pycom17g27180

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000677
Physical Location & Seq
Forward (+)
25472546 .. 25474222
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g27180.1

Sequence Viewer

Length: 1677 bp
ATGGAAGAGCTGAAGCCCACGTCAGCAAACTCATCCCCTCTAACACCCTTGGGCTTTCTGGAAAGAGCAGCCACCGTCTACGGTGACTGCCTGTCCGTTATCTACGAAGACAAAATCTACACTTGGACGCAGACCTACCGTCGATGTCTCCGCGTGGCTTCCTCTATATCATCCCTTGGTATCAAGACAGGCAACGTGGTGTCCGTTCTCTCCCCAAACATCCCAGCCATGTACGAGCTCCACTTCGTCGTGCCCATGTCCGGCGCCATCCTCAACAACATCAACACCCGCCTGGACACCCGCACCGTCTCCGTGCTCCTGCGCCACAGCGAATCAAAACTCGTCTTCGTTGACTACGAATTGCGACCCCTAATCCTCGAAGCCATCTCTCTCTTCCCACCAAACACCCCCTCCCCTCGACTCGTCCTCATCGCCGATTCGGGAACGCCGTCACGCGCAGCACCACTCGATCACTTCCTGTGCACGTATGAGGACCTCGTGGACAGAGGCAACGAGGGGTTCGAGTGGGTCCCGCCGTCGAACGAGTGGAATCCAATGACGCTGAATTACACGTCGGGGACCACATCGTCTCCCAAGGGAGTCGTTCACAGCCACAGAGGGGTCTTCATCATCACCGTCGACTCCCTGATCGACTGGGGTGTACCCAAACAGCCGGTGTACCTGTGGACCCTACCGATGTTCCACGCTAACGGGTGGAGCTACCCCTACGGGATGGCAGCCGTCGGTGCAACCAACATCTGCGTCCGTAAGTTCGACGCCCAAATCATCTACGCCATGATCAAACGACACCGAGTTACCCACATGTGCGGTGCGCCCGTGGTGCTGAACATGCTCACAAACGTCCCGTCAGGAACCGAAAAGCTCGAGTACCCGGTTCACATCCTGACCGCTGGAGCTCCGCCGCCGGCGCAGGTTCTATTCCGGACCGAGTCCATGGGTTTCGTGGTAAGTCACGGGTATGGGCTGACCGAAACAGCGGGGCTGGTGGTGTCGTGCGCGTGGAAGAGGAAGTGGGATTTGTTGCCGGCGACTGAGAGAGCGAGGCTGAAGTCGAGGCAGGGAGTGAGGACGGTCGGGCTGACCCGAATGGACGTGGTAAACCCGGACTCCGGACTGAGCGTAAAACGCGACGGCCTGTCGCTCGGCGAAGTCGTTTTGAGAGGCGGGTGCGTGATGCTAGGGTATTTGAAAGACCAATCGGGAACGGCCAAGTGCATGAAAGACGGGTGGTTATACACGGGTGACGTGGGCGTGATGCATCCCGACGGCTATCTGGAAATTAAGGACAGATCCAAGGACGTTATTATAAGCGGGGGCGAGAATCTGAGCAGTGTGGAGGTGGAGTCGGTGCTGTATACGAACCCGGCGGTCAACGAGGCGGCGGTGGTGGCGAGGCCGGACGGGTCTTGGGGGGAGACGCCGTGCGCATTCGTGAGCTTGAAGAGTGAGGTGAGGCCGAAGCCGACGGAGAAGGAGATCGATGGAGTATTGCAGGAAGAAGCTGCCGAAATACATGGTGCCAAAAACGGTGGTGTTTACGGACGAGCTTCCCAAGACATCGACCGGGAAGATTCAGAAGTTTGTGCTGAGAGAGATGGCGAAGGGTTTGGGTTCGTCGAGGCCGAGTCGAATGTAATTTTTATATTATTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

559

Amino Acids

61.08

Weight (kDa)

5.64

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 20 - 403 5.2e-70 AMP-binding enzyme
AMP-binding_C PF13193 453 - 511 2.3e-12 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1328
AasI GACNNNNNNGTC 1 cut(s) 586
Acc16I TGCGCA 1 cut(s) 1447
Acc36I ACCTGC 1 cut(s) 922
AccB1I GGYRCC 2 cut(s) 263, 1538
AccI GTMKAC 3 cut(s) 78, 639, 1376
