RLG00000023001

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000677
Physical Location & Seq
Forward (+)
16489809 .. 16491749
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023001

Sequence Viewer

Length: 1665 bp
ATGGACCAGCTGAAACCTAGTCCTGCAAACCTCTGCCCTCAAACTCCCCTGGACTTCTTAGACCGAGCAGCGATCGTCTACGGCGACTGCATCTCCATCATCTACAACTCCACCACCACCTACACCTGGTCCCAAACCCACCGTCGATGTCTCCAGCTGGCTTCCTCCATCTCCACCCTCGTCAAGAAATCCCAGGTCGTCTCCGTGGTGTCCCCCAACACTCCCGCCATGTACGAACTCCAATTTGCTGTCCCCATGTCGGGAGCCGTCCTCAACAACATCAACACCCGCCTCGACGCCAACGCCGTCTCCGTGATCCTCCAACACAGCGAATCCAAGCTCGTCTTCGTCGACTTCCTCTCCCTCAACCTCGTCCTCCAAGCCCTCTCCTTGTTCCCCTCTCACATCCCCACCCCACTTCTCGTCCTCATCAACGATCATCATCAACCCTCCTCAACGTCCACCGTTGACTTCATAGCGGACACGTACGACAATATGGTGGCCAAAGGCGATCCCGGGTTCGTTTGGGTCCGGCCGGCGAGCGAGTGGGACCCAATGGTGCTGAACTACACTTCAGGGACGACGTCAGCTCCGAAAGGTGCAGTACACTGCCACAGAGCTATCTTCAACGTGAGTCTCGTTTCAATCATTGACTGGTCGGTTCCAAAAAATTGTGTGTACCTTTGGACTCTACCAATGTTCCATGCAAACGGGTGGTGCTTCCCGTGGGCCATGGCCGCTGTGGGTGCGACCAACATCTGCCTCCGCAAGTTCAACGGTCCTATAATCTACGATCTGATCCAGAAACACAGAGTGACTCACATGTGCGCCGCGCCTGTCGTTCTCAACATGCTGGTCAACCACCCGAGAACCGACCCGCTTCCGAACCCGGTTTGTATCTTAACAGGCGGAGCTCCGCCTCCTTCGAGCGTCCTGCTCCGGGCCGAGTCGCTCGGGTTTGTTGTAAGTCACGGGTACGGGTTGACTGAAACTGCCGGGGTGGTGGTGTCGTGTGCCTGGAAGCCGGAGTGGAATAAGTTTCCGGTGACGGAGAAGGCGAGGTTGAAGGCGAGGCAAGGAGTGAGGACGGTTGGAATGACCGACGCGGATGTGGTGGATCCGGATTCGGGTTTGAGCGTGAAACGAAACGGGTCGGATGTTGGAGAAATAGTTATGCGAGGCGGGTGCGTCATGTTGGGTTACCTCAAAGACCCGGAAACGACGAGCAAAACCCTGAGACAGAACGGGTGGTTTTACACCGGCGACGTCGGGGTTATGCACCCGGACGGGTATCTGGAGATAAAGGATCGATCAAAGGACGTGATCATAAGCGGGGGAGAGAACTTAAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACGCCGCGGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGCTGACGTCAGGCCGAGGCCGACGGAGAAGGAGATAATAGAGTTCTGTAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTCAAGGACGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTTATGCTGAGAGAGATTGCGAAAAATATTGGGTCTACATCAACGACGAGGTCCAGTCGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

555

Amino Acids

60.74

Weight (kDa)

7.9

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 22 - 402 5.8e-72 AMP-binding enzyme
AMP-binding_C PF13193 453 - 530 6.9e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 4 cut(s) 587, 1269, 1473, 1580
