Rroxscaffold_6G00394540

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000677
Physical Location & Seq
Forward (+)
15301314 .. 15302629
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394540.1

Sequence Viewer

Length: 1071 bp
ATGGAAGAGCTGAAGCCAACCCCCGCCAACTCTTCCCCTCTCACCCCACTCGGCTTCCTAGACCGAGCCGCCACCGTCTACGGCGACTGCCCTTCTGTCATCTACGAAGACACCACCTACACGTGGAGCCAGACACACCGTCGATGCCTCCGCGTCGCTTCCTCCATCTCCTCCCTCGGCATCCGACGCGGCCACGTCGTCTCCGTGCTCTCCCCCAACATCCCCGCCATGTACGAGCTCCACTTCGCCGTCCCCATGTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACCCCCGCACCATCTCGCTCCTCCTCCGCCACAGCCAATCCAAGCTCCTCTTCGTCGACCACACCCTCCGCTCCCTCGAAATGGTGGACAGCGGCGATCCGGACTTCCAGTGGACCCTACCAGAAGACGAGTGGGACCCGATGACGCTGAACTACACTTCCGGCACTACTTCCTCCCCCAAGGGAGTGGTCCACGGCCACAGGGGGCTCTTCATCATCACCGTCAACTCTCTCCTTGACTGGGCAGTACCCAAACAGCCGGTCTACCTATGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATTGCCGCCGTCGGTGGGACCAACGTTTGCGTCCGCAAATTCGACGCCCATGTCATCTACGACTTGATCAGACGACATGGGGTCACTCACATGTGCGGTGCACCGGTGGTCCTCAACATGCTAACGAATGTACCGAACAACGAGCCTTTGAGGAACCCGGTCCAGATCATGACCTCCGGAGCTCCGCCGCCCGCGGCGGTTCTGTGTCGGACTGAGTTGCTGGGATTCGTGGTGAGCCATGGGTACGGGTTGACAGAGACGGCAGGGCCGGTGGTGTCATGCGCGTGGAAGCCCGGGTGGAATAAGTTGCCGGCGACGGAGAGGGCAAGGTTGAAGTCGAGGCAGGGAGTGAGGATGGTGGCCATGACCCACGTCGACGTGGTGGATCCGGATACGGGTGCGCCAGTTAAACGCGATGGTTCATCGCTGGGGAGGTGGTTTTCAGAGGCGGGTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

356

Amino Acids

39.12

Weight (kDa)

8.21

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 343 8.5e-67 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 263
AccB7I CCANNNNNTGG 1 cut(s) 594
AccBSI CCGCTC 1 cut(s) 366
AccI GTMKAC 4 cut(s) 78, 351, 558, 987
AccII CGCG 5 cut(s) 153, 189, 806, 896, 1026
AccIII TCCGGA 3 cut(s) 394, 788, 1000
AclI AACGTT 1 cut(s) 636
AclWI GGATC 3 cut(s) 386, 992, 1005
AcoI YGGCCR 3 cut(s) 190, 490, 972
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 1 cut(s) 32
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 2 cut(s) 264, 657
AfaI GTAC 5 cut(s) 233, 543, 608, 744, 857
AfiI CCNNNNNNNGG 7 cut(s) 123, 260, 376, 535, 594, 627, 1007
AflIII ACRYGT 2 cut(s) 120, 702
AgeI ACCGGT 1 cut(s) 715
AgsI TTSAA 1 cut(s) 946
AhdI GACNNNNNGTC 2 cut(s) 138, 692
AjiI CACGTC 3 cut(s) 196, 985, 991
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AloI GAACNNNNNNTCC 2 cut(s) 437, 469
AluBI AGCT 5 cut(s) 10, 238, 340, 600, 794