AccII CGCG 4 cut(s) 153, 456, 1019, 1149
AccIII TCCGGA 2 cut(s) 942, 1130
AclWI GGATC 1 cut(s) 1305
AcoI YGGCCR 1 cut(s) 1227
AcuI CTGAAG 2 cut(s) 32, 1088
AcyI GRCGYC 3 cut(s) 264, 777, 1439
AfaI GTAC 4 cut(s) 233, 663, 680, 890
AfiI CCNNNNNNNGG 3 cut(s) 260, 619, 1130
AflIII ACRYGT 2 cut(s) 570, 822
AgsI TTSAA 2 cut(s) 1210, 1462
AhdI GACNNNNNGTC 3 cut(s) 91, 138, 1156
AjiI CACGTC 4 cut(s) 21, 573, 1114, 1267
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 8 cut(s) 10, 238, 720, 883, 917, 1458, 1523, 1568
AluI AGCT 8 cut(s) 10, 238, 720, 883, 917, 1458, 1523, 1568
Alw21I GWGCWC 4 cut(s) 240, 318, 485, 919
Alw26I GTCTC 4 cut(s) 152, 313, 594, 1430
Alw44I GTGCAC 1 cut(s) 481
AlwI GGATC 1 cut(s) 1305
Ama87I CYCGRG 1 cut(s) 884
Aor13HI TCCGGA 2 cut(s) 942, 1130
AoxI GGCC 5 cut(s) 1153, 1227, 1415, 1475, 1641
ApaLI GTGCAC 1 cut(s) 481
ApeKI GCWGC 4 cut(s) 68, 458, 737, 1523
ArsI GACNNNNNNTTYG 2 cut(s) 851, 883
AspLEI GCGC 7 cut(s) 266, 324, 458, 835, 931, 1019, 1448
AspS9I GGNCC 5 cut(s) 493, 529, 579, 687, 945
AsuC2I CCSGG 4 cut(s) 893, 1124, 1385, 1586
AsuHPI GGTGA 4 cut(s) 95, 625, 1274, 1483
AvaI CYCGRG 1 cut(s) 884
AvaII GGWCC 5 cut(s) 493, 529, 579, 687, 945
BaeGI GKGCMC 2 cut(s) 255, 485
BanI GGYRCC 2 cut(s) 263, 1538
BanII GRGCYC 2 cut(s) 240, 919
BauI CACGAG 1 cut(s) 497
BbsI GAAGAC 3 cut(s) 114, 337, 616
Bbv12I GWGCWC 4 cut(s) 240, 318, 485, 919
BbvI GCAGC 4 cut(s) 80, 470, 749, 1510
BccI CCATC 5 cut(s) 275, 392, 727, 1496, 1610
BceAI ACGGC 7 cut(s) 433, 520, 725, 1168, 1242, 1303, 1426
BciT130I CCWGG 1 cut(s) 293
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 4 cut(s) 893, 1124, 1385, 1586
BcoDI GTCTC 4 cut(s) 152, 313, 594, 1430
BfaI CTAG 1 cut(s) 1199
BfoI RGCGCY 1 cut(s) 267
BfuAI ACCTGC 1 cut(s) 922
BisI GCNGC 6 cut(s) 69, 459, 738, 923, 1401, 1524
BlsI GCNGC 6 cut(s) 70, 460, 739, 924, 1402, 1525
Bme1390I CCNGG 5 cut(s) 293, 893, 1124, 1385, 1586
Bme18I GGWCC 5 cut(s) 493, 529, 579, 687, 945
BmeRI GACNNNNNGTC 3 cut(s) 91, 138, 1156
BmeT110I CYCGRG 1 cut(s) 884
BmgBI CACGTC 4 cut(s) 21, 573, 1114, 1267
BmgT120I GGNCC 5 cut(s) 493, 529, 579, 687, 945
BmiI GGNNCC 7 cut(s) 265, 530, 531, 580, 689, 874, 1540
BmrFI CCNGG 5 cut(s) 293, 893, 1124, 1385, 1586
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 3 cut(s) 1185, 1266, 1288
BmuI ACTGGG 1 cut(s) 664