AccB1I GGYRCC 1 cut(s) 1537
AccI GTMKAC 4 cut(s) 78, 351, 1580, 1634
AccII CGCG 4 cut(s) 833, 1106, 1388, 1448
AccIII TCCGGA 1 cut(s) 1120
AclWI GGATC 6 cut(s) 310, 506, 793, 1112, 1125, 1314
AcoI YGGCCR 4 cut(s) 501, 533, 735, 1401
AcsI RAATTY 1 cut(s) 1528
AcuI CTGAAG 1 cut(s) 558
AcyI GRCGYC 6 cut(s) 297, 584, 1266, 1439, 1470, 1577
AdeI CACNNNGTG 1 cut(s) 814
AfaI GTAC 5 cut(s) 233, 488, 606, 680, 977
AfiI CCNNNNNNNGG 5 cut(s) 126, 259, 260, 940, 1127
AflII CTTAAG 1 cut(s) 1345
AflIII ACRYGT 2 cut(s) 483, 822
AgsI TTSAA 5 cut(s) 628, 645, 775, 1066, 1558
AjiI CACGTC 1 cut(s) 1321
AjnI CCWGG 4 cut(s) 48, 125, 192, 1016
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 9 cut(s) 10, 157, 340, 590, 620, 914, 1466, 1522, 1567
AluI AGCT 9 cut(s) 10, 157, 340, 590, 620, 914, 1466, 1522, 1567
Alw21I GWGCWC 1 cut(s) 916
Alw26I GTCTC 6 cut(s) 155, 205, 313, 641, 1231, 1430
AlwI GGATC 6 cut(s) 310, 506, 793, 1112, 1125, 1314
Ama87I CYCGRG 3 cut(s) 515, 865, 953
Aor13HI TCCGGA 1 cut(s) 1120
AoxI GGCC 8 cut(s) 501, 533, 729, 735, 942, 1401, 1475, 1481
ApeKI GCWGC 4 cut(s) 68, 1350, 1522, 1567
ApoI RAATTY 1 cut(s) 1528
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
AspLEI GCGC 3 cut(s) 830, 835, 1448
AspS9I GGNCC 8 cut(s) 4, 129, 529, 550, 729, 779, 942, 1650
AsuC2I CCSGG 7 cut(s) 516, 517, 890, 941, 997, 1214, 1283
AsuHPI GGTGA 2 cut(s) 1057, 1402
AvaI CYCGRG 3 cut(s) 515, 865, 953
AvaII GGWCC 6 cut(s) 4, 129, 529, 550, 779, 1650
BalI TGGCCA 1 cut(s) 503
BamHI GGATCC 1 cut(s) 1117
BanI GGYRCC 1 cut(s) 1537
BanII GRGCYC 1 cut(s) 916
BbsI GAAGAC 3 cut(s) 337, 1550, 1580
Bbv12I GWGCWC 1 cut(s) 916
BbvI GCAGC 4 cut(s) 80, 1362, 1509, 1554
BccI CCATC 2 cut(s) 104, 176
BceAI ACGGC 5 cut(s) 97, 251, 290, 1388, 1426
BcgI CGANNNNNNTGC 4 cut(s) 916, 950, 1167, 1201
BciT130I CCWGG 4 cut(s) 50, 127, 194, 1018
BclI TGATCA 1 cut(s) 1323
BcnI CCSGG 7 cut(s) 516, 517, 890, 941, 997, 1214, 1283
BcoDI GTCTC 6 cut(s) 155, 205, 313, 641, 1231, 1430
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 1510
BfrI CTTAAG 1 cut(s) 1345
BglI GCCNNNNNGGC 1 cut(s) 1409
BisI GCNGC 8 cut(s) 69, 738, 831, 1351, 1386, 1401, 1523, 1568
BlpI GCTNAGC 1 cut(s) 1413
BlsI GCNGC 8 cut(s) 70, 739, 832, 1352, 1387, 1402, 1524, 1569