AluI AGCT 5 cut(s) 10, 238, 340, 600, 794
Alw21I GWGCWC 4 cut(s) 210, 240, 715, 796
Alw26I GTCTC 2 cut(s) 205, 863
Alw44I GTGCAC 1 cut(s) 711
AlwI GGATC 3 cut(s) 386, 992, 1005
Ama87I CYCGRG 1 cut(s) 905
Aor13HI TCCGGA 3 cut(s) 394, 788, 1000
AoxI GGCC 4 cut(s) 190, 490, 878, 972
ApaLI GTGCAC 1 cut(s) 711
ApoI RAATTY 1 cut(s) 650
ArsI GACNNNNNNTTYG 2 cut(s) 540, 572
AsiGI ACCGGT 1 cut(s) 715
AspLEI GCGC 3 cut(s) 266, 896, 1015
AspS9I GGNCC 7 cut(s) 408, 430, 484, 630, 721, 772, 878
AsuC2I CCSGG 3 cut(s) 770, 906, 907
AsuHPI GGTGA 3 cut(s) 34, 505, 856
AvaI CYCGRG 1 cut(s) 905
AvaII GGWCC 6 cut(s) 408, 430, 484, 630, 721, 772
BaeGI GKGCMC 1 cut(s) 715
BaeI ACNNNNGTAYC 2 cut(s) 726, 759
BalI TGGCCA 1 cut(s) 974
BamHI GGATCC 1 cut(s) 997
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 3 cut(s) 240, 504, 796
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 114, 426
Bbv12I GWGCWC 4 cut(s) 210, 240, 715, 796
BccI CCATC 5 cut(s) 173, 287, 314, 961, 1022
BceAI ACGGC 7 cut(s) 97, 233, 251, 505, 605, 607, 888
BciVI GTATCC 1 cut(s) 997
BclI TGATCA 1 cut(s) 678
BcnI CCSGG 3 cut(s) 770, 906, 907
BcoDI GTCTC 2 cut(s) 205, 863
BfaI CTAG 1 cut(s) 59
BfoI RGCGCY 1 cut(s) 267
BfuI GTATCC 1 cut(s) 997
BisI GCNGC 6 cut(s) 69, 190, 388, 618, 800, 807
BlsI GCNGC 6 cut(s) 70, 191, 389, 619, 801, 808
Bme1390I CCNGG 3 cut(s) 770, 906, 907
Bme18I GGWCC 6 cut(s) 408, 430, 484, 630, 721, 772
BmeRI GACNNNNNGTC 2 cut(s) 138, 692
BmeT110I CYCGRG 1 cut(s) 905
BmgBI CACGTC 3 cut(s) 196, 985, 991
BmgT120I GGNCC 7 cut(s) 408, 430, 484, 630, 721, 772, 878
BmiI GGNNCC 8 cut(s) 128, 265, 410, 431, 432, 631, 767, 999
BmrFI CCNGG 3 cut(s) 770, 906, 907
BmrI ACTGGG 1 cut(s) 544
BmsI GCATC 2 cut(s) 134, 189
BmuI ACTGGG 1 cut(s) 544
BoxI GACNNNNGTC 1 cut(s) 983
BpiI GAAGAC 2 cut(s) 114, 426
BpmI CTGGAG 1 cut(s) 616
BpuMI CCSGG 3 cut(s) 770, 906, 907
BsaAI YACGTR 1 cut(s) 123
BsaHI GRCGYC 2 cut(s) 264, 657
BsaJI CCNNGG 6 cut(s) 175, 474, 487, 804, 850, 905
BsaWI WCCGGW 4 cut(s) 394, 715, 788, 1000
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 260, 376, 535, 594, 627, 1007
Bse118I RCCGGY 4 cut(s) 553, 715, 880, 922
Bse1I ACTGG 3 cut(s) 403, 539, 1016
BseAI TCCGGA 3 cut(s) 394, 788, 1000
BseDI CCNNGG 6 cut(s) 175, 474, 487, 804, 850, 905
BseGI GGATG 3 cut(s) 180, 219, 972
BseLI CCNNNNNNNGG 7 cut(s) 123, 260, 376, 535, 594, 627, 1007