BpiI GAAGAC 3 cut(s) 114, 337, 616
BpmI CTGGAG 1 cut(s) 933
BpuMI CCSGG 4 cut(s) 893, 1124, 1385, 1586
Bsa29I ATCGAT 1 cut(s) 1500
BsaAI YACGTR 1 cut(s) 486
BsaHI GRCGYC 3 cut(s) 264, 777, 1439
BsaJI CCNNGG 6 cut(s) 48, 175, 594, 837, 954, 1314
BsaWI WCCGGW 2 cut(s) 942, 1130
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 619, 1130
Bse118I RCCGGY 3 cut(s) 673, 925, 1045
Bse1I ACTGG 1 cut(s) 659
BseAI TCCGGA 2 cut(s) 942, 1130
BseBI CCWGG 1 cut(s) 293
BseCI ATCGAT 1 cut(s) 1500
BseDI CCNNGG 6 cut(s) 48, 175, 594, 837, 954, 1314
BseGI GGATG 7 cut(s) 32, 170, 219, 267, 738, 900, 1279
BseLI CCNNNNNNNGG 3 cut(s) 260, 619, 1130
BseMII CTCAG 4 cut(s) 1044, 1127, 1337, 1599
BseNI ACTGG 1 cut(s) 659
BseSI GKGCMC 2 cut(s) 255, 485
BseXI GCAGC 4 cut(s) 80, 470, 749, 1510
BseYI CCCAGC 1 cut(s) 223
Bsh1236I CGCG 4 cut(s) 153, 456, 1019, 1149
Bsh1285I CGRYCG 2 cut(s) 1095, 1585
BshFI GGCC 5 cut(s) 1155, 1229, 1417, 1477, 1643
BshNI GGYRCC 2 cut(s) 263, 1538
BshVI ATCGAT 1 cut(s) 1500
BsiEI CGRYCG 2 cut(s) 1095, 1585
BsiHKAI GWGCWC 4 cut(s) 240, 318, 485, 919
BsiHKCI CYCGRG 1 cut(s) 884
BslFI GGGAC 3 cut(s) 515, 592, 848
BslI CCNNNNNNNGG 3 cut(s) 260, 619, 1130
BsmAI GTCTC 4 cut(s) 152, 313, 594, 1430
BsmBI CGTCTC 3 cut(s) 313, 594, 1430
BsmFI GGGAC 3 cut(s) 515, 592, 848
BsmI GAATGC 1 cut(s) 1448
BsnI GGCC 5 cut(s) 1155, 1229, 1417, 1477, 1643
BsoBI CYCGRG 1 cut(s) 884
Bsp1286I GDGCHC 5 cut(s) 240, 255, 318, 485, 919
Bsp13I TCCGGA 2 cut(s) 942, 1130
Bsp143I GATC 5 cut(s) 469, 648, 798, 1310, 1497
Bsp19I CCATGG 1 cut(s) 954
BspANI GGCC 5 cut(s) 1155, 1229, 1417, 1477, 1643
BspCNI CTCAG 4 cut(s) 1045, 1128, 1338, 1600
BspDI ATCGAT 1 cut(s) 1500
BspEI TCCGGA 2 cut(s) 942, 1130
BspFNI CGCG 4 cut(s) 153, 456, 1019, 1149
BspLI GGNNCC 7 cut(s) 265, 530, 531, 580, 689, 874, 1540
BspMI ACCTGC 1 cut(s) 922
BspPI GGATC 1 cut(s) 1305
BspT107I GGYRCC 2 cut(s) 263, 1538
BsrFI RCCGGY 3 cut(s) 673, 925, 1045
BsrI ACTGG 1 cut(s) 659
BssAI RCCGGY 3 cut(s) 673, 925, 1045
BssECI CCNNGG 6 cut(s) 48, 175, 594, 837, 954, 1314
BssMI GATC 5 cut(s) 469, 648, 798, 1310, 1497
BssNAI GTATAC 1 cut(s) 1377
BssNI GRCGYC 3 cut(s) 264, 777, 1439
BssSI CACGAG 1 cut(s) 497
BssT1I CCWWGG 5 cut(s) 48, 175, 594, 954, 1314
Bst1107I GTATAC 1 cut(s) 1377
Bst2BI CACGAG 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 7 cut(s) 76, 83, 140, 307, 637, 1093, 1550