Bme18I GGWCC 6 cut(s) 4, 129, 529, 550, 779, 1650
BmeT110I CYCGRG 3 cut(s) 515, 865, 953
BmgBI CACGTC 1 cut(s) 1321
BmgT120I GGNCC 8 cut(s) 4, 129, 529, 550, 729, 779, 942, 1650
BmiI GGNNCC 8 cut(s) 131, 265, 530, 551, 552, 663, 1119, 1539
BmsI GCATC 1 cut(s) 99
BpiI GAAGAC 3 cut(s) 337, 1550, 1580
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 2 cut(s) 137, 1316
Bpu1102I GCTNAGC 1 cut(s) 1413
BpuMI CCSGG 7 cut(s) 516, 517, 890, 941, 997, 1214, 1283
Bsa29I ATCGAT 1 cut(s) 1309
BsaAI YACGTR 1 cut(s) 486
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 6 cut(s) 297, 584, 1266, 1439, 1470, 1577
BsaWI WCCGGW 2 cut(s) 1042, 1120
Bsc4I CCNNNNNNNGG 5 cut(s) 126, 259, 260, 940, 1127
Bse118I RCCGGY 2 cut(s) 535, 1259
Bse1I ACTGG 2 cut(s) 659, 1653
Bse8I GATNNNNATC 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 1120
BseBI CCWGG 4 cut(s) 50, 127, 194, 1018
BseCI ATCGAT 1 cut(s) 1309
BseGI GGATG 3 cut(s) 405, 1114, 1162
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 5 cut(s) 126, 259, 260, 940, 1127
BseMII CTCAG 2 cut(s) 1226, 1598
BseNI ACTGG 2 cut(s) 659, 1653
BseRI GAGGAG 1 cut(s) 442
BseX3I CGGCCG 2 cut(s) 533, 1401
BseXI GCAGC 4 cut(s) 80, 1362, 1509, 1554
BsgI GTGCAG 1 cut(s) 621
Bsh1236I CGCG 4 cut(s) 833, 1106, 1388, 1448
Bsh1285I CGRYCG 3 cut(s) 75, 536, 1404
BshFI GGCC 8 cut(s) 503, 535, 731, 737, 944, 1403, 1477, 1483
BshNI GGYRCC 1 cut(s) 1537
BshVI ATCGAT 1 cut(s) 1309
BsiEI CGRYCG 3 cut(s) 75, 536, 1404
BsiHKAI GWGCWC 1 cut(s) 916
BsiHKCI CYCGRG 3 cut(s) 515, 865, 953
BsiWI CGTACG 1 cut(s) 486
BslFI GGGAC 5 cut(s) 115, 196, 236, 563, 592
BslI CCNNNNNNNGG 5 cut(s) 126, 259, 260, 940, 1127
BsmAI GTCTC 6 cut(s) 155, 205, 313, 641, 1231, 1430
BsmBI CGTCTC 3 cut(s) 205, 313, 1430
BsmFI GGGAC 5 cut(s) 115, 196, 236, 563, 592
BsnI GGCC 8 cut(s) 503, 535, 731, 737, 944, 1403, 1477, 1483
BsoBI CYCGRG 3 cut(s) 515, 865, 953
Bsp1286I GDGCHC 1 cut(s) 916
Bsp13I TCCGGA 1 cut(s) 1120
Bsp1720I GCTNAGC 1 cut(s) 1413
Bsp19I CCATGG 1 cut(s) 732
BspANI GGCC 8 cut(s) 503, 535, 731, 737, 944, 1403, 1477, 1483
BspCNI CTCAG 2 cut(s) 1227, 1599
BspDI ATCGAT 1 cut(s) 1309
BspEI TCCGGA 1 cut(s) 1120
BspFNI CGCG 4 cut(s) 833, 1106, 1388, 1448
BspLI GGNNCC 8 cut(s) 131, 265, 530, 551, 552, 663, 1119, 1539