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 3 cut(s) 403, 539, 1016
BseRI GAGGAG 4 cut(s) 160, 305, 308, 332
BseSI GKGCMC 1 cut(s) 715
BseYI CCCAGC 2 cut(s) 832, 1039
Bsh1236I CGCG 5 cut(s) 153, 189, 806, 896, 1026
BshFI GGCC 4 cut(s) 192, 492, 880, 974
BshNI GGYRCC 1 cut(s) 263
BshTI ACCGGT 1 cut(s) 715
BsiHKAI GWGCWC 4 cut(s) 210, 240, 715, 796
BsiHKCI CYCGRG 1 cut(s) 905
BsiWI CGTACG 1 cut(s) 606
BslFI GGGAC 3 cut(s) 236, 443, 643
BslI CCNNNNNNNGG 7 cut(s) 123, 260, 376, 535, 594, 627, 1007
BsmAI GTCTC 2 cut(s) 205, 863
BsmBI CGTCTC 2 cut(s) 205, 863
BsmFI GGGAC 3 cut(s) 236, 443, 643
BsnI GGCC 4 cut(s) 192, 492, 880, 974
BsoBI CYCGRG 1 cut(s) 905
Bsp1286I GDGCHC 5 cut(s) 210, 240, 504, 715, 796
Bsp13I TCCGGA 3 cut(s) 394, 788, 1000
Bsp143I GATC 4 cut(s) 391, 678, 777, 997
Bsp19I CCATGG 1 cut(s) 850
BspANI GGCC 4 cut(s) 192, 492, 880, 974
BspCNI CTCAG 1 cut(s) 817
BspEI TCCGGA 3 cut(s) 394, 788, 1000
BspFNI CGCG 5 cut(s) 153, 189, 806, 896, 1026
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 8 cut(s) 128, 265, 410, 431, 432, 631, 767, 999
BspPI GGATC 3 cut(s) 386, 992, 1005
BspQI GCTCTTC 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 263
BsrBI CCGCTC 1 cut(s) 366
BsrFI RCCGGY 4 cut(s) 553, 715, 880, 922
BsrI ACTGG 3 cut(s) 403, 539, 1016
BssAI RCCGGY 4 cut(s) 553, 715, 880, 922
BssECI CCNNGG 6 cut(s) 175, 474, 487, 804, 850, 905
BssMI GATC 4 cut(s) 391, 678, 777, 997
BssNI GRCGYC 2 cut(s) 264, 657
BssT1I CCWWGG 2 cut(s) 474, 850
Bst4CI ACNGT 3 cut(s) 76, 140, 517
Bst6I CTCTTC 3 cut(s) 37, 350, 509
BstACI GRCGYC 2 cut(s) 264, 657
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 804, 924
BstDEI CTNAG 1 cut(s) 825
BstDSI CCRYGG 3 cut(s) 487, 804, 850
BstF5I GGATG 3 cut(s) 180, 219, 972
BstFNI CGCG 5 cut(s) 153, 189, 806, 896, 1026
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 3 cut(s) 266, 896, 1015
BstKTI GATC 4 cut(s) 394, 681, 780, 1000
BstMAI GTCTC 2 cut(s) 205, 863
BstMBI GATC 4 cut(s) 391, 678, 777, 997
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNSI RCATGY 2 cut(s) 706, 733
BstPAI GACNNNNGTC 1 cut(s) 983
BstSCI CCNGG 3 cut(s) 768, 904, 905
BstSLI GKGCMC 1 cut(s) 715
BstUI CGCG 5 cut(s) 153, 189, 806, 896, 1026
BstV2I GAAGAC 2 cut(s) 114, 426
BstX2I RGATCY 1 cut(s) 997
BstXI CCANNNNNNTGG 1 cut(s) 481
BstYI RGATCY 1 cut(s) 997
