MD17G1273900.v1.1

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000677
Physical Location & Seq
Forward (+)
33560596 .. 33562887
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1273900.v1.1.491

Sequence Viewer

Length: 1839 bp
ATGCAACACCAACAAGCTTACGTTATCTCTACATATATAAAAGTATATAACCAGCACCACCCTCACACACCCACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTCATCGTCAGTTAATTACGTTAAACTAGAAATGGAGCAGCTCAAACCAAGTCCTGCAAATCTCTGCCCTCTCACTCCCCTCACCCTGCTAGAAAGGGCAGCTGTCGTCTATGGCGACTGCCCCTCCGTCGTCTATACCACCACCACCTACACGTGGTCCCAAACCCACCGCCGATGTCTCCAGCTGGCCTCCTCCCTCTCATCCCACCTAGGCATCAAACGCTCTCAAGTCGTCTCTATAATCTGCCCCAACACCCCCGCCTTGTACGAGGTCCACTTTGCTGTCCCCATGGCGGGAGCCATCCTCAACAACATCAACACCCGTCTCGATGCCCGCACCGTCTCCGTCATCCTCCGCCACAGTGAATCAAAACTCGTCTTTGTCGACCACCTCCTTGCCTCTCTGGTCCTGGAAGCCCTCTCCCTCTTCCCACCACAGCTCCCTCGTCCACAACTCGTCCTCATCACCGATCATCCGGACGACGAGTCAATCCCAGGCATCGATCATTTCATAGACACGTACGAGAATCTAGTGGGAAGGGGAGATCCGCTGTTTAAGTGGGTCCGGCCCGTTTCCGAATGGGACCCTATCGTCTTAAACTACACGTCCGGAACGACGTCCTCTCCGAAGGGGGTCGTGCATTGCCACAGGGCAATTTACATCGTGGCCTTGAGTTCCCTGGTGGACTGGTCCGTGCCTAAAAACTGCGTCTATCTGTGGACCCTACCGATGTTCCACGCAAACGGGTGGTGCTTCCCTTGGGCCATGGCCGCCGTCGGCGGCACCAACATTTGCGTCCGCAAATTCGACGGCCCAAAAATCTACGACTTAATCGAAGCCCACGGCGTCACCCACATGTGCGCCGCCCCCGTCGTCCTCAACATGCTCGTCAACACCCGGAGATCCGACCCACTCCGAAATCCAGTTAACATTCTCACCGGTGGGGCTCCGCCTCCTTCGAGCGTTTTGCTCCGGGCCGAGTCGATCGGGTTCGTGGTGGGTCACGGCTTCGGGATGACGGAGACTGCGGGGGTCGTGCTGTCGTGTGCATGGAAGCCGGAGTGGAACAAACTCCCGGCGATGGAGCAGGCGAGGCTGAAAGCGAGGCAGGGAGTGATGACGATCGGGATGACGGAGGCGGACGCCGTGAATCCGGATTCGGGTTTGGGAGTGAAGCGAGACGGGTCTGAAGTCGGGGAGATAGTTCTGAGAGGCGGGTGCGTGATGCTCGGGTATTTGAAGGACCCATCGGGAACTGCCAATTGTATGAAAGACGGCTGGTTTTATACCGGTGACGTGGGTGTAATGCATCCCGATGGCTATTTGGAAATCAAGGACAGATCCAAGGACATTATCATAAGCGGAGGTGAGAACATGAGCAGCGTGGAGGTCGAGTCAGTGCTGTATGCGAACCCAGCTGTCAACGAGGCGGCGGTCGTGGCGAGGCCCGACGAGTTTTGGGGGGAGACGCCGTGCGCGTTCGTGAGCTTGAAGAGTGACGTCACTCCAAAGCCGACGGAGAAGGAGATCATGGAGTTTTGCCGGAAGAAGCTGCCGCACTTCATGGTGCCCAAAACGGTGGTGTTTAAGGACGAGCTTCCCAAGACTTCCACCGGAAAGATTCAGAAGTTTTTGCTGAGGGAGATTGCCAGAGGGATTGCGCTGTCTTCCTTATCTTCTCCTCCGCCGCCGCCGGCGTCGCTGACGACGAATAGGATGTGCGTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

613

Amino Acids

67.05

Weight (kDa)

7.02

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 66 - 449 7.5e-75 AMP-binding enzyme
