ACNGT 3 cut(s) 53, 355, 758
HpyCH4IV ACGT 4 cut(s) 72, 126, 276, 383
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 1 cut(s) 413
HpySE526I ACGT 4 cut(s) 72, 126, 276, 383
Hsp92I GRCGYC 2 cut(s) 245, 383
Hsp92II CATG 3 cut(s) 46, 343, 1035
HspAI GCGC 1 cut(s) 252
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 5 cut(s) 105, 116, 129, 464, 745
LguI GCTCTTC 1 cut(s) 519
LmnI GCTCC 3 cut(s) 370, 643, 675
Lsp1109I GCAGC 6 cut(s) 168, 193, 315, 360, 796, 1018
LweI GCATC 4 cut(s) 72, 469, 864, 886
MaeII ACGT 4 cut(s) 72, 126, 276, 383
MaeIII GTNAC 2 cut(s) 434, 508
MalI GATC 5 cut(s) 107, 118, 131, 466, 747
MboI GATC 5 cut(s) 105, 116, 129, 464, 745
MboII GAAGA 4 cut(s) 391, 525, 536, 679
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 2 cut(s) 769, 937
MluCI AATT 6 cut(s) 334, 396, 590, 774, 786, 948
MlyI GAGTC 2 cut(s) 179, 912
Mph1103I ATGCAT 1 cut(s) 879
MseI TTAA 1 cut(s) 620
MslI CAYNNNNRTG 1 cut(s) 882
MspA1I CMGCKG 1 cut(s) 1009
MspI CCGG 2 cut(s) 66, 89
MspR9I CCNGG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 580
MvnI CGCG 2 cut(s) 254, 447
MwoI GCNNNNNNNGC 1 cut(s) 215
NciI CCSGG 1 cut(s) 89
NdeII GATC 5 cut(s) 105, 116, 129, 464, 745
NlaIII CATG 3 cut(s) 46, 343, 1035
NlaIV GGNNCC 2 cut(s) 345, 901
NmeAIII GCCGAG 1 cut(s) 170
NmuCI GTSAC 1 cut(s) 508
NsiI ATGCAT 1 cut(s) 879
NspV TTCGAA 1 cut(s) 522
PceI AGGCCT 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 519
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 251
PctI GAATGC 1 cut(s) 580
PfeI GAWTC 1 cut(s) 442
PkrI GCNGC 8 cut(s) 158, 208, 330, 375, 427, 449, 811, 1008
PleI GAGTC 2 cut(s) 178, 912
PpsI GAGTC 2 cut(s) 178, 912
PpuMI RGGWCCY 1 cut(s) 899
Psp5II RGGWCCY 1 cut(s) 899
PspN4I GGNNCC 2 cut(s) 345, 901
PspPI GGNCC 3 cut(s) 427, 899, 974
PspPPI RGGWCCY 1 cut(s) 899
PstI CTGCAG 1 cut(s) 320
PsuI RGATCY 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 1009
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 882
SalI GTCGAC 1 cut(s) 385
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 1 cut(s) 620
SatI GCNGC 8 cut(s) 157, 207, 329, 374, 426, 448, 810, 1007
Sau3AI GATC 5 cut(s) 105, 116, 129, 464, 745
Sau96I GGNCC 3 cut(s) 427, 899, 974
ScaI AGTACT 1 cut(s) 314
SchI GAGTC 2 cut(s) 179, 912
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 2 cut(s) 769, 937
SfaNI GCATC 4 cut(s) 72, 469, 864, 886
SfcI CTRYAG 1 cut(s) 316
SfuI TTCGAA 1 cut(s) 522
SgrAI CRCCGGYG 1 cut(s) 65
SgrDI CGTCGACG 1 cut(s) 385
SinI GGWCC 2 cut(s) 899, 974
SmiMI CAYNNNNRTG 1 cut(s) 882
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
Sse9I AATT 6 cut(s) 334, 396, 590, 774, 786, 948
SseBI AGGCCT 1 cut(s) 223
SsiI CCGC 7 cut(s) 77, 138, 194, 283, 425, 447, 457
StuI AGGCCT 1 cut(s) 223
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 3 cut(s) 53, 355, 758
TaiI ACGT 4 cut(s) 75, 129, 279, 386
TaqI TCGA 2 cut(s) 386, 522
TasI AATT 6 cut(s) 334, 396, 590, 774, 786, 948
TatI WGTACW 1 cut(s) 312
TauI GCSGC 2 cut(s) 428, 450
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 1 cut(s) 620
Tru9I TTAA 1 cut(s) 620
TscAI CASTG 5 cut(s) 437, 580, 805, 1025, 1033
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 6 cut(s) 156, 206, 328, 373, 809, 1006
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 2 cut(s) 59, 328
TspGWI ACGGA 2 cut(s) 308, 477
TspRI CASTG 5 cut(s) 437, 580, 805, 1025, 1033
VpaK11BI GGWCC 2 cut(s) 899, 974
XapI RAATTY 2 cut(s) 334, 396
XmiI GTMKAC 1 cut(s) 386
ZraI GACGTC 1 cut(s) 384
ZrmI AGTACT 1 cut(s) 314
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.