BspPI GGATC 6 cut(s) 310, 506, 793, 1112, 1125, 1314
BspT107I GGYRCC 1 cut(s) 1537
BspTI CTTAAG 1 cut(s) 1345
BsrFI RCCGGY 2 cut(s) 535, 1259
BsrI ACTGG 2 cut(s) 659, 1653
BssAI RCCGGY 2 cut(s) 535, 1259
BssNI GRCGYC 6 cut(s) 297, 584, 1266, 1439, 1470, 1577
BssT1I CCWWGG 1 cut(s) 732
Bst2UI CCWGG 4 cut(s) 50, 127, 194, 1018
Bst4CI ACNGT 5 cut(s) 143, 466, 779, 1090, 1549
BstACI GRCGYC 6 cut(s) 297, 584, 1266, 1439, 1470, 1577
BstAFI CTTAAG 1 cut(s) 1345
BstBAI YACGTR 1 cut(s) 486
BstC8I GCNNGC 3 cut(s) 159, 537, 541
BstDEI CTNAG 4 cut(s) 58, 1235, 1413, 1607
BstDSI CCRYGG 5 cut(s) 204, 725, 732, 1386, 1404
BstEII GGTNACC 1 cut(s) 1199
BstF5I GGATG 3 cut(s) 405, 1114, 1162
BstFNI CGCG 4 cut(s) 833, 1106, 1388, 1448
BstHHI GCGC 3 cut(s) 830, 835, 1448
BstMAI GTCTC 6 cut(s) 155, 205, 313, 641, 1231, 1430
BstMCI CGRYCG 3 cut(s) 75, 536, 1404
BstMWI GCNNNNNNNGC 3 cut(s) 737, 746, 1409
BstNI CCWGG 4 cut(s) 50, 127, 194, 1018
BstNSI RCATGY 2 cut(s) 826, 853
BstPI GGTNACC 1 cut(s) 1199
BstSFI CTRYAG 1 cut(s) 1510
BstUI CGCG 4 cut(s) 833, 1106, 1388, 1448
BstV1I GCAGC 4 cut(s) 80, 1362, 1509, 1554
BstV2I GAAGAC 3 cut(s) 337, 1550, 1580
BstX2I RGATCY 1 cut(s) 1117
BstYI RGATCY 1 cut(s) 1117
BstZI CGGCCG 2 cut(s) 533, 1401
Bsu15I ATCGAT 1 cut(s) 1309
BsuRI GGCC 8 cut(s) 503, 535, 731, 737, 944, 1403, 1477, 1483
BsuTUI ATCGAT 1 cut(s) 1309
BtgI CCRYGG 5 cut(s) 204, 725, 732, 1386, 1404
BtrI CACGTC 1 cut(s) 1321
BtsCI GGATG 3 cut(s) 405, 1114, 1162
BtsI GCAGTG 1 cut(s) 607
BtsIMutI CAGTG 1 cut(s) 607
Cac8I GCNNGC 3 cut(s) 159, 537, 541
CfoI GCGC 3 cut(s) 830, 835, 1448
Cfr10I RCCGGY 2 cut(s) 535, 1259
Cfr13I GGNCC 8 cut(s) 4, 129, 529, 550, 729, 779, 942, 1650
Cfr42I CCGCGG 1 cut(s) 1389
Cfr9I CCCGGG 1 cut(s) 515
ClaI ATCGAT 1 cut(s) 1309
CseI GACGC 5 cut(s) 305, 919, 1112, 1177, 1447
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 5 cut(s) 232, 487, 605, 679, 976
CviAII CATG 8 cut(s) 229, 256, 704, 733, 823, 850, 1192, 1534
CviQI GTAC 5 cut(s) 232, 487, 605, 679, 976
DdeI CTNAG 4 cut(s) 58, 1235, 1413, 1607
DraIII CACNNNGTG 1 cut(s) 814
EaeI YGGCCR 4 cut(s) 501, 533, 735, 1401
EagI CGGCCG 2 cut(s) 533, 1401
EciI GGCGGA 2 cut(s) 906, 924
Ecl136II GAGCTC 1 cut(s) 914
EclXI CGGCCG 2 cut(s) 533, 1401