AMP-binding_C PF13193 499 - 576 1.3e-25 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 698
AatII GACGTC 2 cut(s) 728, 1611
AccB1I GGYRCC 2 cut(s) 890, 1675
AccI GTMKAC 1 cut(s) 492
AccII CGCG 1 cut(s) 1586
AccIII TCCGGA 3 cut(s) 583, 716, 1261
AclWI GGATC 3 cut(s) 647, 1005, 1443
AcoI YGGCCR 1 cut(s) 876
AcsI RAATTY 1 cut(s) 911
AcuI CTGAAG 1 cut(s) 1317
AcvI CACGTG 1 cut(s) 261
AcyI GRCGYC 6 cut(s) 725, 954, 1251, 1577, 1608, 1805
AfaI GTAC 2 cut(s) 374, 629
AfiI CCNNNNNNNGG 5 cut(s) 261, 400, 401, 1189, 1268
AflIII ACRYGT 4 cut(s) 258, 624, 711, 963
AgeI ACCGGT 2 cut(s) 1046, 1397
AgsI TTSAA 2 cut(s) 1348, 1600
AhdI GACNNNNNGTC 1 cut(s) 592
AjiI CACGTC 2 cut(s) 714, 1405
AjnI CCWGG 3 cut(s) 516, 601, 786
AluBI AGCT 9 cut(s) 17, 148, 209, 292, 547, 1526, 1596, 1660, 1705
AluI AGCT 9 cut(s) 17, 148, 209, 292, 547, 1526, 1596, 1660, 1705
Alw26I GTCTC 7 cut(s) 290, 346, 437, 454, 1124, 1281, 1568
AlwI GGATC 3 cut(s) 647, 1005, 1443
Ama87I CYCGRG 1 cut(s) 1337
Aor13HI TCCGGA 3 cut(s) 583, 716, 1261
AoxI GGCC 8 cut(s) 294, 674, 774, 870, 876, 919, 1083, 1553
ApeKI GCWGC 4 cut(s) 145, 206, 1488, 1660
ApoI RAATTY 1 cut(s) 911
AsiGI ACCGGT 2 cut(s) 1046, 1397
AspA2I CCTAGG 1 cut(s) 316
AspLEI GCGC 3 cut(s) 971, 1586, 1771
AsuC2I CCSGG 3 cut(s) 1006, 1082, 1184
AsuHPI GGTGA 6 cut(s) 181, 565, 949, 1036, 1412, 1487
AvaI CYCGRG 1 cut(s) 1337
AvaII GGWCC 8 cut(s) 264, 379, 514, 670, 691, 798, 828, 1351
AvrII CCTAGG 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 1680
BanI GGYRCC 2 cut(s) 890, 1675
BanII GRGCYC 1 cut(s) 1057
BbrPI CACGTG 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 1767
BbvCI CCTCAGC 1 cut(s) 1745
BbvI GCAGC 4 cut(s) 157, 218, 1500, 1647
BccI CCATC 4 cut(s) 416, 1183, 1363, 1418
BceAI ACGGC 7 cut(s) 866, 934, 967, 1129, 1238, 1399, 1564
BcgI CGANNNNNNTGC 2 cut(s) 1057, 1091
BciT130I CCWGG 3 cut(s) 518, 603, 788
BcnI CCSGG 3 cut(s) 1006, 1082, 1184
BcoDI GTCTC 7 cut(s) 290, 346, 437, 454, 1124, 1281, 1568
BfaI CTAG 4 cut(s) 134, 197, 317, 638
BlnI CCTAGG 1 cut(s) 316
Bme1390I CCNGG 6 cut(s) 518, 603, 788, 1006, 1082, 1184
Bme18I GGWCC 8 cut(s) 264, 379, 514, 670, 691, 798, 828, 1351
BmeRI GACNNNNNGTC 1 cut(s) 592
BmeT110I CYCGRG 1 cut(s) 1337
BmgBI CACGTC 2 cut(s) 714, 1405
BmrFI CCNGG 6 cut(s) 518, 603, 788, 1006, 1082, 1184