BsuI GTATCC 1 cut(s) 997
BsuRI GGCC 4 cut(s) 192, 492, 880, 974
BtgI CCRYGG 3 cut(s) 487, 804, 850
BtgZI GCGATG 2 cut(s) 1020, 1041
BtrI CACGTC 3 cut(s) 196, 985, 991
BtsCI GGATG 3 cut(s) 180, 219, 972
BtsIMutI CAGTG 1 cut(s) 410
Cac8I GCNNGC 2 cut(s) 804, 924
CciI TCATGA 1 cut(s) 780
CfoI GCGC 3 cut(s) 266, 896, 1015
Cfr10I RCCGGY 4 cut(s) 553, 715, 880, 922
Cfr13I GGNCC 7 cut(s) 408, 430, 484, 630, 721, 772, 878
Cfr42I CCGCGG 1 cut(s) 807
Cfr9I CCCGGG 1 cut(s) 905
CseI GACGC 6 cut(s) 142, 195, 448, 577, 631, 665
Csp6I GTAC 5 cut(s) 232, 542, 607, 743, 856
CspAI ACCGGT 1 cut(s) 715
CviQI GTAC 5 cut(s) 232, 542, 607, 743, 856
DdeI CTNAG 1 cut(s) 825
DinI GGCGCC 1 cut(s) 265
DpnI GATC 4 cut(s) 393, 680, 779, 999
DpnII GATC 4 cut(s) 391, 678, 777, 997
DrdI GACNNNNNNGTC 1 cut(s) 662
DriI GACNNNNNGTC 2 cut(s) 138, 692
DseDI GACNNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 3 cut(s) 190, 490, 972
Eam1104I CTCTTC 3 cut(s) 37, 350, 509
Eam1105I GACNNNNNGTC 2 cut(s) 138, 692
EarI CTCTTC 3 cut(s) 37, 350, 509
EciI GGCGGA 2 cut(s) 311, 786
Ecl136II GAGCTC 2 cut(s) 238, 794
Eco130I CCWWGG 2 cut(s) 474, 850
Eco24I GRGCYC 3 cut(s) 240, 504, 796
Eco47I GGWCC 6 cut(s) 408, 430, 484, 630, 721, 772
Eco53kI GAGCTC 2 cut(s) 238, 794
Eco57I CTGAAG 1 cut(s) 32
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 905
EcoICRI GAGCTC 2 cut(s) 238, 794
EcoO109I RGGNCCY 1 cut(s) 430
EcoT14I CCWWGG 2 cut(s) 474, 850
EcoT38I GRGCYC 3 cut(s) 240, 504, 796
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 474, 850
Esp3I CGTCTC 2 cut(s) 205, 863
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 3 cut(s) 236, 443, 643
FauI CCCGC 6 cut(s) 31, 232, 296, 308, 811, 1054
FbaI TGATCA 1 cut(s) 678
FblI GTMKAC 4 cut(s) 78, 351, 558, 987
Fnu4HI GCNGC 6 cut(s) 69, 190, 388, 618, 800, 807
FokI GGATG 3 cut(s) 167, 206, 979
FriOI GRGCYC 3 cut(s) 240, 504, 796
Fsp4HI GCNGC 6 cut(s) 69, 190, 388, 618, 800, 807
FspBI CTAG 1 cut(s) 59
GlaI GCGC 3 cut(s) 265, 895, 1014
GluI GCNGC 6 cut(s) 69, 190, 388, 618, 800, 807
GsaI CCCAGC 2 cut(s) 836, 1043
GsuI CTGGAG 1 cut(s) 616
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 4 cut(s) 192, 492, 880, 974
HgaI GACGC 6 cut(s) 142, 195, 448, 577, 631, 665
HhaI GCGC 3 cut(s) 266, 896, 1015
Hin1I GRCGYC 2 cut(s) 264, 657
Hin6I GCGC 3 cut(s) 264, 894, 1013
HinP1I GCGC 3 cut(s) 264, 894, 1013