Eco130I CCWWGG 1 cut(s) 732
Eco24I GRGCYC 1 cut(s) 916
Eco47I GGWCC 6 cut(s) 4, 129, 529, 550, 779, 1650
Eco52I CGGCCG 2 cut(s) 533, 1401
Eco53kI GAGCTC 1 cut(s) 914
Eco57I CTGAAG 1 cut(s) 558
Eco88I CYCGRG 3 cut(s) 515, 865, 953
Eco91I GGTNACC 1 cut(s) 1199
EcoICRI GAGCTC 1 cut(s) 914
EcoO109I RGGNCCY 1 cut(s) 550
EcoO65I GGTNACC 1 cut(s) 1199
EcoRII CCWGG 4 cut(s) 48, 125, 192, 1016
EcoT14I CCWWGG 1 cut(s) 732
EcoT38I GRGCYC 1 cut(s) 916
ErhI CCWWGG 1 cut(s) 732
Esp3I CGTCTC 3 cut(s) 205, 313, 1430
FaeI CATG 8 cut(s) 232, 259, 707, 736, 826, 853, 1195, 1537
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 5 cut(s) 115, 196, 236, 563, 592
FatI CATG 8 cut(s) 228, 255, 703, 732, 822, 849, 1191, 1533
FauI CCCGC 5 cut(s) 232, 296, 885, 1175, 1325
FbaI TGATCA 1 cut(s) 1323
FblI GTMKAC 4 cut(s) 78, 351, 1580, 1634
Fnu4HI GCNGC 8 cut(s) 69, 738, 831, 1351, 1386, 1401, 1523, 1568
FokI GGATG 3 cut(s) 392, 1121, 1169
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 8 cut(s) 69, 738, 831, 1351, 1386, 1401, 1523, 1568
FspBI CTAG 1 cut(s) 18
GlaI GCGC 3 cut(s) 829, 834, 1447
GluI GCNGC 8 cut(s) 69, 738, 831, 1351, 1386, 1401, 1523, 1568
GsuI CTGGAG 2 cut(s) 137, 1316
HaeIII GGCC 8 cut(s) 503, 535, 731, 737, 944, 1403, 1477, 1483
HgaI GACGC 5 cut(s) 305, 919, 1112, 1177, 1447
HhaI GCGC 3 cut(s) 830, 835, 1448
Hin1I GRCGYC 6 cut(s) 297, 584, 1266, 1439, 1470, 1577
Hin1II CATG 8 cut(s) 232, 259, 707, 736, 826, 853, 1195, 1537
Hin6I GCGC 3 cut(s) 828, 833, 1446
HinP1I GCGC 3 cut(s) 828, 833, 1446
HincII GTYRAC 5 cut(s) 352, 469, 859, 984, 1581
HindII GTYRAC 5 cut(s) 352, 469, 859, 984, 1581
HinfI GANTC 8 cut(s) 332, 634, 688, 817, 947, 1124, 1364, 1591
HphI GGTGA 2 cut(s) 1057, 1402
Hpy188I TCNGA 5 cut(s) 594, 798, 885, 1156, 1596
Hpy188III TCNNGA 5 cut(s) 184, 261, 802, 1121, 1295
HpyAV CCTTC 4 cut(s) 933, 1048, 1060, 1486
HpyCH4III ACNGT 5 cut(s) 143, 466, 779, 1090, 1549
HpyCH4IV ACGT 8 cut(s) 458, 485, 584, 630, 1266, 1320, 1470, 1577
HpyCH4V TGCA 5 cut(s) 26, 90, 602, 707, 1279
HpyF10VI GCNNNNNNNGC 3 cut(s) 737, 746, 1409
HpyF3I CTNAG 4 cut(s) 58, 1235, 1413, 1607
HpySE526I ACGT 8 cut(s) 458, 485, 584, 630, 1266, 1320, 1470, 1577
Hsp92I GRCGYC 6 cut(s) 297, 584, 1266, 1439, 1470, 1577