BmsI GCATC 5 cut(s) 330, 427, 615, 1323, 1426
BpiI GAAGAC 1 cut(s) 1767
BplI GAGNNNNNCTC 2 cut(s) 396, 428
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 1745
BpuEI CTTGAG 2 cut(s) 318, 799
BpuMI CCSGG 3 cut(s) 1006, 1082, 1184
Bsa29I ATCGAT 1 cut(s) 609
BsaAI YACGTR 2 cut(s) 261, 627
BsaBI GATNNNNATC 1 cut(s) 1229
BsaHI GRCGYC 6 cut(s) 725, 954, 1251, 1577, 1608, 1805
BsaJI CCNNGG 8 cut(s) 316, 396, 601, 786, 866, 873, 949, 1452
BsaWI WCCGGW 6 cut(s) 583, 716, 1046, 1261, 1397, 1721
Bsc4I CCNNNNNNNGG 5 cut(s) 261, 400, 401, 1189, 1268
Bse118I RCCGGY 3 cut(s) 1046, 1397, 1801
Bse1I ACTGG 2 cut(s) 800, 1031
Bse3DI GCAATG 1 cut(s) 748
Bse8I GATNNNNATC 1 cut(s) 1229
BseAI TCCGGA 3 cut(s) 583, 716, 1261
BseBI CCWGG 3 cut(s) 518, 603, 788
BseCI ATCGAT 1 cut(s) 609
BseDI CCNNGG 8 cut(s) 316, 396, 601, 786, 866, 873, 949, 1452
BseGI GGATG 8 cut(s) 308, 408, 456, 580, 1128, 1242, 1417, 1830
BseJI GATNNNNATC 1 cut(s) 1229
BseLI CCNNNNNNNGG 5 cut(s) 261, 400, 401, 1189, 1268
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 2 cut(s) 1307, 1736
BseNI ACTGG 2 cut(s) 800, 1031
BseRI GAGGAG 2 cut(s) 289, 1779
BseSI GKGCMC 1 cut(s) 1680
BseXI GCAGC 4 cut(s) 157, 218, 1500, 1647
BseYI CCCAGC 1 cut(s) 1522
Bsh1236I CGCG 1 cut(s) 1586
Bsh1285I CGRYCG 3 cut(s) 1095, 1233, 1545
BshFI GGCC 8 cut(s) 296, 676, 776, 872, 878, 921, 1085, 1555
BshNI GGYRCC 2 cut(s) 890, 1675
BshTI ACCGGT 2 cut(s) 1046, 1397
BshVI ATCGAT 1 cut(s) 609
BsiEI CGRYCG 3 cut(s) 1095, 1233, 1545
BsiHKCI CYCGRG 1 cut(s) 1337
BsiWI CGTACG 1 cut(s) 627
BslFI GGGAC 3 cut(s) 250, 377, 704
BslI CCNNNNNNNGG 5 cut(s) 261, 400, 401, 1189, 1268
BsmAI GTCTC 7 cut(s) 290, 346, 437, 454, 1124, 1281, 1568
BsmBI CGTCTC 5 cut(s) 346, 437, 454, 1281, 1568
BsmFI GGGAC 3 cut(s) 250, 377, 704
BsnI GGCC 8 cut(s) 296, 676, 776, 872, 878, 921, 1085, 1555
BsoBI CYCGRG 1 cut(s) 1337
Bsp1286I GDGCHC 2 cut(s) 1057, 1680
Bsp13I TCCGGA 3 cut(s) 583, 716, 1261
Bsp143I GATC 8 cut(s) 577, 610, 652, 1010, 1092, 1230, 1448, 1635
Bsp19I CCATGG 2 cut(s) 396, 873
BspANI GGCC 8 cut(s) 296, 676, 776, 872, 878, 921, 1085, 1555
BspCNI CTCAG 2 cut(s) 1308, 1737
BspDI ATCGAT 1 cut(s) 609
BspEI TCCGGA 3 cut(s) 583, 716, 1261
BspFNI CGCG 1 cut(s) 1586
BspPI GGATC 3 cut(s) 647, 1005, 1443
BspT107I GGYRCC 2 cut(s) 890, 1675
BsrDI GCAATG 1 cut(s) 748