HincII GTYRAC 4 cut(s) 352, 520, 864, 988
HindII GTYRAC 4 cut(s) 352, 520, 864, 988
HinfI GANTC 1 cut(s) 837
HphI GGTGA 3 cut(s) 34, 505, 856
Hpy188I TCNGA 5 cut(s) 185, 576, 683, 822, 1057
Hpy188III TCNNGA 5 cut(s) 395, 775, 781, 789, 1001
HpyAV CCTTC 1 cut(s) 102
HpyCH4III ACNGT 3 cut(s) 76, 140, 517
HpyCH4IV ACGT 5 cut(s) 122, 195, 636, 984, 990
HpyCH4V TGCA 1 cut(s) 713
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 1 cut(s) 825
HpySE526I ACGT 5 cut(s) 122, 195, 636, 984, 990
Hsp92I GRCGYC 2 cut(s) 264, 657
HspAI GCGC 3 cut(s) 264, 894, 1013
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 430
Kpn2I TCCGGA 3 cut(s) 394, 788, 1000
KroI GCCGGC 1 cut(s) 922
KroNI GCCGGC 1 cut(s) 924
Ksp22I TGATCA 1 cut(s) 678
KspI CCGCGG 1 cut(s) 807
Kzo9I GATC 4 cut(s) 391, 678, 777, 997
LguI GCTCTTC 1 cut(s) 509
LmnI GCTCC 8 cut(s) 126, 243, 318, 345, 371, 597, 791, 799
LweI GCATC 2 cut(s) 134, 189
MaeI CTAG 1 cut(s) 59
MaeII ACGT 5 cut(s) 122, 195, 636, 984, 990
MaeIII GTNAC 1 cut(s) 694
MalI GATC 4 cut(s) 393, 680, 779, 999
MbiI CCGCTC 1 cut(s) 366
MboI GATC 4 cut(s) 391, 678, 777, 997
MboII GAAGA 6 cut(s) 17, 24, 119, 337, 431, 496
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 5 cut(s) 210, 240, 504, 715, 796
MlsI TGGCCA 1 cut(s) 974
MluCI AATT 1 cut(s) 650
MluNI TGGCCA 1 cut(s) 974
Mly113I GGCGCC 1 cut(s) 264
MmeI TCCRAC 2 cut(s) 208, 800
Mox20I TGGCCA 1 cut(s) 974
MroI TCCGGA 3 cut(s) 394, 788, 1000
MroNI GCCGGC 1 cut(s) 922
MscI TGGCCA 1 cut(s) 974
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 2 cut(s) 701, 895
Msp20I TGGCCA 1 cut(s) 974
MspA1I CMGCKG 2 cut(s) 387, 806
MspR9I CCNGG 3 cut(s) 770, 906, 907
MvnI CGCG 5 cut(s) 153, 189, 806, 896, 1026
MwoI GCNNNNNNNGC 1 cut(s) 186
NaeI GCCGGC 1 cut(s) 924
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 3 cut(s) 770, 906, 907
NcoI CCATGG 1 cut(s) 850
NdeII GATC 4 cut(s) 391, 678, 777, 997
NgoMIV GCCGGC 1 cut(s) 922
NlaIV GGNNCC 8 cut(s) 128, 265, 410, 431, 432, 631, 767, 999
NmeAIII GCCGAG 2 cut(s) 30, 156
NmuCI GTSAC 1 cut(s) 694
NspI RCATGY 2 cut(s) 706, 733
PagI TCATGA 1 cut(s) 780
PciI ACATGT 1 cut(s) 702
PciSI GCTCTTC 1 cut(s) 509
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 743
PdiI GCCGGC 1 cut(s) 924
PfeI GAWTC 1 cut(s) 837
Pfl23II CGTACG 1 cut(s) 606
PflMI CCANNNNNTGG 1 cut(s) 594
PinAI ACCGGT 1 cut(s) 715