Bst6I CTCTTC 3 cut(s) 398, 1019, 1457
BstACI GRCGYC 3 cut(s) 264, 777, 1439
BstBAI YACGTR 1 cut(s) 486
BstC8I GCNNGC 2 cut(s) 927, 1047
BstDEI CTNAG 4 cut(s) 1053, 1136, 1346, 1608
BstDSI CCRYGG 2 cut(s) 837, 954
BstF5I GGATG 7 cut(s) 32, 170, 219, 267, 738, 900, 1279
BstFNI CGCG 4 cut(s) 153, 456, 1019, 1149
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 7 cut(s) 266, 324, 458, 835, 931, 1019, 1448
BstKTI GATC 5 cut(s) 472, 651, 801, 1313, 1500
BstMAI GTCTC 4 cut(s) 152, 313, 594, 1430
BstMBI GATC 5 cut(s) 469, 648, 798, 1310, 1497
BstMCI CGRYCG 2 cut(s) 1095, 1585
BstMWI GCNNNNNNNGC 6 cut(s) 746, 841, 850, 928, 1146, 1409
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 2 cut(s) 826, 853
BstSCI CCNGG 5 cut(s) 291, 891, 1122, 1383, 1584
BstSLI GKGCMC 2 cut(s) 255, 485
BstUI CGCG 4 cut(s) 153, 456, 1019, 1149
BstV1I GCAGC 4 cut(s) 80, 470, 749, 1510
BstV2I GAAGAC 3 cut(s) 114, 337, 616
BstX2I RGATCY 1 cut(s) 1310
BstYI RGATCY 1 cut(s) 1310
BstZ17I GTATAC 1 cut(s) 1377
Bsu15I ATCGAT 1 cut(s) 1500
BsuRI GGCC 5 cut(s) 1155, 1229, 1417, 1477, 1643
BsuTUI ATCGAT 1 cut(s) 1500
BtgI CCRYGG 2 cut(s) 837, 954
BtgZI GCGATG 1 cut(s) 415
BtrI CACGTC 4 cut(s) 21, 573, 1114, 1267
BtsCI GGATG 7 cut(s) 32, 170, 219, 267, 738, 900, 1279
BtsI GCAGTG 1 cut(s) 1357
BtsIMutI CAGTG 1 cut(s) 1357
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 2 cut(s) 927, 1047
CfoI GCGC 7 cut(s) 266, 324, 458, 835, 931, 1019, 1448
Cfr10I RCCGGY 3 cut(s) 673, 925, 1045
Cfr13I GGNCC 5 cut(s) 493, 529, 579, 687, 945
ClaI ATCGAT 1 cut(s) 1500
CpoI CGGWCCG 1 cut(s) 945
CseI GACGC 5 cut(s) 136, 568, 751, 785, 1447
Csp6I GTAC 4 cut(s) 232, 662, 679, 889
CspCI CAANNNNNGTGG 2 cut(s) 1531, 1566
CspI CGGWCCG 1 cut(s) 945
CviAII CATG 8 cut(s) 229, 256, 796, 823, 850, 955, 1237, 1535
CviQI GTAC 4 cut(s) 232, 662, 679, 889
DdeI CTNAG 4 cut(s) 1053, 1136, 1346, 1608
DinI GGCGCC 1 cut(s) 265
DpnI GATC 5 cut(s) 471, 650, 800, 1312, 1499
DpnII GATC 5 cut(s) 469, 648, 798, 1310, 1497
DrdI GACNNNNNNGTC 1 cut(s) 586
DriI GACNNNNNGTC 3 cut(s) 91, 138, 1156
DseDI GACNNNNNNGTC 1 cut(s) 586
EaeI YGGCCR 1 cut(s) 1227
Eam1104I CTCTTC 3 cut(s) 398, 1019, 1457
Eam1105I GACNNNNNGTC 3 cut(s) 91, 138, 1156
EarI CTCTTC 3 cut(s) 398, 1019, 1457
EciI GGCGGA 1 cut(s) 909
Ecl136II GAGCTC 2 cut(s) 238, 917