BsrFI RCCGGY 3 cut(s) 1046, 1397, 1801
BsrI ACTGG 2 cut(s) 800, 1031
BssAI RCCGGY 3 cut(s) 1046, 1397, 1801
BssECI CCNNGG 8 cut(s) 316, 396, 601, 786, 866, 873, 949, 1452
BssMI GATC 8 cut(s) 577, 610, 652, 1010, 1092, 1230, 1448, 1635
BssNI GRCGYC 6 cut(s) 725, 954, 1251, 1577, 1608, 1805
BssT1I CCWWGG 5 cut(s) 316, 396, 866, 873, 1452
Bst2UI CCWGG 3 cut(s) 518, 603, 788
Bst4CI ACNGT 3 cut(s) 448, 470, 1687
Bst6I CTCTTC 2 cut(s) 539, 1595
BstACI GRCGYC 6 cut(s) 725, 954, 1251, 1577, 1608, 1805
BstBAI YACGTR 2 cut(s) 261, 627
BstC8I GCNNGC 4 cut(s) 294, 442, 1197, 1803
BstDEI CTNAG 2 cut(s) 1316, 1745
BstDSI CCRYGG 3 cut(s) 396, 873, 949
BstF5I GGATG 8 cut(s) 308, 408, 456, 580, 1128, 1242, 1417, 1830
BstFNI CGCG 1 cut(s) 1586
BstHHI GCGC 3 cut(s) 971, 1586, 1771
BstKTI GATC 8 cut(s) 580, 613, 655, 1013, 1095, 1233, 1451, 1638
BstMAI GTCTC 7 cut(s) 290, 346, 437, 454, 1124, 1281, 1568
BstMBI GATC 8 cut(s) 577, 610, 652, 1010, 1092, 1230, 1448, 1635
BstMCI CGRYCG 3 cut(s) 1095, 1233, 1545
BstMWI GCNNNNNNNGC 6 cut(s) 327, 878, 1201, 1523, 1547, 1807
BstNI CCWGG 3 cut(s) 518, 603, 788
BstNSI RCATGY 2 cut(s) 967, 994
BstSCI CCNGG 6 cut(s) 516, 601, 786, 1004, 1080, 1182
BstSLI GKGCMC 1 cut(s) 1680
BstUI CGCG 1 cut(s) 1586
BstV1I GCAGC 4 cut(s) 157, 218, 1500, 1647
BstV2I GAAGAC 1 cut(s) 1767
BstX2I RGATCY 3 cut(s) 652, 1010, 1448
BstXI CCANNNNNNTGG 1 cut(s) 1687
BstYI RGATCY 3 cut(s) 652, 1010, 1448
Bsu15I ATCGAT 1 cut(s) 609
BsuRI GGCC 8 cut(s) 296, 676, 776, 872, 878, 921, 1085, 1555
BsuTUI ATCGAT 1 cut(s) 609
BtgI CCRYGG 3 cut(s) 396, 873, 949
BtgZI GCGATG 1 cut(s) 1202
BtrI CACGTC 2 cut(s) 714, 1405
BtsCI GGATG 8 cut(s) 308, 408, 456, 580, 1128, 1242, 1417, 1830
BtsIMutI CAGTG 2 cut(s) 475, 1512
Cac8I GCNNGC 4 cut(s) 294, 442, 1197, 1803
CfoI GCGC 3 cut(s) 971, 1586, 1771
Cfr10I RCCGGY 3 cut(s) 1046, 1397, 1801
ClaI ATCGAT 1 cut(s) 609
CseI GACGC 6 cut(s) 805, 892, 943, 1259, 1585, 1794
Csp6I GTAC 2 cut(s) 373, 628
CspAI ACCGGT 2 cut(s) 1046, 1397
CviAII CATG 8 cut(s) 397, 874, 964, 991, 1158, 1483, 1639, 1672
CviQI GTAC 2 cut(s) 373, 628
DdeI CTNAG 2 cut(s) 1316, 1745
DpnI GATC 8 cut(s) 579, 612, 654, 1012, 1094, 1232, 1450, 1637
DpnII GATC 8 cut(s) 577, 610, 652, 1010, 1092, 1230, 1448, 1635
DrdI GACNNNNNNGTC 1 cut(s) 698
DriI GACNNNNNGTC 1 cut(s) 592
DseDI GACNNNNNNGTC 1 cut(s) 698