PkrI GCNGC 6 cut(s) 70, 191, 389, 619, 801, 808
PluTI GGCGCC 1 cut(s) 267
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
Ppu21I YACGTR 1 cut(s) 123
PpuMI RGGWCCY 1 cut(s) 430
PscI ACATGT 1 cut(s) 702
PshAI GACNNNNGTC 1 cut(s) 983
Psp124BI GAGCTC 2 cut(s) 240, 796
Psp1406I AACGTT 1 cut(s) 636
Psp5II RGGWCCY 1 cut(s) 430
PspCI CACGTG 1 cut(s) 123
PspFI CCCAGC 2 cut(s) 832, 1039
PspLI CGTACG 1 cut(s) 606
PspN4I GGNNCC 8 cut(s) 128, 265, 410, 431, 432, 631, 767, 999
PspPI GGNCC 7 cut(s) 408, 430, 484, 630, 721, 772, 878
PspPPI RGGWCCY 1 cut(s) 430
PsuI RGATCY 1 cut(s) 997
RsaI GTAC 5 cut(s) 233, 543, 608, 744, 857
RsaNI GTAC 5 cut(s) 232, 542, 607, 743, 856
RseI CAYNNNNRTG 2 cut(s) 701, 895
SacI GAGCTC 2 cut(s) 240, 796
SacII CCGCGG 1 cut(s) 807
SalI GTCGAC 2 cut(s) 350, 986
SapI GCTCTTC 1 cut(s) 509
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 6 cut(s) 69, 190, 388, 618, 800, 807
Sau3AI GATC 4 cut(s) 391, 678, 777, 997
Sau96I GGNCC 7 cut(s) 408, 430, 484, 630, 721, 772, 878
ScrFI CCNGG 3 cut(s) 770, 906, 907
SduI GDGCHC 5 cut(s) 210, 240, 504, 715, 796
SfaNI GCATC 2 cut(s) 134, 189
SfoI GGCGCC 1 cut(s) 265
Sfr303I CCGCGG 1 cut(s) 807
SgrAI CRCCGGYG 1 cut(s) 715
SgrBI CCGCGG 1 cut(s) 807
SgrDI CGTCGACG 1 cut(s) 986
SinI GGWCC 6 cut(s) 408, 430, 484, 630, 721, 772
SmaI CCCGGG 1 cut(s) 907
SmiMI CAYNNNNRTG 2 cut(s) 701, 895
Sse9I AATT 1 cut(s) 650
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 2 cut(s) 240, 796
StyD4I CCNGG 3 cut(s) 768, 904, 905
StyI CCWWGG 2 cut(s) 474, 850
TaaI ACNGT 3 cut(s) 76, 140, 517
TaiI ACGT 5 cut(s) 125, 198, 639, 987, 993
TaqI TCGA 7 cut(s) 142, 294, 351, 372, 654, 950, 987
TaqII GACCGA 1 cut(s) 78
TasI AATT 1 cut(s) 650
TauI GCSGC 6 cut(s) 71, 192, 390, 620, 802, 809
TfiI GAWTC 1 cut(s) 837
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 1 cut(s) 410
TseFI GTSAC 1 cut(s) 694
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 2 cut(s) 496, 1023
TspGWI ACGGA 2 cut(s) 193, 944
TspMI CCCGGG 1 cut(s) 905
TspRI CASTG 1 cut(s) 410
Van91I CCANNNNNTGG 1 cut(s) 594
VneI GTGCAC 1 cut(s) 711
VpaK11BI GGWCC 6 cut(s) 408, 430, 484, 630, 721, 772
XapI RAATTY 1 cut(s) 650
XceI RCATGY 2 cut(s) 706, 733
XcmI CCANNNNNNNNNTGG 1 cut(s) 423
XmaI CCCGGG 1 cut(s) 905
XmiI GTMKAC 4 cut(s) 78, 351, 558, 987
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.