Eco130I CCWWGG 5 cut(s) 48, 175, 594, 954, 1314
Eco24I GRGCYC 2 cut(s) 240, 919
Eco47I GGWCC 5 cut(s) 493, 529, 579, 687, 945
Eco53kI GAGCTC 2 cut(s) 238, 917
Eco57I CTGAAG 2 cut(s) 32, 1088
Eco88I CYCGRG 1 cut(s) 884
EcoICRI GAGCTC 2 cut(s) 238, 917
EcoO109I RGGNCCY 2 cut(s) 493, 529
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 5 cut(s) 48, 175, 594, 954, 1314
EcoT22I ATGCAT 1 cut(s) 1281
EcoT38I GRGCYC 2 cut(s) 240, 919
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 5 cut(s) 48, 175, 594, 954, 1314
Esp3I CGTCTC 3 cut(s) 313, 594, 1430
FaeI CATG 8 cut(s) 232, 259, 799, 826, 853, 958, 1240, 1538
FaqI GGGAC 3 cut(s) 515, 592, 848
FatI CATG 8 cut(s) 228, 255, 795, 822, 849, 954, 1236, 1534
FauI CCCGC 6 cut(s) 296, 308, 540, 991, 1178, 1325
FbaI TGATCA 1 cut(s) 798
FblI GTMKAC 3 cut(s) 78, 639, 1376
Fnu4HI GCNGC 6 cut(s) 69, 459, 738, 923, 1401, 1524
FokI GGATG 7 cut(s) 19, 157, 206, 254, 745, 887, 1266
FriOI GRGCYC 2 cut(s) 240, 919
Fsp4HI GCNGC 6 cut(s) 69, 459, 738, 923, 1401, 1524
FspAI RTGCGCAY 1 cut(s) 1447
FspBI CTAG 1 cut(s) 1199
FspI TGCGCA 1 cut(s) 1447
GlaI GCGC 7 cut(s) 265, 323, 457, 834, 930, 1018, 1447
GluI GCNGC 6 cut(s) 69, 459, 738, 923, 1401, 1524
GsaI CCCAGC 1 cut(s) 227
GsuI CTGGAG 1 cut(s) 933
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 5 cut(s) 1155, 1229, 1417, 1477, 1643
HgaI GACGC 5 cut(s) 136, 568, 751, 785, 1447
HhaI GCGC 7 cut(s) 266, 324, 458, 835, 931, 1019, 1448
Hin1I GRCGYC 3 cut(s) 264, 777, 1439
Hin1II CATG 8 cut(s) 232, 259, 799, 826, 853, 958, 1240, 1538
Hin6I GCGC 7 cut(s) 264, 322, 456, 833, 929, 1017, 1446
HinP1I GCGC 7 cut(s) 264, 322, 456, 833, 929, 1017, 1446
HincII GTYRAC 3 cut(s) 352, 640, 1393
HindII GTYRAC 3 cut(s) 352, 640, 1393
HphI GGTGA 4 cut(s) 95, 625, 1274, 1483
Hpy188I TCNGA 2 cut(s) 1347, 1597
HpyAV CCTTC 2 cut(s) 1486, 1616
HpyCH4III ACNGT 7 cut(s) 76, 83, 140, 307, 637, 1093, 1550
HpyCH4IV ACGT 8 cut(s) 20, 195, 485, 572, 861, 1113, 1266, 1320
HpyCH4V TGCA 5 cut(s) 483, 749, 1236, 1279, 1513
HpyF10VI GCNNNNNNNGC 6 cut(s) 746, 841, 850, 928, 1146, 1409
HpyF3I CTNAG 4 cut(s) 1053, 1136, 1346, 1608
HpySE526I ACGT 8 cut(s) 20, 195, 485, 572, 861, 1113, 1266, 1320
Hsp92I GRCGYC 3 cut(s) 264, 777, 1439
Hsp92II CATG 8 cut(s) 232, 259, 799, 826, 853, 958, 1240, 1538
HspAI GCGC 7 cut(s) 264, 322, 456, 833, 929, 1017, 1446