EaeI YGGCCR 1 cut(s) 876
Eam1104I CTCTTC 2 cut(s) 539, 1595
Eam1105I GACNNNNNGTC 1 cut(s) 592
EarI CTCTTC 2 cut(s) 539, 1595
EciI GGCGGA 4 cut(s) 452, 1047, 1262, 1782
Eco130I CCWWGG 5 cut(s) 316, 396, 866, 873, 1452
Eco24I GRGCYC 1 cut(s) 1057
Eco47I GGWCC 8 cut(s) 264, 379, 514, 670, 691, 798, 828, 1351
Eco57I CTGAAG 1 cut(s) 1317
Eco72I CACGTG 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 1337
EcoO109I RGGNCCY 2 cut(s) 691, 1351
EcoRII CCWGG 3 cut(s) 516, 601, 786
EcoT14I CCWWGG 5 cut(s) 316, 396, 866, 873, 1452
EcoT22I ATGCAT 1 cut(s) 1419
EcoT38I GRGCYC 1 cut(s) 1057
ErhI CCWWGG 5 cut(s) 316, 396, 866, 873, 1452
Esp3I CGTCTC 5 cut(s) 346, 437, 454, 1281, 1568
FaeI CATG 8 cut(s) 400, 877, 967, 994, 1161, 1486, 1642, 1675
FaqI GGGAC 3 cut(s) 250, 377, 704
FatI CATG 8 cut(s) 396, 873, 963, 990, 1157, 1482, 1638, 1671
FauI CCCGC 5 cut(s) 373, 394, 449, 1129, 1316
FblI GTMKAC 1 cut(s) 492
FokI GGATG 7 cut(s) 295, 395, 443, 567, 1135, 1249, 1404
FriOI GRGCYC 1 cut(s) 1057
FspBI CTAG 4 cut(s) 134, 197, 317, 638
GlaI GCGC 3 cut(s) 970, 1585, 1770
GsaI CCCAGC 1 cut(s) 1526
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 8 cut(s) 296, 676, 776, 872, 878, 921, 1085, 1555
HgaI GACGC 6 cut(s) 805, 892, 943, 1259, 1585, 1794
HhaI GCGC 3 cut(s) 971, 1586, 1771
Hin1I GRCGYC 6 cut(s) 725, 954, 1251, 1577, 1608, 1805
Hin1II CATG 8 cut(s) 400, 877, 967, 994, 1161, 1486, 1642, 1675
Hin6I GCGC 3 cut(s) 969, 1584, 1769
HinP1I GCGC 3 cut(s) 969, 1584, 1769
HincII GTYRAC 4 cut(s) 493, 1000, 1036, 1531
HindII GTYRAC 4 cut(s) 493, 1000, 1036, 1531
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 8 cut(s) 473, 593, 634, 1088, 1258, 1265, 1502, 1729
HpaI GTTAAC 1 cut(s) 1036
HphI GGTGA 6 cut(s) 181, 565, 949, 1036, 1412, 1487
Hpy166II GTNNAC 8 cut(s) 382, 493, 557, 793, 828, 1000, 1036, 1531
Hpy188I TCNGA 7 cut(s) 685, 735, 1015, 1025, 1297, 1317, 1734
Hpy188III TCNNGA 9 cut(s) 434, 584, 717, 1120, 1234, 1262, 1359, 1421, 1591
Hpy8I GTNNAC 8 cut(s) 382, 493, 557, 793, 828, 1000, 1036, 1531
HpyAV CCTTC 5 cut(s) 639, 730, 1074, 1342, 1624
HpyCH4III ACNGT 3 cut(s) 448, 470, 1687
HpyCH4IV ACGT 8 cut(s) 21, 126, 260, 626, 713, 725, 1404, 1608
HpyCH4V TGCA 5 cut(s) 4, 164, 748, 1157, 1417
HpyF10VI GCNNNNNNNGC 6 cut(s) 327, 878, 1201, 1523, 1547, 1807
