Rh3DG314300

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000677
Physical Location & Seq
Reverse (-)
33805299 .. 33805868
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG314300.1

Sequence Viewer

Length: 570 bp
ATGACCGACGCGGATGTGGTGGATCCGGATTCGGGTTTGAGAGTGAAACGAAACGGGTCGGATATTGGAGAAATAGTCATACGAGGCGGGTGCGTCATGTTGGGCTACCTCAAAGACCCGGAAACGACGAGCAAAACCCTGAAACAGAACGGGTGGTTTTACACCGGCGACGTCGGGGTTATGCACCCGGACGGGTATCTGGAGATAAAGGATCGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTAAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACGCCGCGGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAACCTGACGTCAGGCCGAGGCCGACGGAGAAGGAGATAATAGAGTTCTGTAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTCAAGGACGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTTATGCTGAGAGAGATTGCGAAAAATATTGGGTCTACATCAACGACGAGGTCCAGTCGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

189

Amino Acids

20.82

Weight (kDa)

5.96

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 88 - 165 6.2e-25 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 174, 378, 485
AccB1I GGYRCC 1 cut(s) 442
AccI GTMKAC 2 cut(s) 485, 539
AccII CGCG 3 cut(s) 11, 293, 353
AccIII TCCGGA 1 cut(s) 25
AciI CCGC 6 cut(s) 11, 87, 237, 291, 293, 305
AclWI GGATC 4 cut(s) 17, 30, 210, 219
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 1 cut(s) 433
AcyI GRCGYC 4 cut(s) 171, 344, 375, 482
AfiI CCNNNNNNNGG 1 cut(s) 32
AflII CTTAAG 1 cut(s) 250
AgsI TTSAA 1 cut(s) 463
AjiI CACGTC 1 cut(s) 226
AluBI AGCT 2 cut(s) 427, 472
AluI AGCT 2 cut(s) 427, 472
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 4 cut(s) 17, 30, 210, 219
Aor13HI TCCGGA 1 cut(s) 25
AoxI GGCC 3 cut(s) 306, 380, 386
ApeKI GCWGC 3 cut(s) 255, 427, 472
ApoI RAATTY 1 cut(s) 433
AspLEI GCGC 1 cut(s) 353
AspS9I GGNCC 1 cut(s) 555
AsuC2I CCSGG 2 cut(s) 119, 188
AsuHPI GGTGA 1 cut(s) 307
AvaII GGWCC 1 cut(s) 555
BamHI GGATCC 1 cut(s) 22
BanI GGYRCC 1 cut(s) 442
BbsI GAAGAC 2 cut(s) 455, 485
BbvI GCAGC 3 cut(s) 267, 414, 459
BceAI ACGGC 2 cut(s) 293, 331
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 2 cut(s) 119, 188
BcoDI GTCTC 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 415
BfrI CTTAAG 1 cut(s) 250
BglI GCCNNNNNGGC 1 cut(s) 314
BisI GCNGC 5 cut(s) 256, 291, 306, 428, 473
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 5 cut(s) 257, 292, 307, 429, 474
Bme1390I CCNGG 2 cut(s) 119, 188
Bme18I GGWCC 1 cut(s) 555
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 555
BmiI GGNNCC 2 cut(s) 24, 444
BmrFI CCNGG 2 cut(s) 119, 188
BpiI GAAGAC 2 cut(s) 455, 485
BpmI CTGGAG 1 cut(s) 221
Bpu1102I GCTNAGC 1 cut(s) 318
BpuMI CCSGG 2 cut(s) 119, 188
Bsa29I ATCGAT 1 cut(s) 214
BsaHI GRCGYC 4 cut(s) 171, 344, 375, 482
BsaJI CCNNGG 4 cut(s) 219, 291, 309, 383
BsaWI WCCGGW 1 cut(s) 25
BsaXI ACNNNNNCTCC 6 cut(s) 60, 90, 254, 284, 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 1 cut(s) 558
BseAI TCCGGA 1 cut(s) 25
BseCI ATCGAT 1 cut(s) 214
BseDI CCNNGG 4 cut(s) 219, 291, 309, 383
BseGI GGATG 1 cut(s) 19
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 503
BseNI ACTGG 1 cut(s) 558
BseX3I CGGCCG 1 cut(s) 306