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 529
Kpn2I TCCGGA 2 cut(s) 942, 1130
KroI GCCGGC 2 cut(s) 925, 1045
KroNI GCCGGC 2 cut(s) 927, 1047
Ksp22I TGATCA 1 cut(s) 798
Kzo9I GATC 5 cut(s) 469, 648, 798, 1310, 1497
LmnI GCTCC 5 cut(s) 243, 321, 717, 914, 922
Lsp1109I GCAGC 4 cut(s) 80, 470, 749, 1510
LweI GCATC 3 cut(s) 1185, 1266, 1288
MaeI CTAG 1 cut(s) 1199
MaeII ACGT 8 cut(s) 20, 195, 485, 572, 861, 1113, 1266, 1320
MaeIII GTNAC 5 cut(s) 83, 450, 814, 971, 1262
MalI GATC 5 cut(s) 471, 650, 800, 1312, 1499
MboI GATC 5 cut(s) 469, 648, 798, 1310, 1497
MboII GAAGA 9 cut(s) 17, 119, 337, 385, 616, 1036, 1474, 1529, 1601
MflI RGATCY 1 cut(s) 1310
MhlI GDGCHC 5 cut(s) 240, 255, 318, 485, 919
MluCI AATT 4 cut(s) 359, 565, 1299, 1656
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 7 cut(s) 414, 609, 635, 959, 1121, 1373, 1655
Mph1103I ATGCAT 1 cut(s) 1281
MreI CGCCGGCG 1 cut(s) 925
MroI TCCGGA 2 cut(s) 942, 1130
MroNI GCCGGC 2 cut(s) 925, 1045
MseI TTAA 2 cut(s) 1302, 1675
MslI CAYNNNNRTG 1 cut(s) 978
MspA1I CMGCKG 2 cut(s) 911, 998
MspR9I CCNGG 5 cut(s) 293, 893, 1124, 1385, 1586
Mva1269I GAATGC 1 cut(s) 1448
MvaI CCWGG 1 cut(s) 293
MvnI CGCG 4 cut(s) 153, 456, 1019, 1149
MwoI GCNNNNNNNGC 6 cut(s) 746, 841, 850, 928, 1146, 1409
NaeI GCCGGC 2 cut(s) 927, 1047
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 4 cut(s) 893, 1124, 1385, 1586
NcoI CCATGG 1 cut(s) 954
NdeII GATC 5 cut(s) 469, 648, 798, 1310, 1497
NgoMIV GCCGGC 2 cut(s) 925, 1045
NlaIII CATG 8 cut(s) 232, 259, 799, 826, 853, 958, 1240, 1538
NlaIV GGNNCC 7 cut(s) 265, 530, 531, 580, 689, 874, 1540
NmeAIII GCCGAG 2 cut(s) 1143, 1669
NmuCI GTSAC 4 cut(s) 83, 450, 971, 1262
NsbI TGCGCA 1 cut(s) 1447
NsiI ATGCAT 1 cut(s) 1281
NspI RCATGY 2 cut(s) 826, 853
PaeR7I CTCGAG 1 cut(s) 884
PciI ACATGT 1 cut(s) 822
PcsI WCGNNNNNNNCGW 6 cut(s) 201, 354, 446, 519, 1170, 1641
PctI GAATGC 1 cut(s) 1448
PdiI GCCGGC 2 cut(s) 927, 1047
PfeI GAWTC 5 cut(s) 332, 437, 550, 1342, 1592
PflFI GACNNNGTC 1 cut(s) 949
PkrI GCNGC 6 cut(s) 70, 460, 739, 924, 1402, 1525
PleI GAGTC 7 cut(s) 414, 608, 635, 958, 1121, 1372, 1654
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 7 cut(s) 414, 608, 635, 958, 1121, 1372, 1654
Ppu21I YACGTR 1 cut(s) 486
PpuMI RGGWCCY 2 cut(s) 493, 529
PscI ACATGT 1 cut(s) 822
PsiI TTATAA 1 cut(s) 1328