HpyF3I CTNAG 2 cut(s) 1316, 1745
HpySE526I ACGT 8 cut(s) 21, 126, 260, 626, 713, 725, 1404, 1608
Hsp92I GRCGYC 6 cut(s) 725, 954, 1251, 1577, 1608, 1805
Hsp92II CATG 8 cut(s) 400, 877, 967, 994, 1161, 1486, 1642, 1675
HspAI GCGC 3 cut(s) 969, 1584, 1769
KflI GGGWCCC 1 cut(s) 691
Kpn2I TCCGGA 3 cut(s) 583, 716, 1261
KroI GCCGGC 1 cut(s) 1801
KroNI GCCGGC 1 cut(s) 1803
KspAI GTTAAC 1 cut(s) 1036
Kzo9I GATC 8 cut(s) 577, 610, 652, 1010, 1092, 1230, 1448, 1635
LmnI GCTCC 6 cut(s) 142, 404, 552, 1060, 1083, 1192
Lsp1109I GCAGC 4 cut(s) 157, 218, 1500, 1647
LweI GCATC 5 cut(s) 330, 427, 615, 1323, 1426
MaeI CTAG 4 cut(s) 134, 197, 317, 638
MaeII ACGT 8 cut(s) 21, 126, 260, 626, 713, 725, 1404, 1608
MaeIII GTNAC 5 cut(s) 955, 1109, 1400, 1604, 1609
MalI GATC 8 cut(s) 579, 612, 654, 1012, 1094, 1232, 1450, 1637
MboI GATC 8 cut(s) 577, 610, 652, 1010, 1092, 1230, 1448, 1635
MboII GAAGA 5 cut(s) 526, 1612, 1666, 1767, 1776
MfeI CAATTG 1 cut(s) 1369
MflI RGATCY 3 cut(s) 652, 1010, 1448
MhlI GDGCHC 2 cut(s) 1057, 1680
MluCI AATT 4 cut(s) 121, 762, 911, 1369
MlyI GAGTC 3 cut(s) 602, 1097, 1511
MmeI TCCRAC 1 cut(s) 1038
Mph1103I ATGCAT 1 cut(s) 1419
MreI CGCCGGCG 1 cut(s) 1801
MroI TCCGGA 3 cut(s) 583, 716, 1261
MroNI GCCGGC 1 cut(s) 1801
MseI TTAA 7 cut(s) 120, 129, 663, 704, 938, 1035, 1695
MslI CAYNNNNRTG 2 cut(s) 962, 1422
MspA1I CMGCKG 4 cut(s) 209, 292, 658, 1526
MspR9I CCNGG 6 cut(s) 518, 603, 788, 1006, 1082, 1184
MunI CAATTG 1 cut(s) 1369
MvaI CCWGG 3 cut(s) 518, 603, 788
MvnI CGCG 1 cut(s) 1586
MwoI GCNNNNNNNGC 6 cut(s) 327, 878, 1201, 1523, 1547, 1807
NaeI GCCGGC 1 cut(s) 1803
NciI CCSGG 3 cut(s) 1006, 1082, 1184
NcoI CCATGG 2 cut(s) 396, 873
NdeII GATC 8 cut(s) 577, 610, 652, 1010, 1092, 1230, 1448, 1635
NgoMIV GCCGGC 1 cut(s) 1801
NlaIII CATG 8 cut(s) 400, 877, 967, 994, 1161, 1486, 1642, 1675
NmeAIII GCCGAG 1 cut(s) 1111
NmuCI GTSAC 5 cut(s) 955, 1109, 1400, 1604, 1609
NsiI ATGCAT 1 cut(s) 1419
NspI RCATGY 2 cut(s) 967, 994
PciI ACATGT 1 cut(s) 963
PcsI WCGNNNNNNNCGW 7 cut(s) 219, 489, 719, 731, 939, 1583, 1814
PdiI GCCGGC 1 cut(s) 1803
PfeI GAWTC 5 cut(s) 473, 634, 1258, 1265, 1729
Pfl23II CGTACG 1 cut(s) 627
PfoI TCCNGGA 1 cut(s) 516
PinAI ACCGGT 2 cut(s) 1046, 1397
Ple19I CGATCG 2 cut(s) 1095, 1233
PleI GAGTC 3 cut(s) 601, 1096, 1510