BseXI GCAGC 3 cut(s) 267, 414, 459
Bsh1236I CGCG 3 cut(s) 11, 293, 353
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 3 cut(s) 308, 382, 388
BshNI GGYRCC 1 cut(s) 442
BshVI ATCGAT 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 309
BsiSI CCGG 4 cut(s) 26, 119, 165, 188
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 335
BsmBI CGTCTC 1 cut(s) 335
BsnI GGCC 3 cut(s) 308, 382, 388
Bsp13I TCCGGA 1 cut(s) 25
Bsp143I GATC 4 cut(s) 22, 211, 215, 228
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 6 cut(s) 11, 87, 237, 291, 293, 305
BspANI GGCC 3 cut(s) 308, 382, 388
BspCNI CTCAG 1 cut(s) 504
BspDI ATCGAT 1 cut(s) 214
BspEI TCCGGA 1 cut(s) 25
BspFNI CGCG 3 cut(s) 11, 293, 353
BspLI GGNNCC 2 cut(s) 24, 444
BspPI GGATC 4 cut(s) 17, 30, 210, 219
BspT107I GGYRCC 1 cut(s) 442
BspTI CTTAAG 1 cut(s) 250
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 1 cut(s) 558
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 219, 291, 309, 383
BssMI GATC 4 cut(s) 22, 211, 215, 228
BssNI GRCGYC 4 cut(s) 171, 344, 375, 482
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 454
BstACI GRCGYC 4 cut(s) 171, 344, 375, 482
BstAFI CTTAAG 1 cut(s) 250
BstDEI CTNAG 2 cut(s) 318, 512
BstDSI CCRYGG 2 cut(s) 291, 309
BstF5I GGATG 1 cut(s) 19
BstFNI CGCG 3 cut(s) 11, 293, 353
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 4 cut(s) 25, 214, 218, 231
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 4 cut(s) 22, 211, 215, 228
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstSCI CCNGG 2 cut(s) 117, 186
BstSFI CTRYAG 1 cut(s) 415
BstUI CGCG 3 cut(s) 11, 293, 353
BstV1I GCAGC 3 cut(s) 267, 414, 459
BstV2I GAAGAC 2 cut(s) 455, 485
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
BstZI CGGCCG 1 cut(s) 306
Bsu15I ATCGAT 1 cut(s) 214
BsuRI GGCC 3 cut(s) 308, 382, 388
BsuTUI ATCGAT 1 cut(s) 214
BtgI CCRYGG 2 cut(s) 291, 309
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 1 cut(s) 19
CfoI GCGC 1 cut(s) 353
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 1 cut(s) 555
Cfr42I CCGCGG 1 cut(s) 294
ClaI ATCGAT 1 cut(s) 214
CseI GACGC 3 cut(s) 17, 82, 352
CviAII CATG 2 cut(s) 97, 439
CviJI RGCY 9 cut(s) 105, 308, 317, 322, 363, 382, 388, 427, 472
CviKI_1 RGCY 9 cut(s) 105, 308, 317, 322, 363, 382, 388, 427, 472
DdeI CTNAG 2 cut(s) 318, 512
DpnI GATC 4 cut(s) 24, 213, 217, 230
DpnII GATC 4 cut(s) 22, 211, 215, 228
EaeI YGGCCR 1 cut(s) 306
EagI CGGCCG 1 cut(s) 306
EclXI CGGCCG 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 555
Eco52I CGGCCG 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 2 cut(s) 100, 442
FaiI YATR 6 cut(s) 80, 98, 182, 233, 440, 509
FatI CATG 2 cut(s) 96, 438
FauI CCCGC 2 cut(s) 80, 230
FbaI TGATCA 1 cut(s) 228
FblI GTMKAC 2 cut(s) 485, 539
Fnu4HI GCNGC 5 cut(s) 256, 291, 306, 428, 473
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 5 cut(s) 256, 291, 306, 428, 473
GlaI GCGC 1 cut(s) 352
GluI GCNGC 5 cut(s) 256, 291, 306, 428, 473
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 3 cut(s) 308, 382, 388
HapII CCGG 4 cut(s) 26, 119, 165, 188
HgaI GACGC 3 cut(s) 17, 82, 352
HhaI GCGC 1 cut(s) 353
Hin1I GRCGYC 4 cut(s) 171, 344, 375, 482