Psp124BI GAGCTC 2 cut(s) 240, 919
Psp5II RGGWCCY 2 cut(s) 493, 529
Psp6I CCWGG 1 cut(s) 291
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 7 cut(s) 265, 530, 531, 580, 689, 874, 1540
PspPI GGNCC 5 cut(s) 493, 529, 579, 687, 945
PspPPI RGGWCCY 2 cut(s) 493, 529
PspXI VCTCGAGB 1 cut(s) 884
PsuI RGATCY 1 cut(s) 1310
PsyI GACNNNGTC 1 cut(s) 949
RsaI GTAC 4 cut(s) 233, 663, 680, 890
RsaNI GTAC 4 cut(s) 232, 662, 679, 889
RseI CAYNNNNRTG 1 cut(s) 978
Rsr2I CGGWCCG 1 cut(s) 945
RsrII CGGWCCG 1 cut(s) 945
SacI GAGCTC 2 cut(s) 240, 919
SalI GTCGAC 1 cut(s) 638
SaqAI TTAA 2 cut(s) 1302, 1675
SatI GCNGC 6 cut(s) 69, 459, 738, 923, 1401, 1524
Sau3AI GATC 5 cut(s) 469, 648, 798, 1310, 1497
Sau96I GGNCC 5 cut(s) 493, 529, 579, 687, 945
SchI GAGTC 7 cut(s) 414, 609, 635, 959, 1121, 1373, 1655
ScrFI CCNGG 5 cut(s) 293, 893, 1124, 1385, 1586
SduI GDGCHC 5 cut(s) 240, 255, 318, 485, 919
SfaNI GCATC 3 cut(s) 1185, 1266, 1288
SfoI GGCGCC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 884
SgrAI CRCCGGYG 1 cut(s) 925
SinI GGWCC 5 cut(s) 493, 529, 579, 687, 945
SlaI CTCGAG 1 cut(s) 884
SmiMI CAYNNNNRTG 1 cut(s) 978
SmlI CTYRAG 1 cut(s) 884
SmoI CTYRAG 1 cut(s) 884
Sse9I AATT 4 cut(s) 359, 565, 1299, 1656
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 1 cut(s) 1199
SstI GAGCTC 2 cut(s) 240, 919
StyD4I CCNGG 5 cut(s) 291, 891, 1122, 1383, 1584
StyI CCWWGG 5 cut(s) 48, 175, 594, 954, 1314
TaaI ACNGT 7 cut(s) 76, 83, 140, 307, 637, 1093, 1550
TaiI ACGT 8 cut(s) 23, 198, 488, 575, 864, 1116, 1269, 1323
TaqII GACCGA 2 cut(s) 962, 1004
TasI AATT 4 cut(s) 359, 565, 1299, 1656
TauI GCSGC 2 cut(s) 925, 1403
TfiI GAWTC 5 cut(s) 332, 437, 550, 1342, 1592
Tru1I TTAA 2 cut(s) 1302, 1675
Tru9I TTAA 2 cut(s) 1302, 1675
TscAI CASTG 1 cut(s) 1357
TseFI GTSAC 4 cut(s) 83, 450, 971, 1262
TseI GCWGC 4 cut(s) 68, 458, 737, 1523
Tsp45I GTSAC 4 cut(s) 83, 450, 971, 1262
TspDTI ATGAA 2 cut(s) 616, 1253
TspGWI ACGGA 6 cut(s) 85, 193, 301, 755, 1502, 1575
TspRI CASTG 1 cut(s) 1357
Tth111I GACNNNGTC 1 cut(s) 949
VneI GTGCAC 1 cut(s) 481
VpaK11BI GGWCC 5 cut(s) 493, 529, 579, 687, 945
XceI RCATGY 2 cut(s) 826, 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 961
XhoI CTCGAG 1 cut(s) 884
XmiI GTMKAC 3 cut(s) 78, 639, 1376
XspI CTAG 1 cut(s) 1199
Zsp2I ATGCAT 1 cut(s) 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.