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
PpsI GAGTC 3 cut(s) 601, 1096, 1510
Ppu21I YACGTR 2 cut(s) 261, 627
PpuMI RGGWCCY 2 cut(s) 691, 1351
PscI ACATGT 1 cut(s) 963
Psp5II RGGWCCY 2 cut(s) 691, 1351
Psp6I CCWGG 3 cut(s) 516, 601, 786
PspCI CACGTG 1 cut(s) 261
PspFI CCCAGC 1 cut(s) 1522
PspGI CCWGG 3 cut(s) 516, 601, 786
PspLI CGTACG 1 cut(s) 627
PspPPI RGGWCCY 2 cut(s) 691, 1351
PsuI RGATCY 3 cut(s) 652, 1010, 1448
PvuI CGATCG 2 cut(s) 1095, 1233
PvuII CAGCTG 3 cut(s) 209, 292, 1526
RsaI GTAC 2 cut(s) 374, 629
RsaNI GTAC 2 cut(s) 373, 628
RseI CAYNNNNRTG 2 cut(s) 962, 1422
SalI GTCGAC 1 cut(s) 491
SaqAI TTAA 7 cut(s) 120, 129, 663, 704, 938, 1035, 1695
Sau3AI GATC 8 cut(s) 577, 610, 652, 1010, 1092, 1230, 1448, 1635
SchI GAGTC 3 cut(s) 602, 1097, 1511
ScrFI CCNGG 6 cut(s) 518, 603, 788, 1006, 1082, 1184
SduI GDGCHC 2 cut(s) 1057, 1680
SfaNI GCATC 5 cut(s) 330, 427, 615, 1323, 1426
SgrAI CRCCGGYG 2 cut(s) 1046, 1801
SinI GGWCC 8 cut(s) 264, 379, 514, 670, 691, 798, 828, 1351
SmiMI CAYNNNNRTG 2 cut(s) 962, 1422
SmlI CTYRAG 2 cut(s) 333, 778
SmoI CTYRAG 2 cut(s) 333, 778
Sse9I AATT 4 cut(s) 121, 762, 911, 1369
SspMI CTAG 4 cut(s) 134, 197, 317, 638
StyD4I CCNGG 6 cut(s) 516, 601, 786, 1004, 1080, 1182
StyI CCWWGG 5 cut(s) 316, 396, 866, 873, 1452
TaaI ACNGT 3 cut(s) 448, 470, 1687
TaiI ACGT 8 cut(s) 24, 129, 263, 629, 716, 728, 1407, 1611
TaqI TCGA 8 cut(s) 435, 492, 609, 915, 942, 1067, 1091, 1500
TasI AATT 4 cut(s) 121, 762, 911, 1369
TauI GCSGC 7 cut(s) 881, 891, 974, 1541, 1666, 1798, 1801
TfiI GAWTC 5 cut(s) 473, 634, 1258, 1265, 1729
Tru1I TTAA 7 cut(s) 120, 129, 663, 704, 938, 1035, 1695
Tru9I TTAA 7 cut(s) 120, 129, 663, 704, 938, 1035, 1695
TscAI CASTG 2 cut(s) 475, 1512
TseFI GTSAC 5 cut(s) 955, 1109, 1400, 1604, 1609
TseI GCWGC 4 cut(s) 145, 206, 1488, 1660
Tsp45I GTSAC 5 cut(s) 955, 1109, 1400, 1604, 1609
TspDTI ATGAA 3 cut(s) 607, 1391, 1660
TspGWI ACGGA 6 cut(s) 223, 442, 790, 1142, 1256, 1640
TspRI CASTG 2 cut(s) 475, 1512
VpaK11BI GGWCC 8 cut(s) 264, 379, 514, 670, 691, 798, 828, 1351
XapI RAATTY 1 cut(s) 911
XceI RCATGY 2 cut(s) 967, 994
XmaJI CCTAGG 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 492
XspI CTAG 4 cut(s) 134, 197, 317, 638
ZraI GACGTC 2 cut(s) 726, 1609
Zsp2I ATGCAT 1 cut(s) 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.