Hsp92II CATG 8 cut(s) 232, 259, 707, 736, 826, 853, 1195, 1537
HspAI GCGC 3 cut(s) 828, 833, 1446
KflI GGGWCCC 1 cut(s) 550
Kpn2I TCCGGA 1 cut(s) 1120
KroI GCCGGC 1 cut(s) 535
KroNI GCCGGC 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 1323
KspI CCGCGG 1 cut(s) 1389
LmnI GCTCC 5 cut(s) 263, 595, 911, 919, 942
Lsp1109I GCAGC 4 cut(s) 80, 1362, 1509, 1554
LweI GCATC 1 cut(s) 99
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 18
MaeII ACGT 8 cut(s) 458, 485, 584, 630, 1266, 1320, 1470, 1577
MaeIII GTNAC 4 cut(s) 814, 968, 1045, 1199
MboII GAAGA 5 cut(s) 337, 616, 1555, 1585, 1600
MflI RGATCY 1 cut(s) 1117
MhlI GDGCHC 1 cut(s) 916
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 3 cut(s) 242, 670, 1528
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 5 cut(s) 643, 682, 811, 956, 1373
MmeI TCCRAC 4 cut(s) 346, 1072, 1134, 1141
Mox20I TGGCCA 1 cut(s) 503
MroI TCCGGA 1 cut(s) 1120
MroNI GCCGGC 1 cut(s) 535
MscI TGGCCA 1 cut(s) 503
MseI TTAA 2 cut(s) 902, 1346
Msp20I TGGCCA 1 cut(s) 503
MspA1I CMGCKG 4 cut(s) 10, 157, 740, 1388
MspCI CTTAAG 1 cut(s) 1345
MteI GCGCNGCGC 1 cut(s) 831
MvaI CCWGG 4 cut(s) 50, 127, 194, 1018
MvnI CGCG 4 cut(s) 833, 1106, 1388, 1448
MwoI GCNNNNNNNGC 3 cut(s) 737, 746, 1409
NaeI GCCGGC 1 cut(s) 537
NciI CCSGG 7 cut(s) 516, 517, 890, 941, 997, 1214, 1283
NcoI CCATGG 1 cut(s) 732
NgoMIV GCCGGC 1 cut(s) 535
NlaIII CATG 8 cut(s) 232, 259, 707, 736, 826, 853, 1195, 1537
NlaIV GGNNCC 8 cut(s) 131, 265, 530, 551, 552, 663, 1119, 1539
NmeAIII GCCGAG 2 cut(s) 970, 1503
NmuCI GTSAC 3 cut(s) 814, 968, 1045
NspI RCATGY 2 cut(s) 826, 853
PciI ACATGT 1 cut(s) 822
PcsI WCGNNNNNNNCGW 9 cut(s) 81, 303, 309, 348, 590, 636, 1055, 1401, 1445
PdiI GCCGGC 1 cut(s) 537
PfeI GAWTC 3 cut(s) 332, 1124, 1591
Pfl23II CGTACG 1 cut(s) 486
PflFI GACNNNGTC 2 cut(s) 583, 1648
PkrI GCNGC 8 cut(s) 70, 739, 832, 1352, 1387, 1402, 1524, 1569
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 5 cut(s) 642, 682, 811, 955, 1372
PpsI GAGTC 5 cut(s) 642, 682, 811, 955, 1372
Ppu21I YACGTR 1 cut(s) 486
PpuMI RGGWCCY 1 cut(s) 550
PscI ACATGT 1 cut(s) 822
Psp124BI GAGCTC 1 cut(s) 916
Psp5II RGGWCCY 1 cut(s) 550
Psp6I CCWGG 4 cut(s) 48, 125, 192, 1016
PspEI GGTNACC 1 cut(s) 1199
PspGI CCWGG 4 cut(s) 48, 125, 192, 1016