Hin1II CATG 2 cut(s) 100, 442
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HinfI GANTC 3 cut(s) 29, 269, 496
HpaII CCGG 4 cut(s) 26, 119, 165, 188
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 3 cut(s) 298, 486, 540
Hpy188I TCNGA 2 cut(s) 61, 501
Hpy188III TCNNGA 2 cut(s) 26, 200
Hpy8I GTNNAC 3 cut(s) 298, 486, 540
Hpy99I CGWCG 8 cut(s) 11, 130, 173, 176, 394, 487, 490, 553
HpyAV CCTTC 1 cut(s) 391
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4IV ACGT 4 cut(s) 171, 225, 375, 482
HpyCH4V TGCA 1 cut(s) 184
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 2 cut(s) 318, 512
HpySE526I ACGT 4 cut(s) 171, 225, 375, 482
Hsp92I GRCGYC 4 cut(s) 171, 344, 375, 482
Hsp92II CATG 2 cut(s) 100, 442
HspAI GCGC 1 cut(s) 351
Kpn2I TCCGGA 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 228
KspI CCGCGG 1 cut(s) 294
Kzo9I GATC 4 cut(s) 22, 211, 215, 228
Lsp1109I GCAGC 3 cut(s) 267, 414, 459
MaeII ACGT 4 cut(s) 171, 225, 375, 482
MalI GATC 4 cut(s) 24, 213, 217, 230
MboI GATC 4 cut(s) 22, 211, 215, 228
MboII GAAGA 3 cut(s) 460, 490, 505
MflI RGATCY 1 cut(s) 22
MluCI AATT 1 cut(s) 433
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 7 cut(s) 77, 119, 256, 295, 378, 413, 546
MroI TCCGGA 1 cut(s) 25
MseI TTAA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 293
MspCI CTTAAG 1 cut(s) 250
MspI CCGG 4 cut(s) 26, 119, 165, 188
MspR9I CCNGG 2 cut(s) 119, 188
MvnI CGCG 3 cut(s) 11, 293, 353
MwoI GCNNNNNNNGC 1 cut(s) 314
NciI CCSGG 2 cut(s) 119, 188
NdeII GATC 4 cut(s) 22, 211, 215, 228
NlaIII CATG 2 cut(s) 100, 442
NlaIV GGNNCC 2 cut(s) 24, 444
NmeAIII GCCGAG 1 cut(s) 408
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 350
PfeI GAWTC 2 cut(s) 29, 496
PflFI GACNNNGTC 1 cut(s) 553
PkrI GCNGC 5 cut(s) 257, 292, 307, 429, 474
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 24, 444
PspPI GGNCC 1 cut(s) 555
PsuI RGATCY 1 cut(s) 22
PsyI GACNNNGTC 1 cut(s) 553
SacII CCGCGG 1 cut(s) 294
SalI GTCGAC 1 cut(s) 484
SaqAI TTAA 1 cut(s) 251
SatI GCNGC 5 cut(s) 256, 291, 306, 428, 473
Sau3AI GATC 4 cut(s) 22, 211, 215, 228
Sau96I GGNCC 1 cut(s) 555
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 2 cut(s) 119, 188
SfcI CTRYAG 1 cut(s) 415
Sfr303I CCGCGG 1 cut(s) 294
SgrAI CRCCGGYG 1 cut(s) 164
SgrBI CCGCGG 1 cut(s) 294
SgrDI CGTCGACG 1 cut(s) 484
SinI GGWCC 1 cut(s) 555
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
Sse9I AATT 1 cut(s) 433
SsiI CCGC 6 cut(s) 11, 87, 237, 291, 293, 305
SspI AATATT 1 cut(s) 532
StyD4I CCNGG 2 cut(s) 117, 186
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 454
TaiI ACGT 4 cut(s) 174, 228, 378, 485
TaqI TCGA 2 cut(s) 214, 485
TaqII GACCGA 1 cut(s) 20
TasI AATT 1 cut(s) 433
TauI GCSGC 2 cut(s) 293, 308
TfiI GAWTC 2 cut(s) 29, 496
Tru1I TTAA 1 cut(s) 251
Tru9I TTAA 1 cut(s) 251
TseI GCWGC 3 cut(s) 255, 427, 472
TspDTI ATGAA 1 cut(s) 427
TspGWI ACGGA 1 cut(s) 407
Tth111I GACNNNGTC 1 cut(s) 553
Vha464I CTTAAG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 555
XapI RAATTY 1 cut(s) 433
XmiI GTMKAC 2 cut(s) 485, 539
ZraI GACGTC 3 cut(s) 172, 376, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.