PspLI CGTACG 1 cut(s) 486
PspN4I GGNNCC 8 cut(s) 131, 265, 530, 551, 552, 663, 1119, 1539
PspPI GGNCC 8 cut(s) 4, 129, 529, 550, 729, 779, 942, 1650
PspPPI RGGWCCY 1 cut(s) 550
PsuI RGATCY 1 cut(s) 1117
PsyI GACNNNGTC 2 cut(s) 583, 1648
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 10, 157
RsaI GTAC 5 cut(s) 233, 488, 606, 680, 977
RsaNI GTAC 5 cut(s) 232, 487, 605, 679, 976
SacI GAGCTC 1 cut(s) 916
SacII CCGCGG 1 cut(s) 1389
SalI GTCGAC 2 cut(s) 350, 1579
SaqAI TTAA 2 cut(s) 902, 1346
SatI GCNGC 8 cut(s) 69, 738, 831, 1351, 1386, 1401, 1523, 1568
Sau96I GGNCC 8 cut(s) 4, 129, 529, 550, 729, 779, 942, 1650
SchI GAGTC 5 cut(s) 643, 682, 811, 956, 1373
SduI GDGCHC 1 cut(s) 916
SexAI ACCWGGT 1 cut(s) 125
SfaNI GCATC 1 cut(s) 99
SfcI CTRYAG 1 cut(s) 1510
Sfr303I CCGCGG 1 cut(s) 1389
SgrAI CRCCGGYG 1 cut(s) 1259
SgrBI CCGCGG 1 cut(s) 1389
SgrDI CGTCGACG 1 cut(s) 1579
SinI GGWCC 6 cut(s) 4, 129, 529, 550, 779, 1650
SmaI CCCGGG 1 cut(s) 517
SmlI CTYRAG 1 cut(s) 1345
SmoI CTYRAG 1 cut(s) 1345
Sse9I AATT 3 cut(s) 242, 670, 1528
SspI AATATT 1 cut(s) 1627
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 1 cut(s) 916
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 5 cut(s) 143, 466, 779, 1090, 1549
TaiI ACGT 8 cut(s) 461, 488, 587, 633, 1269, 1323, 1473, 1580
TaqI TCGA 6 cut(s) 145, 294, 351, 926, 1309, 1580
TaqII GACCGA 2 cut(s) 78, 1115
TasI AATT 3 cut(s) 242, 670, 1528
TatI WGTACW 1 cut(s) 604
TauI GCSGC 4 cut(s) 740, 833, 1388, 1403
TfiI GAWTC 3 cut(s) 332, 1124, 1591
Tru1I TTAA 2 cut(s) 902, 1346
Tru9I TTAA 2 cut(s) 902, 1346
TscAI CASTG 1 cut(s) 614
TseFI GTSAC 3 cut(s) 814, 968, 1045
TseI GCWGC 4 cut(s) 68, 1350, 1522, 1567
Tsp45I GTSAC 3 cut(s) 814, 968, 1045
TspDTI ATGAA 2 cut(s) 463, 1522
TspGWI ACGGA 4 cut(s) 193, 301, 1064, 1502
TspMI CCCGGG 1 cut(s) 515
TspRI CASTG 1 cut(s) 614
Tth111I GACNNNGTC 2 cut(s) 583, 1648
Vha464I CTTAAG 1 cut(s) 1345
VpaK11BI GGWCC 6 cut(s) 4, 129, 529, 550, 779, 1650
XapI RAATTY 1 cut(s) 1528
XceI RCATGY 2 cut(s) 826, 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XmaI CCCGGG 1 cut(s) 515
XmiI GTMKAC 4 cut(s) 78, 351, 1580, 1634
XspI CTAG 1 cut(s) 18
ZraI GACGTC 4 cut(s) 585, 1267, 1471, 1578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.